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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for Recombinant Mouse Mcpt1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... All recombinant proteins were examined by SDS-PAGE along with Pierce BSA mass standards (Thermo Fisher), and their concentrations were calculated from infrared scanning of Coomassie-stained gels (Odyssey Fc Imaging System ...
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bioRxiv - Physiology 2024Quote: ... or 0.75 UI human recombinant insulin/kg body weight for ITTs (catalog no. 12585014, Gibco™). Tail glucose measurements were then taken at the indicated time points in the figures to measure glucose clearance.
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bioRxiv - Biochemistry 2024Quote: ... The recombinant baculovirus was prepared using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific). Spodoptera frugiperda Sf9 insect cells (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: Recombinant GCase N370S mutant was produced by using 293T Freestyle cells (Thermo Fisher Cat. No. R79007) grown in suspension in 293 Freestyle medium (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Dicer/TRBP enzyme was pre-mixed with 80 U RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), 5× dicing buffer (120 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... beads were incubated in kinase reaction buffer supplemented with 100 ng recombinant CAMK2D (Invitrogen, cat. PV3373), 100 µM recombinant calmodulin (Enzo ...
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bioRxiv - Molecular Biology 2024Quote: ... recombinant Cas9 protein (100 ng/μl; GeneArt Platinum Cas9 Nuclease, Thermo Fisher Scientific, Waltham, MA, USA), and ssODNs (400 ng/μl ...
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bioRxiv - Neuroscience 2024Quote: iPSCs from all lines were cultured in StemFlexTM medium on human recombinant vitronectin (Thermo Fisher Scientific) as described (34,35,37) ...
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bioRxiv - Immunology 2024Quote: ... recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) (300U/mL; Thermo Fisher Scientific, Waltham, MA) and recombinant human interleukin 4 (rhIL-4 ...
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bioRxiv - Genetics 2023Quote: ... and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen) for the PCR step using D388 specific primers 5′-GAGAGAAGGATTAGATTGTG-3′ and 5′-CCTGTATAATAGAAGTACCA-3′ ...
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bioRxiv - Immunology 2023Quote: ... The concentrations of the purified recombinant proteins were determined by the BCA protein assay kit (ThermoFisher) and separated by reducing SDS-PAGE and visualized by silver staining ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The concentrations of the purified recombinant proteins were determined by BCA protein assay kit (Thermo Scientific) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guideline 66 ...
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bioRxiv - Neuroscience 2022Quote: ... and the recombinant proteins were purified using HisPur Cobalt Superflow Agarose (Thermo Scientific, Waltham, MA, USA), dialyzed against 1× phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant tau was labeled using an Alexa Fluor 647 Protein Labeling Kit (Cat No. A20173, Invitrogen) with 500 mg of recombinant protein input at a concentration of 1 mg/mL according to the manufacturer’s recommendations ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 12 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) (Thermo Fisher Scientific, USA) was included in the culture medium ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Biophysics 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RBD-hFc proteins were captured by Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), washed by wash buffer (100 mM Tris/HCl ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: Recombinant PrPAP1Xa was secretively expressed using the Bac-to-Bac™ Baculovirus Expression System (Invitrogen, USA) as previously described22 ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: Recombinant Omicron RBD proteins from variants BA.2 and BA.4.5 were obtained from Fisher Scientific. To determine the specificity of mAbs ...
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bioRxiv - Biochemistry 2023Quote: The recombinant baculovirus was prepared using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific). For expression ...
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bioRxiv - Cancer Biology 2023Quote: ... containing 150 ng/µl sgRNA and 500 ng/ul recombinant TrueCut Cas9 protein (Thermo Fisher, A36496) using Invitrogen Neon Electroporator with 2 pulses of 1200 V ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant Bd37 and Venus (control) proteins were purified by using ProBond Purification system (Thermo Fisher Scientific) according to the manufacture’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... the recombinant section of the hisC::’ND5i’ allele was amplified by PCR using DreamTaq (Thermo Scientific) and primers hisC-ins-f/hisC-ins-r (Table 2) ...
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bioRxiv - Bioengineering 2024Quote: ... and 10 ng/mL recombinant Activin-A (338-AC, Bio-Techne) in RPMI 1640 (61870036, Gibco) with added B-27 supplemented minus insulin (12587010 ...
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bioRxiv - Biochemistry 2024Quote: The recombinant baculovirus was prepared using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific). For expression ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant proteins were purified under denaturing conditions (8 M urea) using Ni-NTA resin (Thermo Scientific). The purity of the recombinant proteins was assessed via SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... and containing recombinant human stem cell factor (h-SCF; Gibco, PeproTech, #300-07; 100 ng/mL) and human IL-6 (Gibco ...
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bioRxiv - Pathology 2024Quote: ... HT7 was the only anti-mouse antibody used and a mouse-on-mouse blocking agent (Invitrogen: R37621) was applied for 30 minutes post protein block washes to reduce noise from secondary antibody binding and an additional three 5-minute washes were added before primary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IgM-HRP and anti-mouse IgA-HRP (ThermoFisher), goat anti-mouse Igκ ...
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bioRxiv - Plant Biology 2024Quote: Mouse liver microsomes (purchased from Thermo: GIBCO Mouse (CD-1) Microsomes ...
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bioRxiv - Cancer Biology 2022Quote: ... Mm00440280_g1 (mouse, ThermoFisher), and TaqMan™ Universal Master Mix II ...
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bioRxiv - Genomics 2019Quote: ... α-mouse (Invitrogen #A31570), α-goat (Invitrogen #A21432) ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse laminin (Gibco) was then added and incubated at 37°C for 4 hours ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... mouse (Affymetrix, 902931), performed by Cnkingbio Biotech Co. ...
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bioRxiv - Cell Biology 2021Quote: ... GST (Mouse, Invitrogen), GM130 (Mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse (Invitrogen, A315570), and rabbit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... mouse (ThermoFisher, #23017015); Vitronectin ...
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bioRxiv - Cell Biology 2020Quote: HEK293T cells stably expressing a single copy of N-terminally tagged TTBK2- or GFP-BirA* were generated using Flp-IN T-REx cells (ThermoFisher K6500-01), Positive clones were selected with hygromycin resistance ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 WT cells and the UPF3 dKO clone 2 expressing either FLAG-tagged GST or UPF2 were labeled by culturing them for at least 5 passages in DMEM for SILAC medium (Thermo Fisher Scientific) supplemented with 9% FBS (Silantes) ...
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bioRxiv - Molecular Biology 2020Quote: HA-tagged ubiquitin (a gift of the F. Sicheri lab) was overexpressed in ES cells by transfection with Lipofectamine 2000 (Thermo Fisher Scientific), 500 ng per ∼8×106 cells in a 10 cm dish ...
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bioRxiv - Molecular Biology 2020Quote: ... the primary antibody was rinsed three times with PBS then Alexa Fluor 546-tagged goat anti-rabbit IgG secondary antibody (ThermoFisher, A-11035) was added at a 1:1250 dilution and incubated for 1 hour before a final 3x rinse with PBS.
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bioRxiv - Developmental Biology 2020Quote: ... The next day the antibody was washed five times in PBST 0.1% with gentle agitation at room temperature (30 minutes each) and fluorescently-tagged by horseradish peroxidase-catalysed signal amplification (Thermo Scientific, T20913)
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bioRxiv - Plant Biology 2020Quote: ... To create the 6xHis-tagged MBP fusion of full-length WUS the coding sequence was amplified with Phusion DNA Polymerase (Thermo Fisher Scientific), digested with NcoI/XhoI and ligated into pMG21053 previously digested with NcoI/SalI ...