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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Ionized peptides were analyzed with an Orbitrap Eclipse mass spectrometer (Thermo Fisher) using the sceHCD-MS2 fragmentation method ...
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bioRxiv - Biochemistry 2024Quote: ... The tryptic peptide solutions were cleaned up by C18-StageTips (Thermo Fisher) and the volume was reduced to approximately 10 µL in a SpeedVac ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were separated on a 50 cm EASY-Spray column (Thermo Scientific), which was assembled on an EASY-Spray source (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Desalted peptides were labeled with TMT 10-plex reagents (Thermo Fisher Scientific). Dried peptides for each sample were dissolved in 100 μL of 50 mM TEAB and each 0.8 mg vial of TMT reagent dissolved in 41 μL of anhydrous acetonitrile was added ...
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bioRxiv - Molecular Biology 2024Quote: Peptides were labelled with the TMT-11plex or TMTpro reagents (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The digested peptides were desalted using Pierce C18 tips (Thermo Fisher Scientific), dried ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were analyzed on a Q Exactive Plus mass spectrometer (Thermo Scientific), equipped with a nanoelectrospray ionization source ...
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bioRxiv - Systems Biology 2024Quote: ... The peptides were analyzed on a Fusion Lumos mass spectrometer (Thermo Scientific) using a 2 cm Acclaim™ PepMap™ 100 C18 HPLC trap column (Thermo Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... The resulting peptides were desalted using Solaµ SPE HRP plates (Thermo Scientific).
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bioRxiv - Microbiology 2024Quote: ... Peptide concentrations were determined with the Pierce Micro BCA assay (Thermo Scientific) using an Epoch2 microplate reader (Biotek ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were trapped on a PepMap™ Neo Trap column (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Desalted peptides were lyophilized in a SpeedVac (Thermo Fisher Scientific, MA, USA) for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: The list of peptide IDs generated based on SEQUEST (Thermo Fisher Scientific) search results were filtered using Scaffold (Protein Sciences ...
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bioRxiv - Molecular Biology 2024Quote: Peptides were loaded onto a 2 cm C18 trap column (ThermoFisher 164946), connected in-line to a 15 cm C18 reverse-phase analytical column (Thermo EasySpray ES904 ...
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bioRxiv - Microbiology 2020Quote: LC-MS/MS analysis of MV-C specific protein complexes was performed on a Q ExactiveTM Plus Mass Spectrometer (Thermo Fisher Scientific, USA) coupled with a Proxeon EASY-nLC 1000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... washed 3x with modified M63 and subjected to 3 cycles of freezing at −20°C and thawing before being lysed with B-PER Complete Bacterial Protein Extraction Reagent (from Thermo Scientific, Cat # 89821) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The phage/serum mixtures were rotated overnight at 4°C after which 40 μL of a 1:1 Protein A/G coated magnetic bead mixture (Invitrogen, #10002D and #10004D) was added to each well and rotated for an additional 4 hours at 4°C to capture all IgG ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 5 min at 95 °C and 30 μg of protein loaded onto Bolt™ 4-12% Bis-Tris Plus precast gels (Thermo Fisher Scientific). Electrophoresis was performed at 180 V constant in 2-morpholin-4-ylethanesulfonic acid (MES)-SDS running buffer (50 mM MES ...
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bioRxiv - Microbiology 2021Quote: ... Cdk1 and its associated proteins were co-purified using the Pierce™ c-Myc-Tag IP/Co-IP Kit (Thermo Scientific, Rockford, IL). Cell lysates from the CDK1-Myc strain were used as a negative control ...
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bioRxiv - Biochemistry 2021Quote: Protein samples were heated for 5 min at 95 °C and separated by electrophoresis in NuPAGE™ 4-12 % gels (ThermoFisher, Cat# NP0329BOX). RNA samples were heated for 2.5 min at 95 °C and separated by electrophoresis in denaturing 9 % acrylamide (19:1 ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were heated at 70 °C for 10 minutes before they were loaded on a NuPAGE™ 12% gradient Bis-Tris Protein Gel (ThermoFisher Scientific, UK) and allowed to run at a constant current of 125 V ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 4°C to remove debris and lysate protein concentration was measured by BCA assay (Thermo Fisher Scientific, Waltham, MA). Protein was reduced with 5 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2020Quote: ... or the same amount of control IgG (R&D, Cat. AB-105-C) together with 40 μl of Dynabeads Protein G (Thermo Scientific, Cat. 10003D) at 4 °C overnight ...
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bioRxiv - Genomics 2021Quote: ... Cells were incubated with the transfection complex in a tissue culture incubator (37°C, 5% CO2) and after 24h protein lysate was extracted using RIPA Lysis and Extraction Buffer (Thermo Fisher cat # 89900) containing cOmplete™ Protease Inhibitor Cocktail (Sigma Aldrich cat # 4693116001) ...
