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Citations for Thermo Fisher :
2101 - 2150 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Transient transfection with plasmids was done either using Lipofectamine 2000 (Invitrogen) or Xfect (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... followed by addition of plasmid DNA in conjunction with lipofectamine2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 500ng of plasmid and 2uL of lipofectamine 2000 (Invitrogen, Cat #11668019) were each diluted in 50uL Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... or the PureLink HiPure Plasmid Midiprep kit (catalog number K210005; Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... plasmid (1.5 µg) was overexpressed using Lipofectamine 2000 (Invitrogen, 11668-019). After 24 hours post transfection ...
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bioRxiv - Cell Biology 2023Quote: ... transfection of plasmid vectors was carried out using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid was digested with BsmBI (FastDigest Esp3I, Thermo Fisher Scientific) and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was purified using the GeneJet Plasmid Miniprep Kit (Thermo Scientific) and verified by full-length plasmid sequencing (Plasmidsaurus) ...
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bioRxiv - Biochemistry 2023Quote: ... Concentration of all plasmids was measured by NanoDrop One (Thermo Scientific) and the plasmids were stored at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... 4 μg plasmids were mixed with 8 μL Lipofectamine 2000 (Invitrogen) and the mixture was added to cells followed by incubation at 37 °C for at least 24 h ...
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bioRxiv - Immunology 2023Quote: ... All plasmids were transfected using Lipofectamine 2000 (11668019, Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were transiently transfected using Lipofectamine 3000 Transfection Reagent (Thermo Scientific) according to the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... The sen cDNA was ligated into plasmid blunt II Topo (Invitrogen) and then transformed into TOP10 competent Escherichia coli cells ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid concentration was measured by a NanoDrop Spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... and 30.4 μg of plasmid DNA diluted in Opti-MEM (Gibco). Media was replaced with fresh culture medium after 7 h and cells were harvested 48 h post-transfection.
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with the corresponding plasmid using Lipofectamine 2000 (Invitrogen) following the manufacture recommendations and selected with 300 ug/mL of geneticin for 10-14 days ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid transient transfection was performed using Lipofectamine 3000 (Invitrogen, Carlsbad, CA) and siRNA transfection was conducted using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... All transient plasmid DNA transfections were done using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... adenoviral plasmids were transfected into 293A cells using Lipofectamine 2000 (Invitrogen). 293A cells were cultured in DMEM (Sigma 5796) ...
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bioRxiv - Cell Biology 2022Quote: ... The miRNA plasmid was transfected using Lipofectamine LTX (Thermo Fisher Scientific) as described in manufacture’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Purified ligated plasmids were electroporated into MegaX DH10B (Thermo Fisher Scientific) and plated on LB/Amp plates ...
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bioRxiv - Developmental Biology 2023Quote: ... The entire construct was cloned into a pJet1.2 plasmid backbone (ThermoFisher) using Gibson assembly master mix (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid constructs were transfected using Lipofectamine® 2000 Reagent (Invitrogen) as per the manufacturer’s instructions72h after transfection 1.2mg/ml of G-418 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Positive colonies containing pYES2/NT C plasmids (Thermo Fisher, Cat#: V825220), expressing N-terminal His-tagged CSLD2 ...
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bioRxiv - Developmental Biology 2023Quote: ... at a ratio of 1:1.5 plasmids: lipofectamine 3000 (Invitrogen, L3000001) overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... and prepared using the PureLinkTM Quick Plasmid Miniprep Kit (Invitrogen, #K210010). Plasmid identification was verified via Sanger sequencing.
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bioRxiv - Immunology 2023Quote: ... and CAR plasmids were transfected into HEK293T host cells (in Gibco DMEM HG medium containing 10% FBS ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... Transfection of plasmid DNA was performed using Lipofectamine 3000 (Invitrogen L3000015) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmid pJK14-Tn4rev was designed using Vector NTI software (Life Technologies) and synthesized de novo by GENEWIZ Gene Synthesis Services (GENEWIZ ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmid transfections were carried out using Lipofectamine 2000 (Thermo Fisher) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... and transferred into Ti plasmids by multisite Gateway LR reaction (Thermofisher) for simultaneous expression of right and left platinum TALENs fused to the Arabidopsis delta prime subunit mitochondrial presequence ...
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bioRxiv - Synthetic Biology 2024Quote: ... pAAV cTnT HyPerDAAO plasmid was cleaved with SacI (Thermo Scientific, FD1133) and BglII restriction enzymes (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 250-500 ng plasmid and 1 μL Lipofectamine 3000 reagent (Invitrogen) were mixed in Neurobasal medium for a well and incubated for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... were transfected with 2.5 µg pLightswitch reporter plasmid and pMIR (Ambion). 24 hours post-transfection ...
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bioRxiv - Physiology 2023Quote: ... plasmids were transiently transfected into cells using Lipofectamine 3000 (Invitrogen #L3000015) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... codon-optimized spike expression plasmid using Lipofectamine 2000 (Invitrogen™ 11668019) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: All the plasmids and siRNAs were transfected using Lipofectamine 2000 (Invitrogen) and RNAiMAX (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... faecalis and the PureLink® Quick Plasmid Miniprep Kit (Invitrogen, USA) to isolate plasmid DNA from E ...
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bioRxiv - Molecular Biology 2023Quote: ... contaminating genomic or plasmid DNA was removed using ezDNase (Invitrogen 11766050), followed by reverse transcription using SuperScript IV VILO master mix (Invitrogen 11766050) ...
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bioRxiv - Microbiology 2023Quote: ... the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific GmbH, Schwerte, Germany) was used to purify the plasmid DNA after cloning the PCR products using the CloneJET™ PCR Cloning Kit with DH10B Competent Cells (Thermo Fisher Scientific GmbH ...
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bioRxiv - Microbiology 2023Quote: ... pET_AV101_TSP1-4 extracted with GeneJET Plasmid Miniprep Kit (Thermo Scientific™). Vectors carrying the correct tsp insert (pET_AV101_TSP1-4 ...
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bioRxiv - Microbiology 2023Quote: ... This was purified using the GeneJET plasmid miniprep kit (Thermo Fisher), and linearised using NdeI (NEB ...
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bioRxiv - Microbiology 2023Quote: ... plasmid DNA and Expifectamine were mixed in Opti-PRO SFM (Gibco) according to the manufacturer’s instructions and added to the cells ...
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bioRxiv - Bioengineering 2023Quote: ... ExpiFectamine reagent and plasmid DNA were diluted into OPTIMEM (Gibco™) according to the amount determined by the manufacturer’s transfection protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids were transfected with Lipofectamine™ 3000 Transfection Reagent (Invitrogen; # L3000015), according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transfected into cell culture using lipofectamine 2000 (Invitrogen, 11668019) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified PCR products were then cloned into the pDONR221 plasmid (Invitrogen). For CpomIR41a1 ...
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bioRxiv - Neuroscience 2023Quote: ... DNA plasmid electroporation was performed with NEON Transfection System (Invitrogen, MPK5000) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: Primary neurons were transfected with expression plasmids using Lipofectamine 2000 (Invitrogen). In brief ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfection was performed using Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) according to the manufacturer’s instruction ...