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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Fipronil Sulfone 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... at 4°C overnight, rinsed in PBS (3 times, 5 min each) and amplified by Alexa Fluor-488 donkey anti-rat antibody (Life Technologies, A21208, 1:600). Sections were mounted with Fluoromount containing DRAQ-5 (1:1000).
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cells were seeded in 6-well plates (5×105/well) were transfected with the indicated siRNA (3 μg) using Lipofectamine® 2000 transfection reagent (Life Technologies, 11668-019) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and HmlΔ-Reverse primer: 5’-GTTTAATTGTATACACAGGAAAATC-3’) were amplified from Drosophila genomic DNA and ligated into the pENTR™/D-TOPO™ vector (Invitrogen: Cat#K240020) for Gateway cloning ...
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bioRxiv - Cell Biology 2020Quote: ... 3 % SDS and 5 mM EDTA) and protein concentrations were determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Plant Biology 2022Quote: ... The cells were then incubated at their respective temperatures for 3 minutes prior to addition of 5 μM Sytox Green (Thermo Fisher Scientific, Loughborough, UK). All treatments were then incubated at 20 °C 15 min in darkness ...
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bioRxiv - Molecular Biology 2022Quote: ... A final 5-minute 2xSSC wash was performed before the coverslips were mounted on a pre-cleaned frosted glass slide (Thermo Fisher, 12-552-3) with ProLong Diamond antifade reagent with DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: P29 mice were anesthetized with isoflurane (1–3%) and 50 nl of the retrograde tracer cholera toxin subunit B (5% wt./vol in PBS, ThermoFisher Scientific, Cat # C-34775) were injected at 10nl/sec into the left SLM (ML ...
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bioRxiv - Cell Biology 2023Quote: ... We then used the linearized MLM3613 as template to synthesize the 5′ capped and 3′ polyA-tailed Cas9 mRNA using the mMESSAGE mMACHINE® T7 Ultra Kit (ThermoFisher, cat no: AM1345) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... 15 ug of psPAX2 and 5 ug pMD.G plasmid were vortexed with 40 ul P3000 reagent in 1.5 ml OptiMEM (ThermoFisher, 31985070). Then 40 ul Lipofectamine was added to 1.5 ml OptiMEM and briefly vortexed ...
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bioRxiv - Genomics 2022Quote: ... Plasmids were mixed in a 3:1 weight ratio of total plasmid DNA:PEI in 4 mL of either OptiMeM™ (Gibco cat. # 31985-062) or serum-free DMEM per flask and then shaken vigorously for 30 sec ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed 3 times with 200 µL PBS for 4 min before secondary antibodies and 1 µg/mL Hoechst (Thermo Fisher Scientific #33342) were added in 35 µL per well and incubated for 1 hour at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... 100% methanol (Fisher Scientific, #A452) and 70% ethanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Pathology 2020Quote: ... and resolved on 4-12% Bis-Tris Plus Bolt™ gels at 4-5 ug of protein per lane in MES SDS Running Buffer (Invitrogen). Target-protein signal was detected with Horseradish Peroxidase (HRP ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μM Lysotracker Red DND-99 (Invitrogen) was added to each plate 1 hour prior to cell harvest ...
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bioRxiv - Cancer Biology 2019Quote: ... unmigrated cells were removed from the inside of the inserts with a cotton swab and the cells were fixed in ice-cold 100% methanol for 5 min and stained with 1% w/v crystal violet (ThermoFisher Scientifics) for 3 min ...
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bioRxiv - Microbiology 2021Quote: 3 μg of in vitro-transcribed RNA derived from the pCrPV-3 or chimeric clones was transfected into 3 × 106 S2 cells using lipofectamine 2000 reagent (Life Technologies) per the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S ‘2P’ and HexaPro IMAC elution fractions were concentrated to ∼ 1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1.5ml of SD/-Leu/-His liquid medium (plus 3 mM 3-AT) were incubated in a 12-well microplate (Thermo Scientific) at 30°C for 96 hours (h) ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of xopX and its 14-3-3 protein binding motif mutants were cloned by Gateway cloning (Invitrogen, California) from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP ...
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bioRxiv - Cancer Biology 2020Quote: ... The purified RNA obtained from IVT was labelled with biotin at the 3’end using the PierceTM RNA 3′End Desthiobiotinylation Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... RNA was desiccated to 3 μl and denatured for 3 minutes at 72 °C in the presence of 2.4 mM dNTP (Invitrogen Carlsbad, USA), 240 nM dT-primer* (Metabion ...
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bioRxiv - Immunology 2020Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S-2P IMAC elution fractions were concentrated to ~1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis induction was determined by activation of Caspase 3 and 7 using the CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen).H460 cells were plated at 5 × 103 cells/well in black 96 well plates with clear bottoms (Costar ...
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bioRxiv - Cell Biology 2022Quote: 6xHis-14-3-3 isoforms were expressed in BL21(DE3) bacterial cells and purified in batch by IMAC with ProBond™ resin (Invitrogen). After elution with imidazole ...
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bioRxiv - Cancer Biology 2022Quote: ... Pierce premium grade N-hydroxysulfosuccinimide (sulfo-NHS, PG82071) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 22980) were purchased from Thermo Fisher Scientific.
