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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Recombinant Mouse Mcpt1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and from the 3’-terminus by 10×His-tag nucleotide sequence and inserted into the pFastBac1 vector (Invitrogen, USA) via the BamHI(5’ ...
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bioRxiv - Developmental Biology 2022Quote: ... mCherry-Emp-V5His construct was cloned using Hifi DNA assembly kit (NEB E5520S into the pAc5.1/V5-His A vector (Invitrogen) using the following enzymes Acc65I and XhoI ...
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bioRxiv - Immunology 2023Quote: HCMV US18 and US20 were amplified from the HCMV TB40/E BAC (accession #EF999921) and subcloned into pcDNA3.1-V5/His (Invitrogen) via the KpnI/NotI sites to generate pcDNA3.1 US18-V5/His and pcDNA3.1 US20-V5/His ...
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were PCR amplified and ligated into the copper-inducible pMT/V5-His B vector (Invitrogen).
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bioRxiv - Biophysics 2024Quote: ... The cDNA was eluted from Dynabeads using 11 μL Formamide-ROX mix (1000 μl Hi-Di Formamide (Thermo Fisher), 8 μl of 350 ROX size standard (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... Amplification and cloning of the truncated versions of TGM4 (D1-3 and D4-5) was performed by PCR amplification using proofreading Taq polymerase Phusion Hi as per the manufacturer’s instructions (Invitrogen), full-length codon-optimised TGM4 as template ...
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bioRxiv - Microbiology 2023Quote: ... we initially digested the pNL4-3 plasmid with EcoRI and XhoI and subcloned this fragment into pcDNA3.1/myc-His A (Invitrogen). To generate pcDNA3.1 NL4-3 Env TMTR ...
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bioRxiv - Genetics 2024Quote: ... together with internal size standard ladder where 0.8 µl PCR product was loaded in 9.4 µl Hi-Di Formamide (Applied Biosystems), with 0.1 µl GeneScan 500 LIZ (Applied Biosystems) ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane was incubated overnight at 4°C with anti-His-Tag antibodies (Thermo Fisher Scientific, USA, #MA1-21315) diluted 1:1000 in the blocking solution ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a 6× His-tag was cloned into the BamHI and XbaI sites of the pcDNA 3.1 vector (#V79020, Invitrogen) containing the vascular endothelial growth factor receptor 1 (VEGFR ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were carried out using Recombinant Taq DNA Polymerase (5U/μl from Thermo Scientific) and standard PCR cycling conditions.
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bioRxiv - Cell Biology 2022Quote: ... Purified recombinant plasmid DNA was digested with PacI and transfected into HEK293 cells with Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Recombinant antibodies were transiently transfected and produced in vitro in 293F Freestyle suspension cells (Thermofisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... and recombinant expression was undertaken using the FreeStyle™ MAX 293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2019Quote: ... iPSC line WTC-11 (Coriell Institute) was maintained on recombinant human vitronectin[36,37](Thermo Fisher Scientific), coating six-well tissue culture plastic plates (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... The positive control consisted of 100 – 500 ng recombinant Protein A (ThermoFisher Scientific, Santa Clara, CA). The negative control consisted of ~2 μg wt-TMV from purified N ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant GST-SHP2 in supernatant was affinity-purified by Pierce glutathione agarose (Thermo Fisher Scientific) and eluted with lysis buffer containing 20 mM GSH ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified cell supernatant containing recombinant mAb was passed over Protein A Agarose resin (Thermo Fisher Scientific). Protein A resin was extensively washed with 25 mM Phosphate pH 7.4 ...
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bioRxiv - Zoology 2021Quote: ... The recombinant plasmid was quantified using a NANO-DROP 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). The plasmid copy number was calculated using the equations described by Yun et ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
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bioRxiv - Plant Biology 2020Quote: ... Semi-quantitative PCR was performed within the linear amplification phase using a recombinant Taq Polymerase (Invitrogen) amplifying a fragment of 323 base pairs (bps ...
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bioRxiv - Microbiology 2020Quote: Mice engrafted with J-Lat cells were treated with recombinant human TNF-α (Cat. #PHC3011, Gibco), a potent and well-known LRA ...
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bioRxiv - Cell Biology 2021Quote: ... The amplification reactions for qualitative PCR were performed using Recombinant Taq DNA polymerase (Invitrogen, 11615-038) according to the following parameters ...
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bioRxiv - Immunology 2021Quote: ... Recombinant bacmids encoding each RBDs were generated using the Bac-to-Bac system (Thermo Fisher Scientific) followed by transfection into Sf9 cells using FuGENE HD (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant Cripto protein or transfected with Cripto expression plasmid (pcCripto) using Lipofectamine2000 (Life Technologies, Carlsbad, USA) according to the manufacturer`s instructions ...
