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Citations for Thermo Fisher :
2251 - 2300 of 7360 citations for HDGF2 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... sequence coding for the amino acid 319-541 and fused with the N-terminal SARS-CoV-2 spike secretory signal and a C-terminal hexa-histidine tag was transfected into 293F cells cultivated in Freestyle 293 expression media (Thermo Fisher, #12338018) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... plasmids with antibody heavy and light chain genes were used to transfect suspension Expi 293i cells using ExpiFectamine 293 transfection reagents (Thermo Fisher Scientific) as described (20) ...
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bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
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bioRxiv - Immunology 2022Quote: ... Cells grown to a density of 3 million per mL were transfected using the respective plasmids with the ExpiFectamine™ 293 Transfection Kit (ThermoFisher Scientific) and cultivated for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid DNA (1µg/mL) and ExpiFectamineTM 293 reagent were individually diluted with Opti-MEM Reduced Serum Medium (Thermo Fisher, Waltham, MA), incubated 5 min at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: Individual plasmids or combinations of (sub)species-specific gH and gL expression plasmids were transfected into FreeStyle 293-F cells (Thermo Fisher Scientific) using polyethylenimine (PolySciences) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells in a total culture volume of 10 ml were transfected with 10 µg of plasmid DNA using the ExpiFectamineTM 293 Transfection Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: S1PR1-Flag and CD69-StrepII were separately expressed using baculovirus-mediated transduction of mammalian HEK293S GnTI− cells (ATCC CRL-3022) in a medium containing FreeStyle 293 (Gibco Cat# 12338018) supplemented with 2% charcoal-dextran stripped fetal bovine serum (Gibco Cat# 12676029) ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were produced by transient co-transfection of Expi293F cells (maintained in serum-free Expi293 Expression Medium) with heavy- and light-chain constructs using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific), and subsequently purified using Protein G Sepharose 4 Fast Flow (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... After verification by sequencing the constructs were subsequently introduced into Flp-In™ T-REx™ 293 cells (Life Technologies, Carlsbad, CA) as described by Liu et al 52,53.
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bioRxiv - Cell Biology 2023Quote: ... SJ293TS cells were transfected with the transfer vector and the helper plasmids pCAG-kGP1-1R, pCAG-VSVG and pCAG4-RTR2 using PEIpro (Polyplus Transfection, Strasbourg, France) and grown in Freestyle 293 Expression media (Thermo Fisher Scientific) at 37°C with 8% CO2 and shaking at 125 RPM ...
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bioRxiv - Synthetic Biology 2023Quote: ... proteins were transiently expressed in FreeStyle 293-F cells using pSecTag2A plasmids and 293Fectin transfection reagent according to the suppliers’s protocol (Thermo Fisher Scientific; 12347019). 4-6 days after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Expi293F cells were grown at 37℃ with 8% CO2 and DNA transfections were conducted with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatants were harvested three days post transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Expi293F cells were grown at 37℃ with 8% CO2 and DNA transfections were conducted with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatants were harvested four days post-transfection and proteins were purified using HisTrap™ High Performance column (Cytiva) ...
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bioRxiv - Immunology 2023Quote: ... comprising the variable heavy chain and light chains joined with the 15-residue linker was transiently expressed in a secreted form using FreeStyle™ 293-F cells (Thermo Fisher) in FreeStyle™ F17 Expression Medium supplemented with L-glutamine and 1x MEM non-essential amino acids (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... Expi293F cells were diluted to a density of 3 million cells per mL and transfected using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cells were incubated shaking at 130 rpm at 37°C and 8% CO2 ...
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bioRxiv - Immunology 2023Quote: ... The ectodomain of ancestral SARS-CoV-2 spike (GenBank: MN908947.3) was expressed as previously described.10 The protein was purified from FreeStyle 293-F cells (Thermo Fisher Scientific, USA) using affinity chromatography followed by size exclusion chromatography ...
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bioRxiv - Biophysics 2023Quote: ... 1 L of Expi293 cells were grown at 37 °C under a 5% CO2 atmosphere in Gibco® FreeStyleTM 293 Expression Medium (ThermoFisher Scientific) to reach the density of 2 × 106 mL-1 ...