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bioRxiv - Biochemistry 2021Quote: ... minus the 21 C-terminus residues was used as a starting material for protein expression in insect cells (Sf9, Invitrogen, Burlington, ON, Canada). We employed the MultiBac (Geneva Biotech ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific, Carlsbad, CA, USA) and 20 μL of a 50% slurry of sepharose Protein A (Rockland Immunochemicals ...
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Molecular Biology 2022Quote: ... and anti-IgG (negative control) nutating overnight at 4°C followed by the addition of Dynabeads™ Protein A/G (Invitrogen, 10002D/10004D) and rocking for 4 h at 4°C ...
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bioRxiv - Genetics 2022Quote: ... centrifuged at 10000g for 20 min at 4°C and protein levels measured from the supernatant using BCA kit (Thermo Fisher Scientific, UK). 60μg of protein lysate was incubated with 3.6mM ASA in final volume of 50μl ...
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bioRxiv - Biochemistry 2024Quote: ... 1.5-mL microcentrifuge tubes and proteins were denatured for 5 minutes at 100 °C on a Reacti-Therm Single-Block Heating module (ThermoFisher Scientific, Waltham, MA). At room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were clarified by centrifugation for 15 min (21.000 x g and 4 °C) and protein content in the supernatant was determined using a Pierce™ BCA assay kit (Thermo Scientific, USA). Cell lysates were diluted to a final concentration of 2 mg protein/ ml in native lysis buffer and kept on ice until used freshly.
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bioRxiv - Microbiology 2024Quote: ... Filtered supernatant was concentrated approximately 10-fold by spinning at 4°C 2000 RCF for 30 min using a Pierce Protein Concentrator (ThermoFisher, Cat. No. 88537) and then virus aliquots were stored at −80°C and titrated as described in Crawford et al.73 The titers of the concentrated GPC-pseudotyped virus were typically in the range of 1.5×105 to 2×105 transduction units per mL ...
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bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
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bioRxiv - Immunology 2023Quote: ... Lysate samples were clarified by centrifugation at 15,000 rcf for 30 min at 4°C and their protein concentration was determined by BCA assay (Thermo Scientific TM Pierce TM). Samples normalized to equal amounts were denatured in Laemmli’s buffer at 95°C for 10 min and resolved by SDS-PAGE using Criterion TM TGX TM Midi protein gels (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... spun in a microfuge at 13,000 rpm for 20 minutes at 4°C and supernatant protein concentration determined using the BCA (Thermo Fisher Scientific, Rockford, IL) or Bradford (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Elution of protein bound to beads was incubated at 95 °C for ten minutes in 2x NuPAGE® LDS sample loading buffer (Life Technologies, NP0008) containing 4% lithium dodecyl-sulfate (LDS ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates were cleared through centrifugation (14,000 g for 10 min at 4°C) and total protein content was quantified with a Bicinchoninic acid assay (BCA) Assay (Thermo Scientific Pierce, 23227) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysates were cleared at 12.000 rpm at 4 °C for 10 min and 40 μg of proteins were loaded onto 10% polyacrylamide gel (Tris-glycine gels, Thermo Fisher Scientific, USA) and then transferred onto polyvinylidenefluoride (PVDF ...
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bioRxiv - Biochemistry 2024Quote: ... samples were clarified by centrifugation at 13.000 rpm for 15 min at 4 °C and protein concentration was determined using the BCA assay (Thermo Scientific, Rockford, IL, USA). Equal amounts of protein (40 – 50 μg ...
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bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... The protein denaturation was monitored by obtaining the fluorescence signal by increasing the temperature from 22 °C-95 °C at 0.5 °C/minute rate using QuantStudio 3 real-time PCR (ThermoFisher). The melting temperature (Tm ...
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bioRxiv - Cancer Biology 2023Quote: ... and patient-derived cell cultures (P022.C, P024.C, P030.C) were cultured in Dulbecco’s modified Eagle medium (DMEM, #41966, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours with rotation ...
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bioRxiv - Immunology 2022Quote: ... Protein A or protein G Dynabeads (Invitrogen) were prepared according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Protein A and Protein G Dynabeads (Invitrogen) premixed at 1:1 ratio were used for pre-clearing for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Synthetic Biology 2021Quote: ... A melting curve was generated by monitoring fluorescence at 465 nm/610 nm excitation/emission wavelengths while increasing temperature from 10 °C to 90 °C at a rate of 0.06 °C/s on an Applied Biosystem ViiA 7 instrument (Life Technologies). To calculate Tm values ...
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bioRxiv - Bioengineering 2023Quote: ... The protein samples were heated from 25 °C to 95 °C at a rate of 0.3°C/sec in a StepOnePlus real-time PCR system (Applied Biosystems), and fluorescence was captured in the ROX channel ...