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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for Recombinant Mouse Acvr1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... followed by Fc-Block step with anti-mo CD16/CD32 (Invitrogen, Cat# 14-01061-86) and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD ...
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bioRxiv - Physiology 2024Quote: NIH-3T3 fibroblasts (3T3-FB, 1.0×104 cells) were cultured in 10 % FCS/DMEM (Gibco, Life Technologies ...
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bioRxiv - Microbiology 2024Quote: Intact VHH-Fc proteins were separated on an Ultimate 3000 HPLC system (Thermo Fisher Scientific) online connected to an LTQ Orbitrap Elite mass spectrometer (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2023Quote: ... IgGs were isolated from sera using 2 mL CaptureSelect IgG-Fc resin slurry (Thermo Scientific) per 1 mL rabbit sera ...
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bioRxiv - Cell Biology 2023Quote: ... Dissociation was stopped by addition of 2 volumes of DMEM-F12 with 10% FCS (GIBCO) and cells were counted ...
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bioRxiv - Genetics 2024Quote: ... We measured the absorbance at 492 nm in a microplate reader (Thermo Scientific Multiskan FC). Plotting of graphs and statistical analyses were conducted with GraphPad Prism 8.4.2.
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bioRxiv - Immunology 2024Quote: ... The plate was analyzed every hour using a Multiskan FC plate reader (Thermo Fisher Scientific), and absorbance was measured at 620 nm.
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% FCS and 1 U/mL Penicillin-Streptomycin (10 000 U/mL, Gibco) in 96 well round-bottom plates ...
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bioRxiv - Cell Biology 2023Quote: ... MDA-MB-231 cells were grown in DMEM/F12 with 10% FCS (Thermo Fisher Scientific) and 100 U μL/mL penicillin and 100 μL/mL streptomycin ...
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bioRxiv - Immunology 2023Quote: TAP2 deficient CHO cells were cultured in complete RPMI (Invitrogen, 2mM glutamine and 10% FCS) and stably-transfected with >10g plasmid DNA using poly-L-ornithine and a 30% DMSO shock ...
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bioRxiv - Microbiology 2023Quote: ... The absorbance was determined at 450 nm using a microplate reader (Thermo Scientific Multiskan FC). All experiments were carried out in four biological replicates.
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions were treated with anti-CD16/32 (Fc block) (14-9161-73, ThermoFisher) and stained with the live/dead marker ethidium monoazide bromide (ThermoFisher #E1374 ...
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bioRxiv - Immunology 2023Quote: ... was measured at 180 minutes (590nm excitation and 620nm emission) with Multiskan FC (Thermo Scientific) [33 ...
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bioRxiv - Immunology 2023Quote: ... was measured at 180 minutes (544nm excitation and 590nm emission) with Multiskan FC (Thermo Scientific) [31].
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% L-glutamine (Gibco™ 25030-024). The human nasal septum epithelial cell line RPMI 2650 (ATCC CCL-30 ...
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bioRxiv - Cancer Biology 2023Quote: ... The data was immediately collected by Multiskan™ FC Microplate Photometer (Thermo Fisher Scientific, USA) at 450 nm ...
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bioRxiv - Plant Biology 2023Quote: ... Absorbance at 340 nm was monitored continuously with a spectrophotometer (MULTISKAN FC; Thermo Fisher Scientific) to detect NADH production.
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bioRxiv - Developmental Biology 2023Quote: ... with 10% FCS (Biosera, #FB-1280/500) and 2% glutamate/pyruvate (100mM sodium pyruvate, Invitrogen #11360-039 ...
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bioRxiv - Cancer Biology 2023Quote: Hap1 cells (Horizon Discovery) were maintained in IMDM supplemented with GlutaMAX and 10% FCS (Gibco). Cells regularly tested negative for mycoplasma contamination.
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bioRxiv - Physiology 2023Quote: ... and blocked on ice for 20 minutes with Fc Block anti-CD16/CD32 (Thermo Fisher). Then ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Capricorn) and 1% penicillin/streptomycin (Gibco). Huh 7.5 cells were maintained in complete DMEM medium supplemented with non-essential amino acids.
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bioRxiv - Microbiology 2023Quote: ... OD600 measurement in a microplate reader (Multiskan™ FC, Thermo Scientific, Etten-Leur, the Netherlands) where performed ...
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bioRxiv - Microbiology 2023Quote: ... Optical density was measured at 450 nanometer using a Multiskan FC machine (Thermo Scientific, USA) in combination with Mikrowin (software version 5.09 ...
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bioRxiv - Immunology 2023Quote: ... Pheonix cell and LS174T cells were cultured in complete DMEM (Gibco, 10% FCS, 2mM GlutaMAX).
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bioRxiv - Immunology 2023Quote: ... Samples were then incubated with an Fc receptor binding inhibitor polyclonal antibody (Thermo Fisher Scientific), before staining with a surface antibody cocktail (Supplementary Table 1) ...
