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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for 6 hydroxy 2 methylquinazolin 4 3H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Cells were loaded at 37°C for 60 minutes with 4 μM Fura-2-AM in the presence of 1X Power Load permeabilizing reagent (Thermo Fisher). Injection of AngII (10-8M) ...
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bioRxiv - Genomics 2024Quote: ... was prepared fresh with the 3’aminated 5’ caged oligo root carrying a terminal Cy3 dye (2 μM BL003 for the single-cycle experiment and 2 μM BL728 for the 4-cycle experiment) and a bifunctional crosslinker BS(5)PEG (Thermo Scientific) (5 μM in dimethylformamide) ...
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bioRxiv - Microbiology 2024Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed once with PBS and a mixture of 1:1:2 (w/w) of pcDNA5-αSyn (4 μg): pOG44 Flp-recombinase expression vector (pOG44, Invitrogen #V600520):PEI in 2 mL of DMEM was added for 8 h ...
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bioRxiv - Neuroscience 2024Quote: The pipette solution (EGTA omitted) was supplemented with 100 μM calcium-sensitive dye Fluo-4 or Fura-2 (Thermo Fisher Scientific) and 50 μM calcium-insensitive dye Alexa Fluor 568 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining 45 μL were added to 2 μL of settled anti-Flag magnetic beads (4 μL of 50% slurry) (Thermo Fisher) that had been equilibrated in Wash Buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... all exosome samples were diluted with one volume of Exosome Resuspension Buffer and one volume of 2X Denaturing Solution (both from Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), followed by protein precipitation with dichloromethane (Merck ...
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bioRxiv - Genomics 2024Quote: ... The quality of the extracted RNA was checked using NanoDrop™ One/OneC Microvolume UV- Vis Spectrophotometer (Thermo Scientific™ Cat. No. ND-ONE-W) and gel electrophoresis ...
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bioRxiv - Physiology 2024Quote: ... RNA concentration and purity were assessed by UV spectroscopy at ODs of 260 and 280 nm using a Nanodrop (ThermoFisher One/One UV spectrophotometer, USA). RNA integrity numbers (RIN ...
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bioRxiv - Cancer Biology 2019Quote: ... 300,000/6-well) and IDC (5,000/96-well; 100,000/6-well) cells were seeded in regular (Thermo Fisher Scientific) or ULA (Corning Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were forward transfected in 6-well dishes by using 6 μl of FuGENE® HD (PRE2311, Fisher Scientific) with 3 μg of sgRNA ...
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bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Cell Biology 2020Quote: Cells in the 6-well plates were transfected with 2μg plasmid of AMPKα or OGG1 via 6 μl Lipofectamine 2000 (Invitrogen) for 6 hours following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The Cas9-sgRNA-containing plasmid and donor vector were co-transfected at a molar ratio of 1:6 into XX mESCs cells on a 6-well plate using Lipofectamine 2000 according to the manufacturer’s protocol (ThermoFisher). Transfected cells were passaged at different densities into three Petri dishes with feeders ...
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bioRxiv - Biochemistry 2021Quote: ... 6% native PAGE gels were prepared by first making a gel solution containing 6% acrylamide (29:1) (Fisher Scientific).15 This solution was then degassed under vacuum and mixing ...
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bioRxiv - Molecular Biology 2022Quote: ... One-step RT-PCR using a SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase (Thermo Fisher Scientific, Waltham, MA, USA) was performed using a combination of primers [Mar-CPL-F and MarCP2R (876 bp)] designed specifically for minor CP amplification.
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bioRxiv - Genomics 2024Quote: ... The quality of the extracted RNA was validated with the NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific™ Cat. No. ND-ONE-W) and 1% agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2020Quote: ... One ml of HisPur™ Ni-NTA Superflow Agarose (ThermoFisher Scientific) slurry was added to the extract and incubated for 15 min at 4°C with shaking ...
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bioRxiv - Plant Biology 2020Quote: ... one tablet/50 mL EDTA free Pierce protease inhibitor (Thermo Fisher)) at 2:1 ratio (2 mL buffer ...
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bioRxiv - Microbiology 2019Quote: ... one or two million cells suspended in CO2-independent medium (Gibco) supplemented with 10% FBS were mixed with VSV-G-pseudotyped virus stocks and shaken at 220 rpm for 2 h at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: One liter of molecular grade water (Fisher Scientific, Cat # BP2819-1) was filtered before the experimental samples and analyzed as a system control to ensure the filtration system was sterile ...
