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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: EdU assays were performed following the Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher, C10634) manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... Pierce™ Quantitative Colorimetric Peptide Assay (23275) and Pierce™ Rapid Gold BCA Protein Assay Kit (A53227) were ordered from ThermoFisher Scientific (Waltham ...
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Synaptic inhibition in the accessory olfactory bulb regulates pheromone location learning and memorybioRxiv - Neuroscience 2024Quote: ... The BCA assay was performed to quantify the total protein concentration using a Pierce BCA protein assay kit (Thermofisher Cat # 23225). Approximately ...
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bioRxiv - Neuroscience 2024Quote: Primary mouse and human iPSC-derived astrocytes were incubated with Fluo-4 calcium assay reagent (Fluo-4 Direct™ Calcium-Assay-Kit, ThermoFisher) for 30 min at 37°C before recording baseline fluorescence levels (RFUMin ...
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bioRxiv - Biophysics 2024Quote: ... the protein concentration in both the Supernatant (S) and Pellet (P) fractions was determined using the bicinchoninic acid assay (BCA Protein Assay Kit, Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... The protein concentration of the exosome was measured by using a bicinchoninic acid assay (BCA) protein assay kit (Pierce, part of Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The bicinchoninic acid (BCA) assay used for detection of reducing ends was carried out using Pierce BCA Assay Kit (Catalog number 23225, ThermoFisher Scientific). After the starch hydrolysis reactions were cooled to room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... TUNEL assays were performed using the Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection (ThermoFisher cat. # C10617) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was exposed using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: ... which were lysed in Mem-PER plus membrane protein extraction kit (Thermo Fisher scientific) and sonicated 20 min at 100% amplitude and 2/2 pulse ...
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... the immunoblots on the membrane were developed with Chemiluminescent Substrate Reagent Kit (Invitrogen, # WP20005) and were exposed to X-ray film ...
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bioRxiv - Neuroscience 2024Quote: ... stained with Pierce™ Reversible Protein Stain Kit for Nitrocellulose Membranes (Thermo Scientific 24580) and scanned ...
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bioRxiv - Neuroscience 2024Quote: The proteins were transferred to nitrocellulose membrane using iBlot Transfer Stack kit (Invitrogen, NB301001) and iBlot Dry Blot System (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... membrane-RFP 9 were synthesized with the Sp6 mMessage Machine kit (Life Technologies, AM1340) and purified by RNeasy MiniElute Cleanup Kit (QIAGEN ...
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bioRxiv - Genetics 2022Quote: ... Invasion assays were carried out on either inserts coated with 75 μl Matrigel Basement Membrane Matrix (Fisher Scientific Uk Ltd, #11573620) diluted 1:1 with serum-free EVT medium ...
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bioRxiv - Genomics 2020Quote: ... Serum chemistry analysis for ALT and AST levels were performed using commercial assay kits (Infinity Kits, Thermo Scientific) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: Plasma lipids were determined enzymatically using commercially available kits: triglycerides (Infinity Triglyceride colorimetric assay kit (Thermo Fisher, TR22421), non-esterified free fatty acids (WAKO) ...
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bioRxiv - Microbiology 2021Quote: ... Sterol content was measured using the quantitative fluorometric kit ‘Amplex Red Sterol Assay Kit’ (Molecular Probes, Life Technologies). Protein content was measured using the BCA kit (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... Sterol content was measured using the quantitative fluorometric kit ‘Amplex Red Sterol Assay Kit’ (Molecular Probes, Life Technologies). Protein content was measured using the BCA kit (Pierce ...
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bioRxiv - Physiology 2021Quote: ... The concentrations of TG were measured using a triglyceride assay kit (Infinity™ triglycerides kit, Thermo Fisher Scientific) and normalized to tissue weights as previously described13.
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bioRxiv - Cancer Biology 2024Quote: ... Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection kit was used (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... Total protein concentrations were analyzed by using a BCA kit (Micro BCATM Protein Assay kit 23235 Thermo Fisher). The lysates were denatured in 2X SDS Sample buffer at 95°C for 5 mins and run on 15% tris-glycine gel ...
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bioRxiv - Cell Biology 2020Quote: EVs were isolated from FAPs serum free cell culture medium in parallel by ultracentrifugation (UC) and with Total exosomes isolation reagent -TEIR- (#4478359, Invitrogen by Thermo Fisher Scientific) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... pTargeT-αSAT and pTarget-HSATII were performed in parallel in HeLa cells using Lipofectamine® LTX with PLUS™ Reagent (Invitrogen, Thermo Fisher Scientific). Cells were seeded on 22×22mm coverslips or in T-25 flasks such that they were 70-90% confluent at the time of transfection ...
