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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The soluble fraction was then incubated 1h with HisPur Ni-NTA Resin (Thermofisher), and the proteins bound to the resin purified as described before ...
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bioRxiv - Bioengineering 2021Quote: ... was added for 1h and samples were fixed using ProLong Diamond (Thermo Fisher) for at least 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and for 1h with rabbit anti-goat alexa647 conjugated secondary antibody (Invitrogen A21446). Images were acquired using Zeiss axioimager Z1 microscope with DAPI ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1h incubation with HRP anti-rabbit conjugated secondary antibody (ThermoFisher; #31462) at room temperature under constant agitation ...
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bioRxiv - Neuroscience 2019Quote: ... using 1.5ug of DNA per 40K neurons for 1h in Neurobasal A (Invitrogen). After transfection ...
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bioRxiv - Cell Biology 2024Quote: ... blocked for 1h at RT in blocking solution (5% rabbit serum (10510, ThermoFisher) and 1% BSA (A4503 ...
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bioRxiv - Cell Biology 2021Quote: ... The insoluble fraction was removed by high-speed centrifugation at 4°C (25658 x g for 1 hour, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was incubated with 2 ml Ni-NTA resin (Thermo SCIENTIFIC #88222 ...
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bioRxiv - Immunology 2021Quote: ... mixed (1:1 in volume) with Fluo 4 dye (Fluo 4 Direct Calcium Assay Kit, ThermoFisher Scientific) and incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... pTG-Luc and SARS2-COV2 spike expression vector were transfected into HEK 293T cells at the ratio of 3:4:3 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific, Waltham, MA). Accession IDs of the spike proteins used in the study were ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were blocked in blocking buffer (1% BSA, PBST) for 1h at room temperature and incubated with primary antibody LC3A/B (Thermo Scientific, USA) overnight at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Washed samples were incubated for 1h at RT with the secondary antibody (1:200 Alexa Fluor 647 goat derived anti-rabbit antibody, Thermo Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2020Quote: ... for 2h at RT followed by a 1h incubation with 1:200 secondary goat anti-rabbit antibody Alexa 647 (Thermo Fisher, A32733) and DAPI to visualize cell nuclei ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were washed and incubated for 1h with Alexa Fluor 488-conjuated antibody to rabbit IgG (A-21206, 1:100, Thermo fisher scientific). Images of green fluorescence were assessed by laser confocal fluorescence microscopy.
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Overexpression of HLA class I predominantly on alpha cell in at risk individuals for type 1 diabetesbioRxiv - Immunology 2020Quote: ... for 1h at room temperature (RT) followed by detection using polyclonal goat anti-mouse IgG-Alexa Fluor 647 (Life Technologies, 1:1000) for 30 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted at 1/50 or 1/150 followed by an incubation for 1h at room temperature with a secondary goat Alexa FluorTM 488 antibody (ThermoFisher Scientific, #A11029) diluted at 1/1000 ...
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bioRxiv - Microbiology 2024Quote: ... then the following secondary antibodies were diluted 1:500 in blocking buffer and incubated for 1h with the cells: anti-rabbit DyLight 448 (Thermo Scientific, 35552), anti-rabbit DyLight 550 (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... the slides were incubated with primary antibodies in PBS containing 5% BSA for 2 h and then with the following secondary antibodies for 1h at room temperature: Donkey Anti-Rabbit Alexa 488 (1:1000; Invitrogen; A21206, 2376850) and Donkey Anti-Chicken Alexa 594 (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... stained with primary antibody for 1h at 37° C and labeled against F-actin (1:60 in PBS Alexa Fluor® 488 Phalloidin-Invitrogen, # A12379) and DNA (NucBlue ...
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bioRxiv - Cell Biology 2024Quote: ... incubated in blocking buffer supplemented with 1 µg/ml Hoechst 33342 for 1h at RT with secondary antibodies: Alexa Fluor 647 goat anti-mouse (Invitrogen, A-21236), Alexa Fluor 488 chicken anti-rabbit (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... 6% native PAGE gels were prepared by first making a gel solution containing 6% acrylamide (29:1) (Fisher Scientific).15 This solution was then degassed under vacuum and mixing ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Microbiology 2021Quote: ... 9 x 105 cells of each indicated SARS-CoV-2-non-infectable cell type were stained with CellTrace™ Violet (Thermo Fisher # C34557), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were first loaded for 3 min onto the Acclaim PepMap 100 C18 trap column (75 µm x 2 cm, 3 µm material, Thermo Scientific) with 5 µL min-1 of 3.2% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... Tetramethylrhodamine methyl ester (TMRM, cat n. T668, Molecular Probes, Leiden, The Netherlands) was dissolved in DMSO to obtain a 10 mM stock solution and then diluted in the appropriate buffer ...
