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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... cells were transduced with Sendai virus (CytoTune – iPS 2.0 Sendai Reprogramming Kit, A16517, Thermo Fisher Scientific) using KOS multiplicity of infection (MOI) ...
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bioRxiv - Microbiology 2023Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Real-time PCR was conducted using TaqMan Fast Virus 1-Step Master Mix from Applied Biosystems in 384-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were infected with virus particles and selected with hygromycin B (200 µg/ml, Invitrogen). For single clone selection ...
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bioRxiv - Microbiology 2024Quote: ... cDNA of the virus genome was generated by reverse transcription (Verso cDNA Synthesis Kit, ThermoFisher Scientific) using MBTuni-12 (5′-ACGCGTGATCAGCAAAAGCAGG ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Physiology 2019Quote: The metabolites were detected using the Trace 1300/ITQ 900 GC-MS system (Thermo Fisher Scientific, USA, Serial No.13005). GC-MS analysis were conducted as previously reported [16] with slight modifications ...
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bioRxiv - Microbiology 2021Quote: ... Eluting peaks were transferred at an auxiliary transfer temperature of 250 °C to a I0-GC mass spectrometer (Thermo Scientific), with a filament delay of 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... Hela cells were plated on cover slips and then transfected with a total of 600 pg of either a scrambled control siRNA oligo medium GC content (Invitrogen) or a mix of three siRNA oligos HSS121071 (Epsin-1) ...
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bioRxiv - Cell Biology 2020Quote: ... For the GC-MS analysis 1 µL of each sample was injected using a PAL autosamplee system (Thermo Fisher Scientifc) using a Split/Splitless (SSL ...
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bioRxiv - Genetics 2021Quote: ... The genomic region surrounding the CRISPR/Cas9 target site (741 bp) was PCR amplified using AccuPrime GC-Rich DNA Polymerase (Invitrogen) (Table S1) ...
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bioRxiv - Biochemistry 2023Quote: ... inside of a GC vial with a 9 mm PTFE/red rubber septum (CAS#C4000-30, Thermo scientific, Rockwood, TN). Samples were placed in an AI/AS 1300 autosampler prior to auto injection ...
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bioRxiv - Molecular Biology 2023Quote: ... All eluting peaks were transferred through an auxiliary transfer line into a Q Exactive-GC-MS (Thermo Scientific, Bremen, Germany). Raw data obtained from data acquired by GC/MS were converted using the open source ProteoWizard’s msConvert software prior to data preprocessing with MS-DIAL 4.6 software (Riken ...
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bioRxiv - Bioengineering 2023Quote: ... The numerical values of total taxanes obtained from the GC-MS TRACE™ 1300 Gas Chromatograph (Thermo Fisher Scientific, UK) were used as a response in the designs ...
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bioRxiv - Systems Biology 2023Quote: ... All eluting peaks were transferred through an auxiliary transfer line into a Q Exactive-GC-MS (Thermo Scientific, Bremen, Germany). The total run time was 25 min ...
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bioRxiv - Immunology 2023Quote: ... 5% (v/v) DMSO if the sequence was GC-rich and UltraPure DNase/RNase-free distilled water (Invitrogen; cat # 10977015) to a final volume of 20 μL ...
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bioRxiv - Microbiology 2023Quote: Analytical gas chromatography of FAME was carried out in a GC-MS Trace 1300 / ISQ 7000 system (Thermo Fisher Scientific) equipped with a BPX70 capillary column (25 m ...
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bioRxiv - Neuroscience 2024Quote: PCR amplification of the DNA has been performed with Phusion high fidelity master mix with GC buffer (ref F532L, ThermoFisher). The primers for PCR amplification have been selected with PerlPrimer.37 The different primers pairs are presented in Table 1.
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bioRxiv - Biochemistry 2024Quote: All siRNAs (siControl: Stealth RNAiTM siRNA Negative Control, Med GC, siMat2a #911: MSS232488) were obtained from Invitrogen (Carlsbad, CA, USA). The target sequence of the siMat2a (911 ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Microbiology 2020Quote: ... To starve Chlamydia of tryptophan HeLa cells were incubated for 24 h in media containing 2 ng/ml recombinant human IFN-γ (Invitrogen: PHC4033) prior to infection ...
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bioRxiv - Cancer Biology 2022Quote: Organoid cultures established from human ACCs were dissociated from Matrigel by incubation with a solution of 2 mg/mL Dispase-II (Thermo Fisher, 17105041) and 200 U/mL Collagenase-III (Worthington ...
