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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Pig Histone H3.3 H3F3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Culture supernatants at different time intervals post infection/ treatment were filtered through a 0.2μ filter and subjected to ELISA by using the eBioscience (Thermo Fisher Scientific Inc. USA) ELISA kit as per recommended protocols.
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bioRxiv - Cell Biology 2020Quote: The concentration of IFNλ1/IL-29 was measured in basolateral medium from RSV BT2a- or mock-infected cultures at 96 hpi by ELISA (Thermo Fisher Scientific; BMS2049). The manufacturer’s instructions were followed.
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bioRxiv - Biochemistry 2021Quote: ... The reaction was developed using TMB (3,3⍰,5,5⍰-Tetramethylbenzidine) substrate (1-Step™ Ultra TMB-ELISA Substrate Solution; Thermo Scientific, Waltham, MA, USA). Absorbance was measured at 450 nm with Multiskan™ FC Filterbased Microplate Photometer (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Interleukin-8 (IL-8 or CXCL8) protein in the basolateral media was measured using a magnetic bead-based ELISA (Procartaplex, ThermoFisher Scientific, Waltham, MA) according to the instructions provided by the manufacturer and the plate were then read using a Luminex-based Bio-plex Multiplex system (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:3000 (PBS milk 1% -Tween 0.1%) for 1 h at 25°C and detected using 1-Step(tm) Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Waltham, MA). Plates were read using Cytation5 (BioTek ...
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bioRxiv - Immunology 2021Quote: ... plates were washed six times with PBST and developed by adding 50 μL of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific 34022) per well ...
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bioRxiv - Immunology 2020Quote: ... Native ECP was coated at 10 μg/ml (IgA) or 20μg/ml (IgG) on ELISA plates (Nunc Maxisorp™, ThermoFisher Scientific, Waltham, MA). Bile was diluted to 1:200 and serum was diluted to 1:20 in PBS containing 2.5 % ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed 3 times with WB before adding 100 µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher 34029) followed by incubation in the dark at room temperature with shaking (225 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... SAP97 or SAP102 fused to a C-terminal MBP were coated overnight at 4°C on ELISA plates (Nunc Maxisorb Immunoplates, #442404, Thermo) at a concentration of 100 ng per well ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then incubated for 24 hours to induce macrophage activation and cytokine release.48 ELISA (Invitrogen, Waltham, MA, Product #88-7066-22) was performed to quantify the level of IL-6 present in the cell media ...
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bioRxiv - Biochemistry 2023Quote: ... three times washed with 1%BSA (prepared in 1XTBS) followed by development of signal by treating cells with 200μL TMB-ELISA (Thermo Scientific, Cat no. 34028) until the light blue color appeared ...
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bioRxiv - Bioengineering 2022Quote: ... Plates were finally washed three more times with PBS-T and developed using 1-Step Ultra TMB-ELISA solution (Thermo Fisher Scientific #34028). The HRP reaction was stopped with 1M hydrochloric acid and absorbance values at 450 nm were measured on a SpectraMax i3x plate reader (Molecular Devices).
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bioRxiv - Immunology 2023Quote: ... E40500) to stop the reaction and the absorbance was measured at 450/620 nm using an ELISA plate reader (Thermo Fisher, Varioskan LUX). The mean OD (optical density ...
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bioRxiv - Immunology 2020Quote: Cytokine concentrations for IL-17A and IFNγ from the supernatants of cultured cells and ex vivo colon organ cultures were measured by ELISA (Thermo Fisher Ready-Set-Go). Samples and standards were measured in duplicate ...
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bioRxiv - Biochemistry 2021Quote: ... plates were washed 4 times with TBS-T and proteins were detected by addition of SuperSignal™ ELISA Femto substrate (ThermoFisher cat. no. PI37074). Luminescence was read using a BMG POLARstar Omega plate reader or Biotek Neo2 microplate reader.
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bioRxiv - Microbiology 2020Quote: Chimeric proteins were diluted to 1 μg/ml in carbonate buffer (pH 9.6) and used to coat polystyrene 96-well high-binding ELISA plates (100 μl/well; Nunc-Immuno plate with MaxiSorp surface). After overnight incubation at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Mouse cytokine levels in vaccinated animals (IL-4, IL-10, IL-12, and IFN-γ) was measured by ELISA following the supplier’s protocol (Invitrogen or R&D Systems).
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bioRxiv - Microbiology 2020Quote: Rabbit serum immunized with DENV-1 Mochizuki strain (in-house product) was coated onto a Nunc Maxisorp ELISA plate (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Pathology 2022Quote: ... The reaction was stopped with 3N H2SO4 (50 μL/well) and read at 492 nm on a MultiSkan GO ELISA plate reader (Thermo Fisher Scientific, Vantaa, Finland) [35].
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bioRxiv - Bioengineering 2019Quote: ... conditioned media collected from cells was assessed with enzyme-linked immunosorbent assays (ELISA) in order to quantify the concentration of extracellular tumor necrosis factor alpha (TNFα, Invitrogen, cat. #88-7346-88) or Dex (Bioo Scientific ...
