Labshake search
Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... was carried out overnight at 4° C followed by secondary antibodies (conjugated with Alexa Fluor 488 or Alexa Fluor 647 (1:250; Molecular Probes, Invitrogen) at RT for 4-5 hours ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C in secondary antibody diluted 1:500 in block (donkey anti-rabbit Alexa 568, Life Technologies A10042). Sections were again washed in TBS ...
-
bioRxiv - Neuroscience 2021Quote: ... the membranes were probed with the following primary antibodies at 4°C overnight: mouse anti-EphA4 C-terminus (Invitrogen, USA; 1:1000), goat anti-EphA4 N-terminus (R&D Systems ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2008) at 4°C overnight and then incubated with Alexa Fluor 488-conjugated anti-rabbit antibody (1/800, Life Technologies, Oregon, USA). For in situ hybridization of the mouse Mesp1 and Mesp2 mRNA ...
-
bioRxiv - Cell Biology 2022Quote: ... Slides were then washed as above and incubated in 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) at RT for 10 min ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... DAPI (4-6-diamidino-2-phenylindole) and DyLight 488-or DyLight 555-labeled secondary antibodies (1:400, Thermo Fisher Scientific, Waltham, MA) were added for 1.5h at room temperature ...
-
bioRxiv - Genomics 2020Quote: ... at a dilution of 1:100 overnight at 4°C followed by donkey Alexa488-conjugated anti-rabbit secondary antibody (Thermo Fisher Scientific) at a dilution of 1:200 for 30min at room temperature ...
-
bioRxiv - Genomics 2020Quote: ... and Alexa-Fluor® conjugated secondary antibodies (1:1000 dilution for 4 hours, anti-rabbit Alexa-Fluor®-647, ThermoFisher Z25308; anti-mouse Alexa-Fluor®-647, ThermoFisher Z25008 ...
-
bioRxiv - Genomics 2020Quote: ... Key Resources Table) and Alexa-Fluor® conjugated secondary antibodies (1:1000 dilution for 4 hours, anti-rabbit Alexa-Fluor®-647, ThermoFisher Z25308 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies (Supplemental Table 1) were incubated at 4°C overnight and secondary antibodies (Alexa Fluo 488, 555, or 647; Thermo Fisher Scientific) were incubated at room temperature for 1 hour ...
-
bioRxiv - Immunology 2021Quote: ... Xela cell lines were sub-cultured every 3 – 4 days at a 1:4 split and cultured at 26 °C in plug-seal tissue culture treated flasks (BioLite; Thermo Fisher Scientific) containing amphibian-adjusted Leibovitz’s L-15 (AL-15 ...
-
bioRxiv - Neuroscience 2020Quote: Lysates of worms were prepared by sonication in buffer consisting of 4% urea and 0.5% SDS with 1 x Halt™ Protease and Phosphatase inhibitor Cocktail (Thermo Fisher Scientific). HEK293 cell lysates were prepared using RIPA buffer consisting of 150 mM NaCl ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cDNA synthesis reaction was diluted 1:50 and 4 μL was used for a 10 μL qPCR reaction with PowerUp™ SYBR™ Green Master Mix (ThermoFisher) and primers specific for the reporter mRNA (Table S5 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Slides were subsequently rinsed in PBS and counterstained with 1:5000 dilution of DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride, Invitrogen, Eugene, OR) in deionized water for 3-5 min at room temperature ...
-
bioRxiv - Biochemistry 2021Quote: ... One microgram of peptides in a volume of 1-4 µL was loaded onto the Acclaim µ-Precolumn (0.5 mm х 3 mm, 5 µm particle size, Thermo Scientific) at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2020Quote: ... Membranes were incubated overnight at 4°C with 1:2000 dilution of primary antibodies (FLAG; Sigma, F7425 and HA; Invitrogen, 71-5500) followed by incubation with 1:5000 dilution of HRP-coupled secondary antibodies for 2h at room temperature and detected using enhanced chemiluminescence (Pierce ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with rabbit anti-PSD95 antiserum (1:1,000; cat# 51-6900, Invitrogen/ThermoFisher, Waltham, MA) and guinea pig anti-vGlut2 antiserum (1:12,000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with the rabbit anti-wnt8b primary antibody (EMELCA Bioscience) diluted 1:200 at 4°C for 48 hours in Pierce Immunostain Enhancer (Thermo Fisher Scientific). Sections were washed three times with TBS and incubated with Goat anti-Rabbit-biotinylated ...