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bioRxiv - Cell Biology 2020Quote: ... pTargeT-αSAT and pTarget-HSATII were performed in parallel in HeLa cells using Lipofectamine® LTX with PLUS™ Reagent (Invitrogen, Thermo Fisher Scientific). Cells were seeded on 22×22mm coverslips or in T-25 flasks such that they were 70-90% confluent at the time of transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 6 min and 10 min parallel reaction monitoring (PRM) methods are described (UltiMate 3000 RSLCnano and PRM–QExactive Plus; Thermo Scientific, Waltham, MA, USA). Raw data were processed using TraceFinder 4.1 (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Final sEVs pellet was resuspended in 100 μL of PBS and the protein content was measured by bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific). The NS500 nanoparticle characterization system (NanoSight ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was collected and the concentration of proteins was measured by BCA(bicinchoninic acid) assay using bovine serum albumin (BSA) as a standard (BCA Protein Assay Kit, Thermo Fisher, 23227). Sample was mixed in loading buffer (P1016 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was removed and the protein concentration was measured by Bradford assay (Pierce Coomassie Plus Assay kit, Thermo Scientific, Catalog #23236). 25µL of DYNAbeads Protein G (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants were transferred to new ice-cold Eppendorf tubes and protein concentration was measured by the BCA assay using Pierce BCA Protein Assay kit (ThermoFisher Scientific; #23225). Equal amounts of protein were loaded on 4-12% SDS-PAGE gel and transferred onto nitrocellulose membrane ...
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bioRxiv - Neuroscience 2021Quote: ... RIPA soluble supernatants were quantified for protein concentration using the BCA assay according to the manufacturer’s protocol (Pierce® BCA assay kit, Thermo Fisher, 23225). RIPA soluble samples (30 μg ...
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bioRxiv - Microbiology 2022Quote: ... the quantity and quality of the samples was tested using standard spectroscopic measurements (Nanodrop One) and using the Qubit 1X dsDNA HS assay and Qubit RNA HS assay kit (Thermo Fisher Scientific). Finally ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The protein content of supernatants was measured using a BiCinchoninic acid Assay (BCA) protein assay kit (Thremo Fisher Scientific, Mississauga, ON, Canada). Equal amounts of protein in lysis buffer (10 µg ...
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bioRxiv - Developmental Biology 2021Quote: ... and translation (HPG) assays were carried out as described previously(Percharde et al. 2018) using Click-iT Assay Kits (Thermo Fisher Scientific) and according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific; cat # 23227). Twenty or 30 µg samples were loaded into SDS-PAGE gels and transferred onto either Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations for the extracellular solution and cell lysate supernatant were determined by BCA assay with Pierce BCA protein assay kit (Thermo Fisher Scientific) according to manufacture protocol on Synergy H1 microplate reader (Biotek) ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were removed and the protein concentration was quantified using the colorimetric assay by PierceTM BCA protein assay kit (ThermoFisher Scientific, 23225). Protein lysates were resolved using 10% SDS-PAGE gels and transferred to 0.45 µm PVDF membranes (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: Fixed brain sections were rehydrated and underwent antigen retrieval before being processed for TUNEL assay using the Click-iT™ Plus TUNEL Alexa Fluor™ 647 Assay Kit for In Situ Detection (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Pure EVs were used for western blot characterization and infection using fractions standardized to the same amount of protein (TPS) determined by the BCA assay (Pierce BCA assay kit, Thermo Fisher Scientific). The graphical scheme of isolation is shown in Figure 1.
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bioRxiv - Developmental Biology 2023Quote: The EdU proliferation assay was performed with the Click-iT™ Plus EdU Alexa FlourTM 488 Flow Cytometry Assay Kit (Invitrogen, C10632), that contains all the necessary reagent except PBS and BSA ...
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bioRxiv - Neuroscience 2024Quote: Cellular cytotoxicity assay has been performed by measuring Lactate dehydrogenase (LDH) in the media by CyQUANT LDH Cytotoxicity Assay kit (Thermo Fisher Scientific), following the manufacturer instructions ...
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bioRxiv - Developmental Biology 2024Quote: Assay was performed on EBs and hPSCs using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Thermo Fisher Scientific). EBs used for the EdU cell proliferation assay were generated and maintained for 5 days using the same protocol as described31,77 ...
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bioRxiv - Biochemistry 2023Quote: ... The amount of lactate in the reaction mix was measured with the Lactate Assay Kit (colorimetric assay) by reading the absorbance using Multiskan SkyHigh plate reader (Thermo Fisher Scientific). Specifically ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed and quantitative β-galactosidase assay was performed using Yeast β-Galactosidase Assay Kit following the manufacturer’s protocols (Thermo Scientific). For Y2H experiments in meiotic conditions ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates were then cleared by centrifugation and protein concentrations determined by Bradford Assay using a Peirce Protein Assay Kit (Thermo Scientific 23200). Equal amounts of protein were denatured in reducing sample buffer ...
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bioRxiv - Systems Biology 2023Quote: ... the concentration of glucose produced from the glycogen was evaluated by using the Amplex Red Glucose/Glucose Oxidase Assay Kit glucose assay (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... OPP (O-propargyl-puromycin) incorporation assays were performed using the Click-iT Plus OPP Protein Synthesis Assay kit (#C10456, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Protein content of the crude mitochondrial fraction was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific catalog number 23225). Thirty µg of mitochondria protein was incubated at 25 °C in 850 µL reaction buffer (100 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... mRNA-LNPs were evaluated for encapsulation efficiency and mRNA concentration using RiboGreen assay using the Quant-iT RiboGreen RNA Assay Kit (Catalog # R11490, Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations of the supernatants were determined via the bicinchoninic acid assay (BCA) using the Pierce BCA Protein Assay Kit (catalog number 23225, Thermo Fisher Scientific). Following BCA-based protein concentration measurements ...