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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Human ACE2-transfected HEK293 cells (prepared in-house) were collected and washed with FACS buffer supplemented with 2% FBS (GIBCO, CN: 10099-148) in 1X PBS ...
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bioRxiv - Immunology 2021Quote: ... at the concentration 1×106 cells per ml for TNF-α assessment in myeloid cells or Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) for IFN-γ assessment in T-cells in the presence of protein transport inhibitor (BD GolgiStop™ ...
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bioRxiv - Systems Biology 2020Quote: ... The amount of VEGF contained in the thawed medium was measured using the protocols and reagents from the VEGF Human ELISA Kit (BMS277-2, Thermo Fisher Scientific, USA), and the amounts of PEDF were measured using a Human ELISA Kit (RD191114200R ...
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bioRxiv - Genetics 2020Quote: ... T cells were activated through the T cell receptor subunit CD3 and the co-stimulator CD28 by the addition of Human T-Activator CD3/CD28 Dynabeads at a 1:2 bead:cell ratio (Life Technologies, cat. no. 111.31D) for 72 hours.
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bioRxiv - Microbiology 2020Quote: ... Briefly, parasites were maintained in 2% human O+ erythrocytes (Interstate Blood Bank, Memphis, TN) in RPMI-1640 medium (Life Technologies, Grand Island, NY) supplemented with 0.5% Albumax II (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... [72] at 4 °C overnight followed by staining with 2 µg/ml of secondary AlexaFluor647-conjugated Donkey anti-human IgG antibody (ThermoFisher Scientific, OR, USA) at room temperature for 45 min ...
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bioRxiv - Genomics 2023Quote: ... Cells were rinsed once in staining buffer and incubated with 2 µg/ml anti-human CD326 conjugated with AlexaFluor 700 (ThermoFisher 56-9326-42) at 4C in the dark for 1h ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed once by Hanks’ Balanced Salt Solution (HBSS) and then incubated with 2 μg/mL of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific; A11013) or DyLight 594-conjugated goat anti-human IgG (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: A total of 3×105 CD45Δ T cells (from 2 healthy donors) and T cells (from 2 healthy donors) (effector cells) were mixed with Dynabeads Human T Activator CD3/CD28 (Thermo Fisher Scientific, USA) on the 8th-16th days after knockout of CD45 (E:beads ratio was 1:1 ...
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bioRxiv - Cell Biology 2024Quote: ... 70% confluent EnSC monolayers in 6-well plates were downregulated overnight in 2% DCC-FBS/DMEM/F12 media and transfected with 50nM Stealth siRNA against human GIPC1 or APPL1 (Fisher Scientific, Loughborough, U.K.). For depletion of receptor expression ...
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bioRxiv - Plant Biology 2020Quote: ... 1 µl of the hexane supernatant was then injected in a Trace GC Ultra gas chromatograph coupled to an ISQ mass spectrometer (Thermo Scientific). GC-MS analyses were performed as described in Brückner et al ...
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bioRxiv - Genetics 2020Quote: ... PCR amplification of genomic DNA was performed using Phusion™ Green Hot Start II High Fidelity DNA Polymerase with GC Buffer in presence of 10% of DMSO (ThermoFisher). Big dye sequencing chemistry was used to sequence the PCR products in both directions using the ABI 3500xL Genetic analyser (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... A 1 μL aliquot of the derivative of the supernatant was added to a tube and analyzed using GC-MS (Trace DSQ II, Thermo Scientific). For data processing ...
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bioRxiv - Microbiology 2019Quote: Gas chromatography coupled to isotope-ratio mass spectrometry (GC-IRMS) was performed on a TRACE 1310 Gas Chromatograph (Thermo Fisher Scientific) interfaced with a Scientific GC IsoLink II Conversion Unit connected to an IRMS DELTA V Advantage Isotope-ratio mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2019Quote: GC-FID (Supplementary Figure S1) analysis was accomplished on the TRACE™ 1310 gas chromatograph (Thermo Fisher Scientific™, Austin, TX). The detector temperature was set at 305 °C where the ignition threshold was 0.5 Pa ...
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bioRxiv - Microbiology 2021Quote: ... Sterol profiles for each sample were determined by analyzing the integrated peak areas from GC-MS data files using Xcalibur software (Thermo Scientific). All sterol analysis was performed in biological triplicate ...
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bioRxiv - Microbiology 2021Quote: ... Gas chromatography-mass spectroscopy (GC-MS) (with a Thermo 1300 gas chromatography system coupled to a Thermo ISQ mass spectrometer (Thermo Scientific) was used to analyze and identify TMS-derivatized sterols through comparison of the retention times and fragmentation spectra for known standards ...
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bioRxiv - Microbiology 2021Quote: ... PCR fragments were amplified using appropriate primer pairs (Tables S2 and S3) and Phusion High-Fidelity PCR Master Mix with GC Buffer (Thermo Scientific). Linear fragments were purified on agarose gels ...
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bioRxiv - Microbiology 2020Quote: ... Sterol profiles for each sample were determined by analyzing the integrated peak areas from GC-MS data files using Xcalibur software (Thermo Scientific). All sterol analysis was performed in biological triplicate.
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bioRxiv - Biochemistry 2020Quote: ... The gas chromatography with the mode of Trace-1310 GC coupled to Triple quad mass spectrometer (MS) of the model TSQ8000 from Thermo Scientific is used to perform the analysis ...
