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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Primary cortical neurons were loaded with 2 µM Fluo-4 (Invitrogen) in KRH (Krebs’–Ringer’s–HEPES containing (in mM) ...
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bioRxiv - Neuroscience 2020Quote: ... Cultures were loaded with 2 µM Fluo-4 AM (Thermo Fisher) in Neurobasal-A media supplemented with B27 without phenol red for 30 minutes at 37°C in a 5.5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2019Quote: ... Then Fluo-4 AM (2 µM final concentration) (Thermo Fisher, F14201) was added directly to the medium and incubated for another 30 min at 37℃ ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2 µM Fluo-4 AM (Thermo Fisher Scientific, Waltham, USA). After a loading time of 45 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were incubated with 2 μM Fluo-4 AM (Invitrogen) diluted in HBSS solution (0.4 gL−1 KCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 mM MgCl2 and 2 µl of Platinum Taq polymerase (Invitrogen) with added water to a final volume of 25 µl ...
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bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2021Quote: ... Calcofluor white was purchased from Sigma-Aldrich and SYTO 9 was purchased from Thermo Fisher Scientific.
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bioRxiv - Biophysics 2022Quote: ... supplemented with 9% Fetal Bovine Serum (FBS, Gibco) and a penicillin/streptomycin mixture (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... using 9 uL of Lipofectamine RNAiMAX (Life Technologies). After 40 hours post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... SYTO-9 (ThermoFisher, #S34854; final concentration: 2.2 μM) was used ...
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bioRxiv - Developmental Biology 2022Quote: ... for mature miR-9 or SuperScript III (Invitrogen) with random hexamers for pri-miRNAs ...
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bioRxiv - Microbiology 2019Quote: ... syto-9 and propidium iodide (Life technologies(tm)) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The diluent was HMI-9 media (Invitrogen, UK). Blood stream form T ...
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bioRxiv - Cell Biology 2021Quote: ... a 7-9% agarose (Invitrogen, cat no. 16500500) pad made from the imaging media was gently placed over the top of the worms ...
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bioRxiv - Cancer Biology 2021Quote: ... UD cells were cultured in LHC-9 (Gibco) supplemented with 1% Pen/Strep ...
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bioRxiv - Cell Biology 2019Quote: ... caspase-9 (10 µM ZLEHD-R110; Molecular Probes), or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence ...
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bioRxiv - Bioengineering 2022Quote: SYTO® 9 (Thermo-Fisher Scientific, Waltham, MA) was used to fluorescently stain nucleic acid of the human oral microbiome pooled samples to investigate viability at 24 h in the anatomical gingival tissue model in comparison to the planar construct ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... supplemented with 9 % heat inactivated FBS (Gibco 10500064) at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... the epithelium was stained with Syto 9 (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... combined with 9 µl lipofectamine 2000 (Invitrogen 11668019). Transfection mixtures were incubated at room temperature for 15 mins ...
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bioRxiv - Biophysics 2023Quote: ... 0.8 µL crosslinker BS(PEG)9 (Thermo scientific) and 10 μM protein were incubated for 1 hour at 4℃ and filtered by Superose 6 in buffer (20 mM HEPES ...
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bioRxiv - Systems Biology 2019Quote: ... coli One Shot® ccdB Survival™ 2 T1R were used for propagation of pDONR207 (Invitrogen, UK) and destination vectors ...
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bioRxiv - Neuroscience 2020Quote: ... One μg of the construct was combined with 2 μL Lipofectamine 3000 (L3000-015, Thermo Fisher Scientific) and added to the cells ...
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bioRxiv - Systems Biology 2021Quote: ... This barcoded destination vector pool was transformed into One Shot ccdB Survival 2 T1R Competent Cells (Invitrogen) that were spread on 245 mm × 245 mm square LB+ampicillin plates and incubated overnight at 37°C for colony isolation ...