-
bioRxiv - Neuroscience 2021Quote: ... hMOs were then washed overnight with 1X PBS at 4°C and incubated with Hoechst 33342 solution in 1X PBS for 2 h (1:1000, Thermo Fisher Scientific) and washed in PBS for 2 h ...
-
bioRxiv - Neuroscience 2020Quote: ... Brains were incubated with secondary antibody for 3 nights at 4°C with secondary antibodies at 1:500: goat anti-rabbit Alexa Fluor 488 (ThermoFisher #A-11008) and goat anti-mouse Alexa Fluor 647 (ThermoFisher #A-21236) ...
-
bioRxiv - Neuroscience 2020Quote: ... The culture medium was refreshed every day and cells were passaged every 4-5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2021Quote: ... 48 hours after transfection cells were splitted in a ratio of 1:4 and 300 μg/ml Hygromycin B (Thermo Fisher Scientific) was added to the cell culture medium for antibiotic selection of stably transfected cells ...
-
bioRxiv - Physiology 2020Quote: ... containing 1.5 ml of chilled (4°C) transfer media composed of Ham’s F-10 medium including 1 mM L-glutamine (LG; Thermo Fisher Scientific, Denmark), 0.1% heat inactivated fetal bovine serum (hiFBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was incubated for 1 hr at 4°C with 200 µL of pre-washed magnetic Dynabeads M-270 Epoxy (Thermo Fisher Scientific) conjugated to rabbit IgG (Sigma) ...
-
bioRxiv - Molecular Biology 2020Quote: ... each of the libraries was diluted 4-fold prior to running on a 1% Agarose E-gel (Life Technologies, #G402001, Carlsbad, CA) with a E-Gel 1-Kb Plus DNA Ladder (Life Technologies ...
-
bioRxiv - Developmental Biology 2020Quote: ... mucus-like compounds in Xenopus were stained by incubation (overnight at 4°C) with AlexaFluor 647-labeled PNA (1:1000 in PBST; Molecular Probes #L32460). Detailed protocol was published in (Walentek ...
-
bioRxiv - Microbiology 2020Quote: ... Two hundred microliters of 2 μM calcein AM/4 μM ethidium homodimer-1 in PBS (Live/Dead® Viability Kit, Invitrogen, USA) were added to the wells and plates incubated for 30 min in the dark ...
-
bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with 5% (w/v) skimmed milk overnight at 4°C and probed with anti-DsbA (1:1000, Thermo Fisher Scientific) or anti-AP (1:5000 ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were washed in TBS containing 0.2% Triton X-100 three times and incubated in Alexa 488-conjugated goat anti-rabbit secondary antibody (1:500, 4 hours; Invitrogen, A-11034) containing bisbenzimide (1 μg/ml) ...
-
bioRxiv - Biophysics 2021Quote: ... we diluted the provided solution 1:100000 and added 4 μL of the dilution to a coverslip (#1.5, Thermo Scientific Menzel Gläser). We then used a second coverslip used on top of the first one to have homogeneous distribution of beads on the field of view ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were induced with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 4 hours and harvested by centrifugation (Sorvall LYNX 6000, ThermoFisher Scientific) at 8,000 rpm for 10 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... The tissue was washed in warm PBS and incubated in pre-warmed Buffer 1 (PBS 93%, 0.5M EDTA 3%, RNA secure 4%, Thermo Fisher Scientific, Australia), at 37°C on a shaker for 10 minutes ...
-
bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C followed by washing and incubation with alexa-conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-3 hour in the dark ...
-
bioRxiv - Cancer Biology 2022Quote: ... Sections were washed 3 times for 10 min in PBS 1X + 0.1% Triton X-100 + 1% BSA and incubated for 6h with secondary antibodies at 4°C: goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 568 (1/500, Invitrogen #A-11031), goat anti-rabbit IgG (H+L ...