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bioRxiv - Biochemistry 2020Quote: ... or mouse monoclonal anti-N-Cadherin clone GC-4 followed by AlexaFluor 647-conjugated goat anti-mouse antibody (1:200 dilution, ThermoFisher Scientific). Staining was performed for 30 min at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... These two constructs were co-transfected in GC-1 mouse testes cells at 80% confluency with Lipofectamine 3000 (Invitrogen Cat# L3000001) using 1.5μL of Lipofectamine and 250 ng of each vector in a well of a 24-well plate ...
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bioRxiv - Systems Biology 2022Quote: ... The preparation of the plasma and urine samples for CCM GC-MS analysis consisted of adding 100% Methanol (LC-MS grade; Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Extracellular associated bacteria identified by reaction with rabbit anti-Gc antibody (Meridian B65111R) that was conjugated in-house with 1 μg/mL Dylight 650 (Thermo Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... 5-10 cortical hemispheres containing axonal GCs were micro-dissected rapidly and homogenized in 0.32M sucrose supplemented with 4mM HEPES (Thermo Fisher, 15630106), Halt protease and phosphatase inhibitor cocktail (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... Mono- and sesquiterpenes were detected using a Trace GC Ultra gas chromatograph coupled with an ATAS Optic 3 injector and an ISQ mass spectrometer (Thermo Scientific) with electron impact ionization ...
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bioRxiv - Plant Biology 2023Quote: ... Mono- and sesquiterpenes were detected using a Trace GC Ultra gas chromatograph coupled with an ATAS Optic 3 injector and an ISQ mass spectrometer (Thermo Scientific) with electron impact ionization ...
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bioRxiv - Microbiology 2023Quote: ... TMS-derivatized sterols were analyzed using gas chromatography–mass spectrometry (GS/MS) (Thermo 1300 GC coupled to a Thermo ISQ mass spectrometer, Thermo Scientific) and identified with reference to relative retention times ...
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bioRxiv - Plant Biology 2024Quote: ... The identification and quantification of cell wall neutral sugars were performed by gas-chromatography (Trace GC Ultra, Thermo Fisher Scientific Inc.) after sulphuric acid degradation with or without a pre-hydrolysis step using 13 M sulphuric acid to determine the amount of glucose residues from acidic-resistant cellulose ...
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bioRxiv - Biochemistry 2024Quote: ... and then in a GC B cell cocktail including: rat anti-mouse CD19 clone 1D3 PerCP-Cy5.5 conjugate (Fisher Scientific, BDB551001), hamster anti-mouse CD95 clone JO2 PE conjugate (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Concentrated virus samples were mixed with Novex™ Tris-Glycine SDS Sample Buffer (2X) (Thermo Fisher Scientific) with NuPAGE™ Sample Reducing Agent (10X ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with virus containing scrambled control or targeting MTR and Polybrene (8 ug/mL, Invitrogen). Cells were split after 48 h into standard RPMI-media (10% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... the virus solution was typically mixed at 1:500 with blue beads (fluorescent polymer microspheres, Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... Reovirus S4 RNA was quantified using the Taq-Man Fast Virus One-Step Master Mix (Applied Biosystems) and GAPDH was detected as an endogenous control using the Pre-Developed TaqMan Assay Reagent kit for mouse GAPDH (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Biochemistry 2021Quote: ... high titer virus generation (V1) was then used to infect 1200 ml High Five™ cells (Invitrogen) for large scale protein production ...
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bioRxiv - Microbiology 2020Quote: ... centrifuged and suspended in virus media that is composed of DMEM high glucose (Thermo Fisher Scientific, USA), with 2% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Master mix for qRT-PCR was prepared using TaqMan Fast Virus 1-step Master Mix (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from 1010 virus particles using the PureLink TM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were generated by Sendai virus reprogramming and clonal expansion using the CytoTune – iPSC 2.0 kit (ThermoFisher) to deliver the reprogramming factors ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: Each RNA sample was subjected to reverse transcription using murine leukemia virus reverse transcriptase (Thermo Fisher Scientific), and the generated cDNA was used as a template for qRT-PCR ...
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bioRxiv - Immunology 2021Quote: ... Reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, USA) and 5 μl of isolated RNA as a template ...
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bioRxiv - Microbiology 2020Quote: ... Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) and a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Microbiology 2021Quote: Virus stock were diluted in 10-fold serial dilutions in 2ml Opti-MEM I (1X) + GlutaMAX (Gibco). 1 ml of each dilution was added to monolayers of 2 × 106 VeroE6 cells in the same medium in a 6-well plate ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNAs were synthesized using Moloney murine leukemia virus RT with random primers (Invitrogen, Thermo Fisher Sci.). RNA levels of representative transcripts were analyzed in triplicate by quantitative PCR with SYBR™ Green using a Bio-Rad CFX thermal cycler ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNAs were synthesized using Moloney murine leukemia virus RT with random primers (Invitrogen, Thermo Fisher Sci.). RNA levels of representative transcripts were analyzed in triplicate by quantitative PCR with SYBR™ Green using a Bio-Rad CFX thermal cycler ...
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bioRxiv - Immunology 2020Quote: ... Virus stocks used in vivo were produced by two passages on Vero E6 cells in DMEM (Gibco) without FBS ...
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bioRxiv - Microbiology 2021Quote: ... Wild-type mumps virus (MuVi/Utrecht.NLD/40.10; genotype G) was grown on Vero cells in DMEM (Gibco) with 2% FBS ...