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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Recombinant Mouse Erbb2 protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody: donkey anti-mouse Alexa 594 antibody, ThermoFisher), and with fluorescent Nissl and DAPI ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then incubated with Alexa Fluor donkey anti-mouse IgG 488-labeled secondary antibody (1:1000 dilution, Thermo Fisher Scientific) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... After incubation with fluorescently labeled secondary antibodies (α-rabbit 488, α-mouse 488, α-rabbit 647, and α-rabbit 568; Thermo Fisher), coverslips were mounted with DAPI ProLong Gold (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated for 45 minutes at room temperature with 1:500 dilutions of the appropriate labeled secondary antibody (Alexa Fluor 546 goat anti-guinea pig, Thermo Fisher, A11074; Alexa Fluor 546 goat anti-mouse, Thermo Fisher, A11003 ...
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bioRxiv - Microbiology 2020Quote: ... followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml; Thermo Fisher Scientific, Poland). Actin filaments was stained using phalloidin coupled with Alexa Fluor 633 (0.2 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with fluorescein isothiocyanate-labeled goat anti-rabbit and anti-mouse secondary antibody (Alexa Fluor; Invitrogen; Thermo Fisher Scientific, Inc.). DAPI was applied to each section for 10 min to counterstain the nuclei.
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with fluorescein isothiocyanate-labeled goat anti-rabbit and anti-mouse secondary antibody (Alexa Fluor; Invitrogen; Thermo Fisher Scientific, Inc.). DAPI was applied to each section for 10 min to counterstain the nuclei.
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bioRxiv - Biochemistry 2022Quote: ... and Alexa Fluor 488- or 568-labeled donkey anti-rabbit or goat anti-mouse secondary antibodies (1:1,500 dilution, Life Technologies, A10042, A11029), and mounted with DAPI-containing VECTASHIELD mounting medium (Vector Labs) ...
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bioRxiv - Bioengineering 2020Quote: ... we fluorescently labeled samples by incubation with 1:500 dilution of secondary Alexa Fluor 594 goat anti-mouse (ThermoFisher Scientific, A-11005) and ATTO 647N goat anti-rabbit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Bound primary antibody was then labeled for 1h at room temperature using Alexa488 goat anti-mouse IgG secondary antibody (Invitrogen, OR, USA) at a 1:750 dilution in PBS ...
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bioRxiv - Microbiology 2022Quote: ... ME) using the EasySep™ mouse T cell isolation kit (StemCell, Cambridge, MA) and labeled with carboxyfluoroscein succinimidyl ester (CFSE; Life Technologies) as described previously [18] ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed two times and resuspended in FACS buffer containing the Alexa Fluor-488-labeled secondary antibodies at a 1:200 dilution: goat anti-mouse (Invitrogen cat. A11001), goat anti-rabbit (Invitrogen cat ...
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bioRxiv - Bioengineering 2019Quote: ... 5×106 cells were labeled with a 1:100 dilution of chicken anti-c-myc antibody or mouse anti-V5 antibody (Thermo Fisher Scientific) for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed twice with cold PBA and labeled for 20 min on ice with Alexa 488-conjugated anti-mouse IgG1 antibodies (1:400, ThermoFisher reference A21121). For the Refrex1/DC16HA ligand ...
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bioRxiv - Immunology 2023Quote: ... After counting in hemocytometer cells were labeled with mouse monoclonal antibodies using the markers live/dead Aqua (Life Technologies, Carlsbad, CA, USA), -CD45 ...
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bioRxiv - Cell Biology 2023Quote: ... Mono-biotinylated Fab’ fragments of goat anti-mouse Ig(G+M) were labeled with AF546 using an Alexa Fluor 546 antibody labeling kit (Thermo Fisher Scientific). The molar ratio of AF546 to Fab’ in the AF546-labeled Fab’ was ∼2 determined by a Nanodrop spectrophotometer (Nanodrop Technologies).
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were purified from the spleens of 1C6 mice using mouse CD4 MicroBeads (Miltenyi) and labeled with CellTrace Violet (CTV; Thermo Fisher Scientific). CTV-labeled 1C6 CD4+ T cells were cultured with B cells at a ratio of 1 CD4 ...
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bioRxiv - Developmental Biology 2022Quote: ... The following antibodies and dyes were used for extracellular staining with a FACS buffer containing 10% FBS in PBS: CD31-APC with mouse specificity (1:500, Thermo Fisher Scientific, 17-0311-82) together with its IgG2a kappa APC-Isotype control (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... Then recombinant dsDNA fragment (add to > 400 ng/μl final concentration) or recombinant single-strand DNA (ordered from Invitrogen or IDT ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc and DC-SIGN-Fc and then stained with Alexa Fluor® 488 goat anti-human IgG antibody (Life Technologies) diluted 1/200 in PBS + 1% FCS or BB buffer and incubated 30 min on ice ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Random primer (fc 50 ng/µl) and RNase Inhibitor (fc 1 unit/µl) in 1X First Strand synthesis buffer (Invitrogen/Life Technologies). Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays ...
