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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
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bioRxiv - Microbiology 2021Quote: ... and incubated at 30 °C for 48 h in Bioscreen C (ThermoFisher), where the OD600 was measured at 1 h intervals ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-c-myc beads (Pierce Anti-c-Myc Agarose, Thermo Scientific), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... anti-c-myc beads (Pierce Anti-c-Myc magnetic beads, Thermo Scientific), and anti-HA beads (EZ view Red Anti-HA Affinity Gel ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were centrifuged at 12,000 × g for 10 min at 4°C and the supernatant was isolated and quantified using a Pierce BCA protein assay kit (Thermo Fisher Scientific, MA, US). Loading buffer (4× ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and concentrations of the soluble fraction were determined by using the Pierce™ BCA protein assay (Thermo Fisher Scientific, Waltham, MA, USA). 20 μg protein samples were analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Bioengineering 2020Quote: ... the bacteria pellet was thawed on a water bath at 37 °C and lysed with B-PER™ complete bacteria protein extraction reagent (ThermoFisher Scientific, MA, USA). The released nanobody was purified via standard immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Physiology 2021Quote: ... The supernatant was collected by centrifugation at 12,000×g for 10 min at 4°C and the protein concentration was determined using a microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). Equal amounts of extracted protein (30 μg per lane ...
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bioRxiv - Immunology 2022Quote: ... The transfected cells were cultured at 37°C for 24 h and then collected and lysed using protein lysis buffer (Thermo Fisher Scientific, Cat #: 87787). Aliquots of lysate (20 μg protein ...
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bioRxiv - Cancer Biology 2022Quote: ... and samples were heated at 95°C for 5mins to denature protein and later loaded onto a 4-12% Bis-Tris gel (Thermo Fisher Scientific, NuPAGE, NP0322BOX). Proteins from the gel were transferred to 0.2 μm nitrocellulose membrane (Bio- Rad ...
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bioRxiv - Immunology 2021Quote: ... and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen, catalog no. AM2694), and the resulting RNA/DNA was tested for viral copies by qRT-PCR ...
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bioRxiv - Microbiology 2019Quote: ... containing 10 μg/ml of the c-di-AMP binding protein CpaASP to each well of a 96 well NUNC MaxiSorp plate (Thermo Scientific, Waltham, MA, USA) and the plate was incubated for approximately 18 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... containing 10 μg/ml of the c-di-AMP binding protein CpaASP was aliquoted into each well of a 96 well NUNC MaxiSorp plate (Thermo Scientific, Waltham, MA, USA), which was then incubated for 18 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were first boiled with 6x Laemmli SDS buffer at 95°C for 5 minutes and then proteins were separated on Bolt 4-12% Bis-Tris gels (Thermo Fisher, NWO4122BOX and NWO4127BOX), transferred to Nitrocellulose membranes using the Mini Trans-Blot Cell (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... samples were first boiled with 6x Laemmli SDS buffer ar 95°C for 5 minutes and proteins were separated on Bolt 4-12% Bis-Tris gels (Thermo Fisher, NWO4122BOX and NWO4127BOX). Gels were washed in water ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
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bioRxiv - Cell Biology 2020Quote: ... then in PBS supplemented with 1% BSA and 1% rabbit IgG blocking reagent (ThermoFisher) for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... The slide was washed with PBS before blocking with 10% normal goat serum (Invitrogen) and 40 µg/mL BSA in PBST for 1 hour and then incubated with the mouse anti-zebrafish-Dcst2 antibody (1:25 ...
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bioRxiv - Immunology 2019Quote: ... Tissue sections were treated with BlockAid blocking solution (Thermo Fisher Scientific; cat. no. B10710) for 30 min and incubated with an anti-GXM antibody (1:100 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... were diluted 1:100 in blocking solution containing 1:100 Phalloidin 594 (ThermoFisher #A12381), added to cells ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in blocking solution and secondary Alexa594 goat anti-rat IgG antibodies and DAPI (Invitrogen) in blocking solution for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were immersed in Image-iT FX Signal Enhancer blocking solution (I36933, Invitrogen™) for at least 30 min before overnight incubation with primary antibody solution at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and hybridized with AlexaFluor647-conjugated streptavidin (S21374; Invitrogen; 1:400 diluted in blocking solution) at 37°C for 40 min in a dark humid chamber ...