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bioRxiv - Molecular Biology 2020Quote: ... and quality was assessed with NanoDrop™ One (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... One hour prior to fixation 10 µM ethylene-deoxyuridine (EdU, Thermofisher) was added to the media ...
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bioRxiv - Molecular Biology 2021Quote: ... the concentration determined with a NanoDrop™ One spectrophotometer (ThermoFisher Scientific), and stored at −80°C until further use.
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bioRxiv - Immunology 2021Quote: ... and gene expression was examined with Step One Plus (Applied Biosystems) using TaqMan® Real Time PCR Assay ...
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bioRxiv - Genomics 2020Quote: ... and transformed into One Shot Stbl3 Chemically Competent E.coli (Life Technologies). A correct cloning product was identified by control restriction enzyme digestion using AarI (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... Emulsion PCR was performed using the One Touch system (Life Technologies). Beads were prepared using the One Touch 2 and Template Kit v2 ...
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bioRxiv - Genomics 2019Quote: ... and the RNA was quantified using a NanoDrop One spectrophotometer (Thermofisher).
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bioRxiv - Molecular Biology 2019Quote: ... One hundred μL of Dynal protein G beads (Thermo Fisher Scientific) were washed three times in PLB and were resuspended in 1 mL of PLB ...
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bioRxiv - Immunology 2019Quote: ... was performed in triplicate using a Step One Plus (Applied Biosystems) real time detection system ...
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bioRxiv - Microbiology 2021Quote: ... RNA concentrations were determined on NanoDrop One microspectrophotometer (ThermoFisher Scientific, USA), and RNA purity was assessed by OD260/OD280 and OD260/OD230 ratios.
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bioRxiv - Cancer Biology 2019Quote: ... All-in-One™ First-Strand cDNA Synthesis Kits (Thermo Scientific) were used to reverse transcript the RNA sample (1 μg ...
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bioRxiv - Biochemistry 2020Quote: ... One Shot® TOP10 Chemically Competent Escherichia coli (Thermo Fisher Scientific) cells were transformed to amplify the mutated genes ...
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bioRxiv - Cell Biology 2021Quote: ... One wash included Hoechst 33342 (1:10,000; H-3570; Molecular Probes) to stain nuclei ...
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bioRxiv - Physiology 2021Quote: ... and analyzed with SuperScript III Platinum SYBR Green One-Step (Invitrogen). As previously described41,42 ...
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bioRxiv - Physiology 2021Quote: ... and quantity was determined using a NanoDrop One spectrophotometer (Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... One testis was cut half and homogenized with Trizol (Thermo Fisher) for RNA isolation and snap-frozen for protein extraction ...
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bioRxiv - Plant Biology 2021Quote: ... in a Step-One Plus Real-Time PCR system (Applied Biosystems). The expression levels were normalized against a ubiquitin reference gene ...
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bioRxiv - Microbiology 2020Quote: ... We transformed One Shot™ BL21(DE3) Chemically Competent (Thermo Scientific) E ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by addition of one volume of 70% ethanol (Fisher Scientific) and mixed again by pipetting ...
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bioRxiv - Cell Biology 2020Quote: ... One microgram of RNA was reverse transcribed using SuperScript III (Invitrogen) and quantitative PCR were assayed using Mus musculus primers against Vegfa (Mm00437306_m1 ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA from one worm was extracted with TRIzol™ (Invitrogen) using the standard protocol ...
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bioRxiv - Pathology 2022Quote: hPCLS were infected for one hour in 100 µL MEM (Gibco) in 24-well plates at 37°C with human rhinovirus 16 (Virapur ...
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bioRxiv - Biophysics 2022Quote: ... cDNA concentration was checked via NanoDrop (Thermo Scientific, ND-ONE-W), and then aliquoted and stored at −20°C until used for PCR ...
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bioRxiv - Biochemistry 2022Quote: ... Final protein concentration was measured using NanoDrop One (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... which was grown in One Shot PIR1 cells (Thermo Fisher Scientific). Plasmids were purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNA was quantified in a Nanodrop One spectrophotometer (Thermo Fisher Scientific) and by fluorometric quantitation using a PicoGreen device (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was determined with NanoDrop One spectrophotometer (Thermo Fisher Scientific).