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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... For the preparation of the BGLF2-KO virus, HEK293/EBV(dBGLF2) cells (Konishi et al., 2018) were transfected with BZLF1 expression plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Linearized plasmids containing the ItypOrco gene were transfected into HEK293 cells containing a tetracycline-inducible repressor (TREx) using Lipofectamine 2000 (Thermo Fisher Scientific) (Corcoran et al ...
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bioRxiv - Microbiology 2020Quote: ... HEK293-EBV B(+/−) S13+/− cells were transfected with pCDNA3-BZLF1 and pCDNA3-BALF4 using TurboFect Transfection Reagent (Thermo Scientific, Waltham, MA, USA). Old medium was changed with fresh medium in one day post transfection ...
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bioRxiv - Immunology 2021Quote: HEK293 adherent cells grown to a confluency of 70–90% were transfected using a Lipofectamine 3000 Reagent Kit (L3000075, Invitrogen, Waltham, MA). Transfection procedures were adapted from the product manual ...
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bioRxiv - Microbiology 2022Quote: ... Titration of progeny viruses from all HEK293 cells was done in Vero-E6 cells cultured in 16-well chambered slides (Thermo Fisher Scientific), as described below ...
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bioRxiv - Genetics 2022Quote: ... These plasmids were inserted into the same place in the genome using the Flp-In recombinase technology to generate Flp-In HEK293 (Thermofisher, cat. #R78007) stable cell lines ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were grown in a monolayer on 10 cm culture dishes in Dulbecco’s Modified Eagle Medium (DMEM) (ThermoFisher Scientific, Waltham, MA) supplemented with fetal bovine serum (10% v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing either dCas9-KRAB or a targeting sgRNA were generated by transfection of HEK293 cells with Lipofectamine 2000 (Thermo Fisher Scientific). Cells were cotransfected with the packaging plasmid pCMV-dR8.91 ...
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bioRxiv - Systems Biology 2021Quote: HEK293 cells were maintained at 37°C and 5% CO2 in phenol red-free Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal calf serum (Sigma-Aldrich (MilliporSigma)) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... HEK293 cells were a gift from Dr Harvey McMahon and were cultured in high-glucose DMEM supplemented with GlutaMAX (Thermo Fisher, 31966047) and 10% FBS (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: Cell lines were made by co-transfecting plasmids with lentiviral packaging vectors (VSVG and SPAX2) into packaging cells (HEK293-FT) using Lipofectamine 2000 (Thermo Fisher Scientific). Viral supernatants were collected ...
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bioRxiv - Biophysics 2021Quote: The HEK293 cell line subclone TS201A was a gift from Vladimir Yarov-Yarovoy and was maintained in DMEM (Gibco Cat# 11995-065) with 10% Fetal Bovine Serum (HyClone ...
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bioRxiv - Neuroscience 2022Quote: HEK293-T cells of low passage (<25) were kept in Dulbecco′s Modified Eagle′s Medium (DMEM, Thermo Fisher Scientific #31966-047) containing 10% fetal calf serum (FCS ...
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bioRxiv - Biochemistry 2022Quote: ... 12 pmol crRNA:tracrRNA duplex was combined with 12 pmol recombinant HiFi Cas9 (IDT) and reverse transfected into HEK293 Flp-In cells using Lipofectamine CRISPRMAX reagent (Thermo Fisher Scientific) following IDT protocols ...
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bioRxiv - Genetics 2022Quote: ... pgLAP1-3MYC-BioID2 subnit plasmids were then transformed and stably integrated into HEK293-Flp-In-T-REx cells using the manufacturer’s protocol (ThermoFisher Scientific, catalogue #R78007).
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bioRxiv - Cell Biology 2024Quote: Measurement of extracellular Ca2+ clearance by mitochondria from digitonin-permeabilized HEK293 cells was performed using the membrane impermeable Ca2+ indicator Calcium-Green-5N (Life technologies, C3737). Cells were harvested at a density of 500000 cells/mL in growth medium supplemented with 20 mM HEPES (pH 7.2/NaOH) ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: The plasmids coding for pro-BMP9 and pro-BMP10 were co-transected in equal amounts into HEK293 cells (expi293, Invitrogen/Thermo Fisher Scientific) and after five days ...
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bioRxiv - Cell Biology 2022Quote: ... into the Flp-In™ T-REx™ HEK293 cell line or Jaw1 KO cells (#R78007; Thermo Fisher Scientific, Waltham, MA, USA). After incubation for 24 h ...
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bioRxiv - Physiology 2023Quote: ... mVenus:FL_ Proline and mVenus:2x linker proteins were transfected into HEK293 (Catalog CRL-1573, ATCC) cells with lipofectamine 3000 transfection reagent (Catalog L3000008, Thermo Fisher Scientific). 24h post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Expression vectors of SLC22A10 orthologs were introduced into HEK293 Flp-In cells either through transient transfection or stable transfection using Lipofectamine LTX (Thermo Fisher Scientific). For transfections in a 48-well plate (seeding density ...
