Labshake search
Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 6 hydroxy 2 methylquinazolin 4 3H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
-
bioRxiv - Immunology 2022Quote: ... serum Igs were purified from H10ssF-immunized mice (combined 2-4 weeks after the third immunization) by using protein A/G (Pierce Thermo Scientific). Seven mg of purified heperimmune Igs or control Igs or 100 μg (5 mg kg-1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times (200 rpm, 5 min at 4°C) in eBioscience™ Permeabilization buffer (250 µl/well) (Invitrogen) and resuspended in eBioscience™ Fixation/Permeabilization solution (Invitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... Jurkats were treated with 150mJ of UV energy and incubated for 2-4 hours at 37°C in complete media consisting of RPMI 1640 Media (Gibco, 11875101), 10% FBS (R&D Systems ...
-
bioRxiv - Physiology 2022Quote: ... An aliquot of the polar phase was collected and vacuum-dried at 4°C and subsequently derivatized with 2% (w/v) methoxyamine hydrochloride (Thermo Scientific) in pyridine for 60 min following by 30 min sialyation N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were fixed for 15 min at 37°C in 4% paraformaldehyde and incubated with 2 μM Bodipy 493/503 (Bodipy 493, Molecular Probes) and 1 μg/ml of DAPI as published (9) ...
-
bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
-
bioRxiv - Cell Biology 2022Quote: ... The remaining 50 μL were incubated at 4 °C for 2 h with 40 μg of HisPur Ni–NTA magnetic beads (Thermo Scientific) pre-equilibrated in binding buffer ...
-
bioRxiv - Immunology 2021Quote: ... and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final: 10% FBS, 1x GlutaMAX™, 50µM 2-mercaptoethanol Thermo Scientific™ ...
-
bioRxiv - Biochemistry 2019Quote: ... For MS analysis proteins were run for 1-2 cm on a 4-12% PAGE (NuPAGE Bis-Tris Precast Gel, Life Technologies) and stained with silver (SilverQuest Silver Stain ...
-
bioRxiv - Microbiology 2019Quote: ... pneumoniae TIGR4 was detected using 1:2 optimized rabbit type-4 antiserum and 1:100 anti-Rabbit-Alexa-Fluor-488 (Thermo Fisher). Binding of human FH (Merck Millipore ...
-
bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
-
bioRxiv - Bioengineering 2021Quote: ... Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher). Cells were incubated with 100 nM TO-1 in HBSS starting 30 minutes prior to imaging and for the duration of the imaging period ...
-
bioRxiv - Bioengineering 2020Quote: ... the media was removed and the gels were washed once with PBS prior to the addition of 500uL of 1X PBS with Calcein AM (2 µM) and Ethidium Homodimer-1(4 µM) (Invitrogen, L3224). After 45 minutes of incubating the stains ...
-
Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: HeLa cell pellets (∼2×107) or MCF7 cell pellets (∼4×107) were lysed with 3 mL Mammalian Protein Extraction Reagent (Thermo Scientific), supplemented with 2X HALT protease inhibitor (Thermo Scientific) ...
-
bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
-
bioRxiv - Microbiology 2020Quote: ... cells were resuspended in 2 mL of 4 % FA in PBS (made from 16% Formaldehyde solution, Methanol-free from Thermo Scientific). Falcons were placed on a rotor for constant agitation for 45 min at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... The transfection mixture was prepared by mixing 2 µg plasmid DNA and 4 µl Cellfectin-II reagent (Invitrogen, ThermoFisher Scientific, USA) to a final volume of 200 µl serum-free media ...
-
bioRxiv - Molecular Biology 2020Quote: ... The transfection mixture was prepared by mixing 2 µg plasmid DNA and 4 µl Cellfectin-II reagent (Invitrogen, ThermoFisher Scientific, USA) to a final volume of 200 µl serum-free media ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were extracted using lysis buffer (8 M urea, 2 M thiourea, 4% CHAPS, 40mM DTT) supplemented with Halt protease inhibitor complete cocktail (Thermo Fisher) by bead beating (50 Hz for 3 five-minute cycles ...
-
bioRxiv - Cell Biology 2022Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
-
bioRxiv - Biochemistry 2022Quote: ... pH 7.4/50 mM KOAc/2 mM EDTA, pH 8/1 mM DTT/1 mM PMSF/1x Phosphatase inhibitor cocktail, Thermo Scientific), 0.3 g of glass beads (acid washed ...
-
bioRxiv - Physiology 2022Quote: ... Separation was performed with a Dionex Ionpac AS11-HC column (2 mm x 250 mm, 4 μm particle size, Thermo Fisher) at 30°C ...
-
bioRxiv - Physiology 2022Quote: ... The corpus was incubated in 50% betaisodona/PBS (Mundipharma GmbH) for 30 min at 4°C before being washed in different solutions for 2 min each: PBS (ThermoFisher Scientific), 0.1% octenidin in ddH2O (Serva Electrophoresis) ...
-
bioRxiv - Genomics 2022Quote: ... resuspended in ice cold PBS+BE (1 mg/mL BSA + 2 mM EDTA) and incubated for an hour at +4°C with Dynabeads Biotin Binder (Thermo Fisher). Cells were repeatedly washed with ice-cold sterile PBS while on magnet ...