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bioRxiv - Microbiology 2021Quote: ... in blocking buffer followed by rabbit anti-goat Alexa Fluor 488 (1:500, Invitrogen) in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were blocked with blocking solution (5% milk in 0.1% PBS-Tween20 (Fisher Scientific) for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... and incubated with primary antibodies diluted in SuperBlock Blocking Buffer (Thermo Fisher Scientific 37515) overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... the ovaries were incubated with DAPI (Thermo Fisher; 1 μg/ml in blocking buffer) at 37 °C in a humidified incubator in the dark for 24 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were blocked with blocking buffer (Thermo Scientific™ Pierce™ StartingBlock™; 10108313) for 30 min at room temperature and incubated with primary antibodies (see key resources table ...
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bioRxiv - Bioengineering 2020Quote: ... This solution (10% blocking buffer in PBS) contained 4 µM Hoechst 33342 (Invitrogen H3570) and 6.6 µM phalloidin AF488 (Invitrogen A12379) ...
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bioRxiv - Physiology 2021Quote: ... Secondary antibodies (goat anti-rabbit-Alexa Fluor555, 4 μg/ml in blocking buffer; Invitrogen) were then added for 1 h at room temperature followed by DAPI DNA Stain (1 μg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... before blocking for 1 hour in denatured fatty acid free 1% BSA (Life Technologies). Finally coverslips were washed once with PBS before the addition of platelets ...
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bioRxiv - Neuroscience 2019Quote: ... Blocking solution was then replaced with 1:300 anti-pS6 antibody (ThermoFisher 44-923G) dissolved in blocking solution and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated with SuperBlock T20 (TBS) blocking buffer (Thermo Fisher Scientific, cat. no. 37536) for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: The membrane was incubated in 5 mL SEA BLOCK Blocking Buffer (Thermo Scientific, #37527) for 30 min at RT in a dark container on a rocking mixer ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated in blocking buffer containing secondary antibodies (Alexa Fluor conjugates, 1:400, Invitrogen) for 3-4 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... After blocking of endogenous peroxidase activity using Ultravision hydrogen peroxide block (Thermo Fisher Scientific), endogenous biotin was blocked (Lab Vision Avidin biotin Blocking Solution ...
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bioRxiv - Developmental Biology 2022Quote: ... the slices were incubated in Phalloidin 488 (Invitrogen, dissolved 1:200 in blocking buffer) at 4 °C overnight and washed 3 x for 10 min in PBS with 0.1 % Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were blocked with blocking buffer containing 5% goat serum (ThermoFisher Cat. No. 16210064) in DPBS for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... was combined in blocking solution together with secondary antibodies Alexa Fluor (Thermo Fisher Scientific) 488- (anti-chicken ...
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bioRxiv - Molecular Biology 2022Quote: ... the blot was incubated for 2 h in Superblock blocking buffer TBS (Thermo Scientific), followed by incubation with a 1:5000 dilution of IRDye800CW Streptavidin (LI_COR Bioscience ...
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bioRxiv - Biophysics 2022Quote: ... followed by a rinse with PBS and block with BlockAid blocking solution (Thermo Fisher) for 0.5 −1 h ...
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bioRxiv - Cell Biology 2024Quote: ... sections were blocked with 0.4% Triton X-100 and IHC/ICC blocking buffer (Invitrogen) followed by incubation of primary antibody for 1.5 hours at room temperature or 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were blocked in blocking buffer consisting of 5% normal goat serum (Invitrogen) in PBS for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated with Alexa Fluor - conjugated streptavidin (1:1000 in blocking solution, Invitrogen) overnight at RT ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated in a blocking solution containing 3% bovine serum albumin (Gibco), 0.1% Triton X-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μg/mL of E-cadherin functional blocking antibody (HECD-1, #13-1700, ThermoFisher) was added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... the fixed cells were incubated in a blocking solution containing 5% goat serum (Gibco) and 0.3% Triton™ X-100 (Sigma-Aldrich ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... Secondary antibody (diluted in blocking solution) was (Donkey α-Rabbit, Invitrogen #A21206, 1:2000) and incubated for 2 days at RT while gentle rotating ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 hour of blocking with normal goat serum (NGS) (5%, Invitrogen, #10000C). Primary antibodies raised against NaV1.1 (1:500 ...