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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Fixed monola were blocked with 4% BSA-PBST and probed with primary antibodies for pSTAT1-Tyr701 (ThermoFisher Scientific MA515071, 1:250 dilution) and Chlamydia heat-shock protein 60 (cHsp60 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked with 4% horse serum in PBS for 1 hour and then incubated with primary antibodies against cTnT (Thermo Fisher Scientific), α-actinin (Abcam) ...
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bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
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bioRxiv - Microbiology 2024Quote: ... and 4 μl was spotted onto LB agar or on LB agar with 1% (w/v) sodium deoxycholate (DOC, Thermo Fisher Scientific), as has been previously described (56) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 10 min with PBS and incubated over-night at +4°C with goat anti-rabbit Alexa Fluor 564 (1:1000, Invitrogen, ThermoFisher Scientific) and goat anti-mouse Alexa Fluor 488 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 7 min with PBS and incubated overnight at +4°C with goat anti-rabbit Alexa Fluor 594 and goat anti-mouse Alexa Fluor 488 (each 1:1000, Invitrogen, ThermoFisher Scientific) in carrier solution ...
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bioRxiv - Neuroscience 2024Quote: ... 1h at RT and subsequently incubated overnight at 4°C in primary antibody solution using Rabbit anti-Green-Fluorescent-Protein (GFP; 1:1000 ThermoFisher Scientific, A6455). The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides were resuspended in 10 µL of water and labeled for 1 h at room temperature with 4 µL (∼11 µg/µL in acetonitrile) of TMT10plex or TMT18plex (Thermo Fisher Scientific). The reaction was quenched with 4 µL of 5% hydroxylamine ...
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bioRxiv - Neuroscience 2024Quote: ... Cellular RNAs captured by bead-bound antibody complexes were digested to <100 nt by incubating for 30 min at 4°C with RNase I (1 U/μl; Thermo Fisher Scientific Ambion; AM2294). After extensive washing ...
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bioRxiv - Neuroscience 2024Quote: ... the slices containing the fixed neurons cross-linked with 4% paraformaldehyde and stained with streptavidin Alexa Fluor 555 conjugate (Invitrogen, 1:1000). Fluorescent images were obtained using a confocal microscope (ZEISS LSM 900 ...
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bioRxiv - Cell Biology 2023Quote: ... cells at 70-80% confluency were switched to myogenic differentiation medium (DMEM, 4% horse serum, 2 mM Glutamax, 1 mM sodium pyruvate (Life Technologies #11360070), 1 μg/mL insulin (GeminiBio #800122 ...
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bioRxiv - Biophysics 2023Quote: ... with a FuGene: DNA ratio of 3:1 using 4 μg of DNA in OptiMEM Reduced Serum Medium (ThermoFisher Scientific Catalog #11058021). The transfection medium was removed from cells ∼6–8 h post transfection ...
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bioRxiv - Biochemistry 2024Quote: ... HBMEC were incubated overnight at 4°C with the primary antibodies (anti-β-catenin, # 71-2700; anti-ZO-1, # 61-7300; anti-CAT1, # PA5-82800, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed 3x 10 min with PBS and incubated either for 2 hrs at RT or 24-72 hrs at 4° C in goat anti-rabbit Alexa Fluor 488 (1:1000, Invitrogen, ThermoFisher Scientific) secondary antibody in carrier solution containing 1% NGS ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated over-night at +4°C with donkey anti-sheep secondary antibody conjugated with Alexa Fluor 568 (1:1000, A-11011, Invitrogen, ThermoFisher Scientific) and Streptavidin conjugated with Alexa Fluor 488 (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with 5% non-fat dry milk overnight at 4°C under gentle agitation followed by 1,5 hours incubation at room temperature with anti-CRM1 (MA5-27879, Invitrogen, 1:2000 dilution) antibody to detect PmXPO1 protein ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed in PBS three times for 5 min at 4 °C by rocking and then incubated by rocking with secondary antibody (Goat-anti-Mouse 488, 1:2000, Invitrogen #A-11008) in 1% BSA PBST for 1 hour at RT in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... The grid was blotted for 3s (blot force 1) at 100 % humidity and 4°C in a Vitrobot Mark IV (Thermo Fisher Scientific) before plunge-freezing in liquid ethane.
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bioRxiv - Cell Biology 2023Quote: ... 5 µl polar metabolite extract were injected in push partial mode using an overfill factor of 1 onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific), additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Neuroscience 2023Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-GFP monoclonal antibody (1:500, G10362, Thermo Fisher Scientific) i n PBS containing 0.1% Triton X-100 and 1% normal goat serum for 2 days at 4°C ...
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bioRxiv - Genomics 2023Quote: ... 5 ul of cDNA reaction was used in each of 4 x 50 ul Round 1 PCRs per sample using Phusion polymerase in HF buffer (Life Technologies-F531L). The 4 Round 1 PCRs were then pooled and 5 ul of that mix were used as template for a 50 ul Round 2 PCR ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at 4°C before the addition of 25 μL of Pierce Protein A/G Magnetic Beads (Thermo Fisher Scientific) for an additional 2 h under constant rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates were incubated for overnight at 4°C with 2 μg of antibodies recognizing MTR4 or IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A (ThermoFisher Scientific). After incubation ...
