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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 1% non-essential amino acids (Gibco) 0.1% β-mercaptoethanol and 1% penicillin-streptomycin (29) ...
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bioRxiv - Genomics 2020Quote: ... SEMA3C protein levels were checked by western blot as described above using rabbit anti-human SEMA3C antibody (1:1000, Thermo Fisher Scientific). Beta-actin (housekeeping gene ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Raw MS Data were searched against the human SwissProt protein database (Version November 2020) with Proteome Discoverer 2.4 (ThermoFisher Scientific, Bremen, Germany) using the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... and cultured in RPMI medium supplemented with 10% FBS and differentiated for 7 days with 20 ng/mL recombinant human Macrophage-Colony Stimulating Factor protein (M-CSF) (Thermo Fisher Scientific), and 100U/L of penicillin-streptomycin solution (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human mAbs and CaptureSelect CH1-XL MiniChrom columns (Thermo Fisher Scientific) for Fab fragments ...
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2022Quote: ... All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) and purified as previously described (4 ...
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bioRxiv - Neuroscience 2022Quote: ... similarly as described previously.[60] MS data were analyzed using an in-house maintained human protein sequence databases using SEQUESTTM and Proteome DiscovererTM (Thermo Fisher Scientific). The mass tolerances of a fragment ion and a parent ion were set as 0.5 Da and 10 ppm ...
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Immunology 2023Quote: Plasmids coding for the soluble S ectodomain proteins as well as the RBD of human coronaviruses were transfected into Freestyle293F cells (Thermo Fisher R79007). Per 1 L of Freestyle 293F cells ...
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Phosphorylation was induced by incubating 1 µg of recombinant human polo-like kinase 3 (PLK3) protein (Thermo Fisher Scientific, MA, USA; #PR7316B) with 50 µL of mouse WT α-synuclein in kinase reaction buffer for 8 hrs at 30°C ...
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bioRxiv - Biochemistry 2024Quote: MS/MS searches were carried out using SEQUEST search algorithms against a Uniprot database for humans (supplemented with exogenous sequence for anticipated protein constructs) using Proteome Discoverer (Version 3.0, Thermofisher Scientific, Bremen, Germany). Oxidation of methionine and N-terminal protein acetylation were included as dynamic modifications and carbamidomethylation of cysteine was set as a static modification ...
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bioRxiv - Cell Biology 2019Quote: ... cells were first blocked for non-specific binding using 1% NF BSA from Ambion for 30 minutes at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed and free binding sites were then blocked with Superblock (Thermofisher Scientific) at room temperature for 30 min and then incubated with serial dilutions (200- to 12800-fold for serum ...
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bioRxiv - Immunology 2022Quote: ... and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Immunology 2021Quote: ... HRP binding was determined based on reaction with TMB substrate (34028, Thermo Fisher Scientific) and was stopped by addition of 2 M sulfuric acid (BCH7500-1 ...
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bioRxiv - Cell Biology 2019Quote: ... Antibody binding was detected using ECL Super Signal West Dura Extended Duration Substrate (ThermoFisher) and exposure to maxAgfa CL-XPosurelight films (ThermoFisher).
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bioRxiv - Bioengineering 2021Quote: ... with the staining solution containing 2 drops/ml F-actin binding ActinGreen (ThermoFisher, USA) and 1.25 μg/ml 4’ ...
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bioRxiv - Bioengineering 2020Quote: ... Binding buffer was prepared with 100 mg/mL of yeast tRNA (Thermo Fisher, AM7119) and 1 mg/mL of bovine serum albumin (RPI ...
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bioRxiv - Biochemistry 2021Quote: ... The mAbs were tested for binding using MaxiSorp 96-well plates (Nalgene Nunc International) coated at 2 μg/ml with wt gp120 or gp120 V region deletion mutants in PBS at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 125 µL of MagMAX Cell Free DNA Lysis/Binding Solution (Thermo Fisher cat#AM8500) and 5 µL of Dynabeads MyOne Silane magnetic beads (Thermo Fisher cat#37002D ...
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bioRxiv - Immunology 2019Quote: ... Non-specific binding was blocked by incubating in 5% goat serum (Thermo Fisher Scientific) in PBS for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... nodule sections were stained with the DNA-binding fluorescent dye SYTO13 (Invitrogen, Eugene, Oregon). The preparation of nodule sections was carried out as described earlier (Domonkos et al. ...
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bioRxiv - Neuroscience 2019Quote: ... non-specific binding sites were blocked with PBS containing 5% BSA (Fisher Scientific, AAJ1085718) and 0.3 M glycine ...
