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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The full Delta-Omicron recombinant spike was cloned into pcDNA6 (Invitrogen). Point mutations were introduced by overlap extension PCR ...
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bioRxiv - Cell Biology 2023Quote: ... 1:6,000,000 dilution of ERCC RNA spike-in mix (Ambion – 4456740), 1 mM dNTPs (NEB - N0446S) ...
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bioRxiv - Molecular Biology 2023Quote: ... and we added 2μl of 1x LP PFP spikes (Thermo Fisher). Detailed descriptions of the spike-in controls can be found in the exRNAQC study (Consortium et al. ...
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bioRxiv - Genomics 2023Quote: ... with the following exception: ERCC spike-ins (ThermoFisher Scientific, Waltham, MA) were added to the RNA after the extraction procedure ...
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bioRxiv - Genetics 2023Quote: ... The External RNA Controls Consortium spike-in (ERCC, Ambion, Austin, TX) was added to the samples to allow for normalization in accordance with the core facility’s standard operating procedure but was not used in our downstream analyses ...
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bioRxiv - Microbiology 2023Quote: ... codon-optimized spike expression plasmid using Lipofectamine 2000 (Invitrogen™ 11668019) following manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... An ERCC RNA Spike-In Mix kit (ThermoFisher Scientific cat. 4456740) was used to normalize total RNA prior to library preparation ...
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bioRxiv - Immunology 2019Quote: ... whole stomachs were finely minced in disassociation buffer (2% FCS in Hank’s Balanced Salt Solution without calcium and magnesium (Life Technologies) and 5 mM EDTA ...
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bioRxiv - Immunology 2021Quote: Released tissue cells were stained in aliquots of 1 ×106 cells/100 μL of PBS with 2% fetal calf serum (FCS, GIBCO) and 0.1% NaN3 for 30min on ice ...
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bioRxiv - Immunology 2021Quote: ... NK cells were then expanded for 6 days with hIL-15 50ng/ml (Miltenyi #130-095-765) in RPMI 20% FCS supplemented with L-glutamine (2 mM; Gibco), penicillin/streptomycin (100 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 4° C following by re-suspending the cells in 0.5-1 of ml cell isolation media (2% FCS, DPBS:HBSS=1:1 and 1mM SYTOX Blue Dead Cell stain (ThermoFisher Scientific). Cells were imaged before FACS and after FACS to confirm successful purification of GFP+ cells.
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bioRxiv - Immunology 2020Quote: ... gradient centrifugation and resuspended at 1-2 x 106 cells/ml in RPMI-10% FCS (RPMI 1640 w/L-glutamine (Cellgro Gibco) supplemented with 10% FCS (Cellgro Gibco) ...
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bioRxiv - Immunology 2021Quote: ... mice were directly perfused with PBS/ 2 % FCS/ 1 mM EDTA and liver digest medium (ThermoFisher Scientific/ Gibco, Waltham, Massachusetts) through the vena cava ...
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bioRxiv - Microbiology 2021Quote: ... The plate was incubated for approximately 5 min before the reaction was stopped with the addition of 2 M H2SO4 and the A450 was measured (Multiskan FC, ThermoFisher). A standard curve was established using serial dilutions of lysate from PA11 ...
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bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Content of two ampules containing the cell culture was resuspended in Hank’s solution supplemented with 2% FCS (fetal calf serum) (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... After two washes of the tissue with plating medium (DMEM containing 10% FCS, 2 mM glutamine, 50 mg/ml penicillin/streptomycin, Invitrogen), it was triturated in plating medium and subsequently cells counted ...
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bioRxiv - Immunology 2024Quote: ... Enzymes were quenched with PBS with 2mM EDTA and 2% FCS on ice and then red blood cells were lysed with room-temperature ACK lysis buffer (Gibco) and passed through a 100μm cell strainer.
