Labshake search
Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... The resulting sgRNA plasmid was linearized by FastDigest DraI (Thermo Scientific) and served as template for sgRNA in vitro transcription using the T7 MEGAshortscript Kit (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... Plasmid DNA and siRNA oligonucleotides were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2019Quote: ... Plasmid constructs were introduced via Neon electroporation (Invitrogen, Carlsbad, CA, USA) as previously described (16) ...
-
bioRxiv - Molecular Biology 2019Quote: ... the respective plasmids were digested with FastDigest PvuI (Thermo Fisher Scientific).
-
bioRxiv - Synthetic Biology 2021Quote: ... with vacuum manifold and GeneJET Plasmid Midiprep Kit (Thermo Fisher Scientific) were used for higher yields ...
-
bioRxiv - Biochemistry 2019Quote: ... 0.5 μg plasmid DNA in 200 μl Opti-MEM (Life Technologies). After incubation for 15 min ...
-
bioRxiv - Plant Biology 2020Quote: ... which were then purified using the Purelink Plasmid Midiprep Kit (Invitrogen) and introduced into rice protoplasts as described previously (Kawano et al ...
-
bioRxiv - Cell Biology 2019Quote: ... Transfections with plasmid DNA were performed using Lipofectamine2000 reagent (Thermo Fisher) according to manufacturer’s recommendation ...
-
jouvence, a new human H/ACA snoRNA involves in the control of cell proliferation and differentiationbioRxiv - Genetics 2020Quote: ... p3000 and the plasmids were previously diluted in OptiMEM 1X (Gibco) (a Reduced-Serum Medium) ...
-
bioRxiv - Genetics 2021Quote: ... or the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, MA) according to the provided protocols ...
-
bioRxiv - Genomics 2021Quote: ... were cloned and purified with HiPure Plasmid Midiprep Kit (Invitrogen; K210005).
-
bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were isolated using Gene-JET Miniprep Kit (Thermo Scientific, K0503). Isolated plasmids were verified with Sanger sequencing.
-
bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfections were carried out using Lipofectamine 2000 Transfection Reagent (Invitrogen) following the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2021Quote: ... cloned into Zero Blunt® TOPO® plasmid (Thermo Fisher Scientific), and sequenced.
-
bioRxiv - Plant Biology 2021Quote: ... cloned into Zero Blunt® TOPO® plasmid (Thermo Fisher Scientific), and sequenced ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmids of interest were transfected using Lipofectamine 3000 (Thermo Fisher Scientific) or jetPRIME® (Polyplus transfection ...
-
bioRxiv - Cell Biology 2020Quote: ... Transfection of plasmid DNA was performed using Lipofectamine 3000 (Life Technologies) according to the manufacturer instructions.
-
bioRxiv - Biochemistry 2021Quote: ... DNA was purified using the PureLink plasmid midiprep kit (Invitrogen, CA), filtered through a 0.22 μm filter ...
-
bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was extracted using a GeneJET miniprep kit (ThermoFisher, K0503) and sequence-confirmed with Sanger sequencing on an Applied Biosystems 3730 Genetic Analyzer.
-
bioRxiv - Molecular Biology 2021Quote: ... Plasmid was transfected into cells using the Lipofactamine 3000 reagent (ThermoFisher) following the manufacture’s protocol ...
-
bioRxiv - Genomics 2020Quote: ... 5ug of plasmid library and 175μl of ssDNA (UltraPure, Thermo Scientific) were added to the cells ...
-
bioRxiv - Microbiology 2021Quote: ... using the PureLink HiPure Plasmid MidiPrep kit (Invitrogen, Thermo Fisher Scientific). Sequences near the AgeI and KasI cloning sites were confirmed by Sanger sequencing in both directions as described above ...
-
bioRxiv - Microbiology 2021Quote: ... using the PureLink HiPure Plasmid MidiPrep kit (Invitrogen, Thermo Fisher Scientific). Sequences near the AgeI and KasI cloning sites were confirmed by Sanger sequencing in both directions as described above ...
-
bioRxiv - Microbiology 2020Quote: ... 4 µg plasmid and 6 µl Turbofect (Thermo Fisher Scientific, USA) were combined ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmid s were purified using the GeneJET Miniprep kit (Thermo Scientific). DNA fragments were purified using the Purelink PCR purification kit (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... plasmid DNA and Expifectamine were mixed in Opti-PRO SFM (Gibco) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... The plasmid vectors (0.265 pmol) were transfected using Lipofectamine 3000 (Invitrogen) into HEK293T cells cultured in Dulbecco’s Modified Eagle’s Medium (Gibco ...