-
bioRxiv - Bioengineering 2022Quote: ... D13+14 PT-enhanced organoids (triplicate wells per condition) were incubated in 4’,6-diamindino-2-phenylindole substrate (DAPI; 1:1000 [Thermo Fisher Scientific]) with 1:500 DRAQ7 dead cell label (Thermo Fisher Scientific] ...
-
bioRxiv - Biochemistry 2022Quote: ... the supernatant was incubated at 4°C for 1 h with anti-BmAgo3 antibody23 conjugated to Dynabeads protein G (Thermo Fisher Scientific). The superparamagnetic beads were washed five times with wash buffer (30 mM HEPES-KOH ...
-
bioRxiv - Neuroscience 2022Quote: ... They were stained for 2 days at 4°C with a mouse-derived antibody against GFP (1:400, mAB 3E6, Invitrogen, Darmstadt, Germany), washed for 2 hours at RT in 1x PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cell counts are re-determined by using the Countess ll FL and adjusted to ~1000□cells/μl by low speed centrifugation at 4□°C and re-suspended in 1× PBS without calcium or magnesium (Thermo Fisher MT21040CV) supplemented with 0.04% BSA to prepare cells for emulsification ...
-
bioRxiv - Biochemistry 2022Quote: ... The MTS were stained with a mixture of 2 μM calcein AM and 4 μM ethidium homodimer-1 in PBS (Thermo Fisher Scientific) to stain for live and dead cells ...
-
bioRxiv - Microbiology 2022Quote: ... GTX135357) primary antibody for 16 h at 4 °C and detected with anti-rabbit-Alexa488 (1:200, A11008, Invitrogen, Carlsbad, CA, USA) secondary antibodies for 40 min at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were denatured for 1 minute at 70°C and RNA-protein complexes were resolved on NuPAGE 4-12% Bis-Tris Gels (Thermo Fisher, #WG1402A) at 180V for 1 hour ...
-
Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... cells were washed three times with ice-cold PBS and then incubated for 30 min at 4°C in PBS containing 1 mg/ml EZ-Link-sulfo-NHS-LC-LC-Biotin (Thermo Fisher Scientific). Next ...
-
bioRxiv - Immunology 2020Quote: ... Approximately 5 mg of purified ACE2 PD was covalently attached per 1 mL of 4% beaded agarose by amine coupling using AminoLink Plus resin (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2021Quote: ... The cells were washed twice and then reacted for 30 min at 4°C with Streptavadin-Cy5 (Thermo Fisher; 1:1000 dilution) or anti-mouse IgG-Alexa488 ...
-
bioRxiv - Microbiology 2021Quote: One-tenth of the protein samples and their corresponding inputs (pre-diluted 1/10) were size-separated on a bolt 4-12% Bis-Tris plus (Thermo Fisher Scientific) and stained with the silver stain kit (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were collected by spinning at 1000g for 5 min at 4°C and cell pellets were resuspended in 1 mL of Trizol (Thermo Fisher Scientific). Total RNA was extracted according to manufacturer instructions ...
-
bioRxiv - Microbiology 2021Quote: ... microsphere mixtures were successively incubated with serum samples (1:400) biotinylated protein A and biotinylated protein G (4 μg/ml each) (Thermo Fisher Scientific), and Streptavidin-R-Phycoerythrin (4 μg/ml ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were washed with ice-cold PBS-BSA before secondary labeling for 1 h at 4°C in 3 mL1:200 PE-conjugated streptavidin (Thermo Fisher S866) to label for bound ACE2 ...
-
bioRxiv - Cell Biology 2021Quote: ... blocked for 20 min at room temperature and stained overnight at 4 °C using an anti-V5 antibody (1/500; Invitrogen, Cat R96025) or anti-FLAG antibody (1:100 ...
-
bioRxiv - Bioengineering 2020Quote: ... samples were fixed with 4% paraformaldehyde (w/v) in PBS for 1 h and stained with Actin Red 555 (Molecular Probes, ThermoFisher) for 1h ...