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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was quantified using Pierce BCA Protein Assay Kit (Thermo Scientific). Samples were loaded into a 4-20% Mini-PROTEAN TGX polyacrylamide gel (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined with Pierce BCA protein assay kit (Thermo Fisher) on a 96-well plate format and absorbance measured at 560 nm with Fisher Scientific Multiskan FC plate reader.
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bioRxiv - Evolutionary Biology 2024Quote: ... Library concentration was measured by Qubit dsDNA HS Assay Kit (Invitrogen, #Q33231) and quality was assessed using Bioanalyzer High Sensitivity DNA Analysis Kit (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... Sample concentration was measured using Qubit ds HS Assay Kit (Invitrogen, 2326054) and sample distribution size using Tapestation with high sensitivity D1000 tape (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was measured using BCA protein assay kit (23227, ThermoFisher Scientific). The protein samples were then mixed with Laemmli 4x buffer and boiled for 5 minutes at 95 ...
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bioRxiv - Cell Biology 2024Quote: ... and protein concentration measured by the Pierce BCA protein assay kit (ThermoFisher) following the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... RNA concentration was measured using the QubitTM RNA BR Assay Kit (ThermoFisher) and the integrity of RNA was assessed on a Agilent 2100 Bioanalyzer using the RNA 6000 Nano Kit (Agilent) ...
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bioRxiv - Genetics 2024Quote: ... RNA was quantitated using Qubit RNA BR Assay Kit (Thermo Fisher Scientific). Strand-specific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA concentration was quantified using the Qubit dsDNA HS Assay Kit (Invitrogen) and fragment length was determined using the Fragment Analyzer High Sensitivity NGS Kit (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... Protein in all fractions was estimated using BCA Protein assay Kit (ThermoFisher Cat ...
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bioRxiv - Developmental Biology 2024Quote: ... the concentration was quantified by Qubit dsDNA HS assay kit (Q32854, Invitrogen). 20 ng of cDNA from each sample were used for library construction using the library preparation kit (111KL114 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in membrane blocking solution (ThermoFisher) with 50mM NaF added when phospho-specific antibodies were used ...
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bioRxiv - Cell Biology 2022Quote: ... the retinal cytosol and membrane proteins were separated using the Mem-PER™Plus membrane protein extraction kit (Cat #: 89842, ThermoFisher Scientific, Waltham, MA). The SDS sample loading buffer was added to the cytosol and membrane fractions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then collected in cold PBS and sujected for membrane and cytosol separation using Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, Waltham, MA). Both GC-A and NaK-ATPase ...
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bioRxiv - Microbiology 2020Quote: ... The enzymatic activity of Ddl was also assayed by measuring the release of inorganic phosphate in the reaction (similar to the D-ala-D-ala dipeptide formation assay) with EnzChek phosphate assay kit (Life Technologies). 10 μl of the reaction product was added to 790 μl of standard reaction mixture containing 230 μl dH2O ...
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bioRxiv - Immunology 2020Quote: ... Lysate volumes were subsequently adjusted for equal protein levels following BCA assay (23227, Pierce BCA protein assay kit, Thermo Fisher, USA), and GTP-bound Rac1 levels were assessed according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2021Quote: DNA assay was performed on cells cultured on films and scaffolds using the CyQUANT cell proliferation assay kit (Thermo Fisher Scientific). Lysed scaffolds after ALP assay and frozen films after collection were incubated overnight at 56 °C in Proteinase K solution (1 mg×ml−1 Proteinase K (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cortical lysates were prepared according to the assay type and protein concentration was determined using BCA Protein Assay Kit (ThermoFisher Scientific) prior to the assays ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatants were collected for protein concentration measurement using Bradford assay (Pierce™ Detergent Compatible Bradford Assay Kit, Thermo Fisher Scientific). Protein samples were prepared by mixing protein lysates with 4x SDS protein loading dye at 3:1 ratio ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in Radioimmunoprecipitation assay (RIPA) buffer and protein concentration was determined by using Micro BCA TM protein assay kit (Thermo Fisher). Western blot was performed as described previously (32) ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations of all tissue fractions and TL were determined using the Bicinchonic acid assay (BCA Protein Assay Kit, Thermo Scientific). Bovine serum albumin was used as a protein standard ...
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bioRxiv - Cancer Biology 2021Quote: Cells were lysed in Radioimmunoprecipitation assay (RIPA) buffer and protein concentration was determined by using Micro BCA TM protein assay kit (Thermo Fisher). Protein extracts were loaded onto SDS poly-acrylamide gels and run in 1x Tris-glycine SDS buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The assay was carried out using the Click-iT® HPG Alexa Fluor® Protein Synthesis Assay Kits (ThermoFisher Scientific #C10428). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentrations of the lysates were measured by BCA protein assay using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then unified to 0.5 µg/µL ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 60°C for 15 min and protein concentration was determined by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific). The protein mixture (30∼50 µg ...
