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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 6 Chloro 7 fluoro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... 50 μg of pre-aliquoted Tandem Mass Tag 6-plex (TMT-6, Thermo Scientific) was resuspended in 20 μL anhydrous acetonitrile ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma IL-6 was measured by immunoassay (ProQuantum Human IL-6 Immunoassay Kit, ThermoFisher) and run on the ABI 7300 (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: FsC[Fe3+] was labelled by 6-carboxyfluorescein (6-FAM) (Thermo Fisher Scientific, Waltham, USA) to indicate the targeting of FsC[Fe3+] to C ...
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bioRxiv - Molecular Biology 2019Quote: ... This was followed by a 1h incubation at room temperature with 25μl Pierce protein A Magnetic Beads (Thermo Scientific). The beads were washed and resuspended then either prepared for Western blotting or added to Qiazol lysis reagent for RNA extraction ...
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bioRxiv - Biophysics 2021Quote: ... HsTMEM120A was subjected to protease digestion with 1mg/ml proteinase K at 37°C for 1h (Thermo Scientific; EO0491). 0.2% SDS was added to the digestion solution to stimulate the activity of proteinase K ...
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bioRxiv - Neuroscience 2022Quote: ... following 1h incubation at RT with corresponding Alexa 488/Cy3/Cy5 coupled secondary antibodies (1:1000, Thermo Fisher Scientific). Cell nuclei were stained with DAPI ...
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bioRxiv - Microbiology 2022Quote: ... and incubated for 1h at RT with AlexaFluor488-conjugated donkey anti-mouse IgG secondary antibody (0.2 µg/ml; ThermoFisher) diluted PBS-HS ...
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bioRxiv - Biophysics 2022Quote: ... Samples were incubated for 1h with a filtered and sonicated solution of red fluorescent carboxylate-modified beads (FluoSpheres, Invitrogen) of 100nm (220nm filter ...
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bioRxiv - Cell Biology 2019Quote: ... Detection was performed with a 1h incubation at RT with secondary antibodies AlexaFluor488 or AlexaFluor594 (1:500; Molecular Probes), and DAPI (1:500 ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... Teased fiber preparations were permeabilized in 0.25% TX-PBS for 30 min and blocked for 1h in 1% NGS/1% horse serum (Gibco)/1% HEPES/PBS ...
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bioRxiv - Neuroscience 2020Quote: ... the strips were incubated with goat anti-human IgG Fc HRP-conjugated secondary antibody for 1h (Thermo Fisher Scientific). The film was exposed following routine immunoblotting steps ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed for 1h at RT to secondary antibodies: Goat anti-chicken AlexaFluor 488 (1:1000, ThermoFisher, A21131), Donkey anti-rabbit AlexaFluor 555 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... for 1h at room temperature with gentle mixing followed by inactivation with 40U of SUPERase-In RNase inhibitor (Ambion). Cell extracts were passed through Sephacryl S400 spin columns (GE ...
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bioRxiv - Neuroscience 2021Quote: ... which were then blocked during 1h at RT with Superblock™ in PBS blocking buffer (Thermo fisher Scientific #37515) or PBS containing 0.1% Tween 20 and 3% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... Cell were incubated at 37°C for 1h in thyroid organoid media with mitotracker green 1:1000 (Invitrogen, M7514) and mitosox red 1:500 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... eBiosciences) for 1h before sections were mounted in 0.1 M phosphate buffer and cover-slipped with Flouromount G (Invitrogen) mounting media ...
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bioRxiv - Physiology 2022Quote: ... Hearts were again washed trice in PBT (0.2% Triton X-100 in PBS) for 1h each and incubated in blocking solution (10% FBS (4133, Invitrogen), 1% BSA (A7030-10G ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed three times within PBS and incubated for 1h at room temperature with the secondary antibody (Alexa-488 anti-mouse and anti-rabbit IgG, 1:2,000, Invitrogen; CY3 anti-rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... Electrophoresis was carried out in MOPS buffer by applying a constant current of 50mA for 1h (Electrophoresis kit, Invitrogen). Tubulin concentrations were assessed either by using Coomassie staining or acetylated tubulin content ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed twice in PBS-T for 5min and incubated for 1h with anti-rabbit AlexaFluor555 (Invitrogen, #A32794) or anti-rabbit AlexaFluor647 (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... corresponding secondary antibodies were added for 1h at RT: (Alexa-594-conjugated goat anti-rabbit IgG (H+L) (Invitrogen), Alexa-594-conjugated goat anti-chicken IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 times with TBS-T and incubated 1h at RT with an HRP-conjugated anti-mouse secondary antibody (dilution 1:50,000, G-21040, Invitrogen). After 3 washes in TBS-T ...
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bioRxiv - Physiology 2024Quote: ... and incubated for 1h at RT with a chicken anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies, Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... eBiosciences) for 1h before sections were mounted in 0.1 M phosphat buffer and cover-slipped with Flouromount G (Invitrogen) mounting media ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibodies were diluted according to the manufacturer’s instructions and incubated for 1h at RT (488-ms/rb; 555-ms/rt; 647-ms, 1:1000, Invitrogen). Nuclei were visualized using 0,1μg/ml DAPI (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products detected on 6% polyacrylamide or 2% agarose gels were purified with a Charge Switch PCR Clean-Up kit (Invitrogen, Carlsbad, CA) and then sent to BMR genomics (Padova ...