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bioRxiv - Bioengineering 2023Quote: ... in PBS pH 7.4 or pH 6), HA (Sigma-Aldrich, 9067-32-7, 0.5%–1% w/v in Hanks’ Balanced Salt Solution (Gibco, 14025-050) or Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody incubation was also performed overnight at 4°C with donkey anti-mouse Alexa 647 (1:250, Invitrogen, Cat. # A-31571).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C in a primary antibody/0.5% BSA/PBS-T solution of rabbit anti-TH (1:1000, Invitrogen, Carlsbad, CA, 701949). Sections were then washed 6×10 minutes in PBS-T before incubating in secondary antibody/0.5% BSA/PBS-T solution of Alexa568 donkey anti-rabbit (1:2000 ...
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bioRxiv - Bioengineering 2022Quote: ... is purchased from MakingCosmetics Inc. (USA). 4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1M solution, Cat. No. J16924-AP) is purchased from Thermo Scientific (USA). Silica beads (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed with PBS and lysed at 4°C in ice-cold 1% triton lysis buffer supplemented with protease inhibitors (ThermoFisher Scientific, A32963) and phosphatase inhibitors (5 nM Na3VO4 ...
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bioRxiv - Cell Biology 2022Quote: ... Treated cells were incubated for 1 and 4 hours and total protein was isolated using RIPA lysis and extraction buffer (Thermo Scientific, 89900) and run using SDS-PAGE in 10% precast polyacrylamide gels (BioRad ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were then washed in PBS with 2% Triton X-100 at 37°C for 4 days and incubated with goat anti-rabbit Alexa 633 (A-21071, Invitrogen, 1:100) at 37°C for 5 days ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labeled with DAPI together with the secondary antibody (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10.000, Thermo Fisher Scientific, #D1306). The following secondary antibodies were used ...
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bioRxiv - Immunology 2022Quote: ... The leaflets were then incubated for 2 days in the dark at 4°C with goat anti-Rat IgG Cross-adsorbed Alexafluor 594 secondary antibody dilution 1:500 (#A11007, Thermo Fisher Scientific), washed 3 times in 1 mL PBS (pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: First-round PCR amplification of exon 4 was carried out on 1 μl of single worm lysate using Phusion Green High-Fidelity DNA Polymerase (Thermo Scientific, F534S) in a final volume of 20 μl as follows ...
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bioRxiv - Microbiology 2022Quote: ... proteins were separated on Bolt 4-12% Bis-Tris gel in 1 × Bolt MOPS SDS running buffer (Thermo Fisher Scientific catalog # B0001). Separated proteins were electro-transferred to nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were centrifuged at 300 x g for 5 min at 4°C and treated with 1 mL of Ammonium-Chloride-Potassium (ACK) Lysing Buffer (Gibco; cat#A1049201). After 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4 °C with the primary antibody rabbit anti-CaMKIIα polyclonal antibody (1:1000, Invitrogen PA5-84083), mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were processed for 48 hours at 4 degrees with the primary antibody in blocking solution (1:1250, rabbit anti-Esr1, Invitrogen PA1-309), rinsed 3×10 minutes in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... ATP measurements were taken before and after activation with magnetic Dynabeads coated with anti-CD3/CD28 antibodies (Thermofisher, 4:1 beads/cell).
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was cleared at 45’000⍰g for 25⍰min at 4⍰°C and the supernatant was loaded into HisPur NiNTA Chromatography 1 ml cartridge (Thermo Fisher Scientific). The resin was washed with 50 ml of buffer A containing 20⍰mM imidazole ...
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bioRxiv - Developmental Biology 2023Quote: ... mucus-like compounds were stained by incubation (overnight at 4°C) with AlexaFluor 647-labeled PNA (1:1000 in PBSt; Molecular Probes #L32460).
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies were incubated on slides in a the dark for 3 hours at room temperature or overnight at 4°C: GαM 488 (Invitrogen A21121, 1:1000), GαRb 546 (Invitrogen A11010 ...
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bioRxiv - Immunology 2023Quote: ... a liver burn injury of approximately 1 mm3 was made on which a droplet of pHrodo Red succinimidyl ester (4 μM; Thermo Fisher Scientific) was administered ...
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bioRxiv - Immunology 2023Quote: ... The sections were fixed for 1 hour with 4% paraformaldehyde (PFA) in Hank’s Balanced Salt Solution (HBSS, Gibco, Waltham, MA, pH 7.2) supplemented with 0.1% Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... the coverslips were washed and fixed with 4% paraformaldehyde in PBS for 10 min at room temperature then stained with Alexa Fluor 488 Phalloidin (1:500; Invitrogen cat# A12379) in blocking buffer for 20 min at room temperature and mounted in DuoLink PLA mounting medium with DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... were plated into 12-well plate at a density of 3-4 × 105 cells in 1 mL per well in 1x DMEM (Gibco Cat# 11995065) plus 10% FBS (Gibco Cat# 16000044 ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% non-fat milk for 1 hour at RT and incubated overnight at 4°C with primary antibody [Sfrp1 (Invitrogen, MA5-38193), Nr3c1 (Cell signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... The PVDF membrane was blocked for 1 h with 5% BSA in TBST buffer containing 0.1% Tween 20 followed by overnight incubation at 4 °C with primary antibody for: ERα (MA5-14104, dilution 1:400, Thermo Fisher Scientific) and PR (sc-7208 ...
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bioRxiv - Immunology 2024Quote: CD14-positive monocytes isolated from human PBMCs (1 x 106 cells/well) were incubated with 27D6 antibody or isotype-matched control antibody (4 μg/mL, IgM, Thermo Fisher Scientific) for 30 min at room temperature ...