-
bioRxiv - Plant Biology 2021Quote: ... two complementary oligonucleotides with 4-bp overhangs (Supplemental Table 2) were annealed and inserted by a Golden Gate reaction with BpiI (Thermo Scientific) and T4 DNA ligase (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pre-cleared chromatin (150 μg) was incubated with 4 μl (2 μg) of monoclonal mouse anti-V5 antibody (Invitrogen R960-25) for 1 hr at 4C with rocking ...
-
bioRxiv - Neuroscience 2020Quote: ... at 4 ng/μL and then pulled-down for 2 h using protein G-coupled dynabeads (50 μL, Thermo Fisher Scientific). After washing 3 times with 0.3 M NaCl solution ...
-
bioRxiv - Plant Biology 2021Quote: ... The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20) replacing the buffer every 2 hours and incubated overnight at 4°C with the appropriate secondary antibodies (ThermoFisher Scientific), anti-Rabbit-Alexa 488 (RA3) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Captured cells were stained with 2 μM green-fluorescent calcein-AM and 4 μM red-fluorescent ethidium homodimer-1 (Molecular Probes) and visualized under an EVOS FL cell imaging station (Life Technologies ...
-
bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
-
bioRxiv - Genetics 2022Quote: ... Ly6Chi monocytes were labeled by intraperitoneal injection of 4 mg/mL of Edu (5-ethynyl-2’-deoxyuridine) from Life technologies (NY), and mice were euthanized after 5 days to assess baseline recruitment or 21 days to assess for retention ...
-
bioRxiv - Genomics 2019Quote: Single cells were stained with 4 μM Ethidium homodimer-1 and 2 μM Calcium AM (ThermoFisher LIVE/DEAD Viability Kit L3224) in the dark at room temperature for 20 min ...
-
bioRxiv - Physiology 2019Quote: ... using 4 µL of 5x VILO reaction buffer and 2 µL of 10x SuperScript Enzyme Mix reverse transcriptase (Thermo Fisher Scientific) at 25°C for 10 min ...
-
bioRxiv - Systems Biology 2020Quote: Similar to our recent report,16 1 mg nuclear protein extracted from Raw 264.7 macrophage cells was incubated overnight at 4°C with 2 nmole UNC0965 pre-coupled to 50 μl neutravidin-agarose (Thermo Fisher), and washed three times with 1 ml lysis buffer to remove non-specific bound proteins ...
-
bioRxiv - Genetics 2021Quote: ... The lysate was incubated for 2 hours in a rotating wheel at 4°C with Dynabeads Protein A (#10002D Dynabeads Protein A, ThermoFisher Scientific) as a preclearing step ...
-
bioRxiv - Developmental Biology 2020Quote: ... while the remainder was immunoprecipitated for 2 hr at 4°C in the following antibodies: rabbit anti-GFP (Invitrogen, A-11122), rabbit anti-Cen ...
-
bioRxiv - Molecular Biology 2021Quote: ... the cells were stained between 2 and 4 hours after extraction using a LIVE/DEAD® Cell Imaging Kit 488/570 (Invitrogen), and following the manufacturer’s protocol ...
-
bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... and either pINDUCER-20-IRS2 or pHAGE-FLAG-HA-NTAP-IRS2 in a 4:2:1 DNA ratio using Lipofectamine 3000 (Invitrogen, L3000001) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Thermo Scientific, Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2020Quote: ... boiled in reducing sample buffer for 5 min and resolved on 4–12% Bis-Tris Bolt gels and transferred using an iBlot 2 (Thermo Fisher). Blots were blocked in 2.5% milk in 1% TBS-Tween before staining with antibodies (Table S5) ...
-
bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
-
bioRxiv - Genetics 2021Quote: ... The lysate was cleared by centrifugation and incubated for 2 hours at 4°C with Tag specific magnetic beads (Dynabeads™ M-280 Streptavidin/Invitrogen for immunoprecipitation of Avitag-ZFP462 and Avitag-GLP ...
-
bioRxiv - Synthetic Biology 2021Quote: ... cells were dissociated into single cells with TrypLETM for 4-5 minutes at 37 C and seeded into 2 % Geltrex coated 96- or 12-well plates (Fisher Scientific) in Essential 8TM Medium (Gibco ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 days at 4°C with rabbit anti-GFP antibody (1:2,000; Thermo Fisher Scientific, USA). The sections were then incubated with biotinylated donkey anti-rabbit IgG antibody (1:1,000 ...
-
bioRxiv - Immunology 2021Quote: ... samples were incubated at 4°C covered from light for 30 minutes with 2 μl/ml Fixable Viability Dye eFluor506 (Thermo Fisher) while in PBS and then resuspended in rat anti-mouse CD16/32 (Fc block ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were seeded at a density between 2 × 104 and 4 × 104 cells per well in Nunc Lab-Tek II CC2 chamber slides (Thermo Scientific) based on their proliferation rate and left to establish for 48hrs at 37°C ...
-
bioRxiv - Genomics 2019Quote: ... 2 and 4 h post dosing on day 3 and collected on ice in microcentrifuge tubes containing K2-EDTA (Fisher Scientific). Within 30 min after collection ...