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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Recombinant Mouse Erbb2 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Volumes of lysate containing equal amounts of total protein were used to immunoprecipitate RACK1 by incubating with mouse monoclonal antibody against HA and protein G agarose beads (Thermo Fisher, 15920010) at 4°C with gentle mixing ...
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bioRxiv - Cell Biology 2019Quote: ... and subjected to immunoprecipitations with goat anti-GFP antibodies (generic goat IgGs as control) or mouse anti-γ-adaptin antibodies (mouse hybridoma C7 antibodies as control) bound to protein G-Sepharose beads (Fisher Scientific). Samples were rotated end-over-end at 4°C for 2 h and washed 2 x in buffer D+ and 1 x in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Immunology 2022Quote: ... the cells were first extracellularly stained with a mouse anti-human monoclonal antibody APC-Cy7 conjugated LIVE/DEAD™ Fixable Near-IR Dead Cell Stain (#L10119, Invitrogen, Thermo Fischer Scientific, OR, USA), following a similar preparation procedure as just described for the DCs ...
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Precision genetic cellular models identify therapies protective against endoplasmic reticulum stressbioRxiv - Neuroscience 2020Quote: ... cells were incubated for 30 min at RT with Alexa-488 labeled secondary goat anti-mouse antibody (ThermoFisher Scientific, cat. A32723, 1:1000), washed again twice with PBS and analyzed using flow cytometry on a FACSCelesta cytometer (BD Biosystems) ...
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bioRxiv - Genomics 2019Quote: ... we washed the slide and incubated with fluorescently-labeled secondary antibodies (1:500, Alexa Fluor 488 anti-rabbit & Alexa Fluor 594 anti-mouse (Invitrogen, Carlsbad, CA, USA) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed with FACS buffer and then incubated with Alexa Flour 488-labeled goat anti-rabbit or goat anti-mouse IgG (Invitrogen, A11034; Invitrogen, A11001) for 1 h at 4 °C ...
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: Myristoylated GFP (myr::GFP) was labeled with 1:500 mouse anti-GFP (Sigma-Aldrich, Cat#G6539, RRID:AB_259941, or ThermoFisher, Cat#A-11120, RRID:AB_221568)
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bioRxiv - Microbiology 2023Quote: ... was used as primary antibody (dilution 1:300) and Alexa Fluor 568 labeled donkey anti-mouse IgG (H+L) (Invitrogen, cat no. A10037) as secondary antibody (dilution 1:1000).
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bioRxiv - Cancer Biology 2023Quote: ... sections were incubated for 1 h with appropriate secondary antibodies to either rabbit or mouse IgGs labeled with Alexa Fluor 488 (1:200 dilution, ThermoFisher, Catalog No. A21206) and Cy3 (1:200 dilution ...
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bioRxiv - Cancer Biology 2021Quote: Single live cell suspensions were washed with 1% FCS in PBS and resuspended in flow cytometry buffer (2% FCS and 1 mM ethylenediaminetetraacetic acid (EDTA; Thermo Fisher #AM9260G) in PBS).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Random primer (fc 50 ng/µl) and RNase Inhibitor (fc 1 unit/µl) in 1X First Strand synthesis buffer (Invitrogen/Life Technologies). Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... Flag-tagged BNIP3 was cloned into a pcDNA3.1 vector (Thermo Fisher Scientific) or a pCDH vector (System Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Tagged constructs were transfected using Lipofectamine 2000 reagent (Life Technologies, CA, USA) following the manufacturer’s instructions using a 1:3 ratio of DNA:Lipofectamine ...
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bioRxiv - Neuroscience 2020Quote: ... and biotin-tagged DNA was pulled down with streptavidin beads (Invitrogen, 65001) and ligated with Illumina paired-end adapters ...
