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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged hAgo2 was purified from Sf9 cells using a baculovirus system (Thermo Fisher). Cells were lysed in Lysis Buffer (300mM NaCl ...
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bioRxiv - Microbiology 2022Quote: Pull-down assays were carried out using Dynabeads His-tag Isolation and Pulldown (Invitrogen). The 6His-tagged soluble pilin domains were used as bait ...
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bioRxiv - Biochemistry 2022Quote: ... and cultured in house) were grown to 70% confluency in DMEM-Hi glucose (Gibco) supplemented with 10% heat-inactivated foetal bovine serum (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were digested and ligated into pcDNA3.1 myc-His B expression vectors (Invitrogen), with a 3xFlag epitope tag also fused to the N-terminus of Ptchd1 ...
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bioRxiv - Biochemistry 2022Quote: ... redundant anti-His antibody was washed away and 15 μL chemiluminescence substrate (Thermo Scientific) was added onto each well to generate chemiluminescence signal ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco™ Cat. #10438026) and 1% antibiotic/antimycotic (Gibco™) ...
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bioRxiv - Plant Biology 2022Quote: ... and purified by Dynabeads™ His-Tag Isolation and Pulldown (ThermoFisher, Catalog number 10103D). DA1 swapped protein ubiquitylation reactions used E1 ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 30 minutes on ice with His-Pur Ni-NTA resin (Thermo Scientific), packed to a height of approximately 5 cm in polypropylene columns ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and His-p115 were affinity purified using Ni-NTA HisPur beads (Thermo Fisher Scientific) from crude E ...
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bioRxiv - Microbiology 2023Quote: ... A standard curve of 10-fold dilutions of plasmid pAc5.1-V5-His-A (Invitrogen) containing the DCV ORF-2 sequence was used to convert Ct values to genome copy numbers.
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bioRxiv - Evolutionary Biology 2022Quote: ... and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (Thermo Fisher Scientific #MA1-21315) diluted in 5% milk in PBS-T (1:500) ...
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bioRxiv - Molecular Biology 2022Quote: ... Junction PCR was performed with Extensor Hi-Fidelity PCR Master Mix (Thermo Fisher Scientific) in a 20 μL reaction using 500 nM of target-specific primers and the following cycling conditions ...
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bioRxiv - Immunology 2023Quote: ... M4 cells are maintained in DMEM/F12 media with 5% HI HS (Gibco #26050088).
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bioRxiv - Neuroscience 2023Quote: ... and subcloned into the pcDNA™3.1/myc-His(−)A vector (Thermo Fisher Scientific). We accidentally obtained the pcDNA™3.1/myc-His(−)A-(G4C2)9 vector at this step ...
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bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... HI samples were incubated with 1 mL of IgG Fc Capture Select (Thermo Fisher) resin at 4C for 20 to 48 h to bind IgG ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then heated with Novex Hi-Density TBE sample buffer (Invitrogen LC6678) at 95°C for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS) (10082147, Thermo Fisher Scientific) and 1% penicillin/streptomycin 1000 U/mL (Pen/Strep ...
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bioRxiv - Developmental Biology 2024Quote: ... wild type Cic and mutant variants were subcloned into pMT-V5-His vector (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Cancer Biology 2024Quote: Isolated human PBMCs (ATCC PCS-800-011) were activated with human monoclonal antibodies against human CD3 (OKT3, Life Technologies, 16-0037) coated onto a flask and 2 ug/ml CD28 (CD28.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Thermofisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Thermofisher Scientific, Carlsbad, CA) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Epidermal growth factor receptor (EGFR) protein expression levels on the surface of Gli36-derived EVs were quantified using an EGFR Human ELISA kit (ThermoFisher Scientific, Waltham, MA). EVs were spiked in healthy donor serum at different concentrations ranging from 0 to 1.0E11 particles/mL while maintaining the serum-derived EV concentration at 1.0E9 particles/mL ...
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Neuroscience 2024Quote: ... were determined by matching the UniProt human protein database (release 2023_01) with the acquired fragmentation pattern using Sequest (Thermo Fisher Scientific, Waltham, MA) (Eng et al. ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... total nucleic acid was extracted from tissues using the MELT™ Total Nucleic Acid Isolation System (Ambion, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... We then proceeded with a standard hot phenol-acid extraction (Acid Phenol:Chloroform mix 125:24:1, Invitrogen, AM9722). The samples were resuspended in nuclease-free water and quantified on a Spectrophotometer ...
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bioRxiv - Neuroscience 2021Quote: ... 0.8 mM KH2PO4, 20 mM NaHCO3, 1.3 mM L-glutamine, 0.2 mM ascorbic acid, MEM amino acids solution [Gibco; Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... omitting all amino acids without the addition of non-essential amino acids and substitution with dialyzed FBS (Invitrogen). Doxycycline treatment of cells stably expressing ER-phagy probe was performed as previously described7.
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bioRxiv - Pathology 2023Quote: ... Dilutions of SCFAs standards (Acetic acid:A6283, Sigma-Aldrich; Propionic acid: 81,910, Sigma-Aldrich; Butyric acid:108,111,000, Acros Organics) were prepared in saline solution at concentrations ranging from 87 to 0.087 mM for acetic acid ...
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bioRxiv - Cell Biology 2019Quote: ... primary and secondary antibodies were incubated in Ca2+-containing annexin V binding buffer (Life Technologies) to preserve annexin V binding ...
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bioRxiv - Microbiology 2019Quote: ... The binding was detected with Alexa Fluor-647-labeled streptavidin (Molecular Probes, 1 μg/ml). All steps were carried out at ambient temperature ...
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bioRxiv - Microbiology 2019Quote: ... JAR4 binding was detected using FITC-conjugated rat anti-mouse IgG (H+L) (Thermo Fisher) at a 1:50 dilution for an incubation period of 1 h ...
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bioRxiv - Microbiology 2021Quote: ... HumAbs were purified using the Gentle Ag/Ab binding and Elution Buffer kit (Thermo Scientific). HumAbs were concentrated using Millipore amicon ultra centrifugal filter tubes (30K MWCO ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was retained in a 2 ml low binding microcentrifuge tube (Fisher Scientific, UK). The pellet was resuspended in 500 µl acidified methanol using a sterile plastic pestle ...
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bioRxiv - Systems Biology 2021Quote: ... The reactions were performed using unlabeled primers and SYBR Green DNA binding dye (Applied Biosystems) with a volume of 12 μL and the final primer concentrations of 0.4 μM ...
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bioRxiv - Microbiology 2020Quote: ... Specific antibody binding was detected by peroxydase-labelled Goat anti-Hamster IgG (H+L) (Invitrogen) and peroxydase-labelled Goat anti-Ferret IgG (H+L ...
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bioRxiv - Bioengineering 2022Quote: ELISA binding assays were performed using 96 well half-area plates (Fisher Scientific, Costar 3690) coated with 0.5 μg/mL of S protein and incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: 500-20,000 sorted cells were lysed with 100 μl of lysis/binding buffer (Life Technologies), snap-frozen on dry ice and stored at −80°C until further use ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary Ab binding was detected by AlexaFluor-conjugated econdary Abs (Invitrogen, 1:400-1:1000). Nuclei were stained with DAPI (Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: DNA binding assay was performed using the LightShift Chemiluminescent EMSA Kit (Thermo Scientific Cat # 20148) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... AlexaFluor 594 was used to visualize antibody-binding for Pax7 (1:100, TSA kit, Invitrogen). Sections were mounted and nuclei were stained with Vectashield with DAPI (H-1200 ...