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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... coverslips were mounted onto glass slides with mounting media containing DAPI (4’, 6-diamidino-2-phenylindole; ProLong Gold, Life Technologies).
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were counter stained with 4′,6-diamidino-2-phenylindole (DAPI) and mounted in ProLongTM Gold antifade mounting media (Invitrogen). Images were captured using Nikon C2 confocal microscope.
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Transient transfections of wild-type or mutated MCM10-expressing plasmids were performed by transfecting 2×106 cells with 6 μg of DNA using Lipofectamine 2000 according to manufacturers’ instructions (Invitrogen).
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with DPBS and nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (300 nM DAPI stain solution in PBS; Invitrogen) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Perkin-Elmer Life Sciences) for 15 min and then stained with 4’,6-diamidino-2-phenylindole (DAPI) before mounting in Prolong Diamond (Invitrogen).
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bioRxiv - Systems Biology 2019Quote: ... for 30 minutes at 37C and plated with 2 million C57Bl/6 mouse embryonic fibroblasts as feeder cells (Gibco A34960) and with serum+LIF media composing of ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were mounted onto glass slides with ProLong Gold Anti-Fade reagent with DAPI (4[prime],6-diamidino-2-phenylindole; ThermoFisher). Images were obtained using an Olympus BX60 inverted microscope equipped with phase and fluorescence optics ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were washed 3 × 5 minutes in PBS and finally mounted onto glass slides using Prolong Gold mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (Thermofisher). Images were acquired via confocal microscopy using a Zeiss LSM-880 confocal microscope (Plan-Apochromat 40x/1.3 Oil DIC UV-IR M27objective ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were mounted on slides using ProLong Gold Antifade Reagent with DAPI (4′,6-diamidino-2-phenylindole) (Thermo Fisher Scientific) and allowed to set overnight ...
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bioRxiv - Microbiology 2021Quote: ... the cells were counterstained in 1 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific GmbH, Bremen, Germany) for 10 min at 46°C ...
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bioRxiv - Genetics 2020Quote: ... then incubated for 2h at room temperature with Alexa Fluor secondary antibodies (S2 Table) and nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Images were captured on a BX51 fluorescence microscope (Olympus ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we stained the formaldehyde preserves with DAPI (4’,6-diamidino-2-phenylindole, final concentration 5 ug/mL, Thermo Scientific, USA), and counted the microbial cells using hemocytometers (DHC-N01 ...
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bioRxiv - Microbiology 2022Quote: ... for 30 minutes at RT followed by a 5-minute incubation with 300 nM 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein lysates were combined with 6× loading buffer containing 2-mercaptoethanol and loaded on NuPAGE 4%-12% Bis-Tris Midi gels (Invitrogen). After separation of protein by SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Hoechst 43222 (H1399) and ProLong Gold Antifade Mountant with 4′,6- diamidino-2-phenylindole (DAPI; P36931) were purchased from Invitrogen.
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bioRxiv - Cell Biology 2022Quote: ... The plates were then washed with PBS 3×10min at RT on a shaking plate and cell nuclei were stained with 20 μg/ml DAPI (4’,6-Diamidino-2-Phenylindole, Invitrogen) in PBS for 15 min ...
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bioRxiv - Biophysics 2022Quote: ... Fractions containing S15 were consolidated and concentrated to ∼2 mg/mL using a 6 mL 3K molecular weight cutoff centrifugal concentrator (ThermoFisher), before being aliquoted and flash-frozen in liquid nitrogen for long-term storage at -80°C.
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed with 1xPBS and incubated with μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies, #D1306). Coverslips were then mounted in Vectashield (LSBio ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were added with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 µg mL-1 in PBS, Invitrogen) and phalloidin conjugated Alexa Fluor dye (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Enteroids were then washed with PBS three times with the addition of 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (1:5000) (Invitrogen) for the final 10 min wash ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in a flow-cytometry buffer and DAPI (4′,6-diamidino-2-phenylindole, 0.5 μg/ml; ThermoFisher #D1306) solution to exclude dead cells ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then fixed in 4% formaldehyde at room temperature for 10 minutes and mounted onto slides using ProLong Gold or Diamond Antifade Mountant with 4′,6-diamidino-2- phenylindole (DAPI) (ThermoFisher). Images were obtained using a Leica SP8 confocal microscope using excitation/emission spectra ...
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bioRxiv - Neuroscience 2022Quote: ... CHO cells were plated at 1 x 106 cells/well in a 6-well plate and cultured for 24 hr in 2 ml of F12 medium (Gibco) supplemented with 10% FBS and 1% Glutamax (Gibco ...
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bioRxiv - Biochemistry 2023Quote: Doxycycline-inducible C2C12 cells were seeded for most treatments at 0.1X106 cells per well in 6-well plates with 2 ml of DMEM [high glucose DMEM with pyruvate (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Bioengineering 2024Quote: ... DAPI (4′-6-diamidino-2-phenylindol) and Myosin Heavy Chain (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™). All images were taken using a confocal microscope (Zeiss LSM 780 Airyscan ...