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bioRxiv - Cell Biology 2022Quote: ... dextran polymers tagged with Alexa Fluor™ 680 (3,000, 10,000 MW, Thermofisher) and dextran tagged with fluorescein (40,000 MW ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged strains were taken from the 4000-GFP yeast library (Thermofisher), while knockout strains were taken from the Yeast Knockout Collection (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... These were then tagged using 4 different multiplex TMT isobaric tags (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... corresponding species-specific Alexa-Fluor (488 or 555)-tagged antibodies (Molecular Probes) were incubated for 1h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Specific antibody labeling was detected using fluorescence tagged secondary antibodies from Invitrogen. Details of primary antibodies used are as follows ...
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bioRxiv - Immunology 2021Quote: ... was incubated with purified GST-tagged IKKi (catalog # PV4875; Thermo Fisher Scientific) in buffer A containing 20 mM Tris [pH 7.5] ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-tagged donkey-anti-rabbit (Molecular Probes, 1:1000) secondary antibodies for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-tagged strains were taken from the 4000-GFP yeast library (Thermofisher), RFP-tagged strains were taken from the localization database collection (Huh et al ...
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bioRxiv - Immunology 2023Quote: ... tagged ribosomes were eluted with 1mg/ml HA peptide (Thermo Scientific 26184) for 15 minutes at 30°C ...
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bioRxiv - Pathology 2024Quote: ... Wash with PBS and stain fluorescence-tagged rabbit secondary antibodies (Invitrogen, USA) for visualization ...
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bioRxiv - Immunology 2020Quote: ... with 10% heat-inactivated foetal calf serum (FCS) (Gibco,), 2 mM glutamine ...
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bioRxiv - Cell Biology 2020Quote: ... complemented with 10% fetal calf serum (FCS; Life Technologies) in a humidified incubator (Eppendorf ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% FCS and 1% penicillin/streptomycin (Invitrogen). HEK293 cells stably transfected with TLR4 cDNA (HEK/TLR4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... in a microplate reader (Multiscan FC, ThermoFisher Scientific, USA). The Fe(II ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco), and used for the experiment between passage 6 and 8.
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bioRxiv - Microbiology 2020Quote: ... and 10% heat-inactivated fetal calf serum (FCS) (Gibco).
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bioRxiv - Immunology 2019Quote: ... supplemented with 10% dialyzed FCS (Thermo Fisher Scientific, # A3382001), 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... plus 10% heat-inactivated fetal calf serum (FCS, Gibco). Transfection assays were performed using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: Isolated single cells were blocked with Fc block (Invitrogen) for 5 mins and stained for myeloid cell surface markers using the following antibody panel ...
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bioRxiv - Cancer Biology 2019Quote: ... New medium with 10% CS-FCS (12676-011, Gibco) with or without 100 nM T3 was added and cells were incubated for a further 2 days ...
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bioRxiv - Biochemistry 2020Quote: ... MR-Fc was quantified by Nanodrop (Thermo Fisher Scientific) and stored at −20°C until further use.
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bioRxiv - Neuroscience 2021Quote: ... The proteinase K was inactivated with 10 % FCS (Gibco) in R16 medium ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% foetal calf serum (FCS) (Life Technologies) and 1% penicillin/streptomycin (P/S) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FCS (Thermo Fisher, UK, Cat: 11560636). Cell lines were confirmed mycoplasma free at 6-monthly intervals ...
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bioRxiv - Biophysics 2020Quote: ... and inserted into a human Fc expression vector (Invitrogen). The proteins were expressed in HEK293T cells ...
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bioRxiv - Cell Biology 2021Quote: ... containing 1% FCS and 1% GlutaMAX (35050– 061, Gibco) under standard tissue culture conditions (5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... using a microplate reader (Thermo Fisher Scientific, Multiskan FC). The antibody titer was defined as previously described [33].
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bioRxiv - Microbiology 2022Quote: ... containing 10% heat-inactivated fetal calf serum (FCS, Gibco) at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1X penicillin-streptomycin and 10 % FCS (Thermo Fisher Scientific). Where indicated ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS) (Gibco, ThermoFisher) and 2 mM L-glutamine (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS) (Gibco, ThermoFisher) and 2 mM L-glutamine (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Life Technologies) in a humidified atmosphere at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% of Fetal Bovine Serum (FCS, Gibco), 2 mM glutamine and 25 µg/ml gentamicin (Gibco).