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Citations for Thermo Fisher :
1 - 50 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... then 200 μg of lysate was incubated with anti-Glu-Glu tag antibody (Thermo Scientific) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... with 2mM L-Glutamine (L-Glu: Thermofisher), 10% FBS and 1% P/S ...
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bioRxiv - Biophysics 2024Quote: ... and 1x Pen/Strep/Glu (Gibco 10378-016) (T-cell media) ...
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bioRxiv - Physiology 2023Quote: ... and 4 mM L-glutamine (L-Glu, Gibco) at 37° C with 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... 2 mM L-glutamine (L-Glu; Invitrogen, 25030024) and 50 ng/mL nerve growth factor (NGF ...
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bioRxiv - Immunology 2023Quote: ... which encodes for a C-terminal C-Direct tag containing a free thiol (cysteine) and an EPEA (Glu, Pro, Glu, Ala) purification tag (C-tag, Thermo Fisher Scientific). Vectors were transformed into yeast cells (Saccharomyces cerevisiae strain VWK18 (51)) ...
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bioRxiv - Microbiology 2022Quote: ... 50 μL of human glu-plasminogen (0.04mg/mL; Invitrogen) in Tris buffered saline was added to each well and mixed by pipetting ...
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bioRxiv - Microbiology 2021Quote: ... Z-DEVD-AMC substrate (catalog no. E13183, Invitrogen) in black ...
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bioRxiv - Biochemistry 2024Quote: ... and Gly-AMC (Thermo Scientific Chemicals, J65592.MA). Kinetic parameters for tripeptides LLL and LPL were obtained in the same way as described above using tripeptide-AMC conjugates (synthesized by GenScript) ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 1% (v/v) L-glutamate (L-Glu) (Invitrogen), 1% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... and endoproteinase Glu-C (MS grade) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Evolutionary Biology 2021Quote: Cells were cultured in RMPI-1640 with L-Glu (Gibco) supplemented with 15% FBS (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... AMC (7-amino-4-methylcoumarin) fluorescence reference standard (ThermoFisher Scientific, A191): Prepare a 100 mM stock in DMSO ...
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bioRxiv - Biochemistry 2022Quote: ... Hydroxyl-G (OH-primer) and 10-mer hydroxyl-G (10-mer OH-primer) (acquired from Thermo Fisher Scientific, USA) primers ...
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bioRxiv - Developmental Biology 2024Quote: ... The D/H-SG medium was supplemented with 2% B-27 (ThermoFisher), 20 ng/mL recombinant human fibroblast growth factor-basic (PeproTech ...
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bioRxiv - Microbiology 2022Quote: ... and Qubit Fluorometer (Thermo Fisher Scientific, OH, USA) were used to evaluate the quality of the gDNA ...
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bioRxiv - Microbiology 2022Quote: ... A NanoDrop spectrophotometer (Thermo Fisher Scientific, OH, USA), gel electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression of DOT1B-Myc-BirA* was induced by addition of 5 ng/ml tetracycline for 24 h and cells were further incubated for 24 h with 50 µM D-biotin (Invitrogen) and 5 ng/ml tetracycline ...
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bioRxiv - Bioengineering 2021Quote: ... Tetrabutylammonium hydroxide (TBA-OH) was purchased from Acros Organics. All other materials were purchased from Sigma-Aldrich.
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bioRxiv - Biophysics 2020Quote: ... 6% deoxy Big-CHAP (Affymetrix Anatrace, Maumee, OH, USA) and SecYEG in detergent (Protein to Lipid ratio of 1:50 ...
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bioRxiv - Bioengineering 2020Quote: ... Germany)] in a 5% CO2 incubator (Thermo Scientific, OH, USA) at 33°C.
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bioRxiv - Immunology 2022Quote: ... CA) were filled with rhIL-34 (0.42µg/h, i.e. 0.4 m/kg/d; Thermofisher, Waltham ...
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bioRxiv - Biochemistry 2024Quote: ... Magnesium chloride (MgCl2), sodium chloride (NaCl) and glutamic acid (Glu, 99%) were from Acros Organics. Histidine (His ...
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bioRxiv - Cell Biology 2022Quote: ... Suspension cells (THP-1) were first seeded for 6 h on poly-D-lysine (Gibco)-coated wells before starting the assay ...
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bioRxiv - Biochemistry 2021Quote: ... Ub-AMC hydrolysis was measured in a Varioskan Flash spectral scanning multimode reader (Thermo Fisher) by monitoring an increase of fluorescence excitation at 345 nm with an emission at 445 nm.
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bioRxiv - Microbiology 2020Quote: ... RNA was quantified using NanoDrop 2000 (ThermoFisher Scientific, Columbus, OH, USA).
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bioRxiv - Immunology 2021Quote: ... RNA was quantified using NanoDrop 2000 (ThermoFisher Scientific, Columbus, OH, USA).