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bioRxiv - Immunology 2020Quote: ... were diluted in 50 mM carbonate buffer to a concentration of 1 μg/ml and coated at 50 ng/well on 96-well ELISA plates (MaxiSorp Nunc, Thermo Fisher Scientific K.K., Japan) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... incubated at RT for 10 min and the absorbance was recorded at 540 nm using a 96-well plate ELISA reader (Thermo Fisher, Rockford, IL, USA).57 The concentration of nitrite was calculated according to the standard curve generated from known concentrations of sodium nitrite dissolved in fresh culture media.
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bioRxiv - Microbiology 2021Quote: ... Specifically, 1 μg/ml purified hACE2 protein (Kactus, ACE-HM501) was coated onto a 96-well ELISA plate (Thermo Fisher Scientific, 44-2404-21) at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... The bacteria were cultured at 37°C for 48 hours and the concentrations were measured at multiple time points by the ELISA plate reader (Multiskan Go, Thermo Scientific, Waltham, MA, USA) at the OD of 600nm.
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bioRxiv - Immunology 2023Quote: Peptide competitions were undertaken by incubating RAVV GP ΔMuc at 200 ng per a well in Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Direct pepscan analysis was undertaken by binding 200 ng of each overlapping peptide to Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were collected following a 48h incubation at 37°C and IFNγ and TNFα levels were measured by ELISA (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Neuroscience 2020Quote: ... Blots were developed using Amersham ECL Prime Western Blotting Detection Reagent (RPN2232) or SuperSignal ELISA Femto Maximum Sensitivity Substrate (Thermo Scientific, Ref: 37075, Lot: UO282555) and the resulting chemiluminescence imaged with an Alpha Innotech MultiImage Light Cabinet (Alpha Innotech ...
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bioRxiv - Immunology 2020Quote: ... were diluted in 50 mM carbonate buffer to a concentration of 1 μg/ml and coated at 50 ng/well on 96-well ELISA plates (MaxiSorp Nunc, Thermo Fisher Scientific K.K., Japan) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound target-Fab complexes were incubated with an HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Formalin-killed N61 in PBS was dried in an oven at 5×106 cells/well in 96-well ELISA plates (C96 Maxisorpcert, Nunc-Immuno Plate, Thermo Fisher Scientific) overnight at 37°C ...
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bioRxiv - Plant Biology 2024Quote: Coating: Prepared sample were added to the selected ELISA-suitable antigen-binding 96-well microtiter plate (CORNING COSTAR 9018; Thermofisher scientific Ref: 44-2504-21). The first two columns contained the peptide standard ...
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bioRxiv - Biophysics 2021Quote: Plates were incubated with the secondary antibody dilutions for one hour at 37 °C and developed with 1-Step™ Ultra TBM-ELISA (Thermo Scientific™, Waltham, MA, USA) reagent according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: 50 µl of AP205 VLP solution (5 µg/ml) was pipetted into an appropriate number of wells of ELISA plates (4 Nunc MaxiSorp flat bottom 96-well plates) and the VLP allowed to adsorb overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 96-well ELISA plates were coated with coating buffer (Na2CO3 0.1 M pH 9.6) containing IL-12p70 coating antibody (Invitrogen, clone 20C2 M122 and used at 3 μg/ml) overnight and at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: DNA assay kit QuantiT dsDNA HS kit (Invitrogen) was used to quantify the DNA content from cell lysate according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2023Quote: ... following kit instructions (BCA Protein Assay kit, ThermoFisher #23225) and 20µg loaded into each well of a NuPAGE 4-12% Bis-Tris Gel (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... mitochondria isolation kit and IL-6 kit were from Invitrogen and were purchased from ALT Ukraine Ltd (representative of Thermo Fisher Scientific in Ukraine).
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bioRxiv - Molecular Biology 2023Quote: ... kit and a TURBO DNA-free Kit (Thermo Fisher Scientific), the RNA was then further purified using RNeasy kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Kit (ThermoFisher Scientific) and subsequently 10 clones per cell line were sequenced (LightRun GATC Carlo Erba) ...
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bioRxiv - Genetics 2020Quote: ... coli kit (Invitrogen). The transfection was performed according to the manufacturer’s guidelines and vector assembly was tested by Sanger sequencing.
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bioRxiv - Systems Biology 2022Quote: A kit (Ambion Magmax™-96 total RNA isolation kit ...
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bioRxiv - Physiology 2023Quote: Transcription Kit (Invitrogen). The qPCR was performed in a QuantStudio 6 Flex Real-Time PCR System (Life Technologies) ...
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bioRxiv - Biophysics 2023Quote: Kit(Ambion AM1340M). They were injected at the one-cell stage together with the microspheres:
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bioRxiv - Microbiology 2024Quote: ... kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR cloning kits from Thermo Scientific (TOPO TA Cloning Kit 450030) and New England Biolabs ...
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bioRxiv - Synthetic Biology 2020Quote: ... GeneJET gel extraction kit and plasmid miniprep kit (Thermo Fisher Scientific) were used for DNA extraction ...
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bioRxiv - Genetics 2021Quote: A commercially available kit (Click-iT EdU Cell Proliferation Kit, ThermoFisher) was used to label proliferating cells with thymidine analog 5-ethynyl-2’- deoxyuridine (EdU) ...