Labshake search
Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... retinas were incubated with secondary antibodies at 4°C for another 2-3 days: goat anti-chicken antibody conjugated to Alexa Fluor 488 (1:1000; A11039, Invitrogen), goat anti-rabbit 555 (1:1000 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Slides were then incubated for 1 hour in 2-3 drops per slide of HRP-conjugated streptavidin (Alexa Fluor 594 Tyramide Superboost Kit, Invitrogen), washed 3 times for 10 minutes each in 1x PBS ...
-
bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...
-
bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
-
bioRxiv - Immunology 2020Quote: ... for 2 days, then passaged 1:2 and cultured overnight in R10 medium (1X Roswell Park Memorial Institute (RPMI, (Life Technologies SAS), 10% fetal calf serum ...
-
bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
-
bioRxiv - Cancer Biology 2024Quote: ... The lungs were perfused with PBS through the right ventricle and inflated with 1 to 2 ml of 2% agarose (Thermo Fisher, 16550100) dissolved in PBS by trachea ...
-
bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...
-
bioRxiv - Microbiology 2022Quote: ... or in 2 ml liquid MSgg with 7-d-old tomato seedlings in a 6-well plate (Thermo Scientific). Each well contained one seedling ...
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto a nitrocellulose membrane using iBlot 2 gel transfer device (7 min at 25 V, ThermoFisher). Membranes were incubated for 1 hour at room temperature (RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher), 200 μM L-ascorbic acid (Sigma ...
-
bioRxiv - Biophysics 2023Quote: ... The reaction mixture was then transferred to 2 ml ZebaSpin MWCO 7 kD desalting column (Thermo Fisher Scientific, USA) to separate the labeled protein from the free dye ...
-
bioRxiv - Microbiology 2024Quote: Neutrophils were resuspended in HBSS with Ca2+ and Mg2+ containing 10 μM 2’,7’- dichlorodihydrofluorescein diacetate (DCF) (Molecular Probes) to a final concentration of 1×107 cells/ml and gently agitated for 10 minutes at room temperature ...
-
bioRxiv - Biophysics 2024Quote: COS-7 cells (ATCC CRL 1651™) and HeLa cells (ATCC CCL-2™) were grown in DMEM (Gibco) supplemented with 10% bovine fetal serum ...
-
bioRxiv - Cell Biology 2022Quote: ... dihydro-chloride (DAPI) (Invitrogen. Cat. Nº: D3571) at 0.5 µg/µl ...
-
bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...
-
bioRxiv - Neuroscience 2023Quote: ... An imaging window was constructed from three layers of microscope cover glass (1 x 5 mm, 2 x 3 mm diameter, Fisher Scientific, no. 1) joined with a UV-curable optical glue (NOR-61 ...
-
bioRxiv - Cell Biology 2021Quote: ... The βarr1/2 siRNA (5’-ACCUGCGCCUUCCGCUAUG-3’) and a scrambled siRNA (control, 5’-UGGUUUACAUGUCGACUAA-3’) (Dharmacon) were transfected by RNAimax (Invitrogen) according to the instructions of the manufacturer ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the full volume of media in each well was pipetted gently 4-5 times and added to 1 mL of FACS buffer containing 3 uM DAPI (PBS pH 7.4, 2–5 mM EDTA, 0.1% BSA, 3 uM DAPI (Thermo Scientific #62247)) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The relative expression of each target was calculated using the relative quantification method (2-ΔΔCT) with RNA18S (5’-TGTGGTGTTGAGGAAA-GCAG-3’ and 3’-TCCAGACCATTGGCTAGGAC-5’; Invitrogen) as internal control ...
-
bioRxiv - Cell Biology 2024Quote: ... macrophages in 2 24-well dishes were incubated for 2 hrs in BMM/CSF1 containing 500 mg*ml-1 LY +/- TRDx10 (Thermo Fisher Scientific; 100 mg*ml-1), TRDx70 (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2020Quote: ... hexanoyl)-2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphoethanolamine) (Invitrogen). Cleavage of PED-6 eliminates a self-quenching effect that results in the release of a BODIPY-FL dye ...
-
bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 2 mM MgCl2 supplemented with 2 μM Ultrapure Bovine Serum Albumin (Invitrogen) for 10 min at 25 °C ...
-
bioRxiv - Immunology 2021Quote: ... 2 mM Glutamax and 50 μM 2-mercaptoethanol (Sigma) (all from Thermo Fisher unless stated otherwise).
-
bioRxiv - Systems Biology 2022Quote: ... for 2 h at 37 °C with 2 ul of FastDigest Mph1103I (ThermoFisher cat. no. FD0734) and 2 ul of FastDigest Eco105I (ThermoFisher cat ...
-
bioRxiv - Systems Biology 2022Quote: ... for 2 h at 37°C with 2 ul of FastDigest FspAI (ThermoFisher cat. no. FD1664) and 2 ul of FastDigest PmeI (ThermoFisher cat ...
