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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for 6 Fluoro 1 3 benzodioxene 8 carbonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
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bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from freshly-dissected n=3 young (3-month-old) or n=3 aged (18-month-old) zebrafish brain in TRIzol (Invitrogen, 15596). Three independent experimental replicates were used for bulk RNA sequencing ...
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bioRxiv - Immunology 2021Quote: ... After 3 consecutive washes anti-rabbit Alexa Fluor-568 conjugated antibodies (1:500; Thermo Fisher Scientific) were applied for 2 h before counterstaining with DAPI.
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bioRxiv - Microbiology 2020Quote: ... Parasitemias were determined on day 3 by staining with SYBR Green 1 nucleic acid stain (Invitrogen) at 1:2000 dilution in PBS/0.3% BSA for 20 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... slices were further incubated with TO-PRO-3 Iodide (diluted in PBS, 1:2000, Life Technologies) at RT for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... then 500 μL volumes of blood were diluted in sterile PBS (1:3) (BR0014G, Thermofisher Scientific) and centrifuged at 500xg for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... at a ratio of 1:3 of DNA: PEI in 200 μl of opti-MEM (Invitrogen) (Wang ...
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bioRxiv - Neuroscience 2020Quote: ... or 3) Goat anti-rabbit conjugated with Alexa Fluor 647 (1:1000) (Life technologies, Carlsbad, CA) for 2 hours at room temperature protected from light ...
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bioRxiv - Cell Biology 2020Quote: ... 3-NP-induced cytotoxicity was measured by fluorescence using the die YOYO-1 (#Y3601, Life Technologies). Treated cells were placed in an Incucyte FLR imaging system and the YOYO-1 fluorescence was measured after several time points ...
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bioRxiv - Bioengineering 2022Quote: ... in a ratio of 1:3 with a supplement of H2O2 (30%, Certified ACS, Fisher Scientific) at 200 °C for 50 min in a microwave digestion system (Titan MPS ...
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bioRxiv - Microbiology 2021Quote: ... were mixed at a ratio of 1 µg DNA : 3 µLTransit-T293 in Opti-MEM (Gibco). For an experiment conducted in triplicate at a 48-well scale ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were allowed to adhere for 1-3 hours before media was changed to Neurobasal (Invitrogen) supplemented with 1x B27 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: RNA from 1-3 x 106 cells was extracted and purified with TRIzol reagent (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 2016) are presented in Table 1 using an Applied Quant Studio 3 (Applied Biosystems, CA, USA). For PKoV RNA and PAstV-1 RNA detection ...
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bioRxiv - Microbiology 2020Quote: O/N bacterial cultures were diluted to an OD600 of 0.15 in TSB with 1 µg/mL 1,1′-didodecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC12) (Invitrogen). Cultures were grown to an OD600 of 1.0 ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 2 weeks of selection with 3 μg mL−1 of puromycin (Thermo Fisher Scientific). Then ...
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bioRxiv - Developmental Biology 2021Quote: ... and the nuclei were stained with TO-PRO®-3 1:1000 (Thermo Fisher Scientific, USA) for 10–20 min ...
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bioRxiv - Microbiology 2021Quote: ... at a 1:3 ratio (mg DNA/mg PEI) mixed in Opti-MEM (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... The sections were counterstained with TO-PRO™-3 (T3605, Invitrogen, OR, USA; dilution 1:1000) and examined using a confocal laser microscope (DMi8 ...
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bioRxiv - Genetics 2019Quote: ... except that 1-Bromo-3-chloropropane was used instead of chloroform and DNAse (TURBO DNase, Ambion) treatment was carried out ...
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bioRxiv - Neuroscience 2022Quote: ... psPAX2 and pMD2.G with a ratio of 4:3:1 in Opti-MEM (Gibco, 31985070) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control ...
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bioRxiv - Cell Biology 2022Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Cell Biology 2022Quote: ... cells were plated and transfected with 1:3 ratio (RNA:Lipofectamine) using Lipofectamine RNAiMAX reagent (ThermoFisher Scientific) following manufacturer recommendations ...
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bioRxiv - Genomics 2022Quote: ... with 1 ml per 4 million cells of 3 mM disuccinimidyl glutarate (DSG) (ThermoFisher Scientific, 20593) for 40 min at room temperature and quenched by 0.4 M Glycine for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Plant Biology 2022Quote: The stock solutions for the GLVs (Z)-3-hexen-1-ol (Z3-HOL, 98%; Acros Organics) and (Z)-3-hexenyl acetate (Z3-HAC ...
