Labshake search
Citations for Thermo Fisher :
2351 - 2400 of 7360 citations for HDGF2 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... human IL-8 (Invitrogen™ 88808677), human IL-1beta (Invitrogen™ 88726177 ...
-
bioRxiv - Immunology 2024Quote: ... + 50 ng/ml human EGF (Gibco) + 25 ng/ml human FGF10 (Stemcell Technologies ...
-
bioRxiv - Immunology 2023Quote: ... anti-human CCR7 (clone 3D12, Invitrogen), streptavidin (BioLegend) ...
-
bioRxiv - Biophysics 2023Quote: ... 5 µg/mL human insulin (Gibco), 2 mM L-glutamine (Corning) ...
-
bioRxiv - Biophysics 2021Quote: A commercial HEK cell suspension cell line optimized for protein expression Expi293-F (Cat. No. A14635, Gibco Life Technologies, Carlsbad, California, USA) was used according to manufacturer’s instruction ...
-
bioRxiv - Microbiology 2022Quote: ... Transfection was performed in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... VA) and HEK-293T (#CRL-11268; ATCC) cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; #11965118; Gibco – Thermo Scientific, Waltham, MA) supplemented with fetal bovine serum (FBS ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293T/17 cells were purchased from ATCC (Cat. No. CRL-11268) were maintained in DMEM (Thermo Fisher Scientific, Cat. No. MT10013CV) with 10% fetal bovine serum and 1% Pen-Strep ...
-
bioRxiv - Immunology 2020Quote: ... The purified plasmid DNA (both heavy and light chain) was transfected into a 30mL culture of HEK Expi293 cells (Thermo Scientific Expi293). Harvesting the culture after 5 days ...
-
bioRxiv - Microbiology 2020Quote: ... All the ACE2 variants were expressed in HEK 293F cells by transient transfection using Opti-MEM (Gibco-Thermo Fisher Scientific, Waltham, MA). After incubation for 4 days at 37°C with 5.5% CO2 ...
-
bioRxiv - Microbiology 2020Quote: ... All the ACE2 variants were expressed in HEK 293F cells by transient transfection using Opti-MEM (Gibco-Thermo Fisher Scientific, Waltham, MA). After incubation for 4 days at 37°C with 5.5% CO2 ...
-
bioRxiv - Microbiology 2021Quote: ... VA) and HEK-293T (#CRL-11268; ATCC) cells were cultivated in Dulbecco’s Modified Eagle Medium (DMEM; #11965118; Gibco – Thermo Scientific, Waltham, MA) supplemented with fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2021Quote: The two lentiviral particles of the barcode and sgRNA vectors were individually transfected into HEK-293FT cells (Cyagen, no. HEKFT-30001) with lentivirus packaging plasmids using Lipofectamine 2000 (ThermoFisher, no. 11668027). To obtain the viral particles ...
-
bioRxiv - Genomics 2022Quote: ... HEK-293T cells were grown on a 10 cm dish up to 70-80% confluency in HEK-media (KO-DMEM (Themro Fisher Scientific, 10829018), 10% fetal bovine serum (FBS ...
-
bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
-
bioRxiv - Microbiology 2023Quote: ... scrambled or PUS1/PUS7ko HEK-293Ts were transfected with 2 μg PAN and 2 μg ORF57-eGFP plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
-
The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... in the presence of the selection antibiotic when required (HEK-293T-FLAG-CB1R; zeocin at 0.22 mg/mL, Thermo Fisher Scientific #R25001), and were periodically checked for the absence of mycoplasma contamination ...
-
bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with psPAX2 (addgene plasmid #12260) and pMD2.G (addgene plasmid #12259) into HEK 293T cells using TurboFect (Thermo Fisher Scientific). Viral supernatant was collected on day 3 and 4 after transfection ...
-
bioRxiv - Cell Biology 2022Quote: ... this mixture was added dropwise to confluent HEK-293T cells (ATCC, CRL-3216) in a T175 flask containing 35 mL DMEM media (Gibco #11965-092) and supplemented with 1mM sodium pyruvate (Gibco #11360-070) ...
-
bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
-
bioRxiv - Systems Biology 2024Quote: ... VP4:VP2-complemented CVB3 A67G was prepared by transfecting HEK-293T cells with equal molar ratios of pcDNA CVB3 A67G and pcDNA3 VP4:VP2 using Lipofectamine 2000 (Thermo Fisher, 11668019). Viral supernatants were concentrated by ultracentrifugation (100,000 rcf for one hour at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Wild type TGM2 and the TGM2-C277A mutant were overexpressed in HEK 293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: HEK-293T (CRL-11268) cells and PK-15cells (CCL-33) were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, Carlsbad, CA USA) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2024Quote: ... was placed at the beginning of the scFc sequence to facilitate protein secretion.54 The plasmid was transfected into HEK 293F cells (Thermo Fisher Scientific). After 88 h of incubation ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was produced in HEK-293T cells using either the Trans-Lentiviral ORF Packaging Kit (Dharmacon/Horizon Discovery) or Lipofectamine 3000 (Thermo Fisher Scientific). Viral supernatants were collected 72 hours (hrs ...