-
bioRxiv - Cell Biology 2021Quote: ... PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-amino-carbamoyl)methoxy)-phenyl)oxazole] (ThermoFisher Scientific, USA) was added to a final concentration of 330 µM ...
-
bioRxiv - Systems Biology 2022Quote: ... for 2 h at 37 °C with 2 ul of FastDigest BamHI (ThermoFisher cat. no. FD0054) and 2 ul of FastDigest SbfI (ThermoFisher cat ...
-
bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
-
bioRxiv - Immunology 2020Quote: ... IL-2 was measured from culture supernatants using a mouse IL-2 ELISA kit (Thermo Scientific).
-
bioRxiv - Genetics 2020Quote: 10T1/2: C3H10T1/2 cells were grown using the following media recipe: DMEM (ThermoFisher Scientific, 11965118), 10% FBS (ThermoFisher Scientific 26140079) ...
-
bioRxiv - Neuroscience 2020Quote: ... Nuclei were stained with 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml, Molecular Probes). The sections were mounted using a fluorescence mounting medium (DAKO ...
-
bioRxiv - Plant Biology 2022Quote: ... 2 μg of total RNA was treated with 2 units of DNase I (Thermo Fisher Scientific) and then used as a template for cDNA synthesis with RevertAid Transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... in 2% TBS-T (1x Tris Buffered Saline, pH 7.6 with 2% Triton-X100, Fisher Scientific) for 1 h ...
-
bioRxiv - Immunology 2024Quote: ... 25 mM N-2-hydroxy-ethylpiperazine-N′-2-ethanesulfonic acid (HEPES, Gibco, Grand Island, NY, USA), 100 U/mL penicillin and streptomycin (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... 100 ng purified DNA library was ligated with 2 μl barcode and 2 μl P1 (ThermoFisher). NGS analysis was performed using an Ion Torrent PGM (ThermoFisher) ...
-
bioRxiv - Genomics 2023Quote: ... The nuclear stain was 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/mL; Molecular Probes). Sections were imaged digitally using a slide scanner (Olympus VS-120 Slide scanner ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 2 mmol/L ethylenediaminetetraacetic acid) and stained with 2 μg/mL propidium iodide (PI; Invitrogen). PI was added just before the analysis ...
-
bioRxiv - Cell Biology 2023Quote: ... Equal volume of SYTOX Green (5 μM final in HBSS without Ca+2/Mg+2, Invitrogen) was added to each sample and incubated in the dark for 10 min ...
-
bioRxiv - Systems Biology 2023Quote: ... We blocked cysteine residues using 2 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Thermo Fisher Scientific) at 37 °C for 30 min followed by alkylation with 10 mM 2-iodoacetamide (IAA ...
-
bioRxiv - Microbiology 2024Quote: ... heparin was biotinylated by mixing 2 mg heparin and 2 mg amine-PEG3-Biotin (Thermo Scientific), followed by the addition of 10 mg NaCNBH3 ...
-
bioRxiv - Systems Biology 2024Quote: ... Cysteine residues were blocked by 2 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Thermo Fisher Scientific) at 37°C for 30 min followed by alkylation with 10 mM 2-iodoacetamide (IAA ...
-
bioRxiv - Bioengineering 2024Quote: ... EBMTM-2 basal media was supplemented with 2% exosome-depleted FBS (Thermo Fisher Scientific, Cat. # A2720803). Media was exchanged every 24-48 hours and cells were used between passages 3-6 ...
-
Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... in neuronal differentiation medium (Neurobasal-A supplemented with 2% B-27, 2 mM L-glutamine (Gibco), 200 µM L-ascorbic acid 2-phosphate ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were washed with FACS buffer (PBS supplemented with 2% FBS and 2 mM EDTA (Gibco)) and were centrifuged at 400 g for 5 min at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... RNA (2 μg) from each sample was treated with TURBO™ DNase (2 Units, Thermo Scientific), and subjected to cDNA synthesis using iScript cDNA synthesis kit (Bio-Rad) ...
-
bioRxiv - Neuroscience 2024Quote: iPSC-derived neurons were incubated in 2.5 μM Fura-2-acetoxymethyl ester (Fura-2-AM, Invitrogen) for 50 min at 37°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... along with 2 μl of 10 × Buffer and 2 μl of T7 RNA polymerase (Invitrogen AM1354). The sample was mixed gently and incubated at 37°C for 3 h ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with 4 μM fura-2 acetoxymethyl ester (fura-2/AM, Invitrogen, Cat# M1291) in HEPES-buffered solution (137 mM NaCl ...
-
bioRxiv - Microbiology 2022Quote: The PfHDAC1-2xFKBP-GFP knockin NF54 line parasitized RBCs (mock or 100 nM dihydro artemisinin treated from ∼21-24 HPI/3 hours) were crosslinked using 1% formaldehyde (Thermo Scientific, 28908) for 10 mins at RT ...