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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Recombinant Mouse Acvr1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... This mix was added to 50 µl pre-washed His-Pur magnetic Nickel beads (Thermofisher Scientific) and incubated for 1 h at 4 °C on a rotor ...
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bioRxiv - Microbiology 2020Quote: ... NR-52285) using the primers in Table II and cloned into pCDNA-V5/His TOPO (Invitrogen). We introduced a FLAG tag to JUNV GP by PCR amplifying it from a previously described expression plasmid (58 ...
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bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with indicated plasmids or blank pMT-V5-His vector (Invitrogen, Cat# V412020) using the Effectene transfection reagent (Qiagen ...
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bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
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bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
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bioRxiv - Cell Biology 2019Quote: ... The his-tag was removed by cleavage overnight with AcTEV™ protease (Invitrogen, Carlsbad, California, USA) at 4ºC according to the provider’s protocol.
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bioRxiv - Immunology 2021Quote: ... with 10% FBS (Nucleus Biologics, #AU FBS-500ml L1 HI) and 1% Penicillin-Streptomycin (Gibco, #15140122) and transferred to a new tube ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts were released by Bam-HI and Eco-RI digestion and ligated to pENTR4 (Invitrogen; previously digested with the same restriction enzymes) ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid CassetteAv2_pBAD contains the CRISPR03 array cloned into the pBAD/Myc–His B backbone (Life Technologies).12 It was used to prepare CRISPR DNA substrates by PCR with primers MMB1Lead40-5 and MMB1crisp3-r1 using Phusion High-Fidelity DNA polymerase according to the manufacturer’s protocol (New England Biolabs or ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant fraction was incubated with 2 ml Ni-NTA His-Pur resin (Thermo Fisher, PI88222) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen, 10103D). After one wash with NLB buffer ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with an anti-His Rabbit primary antibody (Thermo Scientific, Massachusetts, United States) (1:1000) ...
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bioRxiv - Microbiology 2023Quote: ... brucei rRAP1-His at 10 µM was incubated with 10 mM SYPRO Orange dye (ThermoFisher Scientific), filtered before use with a 0.22 μm sterile filter (Fisher scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Neuroscience 2023Quote: ... The purified His-TEV-FAM19A5 proteins were digested overnight at 30°C with AcTEV protease (Invitrogen) to remove the His-tag and TEV sequence ...
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bioRxiv - Neuroscience 2024Quote: ... The buffer was then exchanged to the wash solution (10 % Hi-DiTM Formamide (Thermofisher scientific; 4311320) in 2x saline sodium citrate (SSC) ...
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bioRxiv - Physiology 2021Quote: ... mouse anti-mouse GAPDH (Thermo Fisher, #AM4300), rabbit-anti-mouse LSD1 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... T1249-Fc were expressed by transient transfection in 293-6E (CNRC) or Expi293 (ThermoFisher Scientific) cells and purified from transfected cell supernatants using a HiTrap MabSelect SuRe column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... absorbance at 405 nm was measured in a MultiSkan FC microplate reader (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Both compensation controls and samples were washed twice with 200μL FACS buffer (2% FCS (Gibco) in PBS (Gibco)) ...
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bioRxiv - Immunology 2022Quote: ... Digestion was quenched by adding PBS supplemented with 2% FCS and 5 mM EDTA (Gibco). Cell suspensions were obtained after filtration over the 70-μm cell strainer ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Neuroscience 2020Quote: ... 10% FCS and 100 units / ml of penicillin and 100 μg / ml of streptomycin (Gibco). U87MG ...
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-rabbit IgG Fc secondary antibody (Invitrogen, Cat. No. 31463).
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bioRxiv - Molecular Biology 2020Quote: ... Absorbance at 570 nm was measured using a spectrophotometer (Multiskan FC Microplate Photometer, Thermo Fisher). A standard curve was generated using standard glycogen solution in the reaction ...
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at OD450 nm using a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at OD450 nm using a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Cells were infected with the specified viruses and MOIs in DMEM without FCS (Life Technologies) for 1h at 37.0°C (Cts24 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco) for 3 hours before fixation for 15 minutes (3.7% FA and 0.5% triton X-100) ...
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bioRxiv - Molecular Biology 2022Quote: ... and then blocked in PBS supplemented with 20% foetal calf serum (FCS, Thermo Fisher Scientific) and 20% normal goat serum (NGS ...
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bioRxiv - Immunology 2022Quote: ... and were used after freezing in Fetal Calf Serum (FCS) (Invitrogen, Waltham, Massachusetts, United States) containing 10% DMSO (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... Absorbance at 405 nm was read on a Multiskan FC plate reader (Thermo Scientific, USA).
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bioRxiv - Biochemistry 2020Quote: ... SKOV3-cells were cultured in McCoy’s 5A Medium supplemented with 10% FCS (Thermo Fisher Scientific). HROC24-cells were cultured in DMEM/Ham’s F12 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 1mM sodium pyruvate and 3.7 g/L NaHCO3 and supplemented with 15% FCS (Gibco, A3160801), 10 mM HEPES (Gibco ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin (P/S; Thermo Fisher) in a humidified atmosphere at 37°C with 5% CO2 ...
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bioRxiv - Physiology 2019Quote: ... Cells were kept in DMEM supplemented with 10% tetracycline-free Hyclone™ FCS (Fisher Scientific) and the above-mentioned antibiotics.
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice following staining and resuspended in FC buffer containing 7AAD (Life Technologies) or FVS700 (BD Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at 450 nm by a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... supplemented with 4% FCS and 0.75% sodium bicarbonate containing 0.5% low melting agarose (Invitrogen, USA). The overlay was allowed to solidify at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% fetal calf serum (FCS) and 2mM L-glutamine (all from Life Technologies). HEK293T cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% foetal calf serum (FCS) and 1mM Sodium Pyruvate (all from Life Technologies). DLD-1 and COLO205 cells were maintained in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Biochemistry 2020Quote: ... The absorbance was measured with a Multiscan™ FC microplate photometer (Thermo Fisher Scientific, USA). Cell viability is presented as the percentage of control (untreated cells) ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% inactivated fetal calf serum (FCS) (Cat: a3520501 Thermo Fisher Scientific Inc, USA), an 1% antibiotic mixture (Cat ...
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bioRxiv - Immunology 2020Quote: ... DMEM/HAM’s F12 (FAD) medium with low Ca2+ (50 µM) supplemented with 10% FCS (Gibco) treated with chelex 100 resin (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... When needed neutrophils were cultured in RPMI 1640 containing 10% fetal calf serum (FCS, Gibco), 2 mM L-glutamine ...
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bioRxiv - Immunology 2020Quote: ... Fc-fusion proteins were expressed via transient transfection of suspension-cultured CHO-S (Invitrogen/ThermoFisher) cells using the Mirus Bio ...
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bioRxiv - Immunology 2020Quote: ... Fc-fusion proteins were expressed via transient transfection of suspension-cultured CHO-S (Invitrogen/ThermoFisher) cells using the Mirus Bio ...