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bioRxiv - Microbiology 2020Quote: ... The identity and purity of proteins was confirmed by SDS-PAGE (Coomassie stain) and the quantity determined by Bradford assay (Pierce BCA Protein Assay Kit, Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... Total protein content was determined by the Lowry method (77) adapted to a 96-well plate assay with the Microplate BCA Protein Assay Kit (23252, Thermo Scientific), using bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... 45µl of the working buffer (D-luciferin and the Firefly Glow Assay buffer according to manufacturer’s instructions, Pierce Firefly Luciferase Glow Assay Kit, Thermo Fisher Scientific) was added to the cell lysate and we waited 10 minutes before acquiring signals using a microplate reader (Tecan ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and the protein assay was performed using the Pierce BCA Protein Assay kit (Cat#23225, ThermoFisher Scientific). The supernatants from different samples were adjusted to the same protein concentration of protein and the same volume of the supernatants were subjected to acetylcholine assay using QuickDetectTM Acetylcholine (ACh ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were normalised to 2 µg/µl in radioimmunoprecipitation assay (RIPA) buffer using the Qubit® Protein Assay Kit (ThermoFisher Scientific) prior to processing as described in McVeigh et al ...
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bioRxiv - Neuroscience 2021Quote: The concentrations of protein samples from human brain whole homogenate preparations were measured via the bicinchoninic acid (BCA) assay (Micro BCA protein assay kit, ThermoFisher Scientific). 20 μg of total protein were mixed 1:1 with Laemmli buffer and boiled at 95 °C for 10 minutes ...
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bioRxiv - Immunology 2020Quote: ... A single library pool was compiled using equimolar concentrations of DNA as measured using a fluorometric assay (Qubit dsDNA Broad-Range Assay kit, Invitrogen, UK). The Illumina MiSeq platform (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... Measurement of final protein concentration was performed using BCA protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™, #23227), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentration was determined using a bicinchoninic acid (BCA) assay from the Pierce™ BCA Protein Assay Kit (Thermo Scientific). The fluorescence spectra of the EYFP samples were measured with a Varioskan LUX Multimode Microplate Reader (Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2021Quote: Triglyceride content of samples was measured using a GPO-PAP Triglyceride assay (Randox Laboratories Ltd) and cholesterol using Amplex Red Cholesterol Assay Kit (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Total protein amounts of the OM cell lysates were quantified with BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared by adding standard radioimmunoprecipitation assay (RIPA) buffer and normalized using a BCA protein assay kit (Thermo Scientific). Proteins were separated by sodiumdodecyl sulphate-polyacrylamide gel electrophoresis (SDS–PAGE ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were supplemented with SDS to a final concentration of 2% and the protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Neuroscience 2022Quote: ... and protein concentration for each sample was determined using a BCA assay (Pierce Microplate BCA Protein Assay Kit, ThermoFisher, Waltham, MA). Equal amounts of protein were prepared using 4x NuPage sample buffer (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... The purified proteins and protein standards were subjected to a colorimetric bicinchoninic acid (BCA) assay following the manufacturer’s protocol and then stored at −80°C until use (Pierce protein assay kit; ThermoFisher Scientific, USA). The proteins were visualized by running a 4-20% SDS-PAGE (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: The OPP assay was performed using the Click-iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The protein concentration of the supernatant was determined by bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Fisher Scientific). 100 μg of protein were loaded into FASP filters (Amicon Ultra 30K ...
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bioRxiv - Biochemistry 2023Quote: The inhibitory effect of a panel of four small-molecule inhibitors was determined using a modification of the amylase activity assay in CVLs used above (EnzCheck Ultra Amylase Assay Kit ThermoFisher, E33651). For initial screening ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentration was measured by the bicinchoninic acid (BCA) assay using the Pierce BCA protein assay kit (ThermoFisher Scientific: 23225). Equal amounts of protein were loaded on 4 to 12% SDS-PAGE gel and transferred onto nitrocellulose membrane ...
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bioRxiv - Cell Biology 2023Quote: ... The collected supernatant was used to measure protein concentration using the BCA assay (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was used to determine the protein concentration by BCA assay according to the manufacturer’s instructions (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; ThermoFisher Scientific; cat # 23227). Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences ...
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bioRxiv - Molecular Biology 2023Quote: ROS assay was performed using a Total Reactive Oxygen Species Assay Kit 520nM (Cat. Nr. 88-5930-74, Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration in supernatant was determined using bicinchoninic acid (BCA) protein assay (PierceTM BCA Protein Assay Kit, Catalog #23225, ThermoFisher Scientific). 30-40 µg total protein sample was denatured at 75 °C for 7.5 min and then electrophoresed using polyacrylamide gel (4–15% tris-glycine extended polyacrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: EdU assays were performed following the Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher, C10634) manufacturer’s guidelines ...