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bioRxiv - Genetics 2023Quote: ... resuspended in 800 μL of PBS supplemented with 2 % FCS and analyzed on Attune NxT Flow Cytometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ASCs (FVS780-CD20-CD3-CD14-CD16-CD19+CD27hiCD38hi) were sort-purified from human peripheral blood mononuclear cells into culture media (10% FCS, 2 mM L-glutamine (Gibco), 100 units/mL Penicillin and 100 ng/mL Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were washed with PBS and resuspended in 200 µl buffer (PBS supplemented with 2% FCS) and analyzed via Attune NxT Flow Cytometer (ThermoFisher). For LIVE/DEAD analysis all cells were gated and viable cells were quantified as indicated in Figure 4 via FlowJo (Version 10.9.0 ...
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bioRxiv - Bioengineering 2023Quote: ... cells in each well were firstly washed with flow cytometry staining buffer: 3% FCS with 2 mM EDTA (15575020; Invitrogen) in PBS− ...
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bioRxiv - Immunology 2024Quote: ... and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS) and 100 U/ml of rhIL-2 (Gibco). 8×106 CTLs were transfected with 6 µg of plasmid DNA of each construct with P3 Primary Cell 4D-Nucleofector X Kit (Lonza) ...
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bioRxiv - Cancer Biology 2024Quote: ... Collagen plugs were set at 37°C/5%CO2 for 10 minutes, and then fibroblast growth medium was added (DMEM medium supplemented with 10% FCS, 2 mM L-glutamine (ThermoFisher) and 100U/mL penicillin/streptomycin (ThermoFisher)) ...
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of TgTRPPL-2-smHA protein was performed using the Pierce HA Tag/Co-IP Kit (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: The primary antibodies were as follows: mouse anti-HA tag (2-2.2.14) antibody (1:1000; Thermo Fisher Scientific, cat. no. 26183); rabbit anti-HA tag (C29F4 ...
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bioRxiv - Genetics 2021Quote: ... The lysate was cleared by centrifugation and incubated for 2 hours at 4°C with Tag specific magnetic beads (Dynabeads™ M-280 Streptavidin/Invitrogen for immunoprecipitation of Avitag-ZFP462 and Avitag-GLP ...
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bioRxiv - Molecular Biology 2021Quote: ... Gene fragments encoding multiple (2 to 9) HA-tags were designed in silico and ordered using GeneArt Gene Synthesis service from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... 20% fetal calf serum (FCS) (Invitrogen), 0.1 mM non-essential amino acids ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% FCS (Life Technologies) and kept on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... both supplemented with 10% FCS (Invitrogen), 2 mM glutamine (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... 10% FCS and HEPES 15mM (Gibco). For inhibitor/activator treatments samples were dissected at E11.5 or E11.75 ...
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bioRxiv - Neuroscience 2020Quote: ... After addition of 20% FCS (Invitrogen), the tissue was mechanically dissociated and filtered through a 35µm nylon strainer (Falcon) ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% FCS (Gibco®). Cells (2.5×104/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% FCS (Gibco®) and 1% penicillin/streptomycin (Gibco® ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% FCS (Gibco #10437028) and 50U/mL penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10 % dialyzed FCS (Gibco), 201.3 μM Met (63-68-3 ...
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bioRxiv - Molecular Biology 2022Quote: ... 10% fetal calf serum (FCS, Invitrogen) and 1% penicillin/streptomycin (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and supplemented with 10% FCS (Gibco) (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... with 10% heat-inactivated FCS (Gibco) and penicillin/streptomycin (Gibco) ...
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bioRxiv - Genetics 2022Quote: ... 15% FCS (Cat. #10500064, Life Technologies); 20 µl PHA-L (Cat.# 00-4977-03 ...
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bioRxiv - Cancer Biology 2022Quote: ... 15% fetal calf serum (FCS, Invitrogen), 450 μM monothioglycerol ...
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bioRxiv - Microbiology 2022Quote: ... and NPM1 (FC-61991, Life Technologies). Secondary antibody (A11029 or A11036 ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... and 10% heat-inactivated FCS (Invitrogen)) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... (hi-FCS, Gibco, Life Technologies, UK), were incubated (37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% FCS (Life technologies). All media were supplemented with 2 mM L-glutamine ...