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bioRxiv - Genomics 2023Quote: ... for 2 h and with 33 nM C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside; ThermoFisher D2893) for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... Controls were provided by cells treated with actinomycin D (Invitrogen, 1 µM; 1 h before labeling), RG7834 (0.05 µM ...
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bioRxiv - Developmental Biology 2020Quote: 5’-OH or fragmented RNAs were repaired using Polynucleotide kinase (Thermo scientific) following manufacturer instructions and incubated at 37°C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... used the ECL detection kit (Thermo Fisher Scientific ™, Cleveland, OH, USA) to visualizes specific bands.
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bioRxiv - Immunology 2020Quote: ... cells were re-suspended in complete RPMI (cRPMI) media consisting of RPMI-1640 with Glu-taMAX supplement (ThermoFisher Scientific) supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2022Quote: ... cells were maintained in a humidified 37° C incubator with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, MilliporeSigma) supplemented with 2 mM L-Glutamine (L-Glu, Gibco), 0.1 mM non-essential amino acids (NEAA ...
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bioRxiv - Biochemistry 2022Quote: ... cells were maintained in a humidified 37° C incubator with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, MilliporeSigma) supplemented with 2 mM L-Glutamine (L-Glu, Gibco), 0.1 mM non-essential amino acids (NEAA ...
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bioRxiv - Microbiology 2022Quote: ... and integrity were evaluated using a Nanodrop (Thermo Fisher Scientific, Cleveland, OH, USA), Qubit 3.0 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... and transcardially perfused with PBS followed by 4% paraformaldehyde (Affymetrix Inc., Cleveland, OH). Brains were postfixed in 4% paraformaldehyde for 24 hours at 4°C and then cryoprotected using sucrose in PBS for 16–24 hours ...
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bioRxiv - Microbiology 2020Quote: ... The absorbance was taken using Multiskan Go (Thermo Fisher Scientific, Columbus, OH, USA).
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed in Quantstudio 7 (Thermo Fisher Scientific, Columbus, OH, USA). All values were normalized with cycle threshold (Ct ...
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bioRxiv - Immunology 2020Quote: ... qRT-PCR was performed in Quantstudio 7 (Thermo Fisher Scientific, Columbus, OH, USA) using the cycle described as follows ...
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bioRxiv - Immunology 2021Quote: ... The absorbance was taken using Multiskan Go (Thermo Fisher Scientific, Columbus, OH, USA).
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in Quantstudio 7 (Thermo Fisher Scientific, Columbus, OH, USA). All values were normalized with the cycle threshold (Ct ...
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bioRxiv - Immunology 2024Quote: ... with the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Cleveland, OH, USA). Gene expression levels were calculated relative to Hprt expression using the ΔΔCt method and normalized to the indicated control samples ...
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bioRxiv - Cancer Biology 2024Quote: ... or RPMI 1640 for OHS (Life Technologist Invitrogen, Thermo Scientific, Waltham, MA, USA), supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... or RPMI 1640 for OHS (Life Technologist Invitrogen, Thermo Scientific, Waltham, MA, USA), supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... of “QTARKSTGGKAPRK” related peptides (1xAcetyl [K10]; 1xGln->pyro-Glu [N-Term]; 1xmethylations [K5]) using Qual Browser (Thermo xcalibur 4.1.50, Thermo Fisher Scientific) with ±15ppm width.
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bioRxiv - Immunology 2024Quote: Isolated LECs were placed in complete T-cell media (RPMI + 10% FBS + 1% L-Glu + 100U/ml Pen/Strep + 1% MEM NEAA + 1% Na-Pyruvate + 0.1% 2-mercaptoethanol) (Gibco, USA) with PBS or LPS (1µg/ml ...
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bioRxiv - Genetics 2023Quote: ... were grown on 10-mm coverslips previously coated with poly-D-lysine (0.1 mg/mL, 1 h, 37 °C, Gibco) placed in a well on a 24-well plate ...
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bioRxiv - Developmental Biology 2020Quote: ... and transcardially perfused with PBS followed by 4% paraformaldehyde (PFA; Affymetrix Inc., Cleveland, OH). Tissues were post-fixed in 4% PFA overnight at 4°C and then cryoprotected by submersion in 30% sucrose in PBS for 16–24 hours then cryosectioned for immunofluorescence and Hematoxylin (Fisher Chemical ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR products were cleaned used the ExoSAP-IT protocol (USB-Affymetrix, Cleveland OH, USA) and sequenced at Eurofins Genomics on an ABI prism sequencing platform (Louisville ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... Hsp90’s phosphorylation sites were identified using Glu-C and trypsin digestion and liquid chromatography-mass spectrometry on a QExactive plus mass spectrometer (Thermo Fisher).