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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for 6 Chloro 7 fluoro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 1×106 cells per condition were plated in 96 U-shape well plates (CELLSTAR, Kremsmünster) and rested for 2-3 h in RPMI (Gibco), supplemented with 10% FBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... and cell viability was then determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay (Invitrogen, USA) according to the manufacturer’s protocol 28 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction solution was vortexed for 2-3 seconds to avoid bubbles and added to 384-well plate (Nunc™ MicroWell™ 384-Well Optical-Bottom Plates ...
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bioRxiv - Cell Biology 2020Quote: ... 2×106 NP-TTD were transfected with 3 µg plasmid via electroporation using Neon™ Transfection System (MPK1096, Invitrogen) with following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... Activated/Cleaved Caspase-3 (1:1000; Cell Signalling Biology, CAT#: 9661S and Thermo Fisher Scientific, CAT#: 66470-2-IG), Nestin (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: Fresh tissue samples were washed 2–3 times in PBS and incubated in 5 U/ml dispase (ThermoFisher Scientific) supplemented with antibiotics (penicillin 50U/I and streptomycin 50 mg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Neuroscience 2023Quote: Cellular viability was determined utilizing a MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Invitrogen, Waltham MA), as described previously (Kumar et al. ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 3’ end RNA was synthesized by in vitro transcription using an mMESSAGE mMACHINE T7 kit (Invitrogen) with a DNA template (5’- TAATACGACTCACTATAGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAAUGACGTAGCATGCTACGCG -3’ ...
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bioRxiv - Neuroscience 2022Quote: ... We reverse-transcribed ∼2-3 µg DNase treated RNA into cDNA using Superscript III Reverse Transcriptase (Invitrogen no. 18080093). For each reaction ...
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bioRxiv - Neuroscience 2024Quote: ... The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Cell Biology 2024Quote: ... equipped with a trapping column (3 µm C18 particle, 2 cm length, 75 µm ID, Acclaim PepMap, Thermo Scientific) and a 50 cm long separation column (2 µm C18 particle ...
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bioRxiv - Genomics 2023Quote: ... and 100 μg/ml cycloheximide using Beckman Coulter UC tubes 9/16 x 3-1/2 (Fisher Scientific, NC9194790), and equilibrating overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... coupled to an Acclaim PepMap 100 column (2 cm x 75 μm, 3 μm particle size, Thermo Fisher Scientific) coupled in line with an EASY-Spray PepMap RSLC C18 reversed-phase column (50 cm x 75 μm ...
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bioRxiv - Microbiology 2023Quote: ... tilt series were acquired at binning 2 on a Falcon 3 camera under the controls of Tomography software (ThermoFisher). For cryo-ET of the enrichment culture ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc ...
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bioRxiv - Cancer Biology 2023Quote: Mouse primary myoblasts were isolated from limb muscles of pups (2-3 days old) and cultured in Ham’s F-10 nutrient mixture (Invitrogen) with 20% FBS (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were synthesized from 2 to 3 μg of total RNA using SuperScript IV and Oligo dT12-18 (Thermofisher) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... followed by seeding of 2-3×106 cells onto T25 flasks (Thermo-Fisher Scientific; Cat. No. 12-556-009) coated with Matrigel ...
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bioRxiv - Cell Biology 2023Quote: Mouse primary myoblasts were isolated from the limb muscles of 2-3 day-old pups and cultured in Ham’s F-10 nutrient mixture (Invitrogen) as described before.38 The culture medium was supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: ... The peptides were first concentrated on a precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and then separated on an EASY-Spray column (ES903 ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 2 µL streptavidin-HRP (1:5,000; S911, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were maintained in a water jacketed 5 % CO2 incubator and passaged every 2-3 days using trypsin/EDTA (Gibco). 1E6 cells were seeded into 6 well plates ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 7 days with secondaries (Goat anti-rabbit Alexa-568, Invitrogen A11036, or Goat anti-Rabbit Alexa-647, Invitrogen A-21245 1:500, 2 ml / sample) at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 5-(and-6)-carboxyfluorescein (FAM) and 5-(and-6)-carboxytetramethylrhodamine (TAMRA) were obtained from Invitrogen™ ...
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bioRxiv - Plant Biology 2023Quote: ... 6 uL of 0.5 M EGTA (bioWORLD) and 6 uL of 0.5 M EDTA (Invitrogen) were added to quench the reaction at 65 °C for 10min ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted and purified from synovial tissue (a total of 7 samples from 7 different RA patients) and cultured cells using Trizol reagent (Life Technologies, Carlsbad, CA, USA), followed by cDNA synthesis using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara-Bio ...
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bioRxiv - Microbiology 2023Quote: ... niveus CO7 DNA ranging from 7 to 7 × 10−4 ng/µl was generated and quantified using a Nanodrop-8000 Spectrophotometer (Thermo Scientific, Wilmington DE, USA) (260/280 ratio of 1.89) ...
