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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 6 Fluoro 1 3 benzodioxene 8 carbonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The RNA was then incubated with 5M sodium chloride and isopropanol and incubated with Dynabeads M-270 Streptavidin (Thermo Fisher, 65305) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Red blood cells were removed by incubating the blood samples with 2mL Ammonium-Chloride-Potassium Lysing Buffer (Thermo Fisher Scientific #A1049201) for 3 to 5minutes at RT ...
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bioRxiv - Microbiology 2023Quote: ... 2TY media components (tryptone, yeast extract, sodium chloride) and cation-adjusted Mueller Hinton Broth (CAMHB; Becton Dickinson) were purchased from Fisher Scientific.
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bioRxiv - Genomics 2023Quote: Whole ovaries were isolated using aseptic technique and placed in ice cold and sterile 1X Phosphate Buffered Saline (1X PBS, without calcium and magnesium chloride; Gibco 10010023). Blood and other contamination were carefully removed by washing the tissues with fresh 1X PBS ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions of spleens were prepared using 70-μm cell strainers and red blood cells lysed by incubation in Ammonium-Chloride-Potassium (ACK) Lysing Buffer (Life Technologies). Single-cell suspensions of lungs were prepared by digestion with 0.25mg/ml Collagenase IV and 15 μg/ml DNase for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... chloride hemipentahydrate (Cd2+), lead (II) nitrate (Pb2+), mercury (II) chloride (Hg2+), and hydrogen peroxide (H2O2, 30% in water) were purchased from Fisher Scientific. Buthionine sulfoximine (BSO) ...
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bioRxiv - Neuroscience 2023Quote: ... and whole media fractions were mixed with the fluorescent membrane dye Octadecyl Rhodamine B Chloride R18 (0.33 ng/ul, Life Technologies, O246) and incubated for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... (4) 30 min incubation in ammonium chloride (NH4Cl) and (5) 4 min incubation in Tissue Autofluorescence Quenching Kit (ReadyProbes, ThermoFisher Scientific).Secondary antibodies were used as follows ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into pCR™8/GW/TOPO® vector using a pCR™8/GW/TOPO® TA Cloning® Kit (Invitrogen) as described by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial suspensions were sonicated on ice (15s on, 45s off for 8 min total; amplitude 8 um; Fisher Scientific Model 705 Sonic Dismembrator). The samples were centrifuged at 10,000 x g for 1 min ...
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bioRxiv - Immunology 2021Quote: ... Interleukin-8 (IL-8 or CXCL8) protein in the basolateral media was measured using a magnetic bead-based ELISA (Procartaplex, ThermoFisher Scientific, Waltham, MA) according to the instructions provided by the manufacturer and the plate were then read using a Luminex-based Bio-plex Multiplex system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... two cecal slurry from challenged chicks raised on fresh litter and reused litter were spread plated onto CHROMagar™ plates supplemented with or without gentamicin (8 ppm) and tetracycline (8 ppm) or ampicillin (16 ppm) and cefoxitin (16 ppm) (Thermo Fisher, Waltham, MA). After 24 h incubation ...
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bioRxiv - Microbiology 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a Human IL-8 Uncoated ELISA kit (Invitrogen, ThermoFisher Scientific Inc.).
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bioRxiv - Zoology 2023Quote: ... Supernatants were collected 24 hours post-treatment and then IL-8 secretion was quantified using commercial human IL-8 ELISA kits (Invitrogen, cat. #88-8086) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cell Biology 2020Quote: ... Tissue was then transferred to 1% fibronectin-coated chambered slides (μ-Slide 8 Well Glass Bottom, Ibidi #80827, for live imaging; Nunc™ Lab-Tek™ II Chamber Slide with removable wells ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate volume was increased to 1 ml and 8 μg of antibody were used in combination with Protein G Dynabeads M-280 (Thermo Scientific) as described above ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested before the cultures reached OD600 = 1 and were labeled with 8 mg/ml EZ-Link Sulfo-NHS-LC-biotin (ThermoFisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
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bioRxiv - Cell Biology 2021Quote: ... and the pellets were dissolved in 0.1% SDS/ 0.67 M Tris-HCl pH 8 +/- 15 mM 4-acetamido-4’-maleimidylstilbene-2,2’-disulfonic acid (Thermo Fisher Scientific, USA). The samples were incubated at 37 °C for 2 h ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS (pH 8) and incubated with 1 mg/ml of EZ-link Sulfo-NHS-biotin (Thermo Fisher) in PBS (pH 8 ...
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bioRxiv - Genetics 2022Quote: ... or one of five Hsa21 genes (100 ng/well; 1 row, 8 wells or technical replicates per unique cDNA) using Lipofectamine 2000 (Invitrogen, 11668030) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... which were then blotted for approximately 8 s and immediately plunged in 1:2 ethane:propane (Carbagas) at liquid nitrogen temperature using a Vitrobot (Thermo Fisher Scientific). The Vitrobot chamber was kept at 4 °C and 100% humidity during the whole procedure.
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bioRxiv - Microbiology 2020Quote: ... The pH was adjusted to pH 8-8.5 with 1 M NaHCO3 and 50 µg of Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) was added ...