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bioRxiv - Neuroscience 2021Quote: ... A 3 µm reverse phase column (BDS-Hypersil-C18, 150 × 3 mm, pore size 130 Å, Thermo Fisher Scientific, Waltham, USA) and an ECD-3000RS configuration with two coulometric cells (6011RS ultra-analytical cell ...
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bioRxiv - Physiology 2019Quote: ... 0.3 μL of 20X GE Sample Loading Reagent (Fluidigm) and 3 μL of TaqMan® Gene Expression PCR Master Mix (ThermoFisher). Each 6 μL assay master mix (AMM ...
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bioRxiv - Neuroscience 2019Quote: ... sections from post mortem controls (n=3) and 22qDS patients (n=3) were sectioned and mounted on positively charged slides (ThermoFisher Scientific). Sections were deparaffinized by washing in xylene followed by washing in 100% ...
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bioRxiv - Genetics 2020Quote: AAG AGC TCT TCA GAA ATG GAT GCC ATG TGT GTC TCC TCT TCC TGA-3’) derived from the intron 10 of PIK3C2B were biotin-labeled using Biotin 3’-End DNA Labeling Kit (Thermo Scientific). Biotin-labeled ssODNs were then annealed with reverse complimentary ssODNs to generate biotin-labeled probes ...
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bioRxiv - Cell Biology 2021Quote: ... or si-hsa-CHST15_0003 (n□=□3) or si-hsa-TNFRSF21_0001 (n□=□3) for 24□hours was carried out by using human Clariom™ Sassay (ThermoFisher Scientific) at the Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked for 3 h at room temperature in PBS with 3% powdered milk and 0.05% Tween 20 (Thermo Fisher Scientific). Protein detection was performed using the anti-EBOV GP (ZGP 12/1.1 ...
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bioRxiv - Microbiology 2022Quote: ... PCR products (1□μg) of desired templates were 3’ end-labeled using a Pierce biotin 3’ End DNA Labeling Kit (Thermo Scientific). The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA) ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were sonicated for 10 min in 100% HPLC EtOH (J.T baker 8462-25) and then incubated for 24 min in a mixture of 3% 3-Aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC EtOH ...
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bioRxiv - Immunology 2023Quote: ... viable CD45-CD34+Sca-I- cells were sorted from epidermal single cell suspensions of 1-month-old EGFRΔEgr2 (n=3) and littermate controls (n=3) into Trizol LS Reagent (Thermo Fisher Scientific) using a FACS Aria III ...
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bioRxiv - Microbiology 2023Quote: ... King’s B (KB) medium was prepared by mixing 20 g/L of proteose peptone #3 (BactoTM Proteose Peptone No. 3, ThermoFisher Scientific), 1.5 g/L of dipotassium phosphate (Carl Roth GmbH + Co ...
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bioRxiv - Microbiology 2023Quote: ... cells were blocked for 1 h in 3% BSA/PBS and incubated with rabbit-anti-GFP (1:50 in 3%BSA/PBS, 200 µl/well, Invitrogen, G10362) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... HuH6 hCLDN1 [13] and Lunet N#3 and Lunet N#3 hCD81 [18] were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Thermo Fischer Scientific) supplemented with 1 % MEM non-essential amino acids (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... and 643 bp downstream of 943rd nucleotide from 3’ end (3’ flank) of cysK2 were PCR amplified from Rv genomic DNA using Phusion DNA polymerase (Thermo Scientific). The amplicons were digested with BstAPI and ligated with compatible hygromycin resistance (hygr ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis (cleaved Caspase 3/7) measurements were done using the FMK-FLICA-Caspase 3/7 Kit (+ PI staining; Thermo Fisher Scientific) following the manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: RNAs were extracted from whole testes of 3 Ptbp1fl/fl and 3 Ptbp1ΔSC mice at postnatal day (P) 35 using the PureLink RNA Mini Kit (Ambion, Cat. 12183025). RNA-seq libraries were constructed using Illumina’s TruSeq Stranded mRNA sample prep kit ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... AF6-88.5.5.3, #17-5958-82), CD119 (1:100, 2E2, #13-1191-82), Armenian Hamster IgG isotype (1:100, eBio299Arm, #13488881) (Invitrogen); CD45 (1:400 ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were diluted to less than 1 ×106 cells/mL in PBS with 3% FBS and incubated in 1:100 EpCAM-APC (Invitrogen, 17-5791-80) or 1:100 IgG-APC isotype control (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with 100 ng/ml LPS for 3 hr followed by incubation with 2 µM Mitosox red (Thermo Fisher Scientific, M36008) for 30min ...
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bioRxiv - Molecular Biology 2019Quote: ... cell collection proceeded by adding TRI Reagent (Life Technologies, 3 mL per 15 cm dish), scraping the cells off the plate ...
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bioRxiv - Immunology 2019Quote: ... PBMC were stimulated as described above and Brefeldin A (3 µg/ml, Thermo Fisher Scientific) was added after the first 2h of stimulation before intracellular staining for CD40-L.
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bioRxiv - Cell Biology 2019Quote: ... The pellet was resuspended 3 times using 7 mL Wheaton Dounce tissue grinder (Fisher Scientific) and centrifuged at 12,000 rpm at 4°C for 30 minutes (Sorvall SS-34 centrifuge rotor) ...