-
bioRxiv - Molecular Biology 2020Quote: ... plasmids were extracted using the miniprep kit (Fisher Scientific, cat # FERK0503) and sent to DNA sequencing to verify the plasmid sequence.
-
bioRxiv - Immunology 2021Quote: ... coli and purified using a GeneJET plasmid miniprep kit (Thermo Fisher). To express anti-DEC205-OVA ...
-
bioRxiv - Genomics 2021Quote: ... Transfect the constructed plasmid into the cells(Invitrogen, Cat. No. L3000075), and observe the expression under a fluorescent microscope 24 hours later.
-
bioRxiv - Genomics 2021Quote: ... cells were transfected with individual reporter plasmid using Lipofectamin 2000 (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The pDonor221 and pLenti6/V5- DEST plasmids were purchased from Invitrogen. The pENTR1A-GFP-N2 (#19364 ...
-
bioRxiv - Neuroscience 2020Quote: ... pRSVr and pMDLg-pRRE helper plasmids using Lipofectamine2000 (Thermo Fisher Scientific) into HEK293T cells ...
-
bioRxiv - Cell Biology 2022Quote: ... Plasmid transfections into 293FT cells were performed with LipofectaminTM 2000 (Invitrogen). One day later ...
-
bioRxiv - Biophysics 2022Quote: ... 200 ng of plasmid DNA and 0.4 μL P3000 reagent (Invitrogen) were diluted in 15 μL pre warmed opti-MEM and vortexed briefly ...
-
bioRxiv - Developmental Biology 2022Quote: ... plasmids were introduced into ElectroMAX Stbl4 Competent Cells (Life technologies #11635018) using MicroPulser Electroporator (Bio-Rad #1652100 ...
-
bioRxiv - Cell Biology 2022Quote: ... SUN2 cDNAs were cloned into the pcDNA5-FRT-TO plasmid (Invitrogen). For CTDNEP1-rescue experiments a lentiviral vector was used with the expression of CTDNEP1 being driven by the EF-1α promoter ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Plasmid extraction and DNA gel purification kits were purchased from ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... The fragment was cloned into the pUC19 plasmid (Thermo Fisher Scientific) using the Hindlll/BamHI restriction sites ...
-
bioRxiv - Molecular Biology 2022Quote: ... purified and cloned in the plasmids pCR8/GW/TOPO® (Invitrogen) using the primers listed in Supplementary Table S2 ...
-
bioRxiv - Biochemistry 2022Quote: ... U2OS cells were transfected with plasmid DNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions and analysed 24 hours after transfection ...
-
bioRxiv - Microbiology 2022Quote: ... PCR and cloned into the TOPO-TA plasmid (Thermo Fisher Scientific). The human wild-type GBA1-containing TOPO-TA plasmid was then used as a template to generate the N370S and L444P GBA1 variants ...
-
bioRxiv - Neuroscience 2022Quote: ... or pCINeo (transfection control plasmid) using Lipofectamine 2000 reagent (Thermo Scientific). Cells were fixed using 4% paraformaldehyde (PFA ...
-
bioRxiv - Molecular Biology 2023Quote: ... the plasmid was digested with BsmBI (FastDigest Esp3I, Thermo Fisher Scientific) and a synthetic 391 bp double-stranded DNA fragment encoding 5′-(1st gRNA/scaffold/H1 promoter/2nd gRNA)-3′ was inserted using the NEBuilder HiFi assembly system (NEB) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasmids were cut using appropriate restriction enzymes (Fast Digest-Thermo Scientific), dephosphorylated with FastAP and purified from an agarose gel with a Zymoclean Gel DNA Recovery kit (Zymo Research) ...
-
bioRxiv - Microbiology 2023Quote: ... pyciF was purified (GeneJET plasmid miniprep kit, Thermo Fisher Scientific, Lithuania) and confirmed by restriction digestion to visualize the insert release and Sanger sequencing (Aggrigenome ...
-
bioRxiv - Molecular Biology 2022Quote: ... The expression kit containing pPICZαB plasmid was purchased from Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with pCBA-SceI plasmid using Lipofectamine 2000 (Invitrogen). Cells were harvested 48 hrs post-transfection ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 500 ng TORNADO Broccoli expression plasmid using Lipofectamine 3000 (Invitrogen). After 48 and 72 hours ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmid transfection was performed using Lipofectamine 3000 Reagent (Thermo Fisher Scientific), in accordance with the manufacturer’s instructions.