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bioRxiv - Cell Biology 2020Quote: ... cells were co-stained with 16.7 nM tetramethylrhodamine methyl ester (TMRM; Invitrogen) and 100 nM Mitotracker Green (MTG ...
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bioRxiv - Microbiology 2021Quote: ... Cysteine alkylation was induced by addition of methyl methanethiolsulfonate (MMTS, Thermo Scientific) in 100% isopropanol to obtain a final concentration of 10 mM followed by a 10 min incubation in a thermoshaker at 37°C ...
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bioRxiv - Immunology 2021Quote: For measurement of mitochondrial membrane potential TMRM (Tetramethylrhodamine, Methyl Ester, Perchlorate; ThermoFisher) was added to the cells at 30 nM final concentration ...
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bioRxiv - Molecular Biology 2023Quote: To assess paraquat (Methyl viologen 98%, Thermo Fisher Scientific Cat. No.: 227320050) toxicity ...
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bioRxiv - Developmental Biology 2022Quote: ... using donor embryos labeled with tetramethyl rhodamine methyl ester (TMRM, Fisher Scientific). To produce contemporaneous stage-matched embryos for PMC transplants ...
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bioRxiv - Genetics 2023Quote: ... 1X cutting solution: 93 mM N-Methyl-D-glucamine (Acros Organics, AC126841000), 2.5 mM KCl (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... proteins were eluted with a 1M α-Methyl-D-mannopyranoside (Acros Organics) pH8 solution.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 10.6 mM Nα-tosyl-L-arginine methyl ester HCl (Fisher Scientific, T03301G), 10.8 mM tosyl-lysine-chloromethylketone HCl (Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... with single-cell passaging every 2-3 days using accutase (ThermoFisher Scientific A1110501) at split ratios between 1:5 and 1:12.
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bioRxiv - Plant Biology 2020Quote: ... from 2-3 weeks old seedlings and 500ng was treated with DNaseI (Ambion) and used for reverse transcription ...
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bioRxiv - Neuroscience 2021Quote: ... TRPA1−/− male mice were loaded with Fura-2 (3 μg/mL, Life Technologies) and pluronic acid (1:1 ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 2 mM caspase-3/7 detection reagent (Fisher Scientific) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... were all purchased from Sigma and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium was purchased from Thermofisher.
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bioRxiv - Biochemistry 2020Quote: ... and cells were loaded with 3 μM Fura-2 AM dye (Molecular Probes) in sterile-filtered HEPES-Tyrode Buffer (HTB ...
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bioRxiv - Cell Biology 2021Quote: ... Primary cells were loaded with Fura-2 AM (3 μM; Invitrogen/ThermoFisher Scientific) for 45 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Primary cells were loaded with Fura-2 AM (3 μM; Invitrogen/ThermoFisher Scientific) for 45 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... p97 siRNAs (2-HSS111263 and 3-HSS111264) were from Invitrogen (Thermo Fisher Scientific). p97 rescue constructs were previously published 53 and were resistant to siRNA # 2 ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 and Caco-2 cells were cultured in minimum essential medium (GIBCO) while A549-ACE2 cells were maintained in DMEM/F-12 medium (GIBCO) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP) (Thermo Fisher Scientific, USA) in PBS (Thermo Fisher Scientific ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) (M6494, Thermo Fisher Scientific) was added to each well to a final concentration of 0.67 mg/ml and incubated at 37oC ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP, Thermo Fisher Scientific, USA) in PBS (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... After 2-3 days cells were harvested with Trizol (Invitrogen, Carlsbad, CA, USA) for RNA-seq study.
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bioRxiv - Biochemistry 2019Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Invitrogen and dissolved in 1X PBS (pH 7.4 ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Cells were passaged every 2-3 days using 0.05% Trypsin-EDTA (Gibco, 25300062). These cells were cultured in Knockout™ D-MEM (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Capan-2 and BxPC-3 cell lines were cultured in RPMI medium (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Genomics 2024Quote: ... Cells were passaged every 2-3 days using 0.25% trypsin-EDTA (#25200056, Gibco) to keep them below 80% confluency ...