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bioRxiv - Immunology 2024Quote: ... Absorbance was detected at 450 nm by the Multiscan FC (Thermo Fisher, Waltham, MA, USA).
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bioRxiv - Immunology 2024Quote: ... + 10% FCS + 1% GlutaMAX + penicillin-streptomycin (P/S, 12.8 U/mL, Gibco, cat.15140-122) supplemented with 10% DMSO at -150°C ...
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bioRxiv - Immunology 2024Quote: ... followed by absorbance measurements at 450nm using a Multiskan FC Microplate Photometer (51119000; Thermo Scientific). As a positive control ...
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bioRxiv - Plant Biology 2024Quote: ... and reading absorbance at 620 nm in a photometer (Multiskan FC, Thermo Scientific, United States) and using a glucose standard curve to compare the colorimetric response of the samples ...
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bioRxiv - Immunology 2024Quote: ... containing 10% FCS (Biochrome, Cat. S0615-500ML) and 1% Penicillin/ Streptomycin (Thermo Fisher, Cat. 7001592). After sorting ...
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bioRxiv - Biophysics 2021Quote: ... Control Drosophila melanogaster biotin-tagged kinesin-1 motors were labeled with streptavidin-conjugated Qdot 655 (Life Technologies, Carlsbad, CA) at a 1:4 motor:Qdot ratio as previously described [16 ...
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bioRxiv - Cell Biology 2020Quote: ... K16-flag protein (C-terminally tagged) was made using the pcDNA™3.1 Directional TOPO™ Expression Kit (ThermoFisher) using primers for the mouse Krt16 gene with flag peptide sequence on the 3’ end (Supplemental Table 1).
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bioRxiv - Cell Biology 2020Quote: ... The pDONR201 clones were transferred into GFP or SFB tagged DEST vectors using the Gateway LR cloning system (Invitrogen). KDM5AΔPID deletion mutant was generated by PCR in pDONR201 vector following standard cloning method and then subcloned into Gateway destination vector derived from pcDNA5/FRT/TO plasmids containing the NLS and SFB- or GFP-tagged in N-terminal ...
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bioRxiv - Cell Biology 2020Quote: ... GFP or luciferase tagged versions of the proteins were generated using the Gateway recombination cloning procedure (Invitrogen, Carlsbad, CA) and custom-made vectors.
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bioRxiv - Molecular Biology 2021Quote: GPI-APs were HA-tagged by cloning their coding cDNA amplified from the human cDNA library or from Invitrogen plasmids ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells grown on 100-mm dishes were cotransfected with Flag-tagged ASK1_KR and PKA or empty vector using Lipofectamine 2000 (Invitrogen). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Transferrin (Tf) and Epidermal Growth Factor (EGF) tagged with Alexa Fluor 488 was obtained from Invitrogen (T13342 and E13345). Dako fluorescent mounting medium from Agilent (S3023 ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
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bioRxiv - Genomics 2020Quote: ... 20.25 μg 3×Flag-tagged dCas9 plasmid and 20.25 μg sgRNA plasmid were transfected into cells using Lipofectamine 3000 (Invitrogen). Two biological replicates were performed for each condition ...
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bioRxiv - Cancer Biology 2021Quote: Control MCF-10A cells or cells expressing HA-tagged PEAK3 were cultured in -EGF medium (DMEM/F-12 (Gibco) supplemented with 0.5◻μg/ml hydrocortisone (Sigma) ...
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bioRxiv - Biophysics 2021Quote: Full-length HA-tagged p75NTR and full-length Myc-tag TRADD were expressed from a pcDNA3 vector backbone (Invitrogen). The HA-p75E345A ...
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bioRxiv - Biochemistry 2021Quote: N-terminal GST-tagged MBNL1 (amino acids 1-260)59 was recombinantly expressed using BL21 Star (DE3) cells (Invitrogen) and protein expression induced using 0.5 mM IPTG at an OD600 = 0.6-0.7 for 2 hours at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... and the tagged SΔTM was purified by affinity chromatography using Ni-NTA agarose beads (Invitrogen™, Waltham, MA, USA) and size exclusion chromatography as described above.
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bioRxiv - Microbiology 2021Quote: ... bacteria were tagged by Gfp or stained by cell membrane stain FM4-64 (10 µM final concentration, Molecular Probes) for 2 mins in the dark (without flow) ...
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bioRxiv - Cell Biology 2021Quote: ... Stable cell lines expressing BioID tagged genes of interest were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5/FRT/TO construct and Flipase expressing pOG44 plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids for expressing tagged proteins in S2 cells were generated by cloning coding sequences into pEntr/SD (Thermofisher, K242020), and recombining the coding sequences into pAc5 promoter destination vectors (pAWM and pAWF) ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...