-
bioRxiv - Immunology 2021Quote: ... Purified digested DNA fragments were cloned into human Igγ1- and Ig κ-/ Igλ-expressing vectors 38 and recombinant IgG1 antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described 39 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in Dulbecco’s minimum essential medium (DMEM/F12; Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Biochemistry 2020Quote: ... Suspension Expi293 cells were grown to 2.5 million cell density and transiently transfected with ADAMs protease expression vectors using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific, # A14525). Medium was harvested after 6-7 days and protein was purified by Ni-NTA chromatography and assessed for quality and activity.
-
bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDs was harvested 96 h after infection ...
-
bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDwt or RBDmut was harvested 96 h after infection ...
-
bioRxiv - Biophysics 2022Quote: ... The PEAK3-1xFLAG construct was transfected into 30 ml of Expi293F culture at a cell density of 4.0 × 106 cells/ml using ExpiFectamine 293 transfection reagent (Thermo Fisher Scientific Cat #A14524) according to the manufacturer’s protocol at 1 μg DNA / 1 ml ...
-
bioRxiv - Synthetic Biology 2023Quote: ... supernatant was harvested from 293S GnTi- TetR sender A’-C (senders 1) and 293S GnTi- TetR sender Γ’-A’ (senders 2) cells that have been continuously cultured in DMEM (41966; Thermo Fisher Scientific) supplemented with 10% (v/v ...
-
bioRxiv - Immunology 2023Quote: Secretion levels from plasmid DNA constructs were evaluated following transient transfections of Expi293F™ cells using the ExpiFectamine™ 293 Transfection kit (Gibco, LifeTechnologies). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd- SARS-CoV-2-BA.5-S ...
-
bioRxiv - Bioengineering 2023Quote: ... 2×106 HEK-293 cells were electroporated with the pCMV3 vector encoding CD123 wildtype or its variants using the Neon Transfection System (ThermoFisher; 1100V, 20ms, 2pulses). To generate stable cell lines Geneticin G418 (50mg/ml ...
-
bioRxiv - Immunology 2023Quote: ... After 5 days the cells were spun down at 80g for 7 minutes at 20°C and media were changed to 30 mL prewarmed Freestyle™ 293 Expression Medium with 0,5% Penicillin-Streptomycin (Thermofisher, cat no: 15140122), vortexed vigorously for 20 seconds to break cell clumps ...
-
bioRxiv - Biochemistry 2024Quote: ... Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific). Flp-In 293 cells were cultured in DMEM supplemented with 10% FBS ...
-
bioRxiv - Immunology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd-SARS-CoV-2-BA.5-S ...
-
bioRxiv - Immunology 2024Quote: ... Mutant CRP protein was produced by transfecting 25 µg plasmid DNA into Expi293F™ cells using the ExpiFectamine™ 293 Transfection Kit (ThermoFisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... DNA samples containing the extension products were added to Hi-Di formamide (Applied Biosystems, Foster City, CA). The mixture was incubated for 5 min at 95°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Air-liquid interface medium consisted of 50% BEGM and 50% hi-glucose minimal essential medium (DMEM, Gibco) containing 100 nM additional retinoic acid (Sigma Aldrich) ...
-
bioRxiv - Microbiology 2021Quote: ... parahaemolyticus POR1 genomic DNA and inserted into the multiple cloning site (MCS) of pBAD/Myc-His (Invitrogen) vector containing a kanamycin-resistance cassette 25 (in-frame or out-of-frame with the C-terminal Myc-His tag ...
-
bioRxiv - Genetics 2020Quote: ... The human PROS1 cDNA (GenBank NM_000313.4) was PCR-amplified from HepG2 cDNA and cloned into pcDNA3.1/myc-His(−) plasmid (Invitrogen). The PCR amplification of PROS1 from c.-44 to c.2028 was performed using Phusion High Fidelity (HF ...
-
bioRxiv - Developmental Biology 2021Quote: ... The others were TA-cloned to pcDNA3.1/V5-His TOPO TA vectors (Cat. No. K4800-01, Invitrogen). HEK293T cells were cultured in DMEM medium with 10% FBS in 5% CO2 at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The araC gene and araBAD promoter were PCR-amplified from pBAD/His/lacZ (Invitrogen, Carlsbad, CA, USA). The lac promoter and inhibitory unit (iUnit ...
-
bioRxiv - Developmental Biology 2020Quote: ... The template was prepared using the Ion PI Hi-Q OT2 200 kit (Thermo Fisher Scientific, UK). Sequencing was performed using the Ion PI Chip Kit v3 (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... using the following primary/secondary antibodies: rabbit anti-6x-His Tag (ThermoFisher Scientific PA1-983B, 1:10,000), mouse monoclonal anti-GFP (Abcam ab1218 ...
-
bioRxiv - Bioengineering 2019Quote: ... and cloned into the standard mammalian expression vector pEF1/myc-His B (Thermo Fisher Scientific, Waltham, MA).
-
bioRxiv - Biochemistry 2019Quote: His-tagged SIVsmm Nef constructs were expressed in BL21 (DE3) star cells (Life technologies, Grand Island, NY), and induced with 0.3 mM IPTG at 25 °C overnight ...