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bioRxiv - Cell Biology 2024Quote: ... both at 1:300 dilution and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen). Confocal microscopy (Olympus FLUOVIEW FV3000 ...
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bioRxiv - Neuroscience 2024Quote: ... Hippocampal sections were stained with 4’,6-diamidino-2-phenylindole (DAPI) and cresyl violet or fluorescent Nissl (NeuroTrace 435/455 Blue, ThermoFisher). OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg DNA was mixed with 6 µL of FuGENE HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at RT for 10 min before being added to HeLa cells grown in 35-mm glass-bottomed dishes (AGC Technology Solutions ...
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bioRxiv - Genetics 2023Quote: ... ISE6 cells were fixed with 4% paraformaldehyde followed by 1 x PBS washes and the coverslip was mounted on a slide using Antifade gold mounting reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen) and observed under the Nikon W-1 Spinning Disk confocal microscope ...
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bioRxiv - Genomics 2022Quote: ... resuspended in 1 mL NSB with 1:20 dilution of 0.25 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) and FACS sorted ...
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bioRxiv - Molecular Biology 2022Quote: ... the sections were mounted with ProLong Gold with 4′,6-diamidino-2-phenylindole (DAPI) (catalog no. P36935, Thermo Fisher Scientific).
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bioRxiv - Biophysics 2023Quote: ... the samples were added with a DNA-binding dye 4’,6-diamidino-2-phenylindole (DAPI, 1 μg mL-1 in PBS, Invitrogen) along with the secondary antibody solution ...
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bioRxiv - Biophysics 2023Quote: The membrane probes Di-4-ANEPPDHQ and Laurdan (6-dodecanyl-2-dimethylaminonaphtalene) were obtained from Molecular Probes (Eugene, OR, USA) and dissolved in ethanol and dimethyl sulfoxide (DMSO ...
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bioRxiv - Biochemistry 2023Quote: The cells were seeded in 6 well plate and treated the next day using 2 µg of plasmid and 4 µL of lipofectamine 3000 (Invitrogen) for 24 h or 10 nM of siRNA and 5 µL of RNAiMAX (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 3×106 MART-1-transduced T cells were added and incubated for the indicated hours (2, 4 or 6) in “Intermediate” 4K6R medium consisting of RPMI 1640 Medium for SILAC (88365, Thermofisher) with 10% dialyzed fetal bovine serum (15605639 ...
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bioRxiv - Biophysics 2023Quote: ... Adherent semiconfluent HEK293SG cells cultured in DMEM:F12 medium supplemented with 10% FBS were transfected in 6-well plates with 2 μg/well of plasmid DNA complexed with Lipofectamine 2000 reagent (Invitrogen). The cells were incubated for 48 hrs at 37 °C under 7% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed and stained with 1:10,000 DAPI (4=,6-diamidino-2-phenylindole, Thermo Fisher Scientific; catalog #: D3571) for 15 minutes at room temperature before coverslips were applied using PBS + glycerol as mounting medium.
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bioRxiv - Cell Biology 2023Quote: Binucleation analyses and detection of aberrant divisions were performed by heat-fixing cells on a microscope slide at 70 °C before staining with 4’,6-diamidino-2-phenylindole (DAPI) (SlowFade Diamond Antifade Mountant with DAPI, Invitrogen) and Calcofluor (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Bioengineering 2023Quote: PAAm gels prepared on coverslips were transferred to multiwell plates before covering with 0.2 mg/mL sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino)hexanoate (sulfo-SANPAH) (Thermo Fisher) solution ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells at ∼50% confluency in 6-well plates were incubated with 2 mL Opti-MEM-I medium (Gibco, 31985062) supplied with 6 µL Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... a working solution of 0.83 mg/mL solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, 22589; Thermo Fisher) in PBS was prepared from a stock solution of 83 mg/mL sulfo-SANPAH in DMSO (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... in blocking buffer prior to a final incubation with 4′,6-diamidino-2-phenylindole (DAPI) or anti-phalloidin for F-actin (Invitrogen) at 25 °C ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected in 6-well plates with 2 µg of the indicated constructs in the presence of Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with 0.1% Triton X-100 in PBS supplemented with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/ml) (Invitrogen, # 62248) for 30 min ...
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bioRxiv - Physiology 2024Quote: ... CHX-treated cells were harvested at different time points (0, 2, 4 and 6 hours) and processed for immunoblotting with anti-LSD1 (Invitrogen) and anti-RCOR1 (Abcam ...
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bioRxiv - Biophysics 2024Quote: ... DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI) in phosphate buffered saline (PBS) and cells were mounted with ProLong Gold Antifade reagent (Invitrogen).