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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... HRP (ThermoFisher; 1:5000, 60 min at 4°C). Detection was achieved with Pierce™ ECL Western Blotting Substrate using a ChemDoc XR+ (BioRad).
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bioRxiv - Developmental Biology 2019Quote: ... anti-Claudin 4 (Life Technologies, 36-4800, 1:200), anti-β5t (MBL ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 µM Fluo-4 (F14201, Thermo Fisher Scientific) were pre-incubated for 30 minutes prior to image acquisition ...
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bioRxiv - Microbiology 2020Quote: ... 4 mM L-glutamine and 1% penicillin-streptomycin (Invitrogen) in 24-well plates for 7 days ...
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bioRxiv - Genomics 2020Quote: ... with 1 mg/mL Collagenase 4 (Fisher Scientific, #NC9836075), and 10-20 μg/mL DNAse I (StemCell Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-IL-4 (1:500; Thermo Fisher Scientific). Brain sections were then washed 4 × 5 min with PBS followed by overnight incubation at 4°C with the following secondary antibody dilutions in blocking solution ...
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bioRxiv - Cancer Biology 2022Quote: ... 4) Opal 620/anti-TdT (1:8, SEN28, Invitrogen), 5 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mL of ethyl acetate (E196-4, Fisher Scientific) was added to the culture medium and mixed for 1 hour at 28 °C ...
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bioRxiv - Microbiology 2023Quote: ... 1 cm into a 4-12% NuPage gel (Invitrogen) before staining with Coomassie blue (GelCode Blue Safe Protein Stain ...
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bioRxiv - Bioengineering 2023Quote: ... and 4 μM Ethidium Homodimer-1 (Thermo Fisher Scientific) and imaged using a Zeiss Axio Observer ...
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bioRxiv - Microbiology 2023Quote: ... 1 cm into a 4-12% NuPage gel (Invitrogen) before staining with Coomassie blue (GelCode Blue Safe Protein Stain ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 mL of ethyl acetate (E196-4, Fisher Scientific) was added to the culture medium and mixed for 1 hour at 28 °C ...
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bioRxiv - Neuroscience 2023Quote: ... incubated overnight at 4° C (MAP2 1:1000 Invitrogen #MA5-12826 ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μM Fluo-4 AM (ThermoFisher Scientific, F14201) respectively ...
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bioRxiv - Developmental Biology 2024Quote: ... 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPO, Thermo Scientific), and Proteinase K (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting PCR product was then inserted into the 5’ SpeI and 3’ EcoRI sites of the pMT/V5 His-B vector (Invitrogen) containing in-frame mTurquoise2 gene at the 5’ end ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: The HIF-1α-PAS-B domain was coated at 3 μg/ml onto 96-well microtiter plates (Thermo Fisher Scientific) and incubated overnight at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... DiIC18(1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotri-carbocyanine Iodide) and the Alexa 488-labeled fragment B of cholera toxin were purchased from Thermo Fisher scientific (Waltham ...
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bioRxiv - Genetics 2022Quote: ... Hygromycin-resistant colonies emerging on the surface of the overlay after 3–5 days were excised and transferred to MEA amended with 100 µg/mL of hygromycin B (Invitrogen) (MEA+Hyg100) ...
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bioRxiv - Immunology 2021Quote: ... Qβ-VLPs were then re-packaged with B-type 1668 CpGs (5″-TCC ATG ACG TTC CTG ATG CT-3″) with phosphorothioate backbone purchased from (InvitroGen). The re-packaging was confirmed by 1% agarose gel stained with SYBR Safe dye for 30min at 90V ...
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bioRxiv - Neuroscience 2023Quote: ... over 75 min and from 55% to 100% solvent B in 3 min at a constant flow rate of 0.25 μl/min on UHPLC Easy-nLC 1000 (Thermo Scientific) where the LC system was connected to a 23-cm fused-silica emitter of 75 μm inner diameter (New Objective ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Cell Biology 2021Quote: ... diluted cell supernatant was preabsorbed for one hour at 4°C with protein A/G magnetic beads (Invitrogen). The supernatant fraction was incubated overnight at 4°C with an appropriate antibody and protein A/G magnetic beads were blocked with 4% BSA ...
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bioRxiv - Molecular Biology 2020Quote: One hundred micrograms of mitochondrial proteins were separated on 4-16% (w/v) polyacrylamide Native PAGE gels (Invitrogen) and then either transferred to PVDF membranes or subjected to in-gel activity staining as in previously described in (25) ...
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bioRxiv - Microbiology 2022Quote: ... A total of 4 μg of RNA was treated with one unit of DNase I Amplification Grade (Invitrogen) to remove potential contamination with genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... Eluted protein complexes were separated by one-dimensional 4-12% NuPage Novex Bis-Tris Gel (Invitrogen, Cat. NP0321BOX) and stained using the Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500, ThermoFisher 32-2500), Rabbit anti-Myc polyclonal (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were washed 3 times with PBS and incubated for 1h at room temperature with goat anti-rabbit AlexaFluor-568 (ThermoFisher, Invitrogen A-1101, 5ug/ml working solution). After washes with PBS ...
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bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Reverse 5′-CGA AGG TGT GAC TTC CAT G-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Cell Biology 2022Quote: HCT116 cells were cultured in a 6-well plate at 300,000 cells per well in 3 ml McCoy’s 5A medium (Gibco) supplemented with 10% heat inactivated FBS and with 90 UI/mL Penicillin ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 30 ovaries from 3-6 day-old flies using TRIzol (Thermo Fisher Scientific) in three replicates ...
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bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 3 ml was diluted 1:1 with PBS (Thermo Fisher Scientific, Waltham, MA) and kept on ice for purity analysis by flow cytometry ...
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Immunology 2024Quote: In vivo labeling of tumor-infiltrating T lymphocytes (TILs) with 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG, Life Technologies, catalog: N13195) was done by intravenous (retroorbital ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL malt extract (2% w/w) in 4 mL screw neck vials (Fisher Scientific, Loughborough, UK) were inoculated with an agar piece of the G ...
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bioRxiv - Cell Biology 2024Quote: ... adipose tissue (2∼4 cm3) was washed twice with PBS solution (AQ) containing 2% penicillin-streptomycin (Invitrogen), and then cut to 1∼2 mm3 with scissors ...
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bioRxiv - Cell Biology 2021Quote: ... phalloidin Texas red (1:100, 1h, Life technologies #T7471) and anti-alpha-Tubulin-FITC (1:200 ...
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bioRxiv - Pathology 2024Quote: Tear samples were thawed at 4°C on ice and the total protein concentration was measured with a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific, reference ND- ONE-W) by using 2µL of sample ...
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bioRxiv - Cell Biology 2021Quote: Cross-linking experiments of Sgo11-415 and CPCISB10-280 were performed using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific) in the presence of N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Microbiology 2019Quote: ... and then induced to differentiate by culturing for further 3 days in a 3:1 mixture of DMEM and Ham’s F12 media (Invitrogen, Palo Alto, CA) containing FCS (10%) ...
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bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
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bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...
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bioRxiv - Microbiology 2024Quote: ... was added at a 1:2000 dilutions for 1 h at 37 °C, followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...