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bioRxiv - Cell Biology 2022Quote: Recombinant protein concentration was quantified through BCA protein assay kit (Thermo Fisher Scientific, Buenos Aires, Argentina) on a BioRad 3350 Microplate Reader using BSA as standard ...
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bioRxiv - Pathology 2022Quote: TRP120 and TRX recombinant proteins were desalted using Zeba spin desalting columns (Thermo Fisher Scientific, MA) as indicated by the manufacturer protocol ...
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bioRxiv - Microbiology 2022Quote: ... Genotyping PCR reactions were carried out using Recombinant Taq DNA Polymerase (5U/µl from Thermo Scientific) and standard PCR cycling conditions.
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bioRxiv - Genomics 2022Quote: ... mTESR1 is exchanged with the addition of 5 ng/ml Recombinant Human BMP4 (Fisher Scientific, 314BP010). Exposure to BMP4 triggers differentiation of cells and is considered 0 hours for experimental purposes ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant single-strand AAV2/9 vectors were produced by transfection of HEK293T cells (Thermo Fisher Scientific) with the respective pAAV expression plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 µL of the cleared lysates were added with 20 µL Recombinant Protein G Agarose (Invitrogen) and rotated for 30 min at 4 °C to preclear ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant bacmids encoding each RBDs were generated using the Bac-to-Bac system (Thermo Fisher Scientific) followed by transfection into Sf9 cells using FuGENE HD (Promega ...
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bioRxiv - Microbiology 2021Quote: ... we conjugated recombinant RBD proteins with Alexa Fluor 488 or Alexa Fluor 594 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Quantification of the recombinant proteins was performed by Pierce™ BCA Assay (Thermo Fisher Scientific 23225) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... The recombinant bacmid was extracted and further transfected into Sf9 cells using Cellfectin II (Invitrogen, #10362100). The recombinant viruses were harvested from the transfected supernatant and amplified to generate high-titer virus stock ...
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bioRxiv - Immunology 2022Quote: ... Constant concentration (2 µg 100µl/well) of recombinant SARS-CoV-2 spike protein (RP-87680, Invitrogen) was added to the wells ...
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bioRxiv - Microbiology 2022Quote: ... we conjugated recombinant RBD proteins with Alexa Fluor 488 or Alexa Fluor 647 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... with <0.9% rabbit brain tissue contaminant) and another tube with human recombinant Thromboplastin (Thermo fisher scientific). Next to remove cells ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant BspC was biotinylated using a EZ-Link Sulfo-NHS-Biotin kit (ThermoFisher Scientific, Waltham, MA). The PVDF membrane was probed with the biotinylated BspC (100µg ...
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bioRxiv - Microbiology 2019Quote: ... The Pbc43opt recombinant protein was extracted from inclusion bodies using the Inclusion Body Solubilization Reagent (ThermoFisher). The solubilized protein was also purified using TALON® metal affinity resin ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... cell lysate supernatants were mixed with antibodies and recombinant protein G-Sepharose 4B beads (Thermo Fisher) at 4°C for overnight ...
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bioRxiv - Immunology 2020Quote: ... the reaction mix was supplemented with 40 U RNaseOUT™ Recombinant Ribonuclease Inhibitor (10777019, ThermoFisher Scientific), 200 U SuperScript™ IV Reverse Transcriptase (18090050 ...
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bioRxiv - Plant Biology 2020Quote: ... Serum was used for antibody affinity purification to the STS1 recombinant protein using magnetic beads (Invitrogen). Validation of antibody was carried out by immunoblot with total protein extract from sts1 complemented lines and sts1 mutant as a negative control ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μCi γ32P-ATP and 350 ng CDK1/Cyclin B Recombinant Human Protein (Thermo Fisher PV3292) in kinase buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to red fluorescent Neutravidin beads (Life Technologies). Antibodies were diluted to 5 μg/mL in RPMI-1640 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant RBD was diluted to 2.5 μg/mL in PBS (Thermo Fisher Scientific, Waltham, MA, USA) and 100 μl of the dilution was distributed in the wells of flat-bottom 96-well microplates (Immulon 2HB ...
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bioRxiv - Immunology 2020Quote: ... with oligo(dT) primers in the presence of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) or using SuperScript™ IV VILO™ Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... and analysis of the recombinant bacmid were performed following the manufacturer’s instructions (ExpiSf Expression System, Gibco). ExpiSf9 cells were cultured in ExpiSf9 CD medium to a density of 5×106 cells/mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with recombinant bacmids using Cellfectin II reagent (Gibco-Thermo Fisher Scientific™; Cat#10362100). After a three-day incubation period ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant bacmid was transfected into Sf9 cells using Cellfectin® II reagent (Thermo Fisher Scientific). The Sf9 cells were incubated for 120 hours at 28 to produce P1 baculoviruses ...