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bioRxiv - Biophysics 2021Quote: ... The actin cytoskeleton was labeled with Alexa Fluor 488- or 568-labeled phalloidin (1:400; Thermo Fisher). All the imaging experiments presented in this work were conducted using a Nikon A1R confocal scope equipped with 20x (air ...
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bioRxiv - Cancer Biology 2023Quote: ... single dye labeled cells and tri-labeled cells were pelleted and fixed with IC fixation buffer (Invitrogen) every other day from day 0 to day 12.
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bioRxiv - Microbiology 2020Quote: ... the recombinant proteins were produced in their respective expression systems according to standard procedures and purified using either Ni-NTA (ThermoFisher Scientific, Waltham, MA, USA) or Strep-Tactin (IBA Lifesciences ...
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bioRxiv - Immunology 2021Quote: ... and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen, catalog no. AM2694), and the resulting RNA/DNA was tested for viral copies by qRT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 μg/ml of recombinant Tat using a commercial transfection reagent Pro-ject Protein Transfection Reagent Kit (Thermo Scientific Cat. No. 89850) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-green fluorescent protein (GFP) at 1:000 (RRID: AB_221568, Invitrogen, Carlsbad, CA).
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-green fluorescent protein (GFP) at 1:000 (RRID: AB_221568, Invitrogen, Carlsbad, CA). Rabbit anti-leucokinin (Nässel et al. ...
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bioRxiv - Microbiology 2021Quote: ... For samples that were mixed with protein (BSA, human CAR, mouse CAR [Fisher Scientific]) prior to attachment ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were analyzed on an immunoblot probed with mouse anti-6XHis antibody (Invitrogen) and horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... with the Mouse Monocyte Chemoattractant Protein-1/CCL2 (MCP-1) Uncoated ELISA Kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Total protein was isolated from cells or mouse tissue using RIPA buffer (Thermo Fisher), or lysates were enriched for histone proteins using Histone Extraction Kit (Abcam) ...
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bioRxiv - Immunology 2023Quote: ... Macrophages were incubated with the labelled proteins or anti-mouse F4/80 (ThermoFisher Scientific) for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... TDP-43 protein clusters were visualised using anti-mouse AlexaFluor488 antibody (1:2000, ThermoFisher), incubated for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and supplemented 1:2000 with Neuron Growth Factor (Ngf 2.5S Native Mouse Protein, Invitrogen) in the right ...
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bioRxiv - Biophysics 2020Quote: All pET28a-N proteins (WT and mutants) and nsp12 protein were labeled by incubating with a 1:1 molar ratio of Alexa Fluor™ 546 carbox (Thermo Fisher Scientific) for 1 h at room temperature with continuous stirring ...
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bioRxiv - Neuroscience 2021Quote: ... Biotin molecules were labeled at the N-terminal primary amino group (-NH2) of the bait proteins using N-hydroxysuccinimide (NHS)-biotin (ThermoFisher Scientific, Waltham, MA) in PBS at pH 6.5 ...
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bioRxiv - Neuroscience 2023Quote: The surface proteins of HEK293 and hippocampal cells were biotin-labeled and isolated using a Pierce cell surface protein biotinylation and isolation kit (A44390, Thermo Scientific, IL, USA). The protein samples were resolved using 10% SDS PAGE and transferred to polyvinylidene fluoride membranes ...
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bioRxiv - Cell Biology 2023Quote: ... Volumes of lysate containing equal amounts of total protein were used to immunoprecipitate RACK1 by incubating with mouse monoclonal antibody against HA and protein G agarose beads (Thermo Fisher, 15920010) at 4°C with gentle mixing ...
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bioRxiv - Cell Biology 2019Quote: ... and subjected to immunoprecipitations with goat anti-GFP antibodies (generic goat IgGs as control) or mouse anti-γ-adaptin antibodies (mouse hybridoma C7 antibodies as control) bound to protein G-Sepharose beads (Fisher Scientific). Samples were rotated end-over-end at 4°C for 2 h and washed 2 x in buffer D+ and 1 x in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... the cells were first extracellularly stained with a mouse anti-human monoclonal antibody APC-Cy7 conjugated LIVE/DEAD™ Fixable Near-IR Dead Cell Stain (#L10119, Invitrogen, Thermo Fischer Scientific, OR, USA), following a similar preparation procedure as just described for the DCs ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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Precision genetic cellular models identify therapies protective against endoplasmic reticulum stressbioRxiv - Neuroscience 2020Quote: ... cells were incubated for 30 min at RT with Alexa-488 labeled secondary goat anti-mouse antibody (ThermoFisher Scientific, cat. A32723, 1:1000), washed again twice with PBS and analyzed using flow cytometry on a FACSCelesta cytometer (BD Biosystems) ...
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bioRxiv - Genomics 2019Quote: ... we washed the slide and incubated with fluorescently-labeled secondary antibodies (1:500, Alexa Fluor 488 anti-rabbit & Alexa Fluor 594 anti-mouse (Invitrogen, Carlsbad, CA, USA) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed with FACS buffer and then incubated with Alexa Flour 488-labeled goat anti-rabbit or goat anti-mouse IgG (Invitrogen, A11034; Invitrogen, A11001) for 1 h at 4 °C ...