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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for Recombinant Human ITGAV & ITGB5 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Assessment of Human Renal Transporter Based Drug-Drug Interactions Using Proximal Tubule Kidney-ChipbioRxiv - Cell Biology 2022Quote: ... Heat inactivated fetal bovine serum (HI FBS) and trypan blue were procured from Gibco Life Technologies (Waltham ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Life Technologies unless indicated), 100 U/mL penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... 3 μg of mammalian expression vector plasmids based on pEF1α V5 His C (Invitrogen) encoding codon-optimized gH ...
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bioRxiv - Microbiology 2019Quote: Qst was fused to a His-tag using the pEXP5-CT/TOPO vector (Invitrogen) following the TA-cloning protocol provided by the manufacturer ...
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bioRxiv - Immunology 2019Quote: ... 1xPen/Strep/Glu and 10% ultralow IgG HI-FBS (Thermo Fisher Scientific, Waltham, MA), and pelleted at 350xg for 5min ...
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bioRxiv - Biochemistry 2019Quote: ... A full length cDNA of Matriptase constructed in pcDNA3.1-V5-His (Thermo Fisher Scientific) was provided by Dr ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the blot was incubated with HRP conjugated anti-His antibody (Invitrogen, LOT 1902132) at 1:5000 dilutions for 16h at 40 C ...
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bioRxiv - Microbiology 2020Quote: ... 1U Platinum High Fidelity (Hi-Fi) proof reading Taq polymerase (Invitrogen, Carlsbad, CA, USA). Two colonies of each G ...
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bioRxiv - Microbiology 2020Quote: ... using the primers shown in Table II and cloned into pcDNA-V5/His (Invitrogen). pCAGGS-EboGP-V5 (strain Zaire 1976 Mayinga ...
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bioRxiv - Microbiology 2022Quote: Pull-down assays were carried out using Dynabeads His-tag Isolation and Pulldown (Invitrogen). The 6His-tagged soluble pilin domains were used as bait ...
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bioRxiv - Biochemistry 2022Quote: ... and cultured in house) were grown to 70% confluency in DMEM-Hi glucose (Gibco) supplemented with 10% heat-inactivated foetal bovine serum (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were digested and ligated into pcDNA3.1 myc-His B expression vectors (Invitrogen), with a 3xFlag epitope tag also fused to the N-terminus of Ptchd1 ...
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bioRxiv - Biochemistry 2022Quote: ... redundant anti-His antibody was washed away and 15 μL chemiluminescence substrate (Thermo Scientific) was added onto each well to generate chemiluminescence signal ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco™ Cat. #10438026) and 1% antibiotic/antimycotic (Gibco™) ...
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bioRxiv - Plant Biology 2022Quote: ... and purified by Dynabeads™ His-Tag Isolation and Pulldown (ThermoFisher, Catalog number 10103D). DA1 swapped protein ubiquitylation reactions used E1 ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 30 minutes on ice with His-Pur Ni-NTA resin (Thermo Scientific), packed to a height of approximately 5 cm in polypropylene columns ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and His-p115 were affinity purified using Ni-NTA HisPur beads (Thermo Fisher Scientific) from crude E ...
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bioRxiv - Microbiology 2023Quote: ... A standard curve of 10-fold dilutions of plasmid pAc5.1-V5-His-A (Invitrogen) containing the DCV ORF-2 sequence was used to convert Ct values to genome copy numbers.
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bioRxiv - Evolutionary Biology 2022Quote: ... and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (Thermo Fisher Scientific #MA1-21315) diluted in 5% milk in PBS-T (1:500) ...
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bioRxiv - Molecular Biology 2022Quote: ... Junction PCR was performed with Extensor Hi-Fidelity PCR Master Mix (Thermo Fisher Scientific) in a 20 μL reaction using 500 nM of target-specific primers and the following cycling conditions ...
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bioRxiv - Immunology 2023Quote: ... M4 cells are maintained in DMEM/F12 media with 5% HI HS (Gibco #26050088).
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bioRxiv - Neuroscience 2023Quote: ... and subcloned into the pcDNA™3.1/myc-His(−)A vector (Thermo Fisher Scientific). We accidentally obtained the pcDNA™3.1/myc-His(−)A-(G4C2)9 vector at this step ...
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bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... HI samples were incubated with 1 mL of IgG Fc Capture Select (Thermo Fisher) resin at 4C for 20 to 48 h to bind IgG ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then heated with Novex Hi-Density TBE sample buffer (Invitrogen LC6678) at 95°C for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS) (10082147, Thermo Fisher Scientific) and 1% penicillin/streptomycin 1000 U/mL (Pen/Strep ...
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bioRxiv - Developmental Biology 2024Quote: ... wild type Cic and mutant variants were subcloned into pMT-V5-His vector (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... Biotinylated BG505 SOSIP timers were immobilized on Streptavidin-coated 96-well plates (Thermo Fisher Scientific) at a concentration of 5 μg/mL in blocking buffer (1% BSA +1% goat serum in TBS-T ...
