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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... After 4-5 h bacteria were collected by centrifugation (5000 rpm, SLA 3000 rotor, ThermoFisher Cat#07149) for 15 minutes and resuspended by gentle trituration in ice-cold 50 ml of TBS Buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... heated for 5 minutes at 95C and separated on 4-12% NuPAGE® Bis-Tris gel (Invitrogen) by electrophoresis and subsequently transferred onto Immobilon-P™ PVDF membranes of 0.45 μm pore size (Millipore® ...
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bioRxiv - Microbiology 2024Quote: ... containing 100 mg/ml of X-gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Thermofisher) to confirm CFU/ml counts.
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bioRxiv - Microbiology 2024Quote: ... harvested cells by centrifugation (10,015 x g, 5 min, 4 °C, Heraeus Biofuge Primo R, Thermo Scientific), washed the cell pellets twice in TE buffer (10 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... Pancreatic tissues were incubated for 24 hours at 4°C in ≥5 volumes of RNAlater solution (ThermoFisher) to preserve RNA integrity ...
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bioRxiv - Developmental Biology 2023Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 5 min prior to loading onto NuPAGE 4-12% Bis-Tris gels (Invitrogen). After washing with water and then with 5% glycerol ...
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bioRxiv - Cancer Biology 2023Quote: ... then 5 × 107 cells were lysed in 4 ml Pierce IP Lysis Buffer (87788; Thermo Fisher Scientific) for 20 minutes on ice ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were boiled for 5 minutes before loading duplicate 4-12% Bis-Tris SDS-PAGE gels (ThermoFisher) and running gel electrophoresis (1 hour ...
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bioRxiv - Microbiology 2019Quote: ... after which time 1 μL FM 4-64 (Life Technologies, 1 μg/μL in DMSO) was added to the suspension and gently mixed ...
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bioRxiv - Neuroscience 2022Quote: ... probenecid (1:250) and PowerLoad concentrate (1:100) (Fluo-4 Calcium Imaging Kit, Molecular Probes) in BrainPhys basal media for 30 min (37 °C ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were passaged 1:2-1:6 every 2-5 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 μl/cm2 ...
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bioRxiv - Genomics 2020Quote: ... 10 mL B-27 Minus Insulin (A1895601, ThermoFisher Scientific), 5 mL Glutamax (35050-061 ...
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bioRxiv - Cancer Biology 2019Quote: ... antibiotics and 10 μg/ml hygromycin B (Thermo Fisher). All cell lines were obtained from American Type Culture Collection (ATCC ...
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bioRxiv - Developmental Biology 2023Quote: ... with 10% FBS and B-27 Supplement (ThermoFisher, 17504044). The same media were used during flow cytometry sorting ...
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bioRxiv - Microbiology 2024Quote: ... Tenfold dilutions were used to infect confluent Vero E6 cells in a 96-well plate in MEM (5% FBS, 1% penicillin-streptomycin, 1% kanamycin, 3% amphotericin B (Gibco)) at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: Bone marrow B factions were sorted and calcium influx was assayed with a Fluo-4 Direct™ Calcium Assay Kit (ThermoFisher cat# F10471). Briefly cells were incubated with Fluo-4 Direct™ at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... we lysed cell pellets by adding the following components to each gram of pellet: 4 mL of B-PER (Thermo Fisher Scientific, Inc.), 1 mg MgSO4 ...
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bioRxiv - Microbiology 2020Quote: ... The assay is based on the dilution of co-mixed N-(7-nitro-benz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine (N-NBD-PE) and N-(lissamine Rhodamine B sulfonyl)phosphatidylethanolamine (N-Rh-PE) (Molecular Probes, Eugene, OR, USA), whereby dilution due to membrane mixing results in increased N-NBD-PE fluorescence ...
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bioRxiv - Neuroscience 2020Quote: ... Neurovascular components were then pooled and re-suspended in 4 mL of buffer B (HBSS 1X Ca2+/ Mg2+ free with phenol red (Gibco, Waltham, MA, USA), 10 mM HEPES (Gibco ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... Neurovascular components were then pooled and re-suspended in 4 mL of buffer B (HBSS 1X Ca2+/ Mg2+ free with phenol red (Gibco, Waltham, MA, USA), 10 mM HEPES (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were incubated with 10 µM 5-ethynyl-2’ deoxyuridine (EdU, Thermo Fisher) for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... All medium were supplemented with 2-5 or 10% fetal bovine serum (FBS; Gibco) according to the experimental setting ...
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bioRxiv - Physiology 2024Quote: ... 25 μM Cy5-hydrazide with 10 mM 2-amino-5-methoxybenzoic acid (ThermoFisher Scientific) in 1xPBS as a catalyst was added to the cells for 15 minutes at room temperature to allow complete conjugation with the aldehyde ...