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bioRxiv - Immunology 2019Quote: ... Streptavidin binding peptide (SFB)-MCM10 stable cell lines were grown in DMEM (Gibco 11995) supplemented with 10% FBS (Sigma F4135) ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were re-suspended in 200 µl lysis/binding buffer (Invitrogen, Darmstadt, Germany), frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies binding was performed using the corresponding HRP-conjugated secondary (1:10000, Invitrogen) for 1 h in 1x TBST at rtp ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were collected directly into 40 µl of lysis/binding buffer (Life Technologies) and mRNA was isolated using 15 μl dynabeads oligo (dT ...
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bioRxiv - Cancer Biology 2019Quote: Tumours were dissected in PBS and incubated in Annexin V binding buffer (Fisher Scientific) containing 5% of Alexa Fluor (488nm or 568nm ...
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bioRxiv - Microbiology 2021Quote: ... and coated onto 96-well plates (MaxiSorp - ThermoFisher or High binding - Greiner Bio-one) using 10nM (10 pmols/mL) ...
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bioRxiv - Biochemistry 2019Quote: Binding affinity of guanine nucleotides BODIPY-FL-GTPgammaS or BODIPY-FL-GDP (Molecular Probes) were determined using fluorescence polarization measured by an EnVison 2102 Multilabel Plate Reader (Perkin Elmer).
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bioRxiv - Biochemistry 2020Quote: ... Nanobody binding was detected using Myc-tag specific antibody (2 μg/ml – Life Technologies) and then anti-mouse-Alexa Fluor 700 (1.3 μg/ml – Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Nonspecific antibody binding was blocked with a 10% Goat Serum (Thermo Fisher (Waltham, MA)) overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: ... pan-cytokeratin (nanoString, AE1/AE3, #121301310) and a DNA binding dye (ThermoFisher, Syto13, S7575) were applied to the slides for 1 hour at room temperature.
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bioRxiv - Cancer Biology 2021Quote: ... counted and 1×106 cells resuspended into 1 annexin-binding buffer (Thermo Fisher Scientific). 2ul Alexa Flour 488 Annexin V (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... EqPD-L1-Ig binding was detected using horseradish peroxidase-conjugated Neutravidin (Thermo Fisher Scientific) and TMB One Component Substrate (Bethyl Laboratories ...
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bioRxiv - Immunology 2020Quote: ... GC-derived B cells were sorted in bulk into lysis/binding buffer (Life technologies)
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Antibody binding was detected by fluorophore-conjugated highly cross-adsorbed secondary antibodies (ThermoFisher Scientific), and nuclei were stained with Hoechst 33342 (ThermoFisher Scientific) ...
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A stable reference human transcriptome and proteome as a standard for reproducible omics experimentsbioRxiv - Cell Biology 2022Quote: ... The peptides were collected into low-binding collection tube (Thermo Scientific™, 88379, USA) by centrifugation at 12000 xg for 20 min ...
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bioRxiv - Neuroscience 2022Quote: ... Solution was supplemented with 2 mM of sodium-binding benzofuran isophthalate (SBFI, Molecular Probes).
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bioRxiv - Neuroscience 2023Quote: ... Unspecific binding sites were blocked by DPBS (w/o Mg2+ and Cl2+, #12559069, Gibco) containing 2 % bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2024Quote: Co2+ binding experiments were performed on an Ultrospec 8000 UV-Vis spectrophotometer (Thermo Fisher) for 100 µM Z7 peptide samples in 10 mM Tris pH 7.0 ...
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bioRxiv - Microbiology 2024Quote: ... supernatant (approximately 450 µL) was mixed with 600 µL of the binding buffer (Invitrogen) by pipetting up and down ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was collected in 1.5mL low binding tubes (Thermo Fisher, Waltham, USA). For protein quantification ...
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bioRxiv - Biochemistry 2023Quote: ... The X15 library was depleted for bead-binding phage by incubation with Dynabeads (Thermofisher) before panning ...
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bioRxiv - Molecular Biology 2023Quote: ... Binding biotinylated RNA/DNA to Dynabeads® MyOne™ Streptavidin T1 magnetic beads (ThermoFisher). The RNA/DNA binding reaction mixture included ...
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bioRxiv - Neuroscience 2024Quote: ... IBA-1 (ionized calcium-binding adapter molecule 1; antibody PA5-21274, Invitrogen; 1:150) and astrocytes (anti-S100 beta antibody ...