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bioRxiv - Microbiology 2021Quote: ... or 10 μM 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) succinimidyl ester (DDAO-SE) (Invitrogen) in HMI11 and incubated for 20 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: (D1)2 and iE5 concentrations were estimated by UV absorption using NanoDrop™ One/OneC (Thermo Scientific) (extinction coefficients ...
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bioRxiv - Genetics 2022Quote: ... All destination vectors were transformed with One Shot ccdB Survival 2 T1R Chemically Competent Cells (Invitrogen A10460) with both Ampicillin and Chloramphenicol selection.
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bioRxiv - Microbiology 2022Quote: ... 2 mL malt extract (2% w/w) in 4 mL screw neck vials (Fisher Scientific, Loughborough, UK) were inoculated with an agar piece of the G ...
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bioRxiv - Cell Biology 2024Quote: ... adipose tissue (2∼4 cm3) was washed twice with PBS solution (AQ) containing 2% penicillin-streptomycin (Invitrogen), and then cut to 1∼2 mm3 with scissors ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1-10 ng template in 9 μl Nuclease-free water were added to 10.0 μL of 2× TaqMan® Universal PCR master mix (ABI/Life technologies, USA) and 1.0 μL of a 20× combined primers and probes mix (ABI/Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... Bisulfite-PCR was conducted using primer sets (Integrated DNA Technologies; Table 1) designed against specific genomic regions with Methyl Primer Express v1.0 software (Thermofisher Scientific). PCR amplicons were cleaned with AmpureXP beads (#A63882 ...
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bioRxiv - Systems Biology 2021Quote: ... A total of 90 μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (Thermo Scientific, Lafayette, CO, U.S.A.) was then added to the samples and shaken at 1400 rpm for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured for 24 hours in culture medium supplemented with 0.5% (w/v) methyl cellulose and B-27 (1/50, Life technologies).
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bioRxiv - Neuroscience 2020Quote: ... Secondary neurospheres grown for 3 days (3-4 million cells) were harvested and crosslinked with 0.5% formaldehyde (Thermo Fisher, cat. no. 28908) in 0.1 M DPBS for 5 min at RT with soft agitation ...
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bioRxiv - Cell Biology 2021Quote: ... The βarr1/2 siRNA (5’-ACCUGCGCCUUCCGCUAUG-3’) and a scrambled siRNA (control, 5’-UGGUUUACAUGUCGACUAA-3’) (Dharmacon) were transfected by RNAimax (Invitrogen) according to the instructions of the manufacturer ...
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bioRxiv - Synthetic Biology 2024Quote: ... the full volume of media in each well was pipetted gently 4-5 times and added to 1 mL of FACS buffer containing 3 uM DAPI (PBS pH 7.4, 2–5 mM EDTA, 0.1% BSA, 3 uM DAPI (Thermo Scientific #62247)) ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 1 mL of the following mixture to the culture media and incubated the cells at 25°C incubator for 1—3 hours: 5 μM Fura-2 AM (F-1201, Life Technologies), 250 μM probenecid (162-26112 ...
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bioRxiv - Biophysics 2021Quote: α2-GFP or α2:β at 1:3 ratio was transfected into HEK293T cells on collagen coated glass bottom dishes using lipofectamine 3000 (Invitrogen) for expression of α2 GlyR or α2β GlyR ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...
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bioRxiv - Biochemistry 2022Quote: ... and then 1 μg of each peptide sample was loaded on a rap column (75 µm × 2 cm, 3 µm, C18, Thermo Fisher). The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transfer to a new tube ...
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bioRxiv - Molecular Biology 2022Quote: ... The grids were washed by dipping 2 times in PBS (37°C) before 3 μL of 1 μm Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and applied to plasma-cleaned CF-300 2/1 grids before being blotted for 3 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transferred to a new tube ...
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bioRxiv - Microbiology 2023Quote: ... The products were enzymatically cleaned (3 ul of PCR reaction, 2 ul water, 1 ul of ExoSap-IT (Applied Biosystems 78200)) for 15 minutes at 37°C followed by 15 minutes at 80°C ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 μg DNA) and β-arrestin-2-mCherry (1 μg DNA) using 3 μL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...