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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and 2% B-27 supplement (Life Technologies cat# 17504044) in an incubator with 5% CO2 at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... We labeled the purified RLC with 10 molar excess of 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS, Invitrogen) or dabcyl C2 maleimide (AnaSpec ...
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bioRxiv - Bioengineering 2024Quote: ... and splenocyte to resuspend post centrifugation for 5 minutes and subsequently diluted with 5mL ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS ...
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bioRxiv - Biophysics 2023Quote: ... 300 mL NaCl, 5 mM imidazole, 10% glycerol, 0.2 mM 2-mercaptoethanol, 1 mM PMSF, and Pierce Protease Inhibitor Tablets [Thermo Fisher]). Cells were sonicated for 5 min (30% amplitude ...
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bioRxiv - Developmental Biology 2023Quote: ... with 10% FBS (Gibco One-Shot FBS, UK) (charcoal stripped to deplete hormones) ...
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bioRxiv - Cancer Biology 2020Quote: ... The cDNA synthesis reaction was diluted 1:50 and 4 μL was used for a 10 μL qPCR reaction with PowerUp™ SYBR™ Green Master Mix (ThermoFisher) and primers specific for the reporter mRNA (Table S5 ...
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bioRxiv - Physiology 2020Quote: ... containing 1.5 ml of chilled (4°C) transfer media composed of Ham’s F-10 medium including 1 mM L-glutamine (LG; Thermo Fisher Scientific, Denmark), 0.1% heat inactivated fetal bovine serum (hiFBS ...
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bioRxiv - Microbiology 2021Quote: One-tenth of the protein samples and their corresponding inputs (pre-diluted 1/10) were size-separated on a bolt 4-12% Bis-Tris plus (Thermo Fisher Scientific) and stained with the silver stain kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Red blood cell cultures (~4% hematocrit) were crosslinked for 10 min by direct addition of methanol-free formaldehyde (1% final concentration) (Thermo Fisher # 28908). The cross-linking reaction was quenched using a final concentration of 0.125 M Glycine for an additional 5 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... J774A.1 macrophages were seeded at 4×105 macrophages/well in RPMI with 10% FBS in sterile 16-chamber slides (Nunc Int., Denmark). After incubation ...
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bioRxiv - Neuroscience 2022Quote: Fixed slices containing biocytin-filled neurons were washed with PBS 4 x for 10 minutes and transferred to a solution containing AlexaFluor Streptavidin 647 (Invitrogen, 1:500) and Neurotrace 435/55 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: Approximately 5 × 106 MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h) were fixed with 1% methanol-free formaldehyde solution (Thermo Scientific, #28908) for 10 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... the coverslips were washed and fixed with 4% paraformaldehyde in PBS for 10 min at room temperature then stained with Alexa Fluor 488 Phalloidin (1:500; Invitrogen cat# A12379) in blocking buffer for 20 min at room temperature and mounted in DuoLink PLA mounting medium with DAPI ...
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bioRxiv - Immunology 2022Quote: 5 × 106 MC/9 cells were suspended in 0.5 ml of optiMEM (GIBCO) and kept at room temperature for 10 min ...
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bioRxiv - Immunology 2022Quote: ... 5×106 MC/9 cells were suspended in 0.5 ml of optiMEM (GIBCO) and kept at room temperature for 10min before electroporation was performed as stated above.
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bioRxiv - Neuroscience 2023Quote: ... Cultures were maintained in NGM (500 ml Neurobasal-A Medium, 10 ml B-27, and 5 ml Glutamax, from Gibco, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were maintained in NGM (500 ml Neurobasal-A Medium, 10 ml B-27, and 5 ml Glutamax, from Gibco, ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... and 100 μg/ml cycloheximide using Beckman Coulter UC tubes 9/16 x 3-1/2 (Fisher Scientific, NC9194790), and equilibrating overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were subsequently incubated for 2 hours at 4°C with Streptavidin Dynabeads (Invitrogen M-280), and beads were successively washed with RIPA (2 times) ...
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bioRxiv - Immunology 2019Quote: ... Tissue was washed for 24h before nuclear staining with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher). Images were acquired using a Zeiss LSM 710 confocal (Carl Zeiss) ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in PBS with 30nM of 4’,6-diamidino-2-phenylindole (DAPI, Life Technologies). In each tube ...
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bioRxiv - Neuroscience 2019Quote: ... slices were stained by 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI: Thermo Fisher Scientific, Yokohama, Japan) across several experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... and then the nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) for 5 min ...
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Single-cell protein analysis reveals metastable states during the transition to a sensory organ fatebioRxiv - Developmental Biology 2021Quote: ... Then they were stained with 0.5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), and mounted in Vectashield (Vector Labs) ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleus staining was performed using 4’,6-diamidino-2-phenylindole (DAPI) (3 mM, D3571, Molecular Probes). Cells were counted from four randomly selected fields per culture under a confocal microscope (TCS SP8 ...