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bioRxiv - Cell Biology 2022Quote: Male mouse neuroblastoma Neuro-2a (N2a; ATCC, CCL-131) and female human epithelial HeLa (ATCC, CCL-2) cells were maintained in MEM (Thermo Fisher Scientific), supplemented with 10% FCS ...
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bioRxiv - Microbiology 2021Quote: To facilitate the analyses of invasion assays, HeLa cells (human cervical carcinoma, ATCC® CCL-2™) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Immunology 2022Quote: FLAG-DNAM-1-expressing human Treg cells were co-cultured in complete RPMI-1640 culture medium with 2 × 104 CellTrace Violet (Thermo Fisher Scientific)-labeled Tconv cells (freshly isolated from the same donor as that of the Treg cells ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Genomics 2021Quote: Human brain tissues were manually dissected into small 2-3 mm3 pieces and immersed in homogenization buffer (HBSS (Life Technologies, 14175-095), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... All parasites obtained from other sources or generated in this study were maintained under standard conditions (37) at 2% hematocrit with O+ human erythrocytes in RPMI1640 (Thermo Fisher Scientific) containing hypoxanthine ...
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bioRxiv - Developmental Biology 2022Quote: ... or miR-483-3p 2⍰-O-Methyl antagonist (anti-human; 0, 50 and 100⍰nM in HepG2) using the Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific – 13778100). Cells were harvested and the luciferase assay was performed as previously described21 using the Dual-Luciferase Reporter Assay System (Promega – E1910) ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed two times with FACS buffer to remove any nonbounded Fc-hDectin1 and resuspended in FACS buffer containing a 1:200 dilution of the secondary antibody F(ab’)2-Goat anti-Human IgG conjugated to AlexaFluor™ 488 (ThermoFisher Scientific). This solution was incubated for 1 hour on ice before finally washing twice with FACS buffer and measuring the fluorescence intensity of 5 000 cells using a Guava® flow cytometer with 488nm excitation and fluorescence emission selected using a 522/30 bandpass filter.
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... isolated naïve CD8+ T cells were plated at 2.5 × 105 cells/well in a 24-well plate in 2 ml cRPMI on day 0 and activated with DynaBeads Human T-Activator anti-CD3/CD28 coated microbeads (Thermo Fisher Scientific) and recombinant human IL-2 (rIL-2 ...
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bioRxiv - Genetics 2023Quote: ... cells were stimulated with Dynabeads Human T-Activator αCD3/αCD28 at a bead-to-cell ratio of 1:2 (11131D, Thermo Fisher Scientific). Cell counts were determined every 2-3 days ...
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bioRxiv - Immunology 2023Quote: ... Results of the human samples were developed using a SARS-CoV-2 convalescent plasma as primary and a goat anti-human IgG Alexa Fluor 488 secondary antibody (ThermoFisher Scientific #A48276) (2) ...
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bioRxiv - Physiology 2024Quote: ... at an effector/target ratio of 2:1 in the presence of mouse anti-human CD107a-FITC (11-1079-42; Thermo Fisher Scientific). Cocultures between CTLs and EndoC-βH1 cells expressing HLA-A2 were performed at 37 °C for 1.5 h in Iscove’s Modified Dlbecco’s Medium (IMDM ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The whole tag-attached DRD1 was then cloned into the standard pFastBac (Thermo Fisher) vector ...
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bioRxiv - Developmental Biology 2019Quote: ... which includes both the nuclear H2B-iRFP tag and cell tracker dye (Invitrogen; C12881), Blue and Infra-red fluorescent filter cubes were also used.
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bioRxiv - Bioengineering 2021Quote: ... 50 μg of pre-aliquoted Tandem Mass Tag 6-plex (TMT-6, Thermo Scientific) was resuspended in 20 μL anhydrous acetonitrile ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit monoclonal anti-6x-His tag at 1:1,000 (Thermo Fisher Scientific, MA5-33032), and mouse monoclonal anti-β-actin at 1:10,000 (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with either biotinylated anti-C-tag conjugate (ThermoFisher, Cat. No. 7103252100) for the detection of baits ...
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bioRxiv - Cancer Biology 2020Quote: ... RELA with C-terminal HA tag into Gateway donor vector (pCR8/GW/TOPO, Invitrogen). Then LR clonase II reactions were used to shuttle ORFs into pCW-DEST (lentiviral Dox-inducible expression ...
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bioRxiv - Cell Biology 2021Quote: ... Each sample was labeled with TMT 6 Plex Mass Tag Labeling Kit (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... or 1:7,500 diluted T7-tag polyclonal antibody-HRP (Thermo Fisher, cat# PA1-31449) were incubated with the plate for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... V5-tag-specific antibody was used at 1:100 dilution (Cat # R960-25, Invitrogen) ( ...