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bioRxiv - Cancer Biology 2021Quote: ... submerged in 10 mL of freshly prepared 4% PFA in 1X PBS (Thermo Fisher Scientific, #28906), and placed on a rocker set at low speed for 24 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μL of sample were resolved on NuPAGE 4-12 % Bis-Tris gels (Thermo Fisher Scientific) using MOPS running buffer ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Denatured protein (10-20 µg) was loaded per lane on 4-12% Bis-Tris Gels (Invitrogen). Protein was transferred onto PVDF membrane ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 µg of denatured proteins were loaded into a 4-12% gradient polyacrylamide gel (ThermoFisher Scientific) and transferred onto Invitrolon PVDF membranes (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... Formulation P-0 or S-50 was fixed for 10 minutes with 4 % formaldehyde (Fisher Scientific), embedded in frozen section compound (VWR) ...
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bioRxiv - Cell Biology 2021Quote: ... Electrophoresis was performed on NuPAGE 4-12% or 10% Bis-Tris Gel (Invitrogen, NP0302BOX and NP0315BOX) in 1x MOPS SDS running buffer (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed twice in HBSS for 10 minutes and loaded with Fluo-4 AM (Invitrogen) for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were resolved on pre-cast 4-12 % NuPAGE 1.5 mm 10 well SDS gels (Invitrogen). The gels were rinsed with deionised water and placed in an X-cell Surelock Mini-cell running tank ...
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bioRxiv - Microbiology 2021Quote: ... heated to 95°C for 10 min separated on NuPAGE 4-12% Bis-Tris Gels (Invitrogen) for 90 min at 120 V and blotted at constant 30 V for 30 min onto Immobilon-FL PVDF membrane (Merck Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... 10% glycerol) were separated on 3-8% Tris-acetate or 4-12 Bis-Tris gels (Thermofisher). Proteins were then transferred to PVDF membranes (Merck).
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bioRxiv - Microbiology 2021Quote: All western blot analysis was performed using 4-12% Bis-Tris 10-well protein gels (Invitrogen) and NuPage MES SDS running buffer (Novex) ...
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bioRxiv - Microbiology 2021Quote: ... and proteins were separated on 4-12% or 10% Bis-Tris SDS-PAGE gels (Thermo Fisher). To assess disulphide cross-linking in cells ...
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bioRxiv - Neuroscience 2021Quote: DRG neurons were incubated in 10 μM of the Ca2+ indicator Fluo-4 AM (Invitrogen, UK) for 30 min at room temperature (21°C) ...
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bioRxiv - Cancer Biology 2020Quote: ... Thawed samples were run on pre-cast 4-12% or 10% Bis-Tris gels (Life Technologies) using NuPage MOPS Buffer (Life Technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... 4 ng GST or GST-VDAL purified protein were loaded onto 10 kDa microspin columns (Ambion). After washed twice with 1 mL HS buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Pathology 2021Quote: Cells (4×105) were seeded onto 60mm plates in 10% FBS DMEM (ThermoFisher Scientific, Gibco #11995123). The next day the cells were washed with PBS and transferred to serum-free DMEM (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2021Quote: Cells (4×105) were seeded onto 60mm plates in 10% FBS DMEM (ThermoFisher Scientific, Gibco #11995123). The next day the cells were washed with PBS and transferred to serum-free DMEM (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Samples were boiled for 10 minutes in 4% β-mercaptoethanol and 1x sample buffer (Thermo Scientific) and loaded on a 10% Bis/Tris gel with MES or MOPS running buffer for SDS-PAGE analysis ...
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bioRxiv - Immunology 2020Quote: ... During centrifugation at 16500 × g for 10 min at 4 °C 500 μl streptavidin beads (Invitrogen) or each sample was equilibrated in a 1:1 mixture of lysis buffer and 50 mM Tris-HCL pH 7,4 ...
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bioRxiv - Neuroscience 2022Quote: ... and loaded into a NuPAGE 4%–12% or 10% Bis-Tris Gel (Invitrogen, Cat# NP0336BOX, NP0301BOX) alongside a protein ladder (Thermo Fisher Scientific Cat#26619 ...
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bioRxiv - Bioengineering 2022Quote: ... and 10 μL of solution was loaded into a 4-20% Tris-glycine gel (ThermoFisher #XP04200PK2) along with 3 μL of Precision Plus protein ladder (Bio-Rad #1610374) ...
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bioRxiv - Immunology 2022Quote: ... 10 μl of sample were loaded in a 4-12% Bis-Tris pre-cast gel (Invitrogen). Gel was transferred to a PVDF membrane and blocked in 1% BSA (Fisher ...
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bioRxiv - Genetics 2022Quote: ... 10 μg protein lysates were loaded into a NuPAGE 4%-12% 17-well gel (NP0329BOX, Invitrogen), and transferred onto nitrocellulose membranes ...
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bioRxiv - Physiology 2024Quote: ... sections were washed 4 × 10 min in PBS and mounted with ProLong™ Gold antifade (Invitrogen). Muscle sections were imaged at the Biozentrum Imaging Core Facility with an Axio Scan.Z1 Slide Scanner (Zeiss ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µg of total DNA was digested with 4 µl of FastDigest HincII (Thermo Scientific™) for ∼16 hours at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 μg of protein was resolved on 4-12% Novex WedgeWell SDS-PAGE gels (Invitrogen). Following electrophoresis ...