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bioRxiv - Developmental Biology 2020Quote: ... 2×105 cells/well (6-well plate) HCECs were transfected with 30pmol siRNA using lipofectamine RNAiMax (Life Technologies, Grand Island, NY, USA) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... DAB-developed samples were stored in 70% glycerol and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, #D3571, 1:1000) nuclear marker ...
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bioRxiv - Immunology 2022Quote: ... containing 4′,6-diamidino- 2-phenylindole (DAPI) and were transferred onto microscope slides with large-orifice 200 µL-tips (Fisher Scientific, 02707134). The following antibodies or reagents were used ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were then washed as above and incubated in 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) at RT for 10 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DAPI (4-6-diamidino-2-phenylindole) and DyLight 488-or DyLight 555-labeled secondary antibodies (1:400, Thermo Fisher Scientific, Waltham, MA) were added for 1.5h at room temperature ...
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bioRxiv - Genomics 2019Quote: ... We incubated this mixture at 72 °C for 3 min for denaturing followed by a quick chill on ice and adding the remaining 6 µl reverse transcription mix that contained 2 µl of 5x First Strand Buffer (Thermo Fisher Scientific), 0.1 µl of 100 µM SMARTer II oligo (5’-AAGCAGTGGTATCAACGCAGAGTACATrGrG+G where “+” indicates a locked nucleic acid (LNA ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were subsequently rinsed in PBS and counterstained with 1:5000 dilution of DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride, Invitrogen, Eugene, OR) in deionized water for 3-5 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Biochemistry 2020Quote: ... one well of a 6 well plate was transfected with 2 μg of plasmid DNA with Lipofectamine® 2000 (Life Technologies™) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed three times with PBS and mounted on the slide with ProLong Diamond antifade with 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies, #P36966). Confocal images were acquired on a Nikon Ti-Eclipse A1M microscope fitted with a 60× oil immersion objective using 488 nm ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were subsequently plated on islands of glial cells at a density of 1.25 × 104 cells/cm2 in 6-well plates in Neurobasal-A supplemented with 2% B27 and 0.2% penicillin/streptomycin (Invitrogen/ Thermo Fisher, Waltham, MA). Lentiviral particles encoding synaptoPAC were added 1–4 days after plating ...
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bioRxiv - Bioengineering 2022Quote: ... D13+14 PT-enhanced organoids (triplicate wells per condition) were incubated in 4’,6-diamindino-2-phenylindole substrate (DAPI; 1:1000 [Thermo Fisher Scientific]) with 1:500 DRAQ7 dead cell label (Thermo Fisher Scientific] ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were washed three times and ProLong® Gold Antifade mounting reagent containing DAPI (4’,6-diamidino-2-phenylindole) (Thermo Scientific, #P10144) was applied to mount the coverslips.
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bioRxiv - Microbiology 2021Quote: ... then 50 μl of the EV preparations were mixed with 250 μl PBS staining buffer containing 2.5 µg/ml DAPI (4’, 6-diamidino-2-phenylindole; Thermo Fisher Scientific, USA)) and kept at 4 °C for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... The cells were again washed and incubated for 10 min in a solution of 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific). Cells were imaged using a Leica DM IRB epifluorescence microscope ...
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bioRxiv - Developmental Biology 2019Quote: ... aliquots of 5 × 104 293FT cells were seeded onto poly-L-lysine (PLL)-coated 6-cm dishes and co-transfected with 2 μg of each pCAG-based expression vector using Lipofectamine 3000 (Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for overnight with primary antibody at 4 degrees and further incubated with secondary antibodies (1:500) for 1 hour followed by 4′,6-diamidino-2-phenylindole (DAPI) or phalloidin 488 (1:400, Thermo Fisher, A12379) staining ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... sections were rinsed three times for 10 min each and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Immunology 2020Quote: ... Isolated B cells were stained for 20 minutes on ice with a fluorescence staining-mix containing 4’,6-Diamidin-2-phenylindol (DAPI; Thermo Fisher Scientific), anti-human CD20-Alexa Fluor 700 (BD) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa-Fluor 594 labelled goat anti-rabbit IgG, ProLong Gold antifade reagent with DAPI (4’, 6-diamidino-2-phenylindole) (Life technologies-Invitrogen, USA), ATP Affinity kit (no ...
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bioRxiv - Neuroscience 2021Quote: ... One million pre-differentiated cells were plated to each well of a Nunclon 6-well dish with 2 ml of the high purity differentiation media: DMEM/F12 (Thermo Fisher 21331020) containing high purity N2 (above) ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclei were counterstained with DAPI (4’,6-diamidino-2-phenylindole) and filamentous actin with Alexa Fluor 488-conjugated phalloidin (Thermo Fisher Scientific).
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bioRxiv - Genetics 2022Quote: ... These pieces were transferred to the lower surface of a 25 cm2 culture flask (6-8 pieces per flask) which had been pre-moistened with 1-2 mL of AmnioMAX Complete Medium (Thermo Scientific, #11269016) supplemented with 1% penicillin/streptomycin (GenDepot ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled with DAPI together with the secondary antibody (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10.000, Thermo Fisher Scientific, #D1306). The following secondary antibodies were used ...