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bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into HEK293 cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: S1PR1-Flag and CD69-StrepII were separately expressed using baculovirus-mediated transduction of mammalian HEK293S GnTI− cells (ATCC CRL-3022) in a medium containing FreeStyle 293 (Gibco Cat# 12338018) supplemented with 2% charcoal-dextran stripped fetal bovine serum (Gibco Cat# 12676029) ...
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bioRxiv - Genetics 2023Quote: ... Lentiviruses were produced transfecting HEK293 cells growing on a T75 flask with 20ug of total DNA and Lipofectamine 2000 (Thermo Fisher Scientific) for 16h ...
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bioRxiv - Cell Biology 2023Quote: ... pLVX-DsRed-Spartin plasmid together with psPAX2 and VSV-G plasmid were transfected into HEK293 cells using Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) for generating lentivirus ...
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bioRxiv - Cell Biology 2024Quote: ... was added for some experiments. HEK293 cells (a gift from H. Choe) used for generation of lentiviral particles were maintained in DMEM (Gibco #11995-065) supplemented with 10% FBS and antibiotic–antimycotic reagent (Gibco #15240-062 ...
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bioRxiv - Biochemistry 2024Quote: ... Expi293F cells (derived from HEK293 cells) were grown in suspension and shaken at 125 rpm using serum-free Expi293TM expression medium (Gibco, Cat#A1435101) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: The transfection of HeLa and HEK293 cells with siRNAs (100 pmol each) was performed using Lipofectamine RNAi max (Invitrogen-Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of HeLa and HEK293 cells with siRNAs (100 pmol each) was performed using Lipofectamine RNAi max (Invitrogen-Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: ... MDA-MB-231 human breast cancer cells were cultured in DMEM/F12 (1:1) media52 (Thermo Fisher Scientific, Life Technologies, Item #11330-032) supplemented with 10% Fetal Bovine Serum (Atlanta Biological ...
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bioRxiv - Bioengineering 2021Quote: ... MDA-MB-231 (ATCC) and MCF7 (ATCC) human mammary cancer cells were both cultured in 1:1 DMEM/F-12 (Thermo Fisher Scientific, USA) media supplemented with 10% fetal bovine serum (Atlanta Biological ...
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bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained for 30 min on ice using AlexaFluor700-conjugated anti-Human CD54/ICAM-1 (clone 1H4, Life Technologies, #MA528553, 1/75), PE-Cy7-conjugated anti-Human CD106/VCAM-1 (clone STA ...
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bioRxiv - Microbiology 2021Quote: ... Cell suspensions prepared by enzyme-free CDS were kept in PBS-5% FCS and incubated on ice for 30 min with rabbit polyclonal anti-human HVEM (R140; 1:500) (Terry-Allison et al., 1998) followed by visualizing HVEM with anti-rabbit AF488 (Life Technologies; 1:200). The size of the epidermal samples mostly allowed only the analysis of nectin-1 expression ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or antibodies was detected using an AF633 donkey goat anti-human IgG antibody (Iinvitrogen, A21091, 1/1000) and dead cells were stained using Live/Dead stain (Invitrogen, 15560607, 1/1000). Following 3 washes with PBS containing 0.5% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma human glucagon was measured by the human glucagon ELISA kit (Thermo Scientific, Frederick, MD). Exendin-4 plasma levels were measured using the exendin-4 EIA kit (Phoenix pharmaceuticals ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 50 μL fresh FACS buffer and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Immunology 2022Quote: ... Total IgG in human sera was measured using a commercial human IgG ELISA kit (Fisher Scientific) according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... All human CD8+ T cells were activated with anti-human CD3/CD28 dynabeads (11131D, Thermo Scientific) at a 1:1 cell-to-bead ratio and cultured in complete RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Neuroscience 2023Quote: ... Secreted Aβ42 peptides were quantified using the Human Amyloid β1-42 human ELISA kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Fresh human BAL cells and frozen human PBMCs were stained with Aqua live/dead dye (Invitrogen) for 20min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of human CCDC22 and CCDC93 and full-length human DENND10 were ordered as GeneStrings (ThermoFisher) with the gene codon optimized for E ...
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bioRxiv - Cell Biology 2023Quote: ... Purified human neutrophils and T cells were cultured in Human Plasma-Like Medium (HPLM; ThermoFisher Scientific) with 5% heat inactivated FBS supplemented ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were thawed and activated with Human T-Expander CD3/CD28 Dynabeads (Gibco) at a 3:1 bead:cell ratio in complete medium (RPMI 1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2021Quote: ... human melanocyte growth supplement (HMGS, Gibco), gentamicin (Gibco ...