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bioRxiv - Microbiology 2021Quote: ... Solubilized membranes were mixed 1:1 with binding buffer (20 mM Tris pH 8/1 M NaCl/20 mM imidazole) and loaded onto a HisPur Ni-NTA (Thermo Scientific) column equilibrated with 20 mM Tris pH 8/750 mM sucrose/500 mM NaCl/10 mM imidazole/0.2% DDM (equilibration buffer) ...
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bioRxiv - Biochemistry 2020Quote: ... High-resolution mass spectra and MS/MS fragmentation pattern of 1-8 were recorded using a Q Exactive Plus mass spectrometer (Thermo Scientific). Chromatography for determination of amino acyl hydroxamate was performed on a Waters ACQUITY H-class UPLC system coupled to a Xevo TQ-S micro (Waters ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Immunology 2020Quote: ... was applied to the grids and blotted (Whatman #1 paper) for 2 to 8 s using Vitrobot Mark IV (Thermo Fisher) with a blot force of 5 ...
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bioRxiv - Cell Biology 2022Quote: ... and 4.8 µL cDNA (diluted 1:20 prior to use) was added to each well of qPCR plates (REF 4346906, Life Technologies). Samples were run on a StepOnePlus™ RT-qPCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: Total 40μg of protein from cell lysates were loaded in 8%SDS-PAGE gel and immunoblotted with RAD51 antibody (1:1000; PA5-27195; Thermofisher Scientific) diluted in 1%BSA+TBS+ 0.1%Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... Differentiated iNeurons were then cryogenized as aliquots of 1 · 106 to 8 · 106 cells in heat inactivated fetal bovine serum (FBS, 10270-106, Thermo Fisher) containing 5% v/v dimethyl sulfoxide (DMSO) ...
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bioRxiv - Neuroscience 2023Quote: ... were maintained in DMEM with 1g/L of D-glucose supplemented with 8% Fetal Bovine Serum/FBS and 1% Pen/Strip (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Imaging was done in 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA).
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bioRxiv - Genetics 2023Quote: ... IP and input DNA were purified using ChIP elution buffer (250 mM NaCl, 1% SDS, 10 mM Tris-Cl pH 8, 1mM EDTA) and incubation with proteinase K (ThermoFisher Scientific) overnight at 65°C ...
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bioRxiv - Genomics 2023Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Developmental Biology 2023Quote: ... Imaging was done in 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA).
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bioRxiv - Biochemistry 2023Quote: ... Freshly isolated mitochondria were resuspended in 50 μl of click-it lysis buffer (50 mM TRIS-HCl pH 8, 1% SDS, 250 U/mL Universal Nuclease [Thermo Fisher]) and incubated on ice for 15 min ...
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bioRxiv - Immunology 2024Quote: ... cleanup beads were used (0.8:1 beads/sample ratio) and then eluted in 10 µL of DNase/RNase-Free Distilled Water (10977- 049, ThermoFisher®). cDNA was quantified by the Quant-iT PicoGreen dsDNA Assay Kit (P7581 ...
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bioRxiv - Immunology 2019Quote: ... 5’-CCCTACTGTATCCTCATG-3’/5’-CTTACCTCCTCTTCAATAGC-3’ PRKDC: 5’-GGGGCATTTCCGGGTCCGGG-3’/5’-TGCCCTGCCCCCCACTCTGC-3’ Amplicons were cloned using the Zero Blunt TOPO PCR Cloning kit (ThermoFisher), prepared as plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... Guide RNA primer sets A (KS40 5’-ACCGACCACAGAAACTGCGACTAG -3’ and KS41 5’-AACCTAGTCGCAGTTTCTGTGGTC-3’) and B (KS42 5’-CCGTCCCACTGGCACCGCTTTATG-3’ and KS43 5’-AAACATAAAGCGGTGCCAGTGGGA-3’) were phosphorylated with T4 polynucleotide kinase (ThermoFisher) at 37°C for 30 min and annealed by cooling down from 85°C to 25°C at 0.1°C/sec ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were washed 3 times in PBS followed by incubation for 1 hour at RT with AlexaFluor-conjugated secondary antibodies (1:400, Molecular Probes) and 0.15% DAPI (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... samples were washed with washing buffer (0.2%Tween-20/10mg·L-1Heparin/ PBS) for 1 day with 3 times refresh and incubated with dye-conjugated secondary antibodies (1:500, A-21245, Thermo Fisher) in antibody incubation buffer for 1 week at 37 °C ...
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bioRxiv - Genomics 2019Quote: ... were harvested and placed in 3:1 ethanol:glacial acetic acid fixative and then transferred to fresh 3:1 fixative containing 1% Polyvinyl Pyrrolidone (BP431-100, Fisher Scientific, USA). Emerging anthers from the flower buds were isolated and squashed under a 22 × 22 mm glass cover-slip to provide the best anthers for pachytene analysis ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS ...
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bioRxiv - Microbiology 2021Quote: ... the monolayers were overlaid with 3 mL containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were dry-mounted on slides using Fluoromount (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and were transferred into tubes containing 3 μl of RNase inhibitor solution (1 part 20 U μl−1 RNase Inhibitor [Thermo Fisher Scientific] in 19 parts 0.2% Triton X-100 ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... A pre-warmed buffer containing DPBS supplemented with 10% FBS and CellEvent Caspase 3/7 Green (1 μL/1 mL of buffer; ThermoFisher C10427) were added to the cells ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).