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bioRxiv - Immunology 2024Quote: ... and a 3:1 CD3/CD28 mouse T-activator Dynabead to Treg ratio (Thermo Fisher Scientific). On day 3 of expansion ...
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bioRxiv - Cell Biology 2024Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Cell Biology 2023Quote: PASMCs were stained with Hoechst 33342 dye (3 μL/ml of 1:100 dilution; 62249; Invitrogen) for 15-30 min at 37° C to visualize apoptotic cells ...
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bioRxiv - Microbiology 2023Quote: ... 3% sucrose) diluted 1:4 in Leibovitz’s L-15 medium without phenol red (Gibco, Waltham, MA) and an adjusted osmolality of 340 mOsm using 1 M sucrose ...
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bioRxiv - Cell Biology 2023Quote: ... with PBS (3 times) and incubated with a secondary antibody (1:500, Molecular Probes AF488 #A11008) and Hoechst33342 (1:2000 ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... 1 mM L-cysteine) with papain (3 U/mL) and DNAse I (150 U/μL, Invitrogen) for digestion during 25 min at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 µL of template cDNA using a QuantStudio 3 real-tame PCR system (Applied Biosystems). PP2AA3 was used for normalization ...
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bioRxiv - Cancer Biology 2023Quote: ... 3% agarose gels were prepared with a 1:20000 dilution of SYBRSafe DNA gel stain (Invitrogen). Once complete the PCR products were loaded onto the gel and the electrophoresis was run on a Powerpac Electrophoresis system (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then washed and incubated with a 1:1000 dilution of To-Pro-3 (ThermoFisher) in PBS for nuclear staining and then mounted using VectaShield antifade mounting medium (Vector Labs ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% P/S at a cell density between 3 × 105 and 2.5 × 106 cells/ml (GIBCO). Cells were always handled and maintained under sterile conditions (tissue culture hood ...
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bioRxiv - Genetics 2023Quote: ... the nuclei were stained by applying 3 mL of 1:1000 diluted DAPI (Thermo Scientific, 62248) in 2×SSC in 4 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then mounted using culture medium supplemented with 1 μM TO-PRO-3 (Life Technologies) for nucleic acid staining ...
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bioRxiv - Microbiology 2023Quote: O/N bacterial cultures were diluted to an OD600 of 0.15 in TSB with 1 μg/mL 1,1′-didodecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC12) (Invitrogen). Cultures were grown to an OD600 of 1.0 ...
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bioRxiv - Developmental Biology 2024Quote: ... zebrafish were incubated for 3 h in 1 µM LysoTracker™ Deep Red (Invitrogen™, L12492) in E3 (DMSO) ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-occludin (1:100 in 3% BSA in TBS; Thermo Fisher Scientific, Cat# 71-1500) overnight at 4°C in a humidified atmosphere ...
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bioRxiv - Biochemistry 2024Quote: ... 1-3 nmol of mixed antigen was bound to pre-equilibrated Dynabeads MyOne Streptavidin C1 (ThermoFisher). The purified phages were mixed with the beads pre-loaded with a combination of hCNPase and mCNPase and incubated for 2 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... The library was subsequently cloned into the plasmid pCR™8/GW/TOPO™using the pCR™8/GW/TOPO™ TA Cloning Kit (Thermofisher).
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bioRxiv - Plant Biology 2021Quote: ... The cells were then washed in PBS and placed individually on the cap of an 8-tube PCR strip (Axygen Low Profile 8-Strip PCR Tubes; Fisher Scientific 14-223-505). The presence of a single meiotic cell without attached debris was confirmed microscopically during both pick-up and release (10X magnification ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were pelleted at 1,200 RPM for 5 minutes then resuspended in 500 μl of the complete mouse keratinocyte–serum-free medium without calcium chloride (Gibco Cat# 37010022). Cell number and viability were measured by Automated Cell Count (Invitrogen Countess II FL ...
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bioRxiv - Microbiology 2021Quote: ... Major ions (ammonium, sodium, potassium, calcium, magnesium, chloride, nitrate and sulphate) were determined using Ion Chromatography DIONX ICS-1100 (Thermo Scientific, USA). Total nitrogen and total organic carbon (TOC ...
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bioRxiv - Neuroscience 2022Quote: Live-cell imaging to show mature neuronal response to potassium chloride (KCl) or acetylcholine (ACh) was performed using Fluo-4-AM (Invitrogen™, F14217). Cells were incubated with Fluo-4-AM in HBSS-Ca2+ (45 min ...