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bioRxiv - Neuroscience 2021Quote: The secondary antibodies used were: biotinylated anti-mouse IgG (Invitrogen cat#62-6540, 1:1k), Alexa Fluor 488 donkey anti-rabbit IgG (Invitrogen cat#A21206 ...
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bioRxiv - Molecular Biology 2020Quote: ... duplicate 20 μg RNA samples were biotinylated using Click-iT Nascent RNA Capture reagents (ThermoFisher) and purified on Dynabeads MyOne Streptavidin T1 magnetic beads (ThermoFisher ...
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bioRxiv - Bioengineering 2019Quote: Purified Ana GVs were biotinylated using EZ-Link Sulfo-NHS-LC-biotin (Thermo Fisher Scientific) according to the protocol described previously48 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Biotinylated taxol staining was detected by streptavidin-R-Phycoerythrin (PE) (1:1000, Invitrogen, S-866) in yellow ...
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bioRxiv - Immunology 2019Quote: ... All probes were biotinylated at the 3’ end with an 18-carbon spacer arm (Invitrogen). 20 million cells were cross-linked by 1% formaldehyde ...
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bioRxiv - Immunology 2019Quote: Anti-CD3ε and anti-CD28 antibodies were biotinylated using EZ-link sulfo-NHS biotin (ThermoFisher). Streptavidin-coated polystyrene beads 6-8 μm in diameter (Spherotech ...
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bioRxiv - Biochemistry 2019Quote: ... The biotinylated peptides are retrieved by incubating the lysate with Dynabeads MyOne Streptavidin C1 (Invitrogen) 1h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... followed by biotinylated rabbit anti-goat IgG (1/200 dilution; Catalogue number 31732 Invitrogen, UK) with streptavidin Alexafluor 647 (1/200 ...
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bioRxiv - Biophysics 2020Quote: Biotinylated 5 kb long DNA was synthesized by PCR using ΦX174 RF1 DNA (ThermoFisher Scientific) as a template and oligonucleotides 5’ biotin-CGAAGTGGACTGCTGGCGG-3’ and 5’-CGTAAACAAGCAGTAGTAATTCCTGCTTTATCAAG-3’as primers ...
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bioRxiv - Bioengineering 2021Quote: ... The biotinylated baits were immobilized on 96-well streptavidin-coated plates (Thermo Fisher Scientific 15500). The wells were then washed three times with wash buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated cells were labeled with 17.5 ug/ml Streptavidin magnetic beads (#21344, Thermo Fisher scientific) for 1.5 hours followed by 3 x 15 min magnetic sorting with PBS + 0.5% glucose washes in between ...
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bioRxiv - Systems Biology 2021Quote: The biotinylated bait DNA was then bound to equilibrated Dynabeads MyOne Streptavidin C1 beads (Invitrogen). Beads were equilibrated by washing three times with 1x B&W buffer (5 mM Tris Cl ...
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bioRxiv - Cell Biology 2022Quote: TN41 was biotinylated with an EZ-LinkTM Sulfo-NHS-LC-Biotinylation kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and CD19.CAR-T cells were labeled with biotinylated anti-Fab antibodies (ThermoFisher Scientific, 31803). The co-culture was then mixed with CELLection Biotin Binder (ThermoFisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... After incubation with a secondary biotinylated goat anti-rabbit (diluted 1/1000, A24541, Life Technologies), chromogranin A was revealed with Alexa Fluor 568-conjugated streptavidin (S11226 ...
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bioRxiv - Immunology 2020Quote: ... PfAMA1 and PfMSP1 were biotinylated (EZ-link Sulfo-NHS-LC-Biotinylation kit; Thermo Fisher Scientific) using a 1:1 ratio of biotin to protein and once the molar amount of biotinylated protein was measured using a Western blot ...
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bioRxiv - Neuroscience 2020Quote: ... 4% biotinylated dextran-conjugated tetramethylrhodamine (3000 mw, micro-ruby, Molecular Probes; Invitrogen, Eugene, OR, USA) in 0.2 M KAc ...
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bioRxiv - Neuroscience 2020Quote: ... 4% biotinylated dextran-conjugated tetramethylrhodamine (3000 mw, micro-ruby, Molecular Probes; Invitrogen, Eugene, OR, USA) in 0.2 M KAc ...
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bioRxiv - Immunology 2020Quote: ... Purified antigens were biotinylated using an EZ-Link Sulfo-NHS-LCBiotinylation kit (Thermo Fisher Scientific) using a 1:1.3 M ratio of biotin to Ag ...
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bioRxiv - Developmental Biology 2019Quote: ... and biotinylated anti-Avidin (1:100) together with Alexa streptavidin 594 (1:500, Molecular Probes); nuclei were counterstained with DAPI ...