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bioRxiv - Biophysics 2021Quote: ... The ligation was desalted by dialysis using membrane filters for 1h and later concentrated 3.3X using a SpeedVac concentrator (Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... and the cells were serum-starved for 1h by adding 1.5mL Opti-MEM® reduced-serum medium (Gibco, Life Technologies) per well prior to transfection ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed 3 times with washing buffer (1x PBS and 0.025% of Tween20) for 5 min and incubated for 1h at RT with the Alexa Fluor secondary antibodies (Thermo Scientific). Cells were washed twice with washing buffer for 5 min and incubated in with DAPI (1 μg/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... Dissociated murine prostate cells were obtained by digesting pre-minced prostate tissue for 1h at 37°C in digestive medium prepared in prepared in ADMEM/F12 (Gibco), and containing 1mg/mL Collagenase Type I (ThermoFischer Scientific ...
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bioRxiv - Molecular Biology 2021Quote: J1 mES cells were grown on glass coverslips and incubated for 1h (37°C, 5% CO2) with EU from the Click-iT RNA Alexa Fluor 594 imaging kit (C10330, Invitrogen). Cells were fixed with 3,7% formaldehyde in PBS 1× for 15min at room temperature and permeabilized with 0.5% Triton X-100 in PBS 1× for 15min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... W4021) were added for 1h and protein signals were detected using Pierce™ ECL Western Blotting Substrate kit (ThermoFisher 32109), using a Fujifilm LAS4000 luminescence imager and band intensities quantified using ImageJ ...
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bioRxiv - Cell Biology 2019Quote: ... Permeabilized cells were then incubated with anti-megalin and anti-FLAG antibodies at 4°C for 1h in the presence of 0.05% saponin followed by incubation with Alexa 488 or Alexa 647 secondary antibodies (Molecular Probes) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... burnetii (1:2500; Robert Heinzen, NIH, Hamilton, MT) for 1h followed by Alexa fluor secondary antibodies (1:1000; Life Technologies) for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... Blocking was done in 3% BSA in PBST 0.5% for 1h at room temperature followed by EdU detection with the Click-iT EdU reaction kit (Thermo Scientific), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... slides were rinsed with PBS and incubated for 1h with secondary antibodies (Alexa Fluor 488 anti-rabbit and Alexa Fluor 568 anti-rat; Invitrogen) and DAPI (4083 ...
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bioRxiv - Microbiology 2020Quote: ... The non-encapsidated DNA was then removed by a 1h incubation at 37°C with 4 U TURBO DNase enzyme (Invitrogen), 1X TURBO DNase Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... washed thrice with PBST followed by 1h incubation with Alexa fluor conjugated secondary antibody (Thermo Fisher Scientific Cat # 11008, 21244) then again washed thrice with 1XPBST ...
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bioRxiv - Immunology 2022Quote: ... were generated by plastic adherence for 1h in complete RPMI 1640 (680 μM L-Glutamine and 10 mM Hepes, GIBCO) supplemented with 5% pooled human serum (The Blood Bank ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 1h in goat-anti-rabbit AlexaFluor 488 secondary antibody (1:200; #A11034, ThermoFisher Scientific, MA), followed by 5x PBS washes ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated at 25°C for 1h in a solution containing 0.1% Triton X and Hoechst 33342 (1:5000, Invitrogen, H3570). Sections were mounted with Fluoromount-G (Southern Biotech ...
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bioRxiv - Cell Biology 2021Quote: ... samples were incubated at room temperature for 1h with the corresponding fluorescence secondary antibodies conjugated to Alexa Fluor 488 or 555 (Invitrogen) at 1:400 ...
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bioRxiv - Bioengineering 2021Quote: ... Chips were then incubated at 37°C for ~1h until cells were fully dissociated into single cells at which point DTI (Gibco) was added to neutralize the enzymatic activity ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... were coated for 1h with 0.1% gelatin solution (ATCC, PCS-999-027) and washed twice with PBS (pH 7.4, Thermo Fisher Scientific). HEK293FT cells were plated on the gelatin coated dish and grown overnight in normal growth medium to reach ∼ 70% confluency ...
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bioRxiv - Physiology 2019Quote: ... Primary antibody was detected by a 1h incubation with AlexaFluor-conjugated goat anti-rabbit secondary antibody (Life Technologies, A-11072) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated for 1h with a secondary antibody: Alexa-488nm donkey anti-rat or goat anti-mouse or goat anti-rabbit (Invitrogen) (dilution 1/400) ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed in TBST buffer prior to incubation with secondary fluorophore conjugated antibodies 1:2000 for 1h at RT (goat anti-mouse DyLight 680 Invitrogen A21058 ...
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bioRxiv - Microbiology 2020Quote: ... 0.05% saponin at room temperature for 1h followed by Alexa FluorTM 647 goat anti-rabbit IgG (ThermoFisher A21245; 1:1000), 0.05% saponin at room temperature for 1h followed by nuclear staining with Hoechst 33342 (Thermo Fisher Scientific 62249 ...