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bioRxiv - Biochemistry 2023Quote: ... Expi293F cells were grown at 37℃ with 8% CO2 and DNA transfections were conducted with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatants were harvested four days post-transfection and proteins were purified using HisTrap™ High Performance column (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... was expressed in Expi293 cell line (3X106 cells/ml, 200 ml in 1 L flask) using ExpiFectamine™ 293 Transfection Kit (Gibco, A14524). After 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ... HEK-293 cells with 80% of confluency were transfected using 250ng/ml of pCMVHA hEZH2 and Lipofectamine 2000 (#11668030, Invitrogen, Thermo Fisher Scientific) for 4 hrs ...
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bioRxiv - Biochemistry 2024Quote: ... Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen, Thermo Fisher Scientific). Flp-In 293 cells were cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... The HA–6XHIS and HA–AviTag constructs for each HA were co–transfected using 293Fectin Transfection Reagent into FreeStyle™ 293–F Cells (ThermoFisher Scientific) at a 2:1 ratio ...
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bioRxiv - Bioengineering 2024Quote: A mixture of 1 μg of antibody-expressing plasmid was transfected into FreeStyle 293 cells using the 293fectin Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions and cultured in FreeStyle 293 Expression Medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) and cultured at a density of 3-5×106 cells/ml in a 37°C incubator with ≥80% relative humidity and 8% CO2 on an orbital shaker at 125×g for 3-4 days ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma human glucagon was measured by the human glucagon ELISA kit (Thermo Scientific, Frederick, MD). Exendin-4 plasma levels were measured using the exendin-4 EIA kit (Phoenix pharmaceuticals ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 50 μL fresh FACS buffer and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Immunology 2022Quote: ... Total IgG in human sera was measured using a commercial human IgG ELISA kit (Fisher Scientific) according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... All human CD8+ T cells were activated with anti-human CD3/CD28 dynabeads (11131D, Thermo Scientific) at a 1:1 cell-to-bead ratio and cultured in complete RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of human CCDC22 and CCDC93 and full-length human DENND10 were ordered as GeneStrings (ThermoFisher) with the gene codon optimized for E ...
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bioRxiv - Cell Biology 2023Quote: ... Purified human neutrophils and T cells were cultured in Human Plasma-Like Medium (HPLM; ThermoFisher Scientific) with 5% heat inactivated FBS supplemented ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were thawed and activated with Human T-Expander CD3/CD28 Dynabeads (Gibco) at a 3:1 bead:cell ratio in complete medium (RPMI 1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: ... Human Jurkat T cells and human THP-1 monocytes were cultured in RPMI 1640 medium (ThermoFisher), supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: Fresh human BAL cells and frozen human PBMCs were stained with Aqua live/dead dye (Invitrogen) for 20min at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Neuroscience 2023Quote: ... Secreted Aβ42 peptides were quantified using the Human Amyloid β1-42 human ELISA kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... human melanocyte growth supplement (HMGS, Gibco), gentamicin (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Pool A (human) (Thermo Fisher Scientific) using the TaqMan® microRNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... human skeletal myoblasts (Thermo Fisher Scientific), and mouse C3H muscle myoblasts (C2C12 cells ...
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bioRxiv - Cancer Biology 2019Quote: ... human keratinocyte growth supplement (ThermoFisher Scientific), and penicillin-streptomycin ...
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bioRxiv - Cell Biology 2019Quote: Episomal human iPSCs30,31 (Thermo Fisher Scientific) were maintained as a monolayer on Matrigel (Corning Life Sciences ...
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bioRxiv - Cancer Biology 2020Quote: ... with human melanocyte growth supplements (Gibco). Cell lines were incubated at 37 °C in 5% CO2.
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bioRxiv - Molecular Biology 2019Quote: ... Human colon total RNA (Thermofisher Scientific) was aliquoted at 13 µg/mL and stored at −20°C
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bioRxiv - Cancer Biology 2021Quote: Human melanocytes were trypsinized (Gibco, 25300062), quenched ...
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bioRxiv - Systems Biology 2021Quote: ... The human 62-plex (eBiosciences/Affymetrix) was utilized with the modifications described below ...
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bioRxiv - Bioengineering 2020Quote: ... PE-conjugated anti-human CXCR4 (Invitrogen), FITC-conjugated anti-human ACE2 (R&D Systems) ...