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bioRxiv - Microbiology 2021Quote: ... and stained with 200 μl of 1.0 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS ...
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bioRxiv - Physiology 2020Quote: ... brain tissue was removed and rapidly frozen using 2-methylbutane (Thermo Fisher Scientific, Cat# O3551-4) at -50°C to prevent tissue cracking ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were separated in a SDS-PAGE (2-4% gradient gel, NuPage™; ThermoFisher Scientific) and blotted to a PVDF membrane (300 mA ...
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bioRxiv - Cell Biology 2021Quote: ... Nuclei were counterstained using 5μg/ml of DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride; D1306, Invitrogen) added during secondary antibody incubation.
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bioRxiv - Cancer Biology 2021Quote: ... : DAPI Buffer (106mM MgCl2, 50 µg/mL 4’, 6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat# D1306), 5mM Ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Physiology 2022Quote: ... CellTracker Green CMFDA Dye (C2925) and DAPI (4’,6-diamidino-2-phenylindole) (D1306) were from Invitrogen. EZ-Link Sulfo-NHS-LC-LC-Biotin (#21135 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Nuclei were then stained with 0.01% w/v 4’,6-diamidino-2-phenylindole dilactate (DAPI, Invitrogen) for 5 min and rinsed with ddH2O ...
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bioRxiv - Neuroscience 2022Quote: ... Non-viable cells were excluded by staining with 4’,6-diamidino-2-phenylindole (Thermo Fisher, D1306). Flow-cytometric data were analyzed using FlowJo software (BD Biosciences).
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were counterstained with 300nM 4’,6-diamidino-2-phenylindole dilactate (DAPI) (D1306, ThermoFisher Scientific, UK) for imaging assessment (Zeiss Axio observer Z1 microscope) ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were stained with 300 nM 4’,6-diamidino-2-phenylindole nucleic acid stain (DAPI; Invitrogen) in PBS for 5 min followed by washing three times in PBS at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and then flash-frozen in dry-ice-chilled 2-methylbutane (−40 °C) (#03551-4, Fisher Scientific). Regions of interest were collected from these slices using a 1 mm biopsy micropunch (#15110-10 ...
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bioRxiv - Immunology 2021Quote: ... however Live/Dead stain was not performed and instead 4’-6’-diamidino-2-phenylindole (DAPI, ThermoFisher) was added immediately prior to sorting for live/dead detection ...
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bioRxiv - Neuroscience 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) and Leica standard immersion oil were obtained from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at 4°C and incubated with 40ul washed Dynabeads Protein G (Invitrogen 10003D) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclei were identified based on 4′,6-diamidino-2-phenylindole (DAPI; purchased from Thermo Fisher Scientific) staining before measurement of nuclear γH2AX and 53BP1 staining ...
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bioRxiv - Microbiology 2022Quote: ... All stained sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) at a concentration of 1 μg/mL for 15 minutes at room temperature and mounted using ProLong Glass Antifade Mountant (ThermoFisher ...
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bioRxiv - Physiology 2022Quote: ... Tissues were counterstained with the nuclear marker 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen Cat# D1306) at a dilution of 1:1000 in diluent (Agilent Cat# S0809 ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclear staining was done by 4’,6-diamidino-2-phenylindole (DAPI, catalog # D1306, Thermo Fisher Scientific). After several washes in 1xPBS ...
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bioRxiv - Neuroscience 2022Quote: ... single nucleus suspensions were stained with DAPI (4’,6-diamidino-2-phenylindole dihydrochloride, ThermoFisher Scientific, D1306) at a concentration of 0.1μg/ml ...
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bioRxiv - Genetics 2024Quote: ... media was half-changed every 4 days with 2 µg/mL Natural Mouse Laminin (ThermoFisher Scientifics). Recordings were performed starting on day 115 using a Maestro Edge MEA system and AxIS Software Spontaneous Neural Configuration and Viability Module ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were mounted using Prolong Gold supplemented with 4’,6-diamidino-2-phenylindole (DAPI) (Life Technologies). Specimens were examined on a Zeiss Axiovert 200M microscope and images captured with an Axio-Cam MRm camera ...
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bioRxiv - Immunology 2024Quote: ... Dead cells were stained with 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, Waltham, MA) (1.0 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Counterstaining was done with 4’,6-diamidino-2-phenylindole ([DAPI], Thermo Fisher Scientific, #121101, 1µg/ml) for 15 min at RT ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Embryos older than 4 days were treated with 2 u/µl T1 RNAse (Thermo Fisher Scientific) after probe washing in order to reduce background ...