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Citations for Thermo Fisher :
2001 - 2050 of 7789 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Antibodies used were: goat anti-rat Alexa fluor 488 (1:250, A11006, ThermoFisher Scientific), goat anti-mouse Alexa fluor 568 (1:250 ...
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bioRxiv - Developmental Biology 2023Quote: ... and chicken anti-rat Alexa Fluor 647 (1:400, A-21472, Thermo Fisher Scientific). The next day ...
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bioRxiv - Neuroscience 2023Quote: ... and Ly006C+ cells were depleted using Dynabead anti-rat (Cat. #: 11035, Invitrogen Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... and Alexa Fluor 647-conjugated Donkey anti-Rat secondary antibody (A48272, Invitrogen, 1:500).
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bioRxiv - Neuroscience 2023Quote: ... we used: mouse monoclonal anti-rat pThr181 (1:1000, Invitrogen Life Technologies, Carlsbad, CA), mouse monoclonal anti-rat pSer202/pThr205 (AT8 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-rat pSer202/pThr205 (AT8, 1:1000, Invitrogen Life Technologies, Carlsbad, CA), mouse monoclonal anti-rat pThr231 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... we used: mouse monoclonal anti-rat pThr181 (1:1000, Invitrogen Life Technologies, Carlsbad, CA), mouse monoclonal anti-rat pSer202/pThr205 (AT8 ...
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bioRxiv - Neuroscience 2023Quote: ... and Donkey anti-Rat IgG Alexa Fluor 488 (1:500; Thermo Fisher; RRID: AB_2535794). Finally ...
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bioRxiv - Genetics 2023Quote: ... and goat polyclonal anti-rat Alexa Fluor 488-conjugated (Thermo Fisher Scientific; 1:250) antibodies ...
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bioRxiv - Immunology 2023Quote: ... were exposed to appropriate secondary IgG: chicken anti-rat IgG-Alexa Fluor 647 (Invitrogen), donkey anti-goat IgG-FITC (Rockland ...
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bioRxiv - Immunology 2023Quote: ... and goat anti-rat Alexa Fluor-555(Cat#A-21434, RRID:AB_141733, Invitrogen, 1:200) were used as secondary antibodies appropriately ...
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bioRxiv - Microbiology 2023Quote: ... and Alexa Fluor 546-conjugated goat anti-rat IgG (1:2000, Invitrogen, A-11081) were used as secondary antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... and AF647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:250, A15452, Thermo Fisher).
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bioRxiv - Cell Biology 2023Quote: ... or rat secondary antibodies conjugated with Alexa Fluor 488 or 555 (Thermo Fisher Scientific). Slides were mounted with antifade mounting medium and DAPI (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... goat α-rabbit and goat α-rat IgG secondary antibodies (Molecular Probes; Jackson Immunoresearch) were used at 1:500.
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bioRxiv - Neuroscience 2023Quote: ... and Ly006C+ cells were depleted using Dynabead anti-rat (Cat. #: 11035, Invitrogen Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-rat pSer202/pThr205 (AT8, 1:1000, Invitrogen Life Technologies, Carlsbad, CA), mouse monoclonal anti-rat pThr231 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... and/or anti-IgG rat (dilution 1:1000, Alexa Fluor 568; Molecular Probes, A11077). Cell nuclei were labeled with dapi (4 ’ ...
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bioRxiv - Developmental Biology 2024Quote: ... Goat anti-Rat Alexa 647 (Thermo Fisher Scientific, Catalog No. A-21247, 1/500), Donkey anti-Mouse Alexa 568 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Goat anti-Rat Alexa 568 (Thermo Fisher Scientific, Catalog No. A-11077, 1/500), and Donkey anti-Rabbit Alexa 647 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and pellets were re-suspended in Alexa Fluor 488 goat anti-rat (Invitrogen #A11006) or anti-human (Invitrogen #A11013 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... generated with an Affymetrix Rat Genome 230 2.0 array (Affymetrix, Santa Clara, CA, USA), were obtained from the Open TG-GATEs ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: Total RNA from rat cavernosum tissue was extracted by adding Trizol (Thermo Fisher Scientific), chloroform and isopropanol according to the instructions ...
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bioRxiv - Physiology 2024Quote: ... Control animals were treated with the same dosage of rat IgG isotype (Invitrogen #10700). The organs were harvested 2 or 24 hours after IR injury ...
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bioRxiv - Neuroscience 2024Quote: ... primary rat hippocampal neuronal cultures were treated with 50 nM NeutrAvidin (Thermo Fisher, #31000) in Tyrode’s solution composed of (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 405 Anti-Rat (1:300, Thermo Fisher, Cat# A48268, RRID: AB_2890549).
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor Plus 647 Anti-Rat (1:300, Thermo Fisher, Cat# A48272, RRID: AB_2893138), Alexa Fluor 488 Anti-Mouse (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 568-conjugated donkey anti-rat IgG (Thermo Fisher Scientific, #A78946, RRID:AB_2910653). The tissues were washed with PBS / 0.2% Tween-20 / 10 μg/ml heparin at 37°C for 24 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... and goat anti-rat AlexaFluor 488 (Thermo Fisher Scientific, A-11006, 1:500 dilution) secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... followed by secondary antibody Goat-α-Rat Alexa Fluor 568 (A-11077; ThermoFisher Scientific). Extensive washing steps were performed subsequent to each antibody incubation step ...
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bioRxiv - Bioengineering 2021Quote: ... and transfected a day later with 200 ng of LSR plasmid and 178 ng of pseudosite attD plasmid using Lipofectamine 2000 (Invitrogen). As a non-matching LSR control ...
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bioRxiv - Genetics 2019Quote: ... For deletions MEL745a cells were transfected with one plasmid targeting upstream and one plasmid targeting downstream sequence surrounding the enhancer using Lipofectamine 3000 (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid pCR8-pilA1-ORF was recombined with plasmid pAX1-GW(12) (Table 3) using the Gateway LR recombination protocol (Invitrogen). The resulting plasmid ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (Invitrogen). On day 6 cell culture supernatants were harvested by centrifugation of the cell culture for 30 min at 3500 rpm ...
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bioRxiv - Systems Biology 2019Quote: ... cells were co-transfected with 10 ng of pMiRCheck2 miRNA sensor plasmid and 500 ng of individual pKT2-Fah-TuDstd or pKT2-Fah-TuDtail plasmids using Lipofectamine 3000 Reagent (Invitrogen). Cultures were harvested 24 h post-transfection and processed for firefly and Renilla luciferase activity using the Dual-Luciferase Reporter Assay System (Promega).
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bioRxiv - Genetics 2019Quote: ... grown overnight and plasmid DNA was extracted using the PureLink Quick Plasmid Miniprep Kit (Thermo Fisher Scientific, Waltham, MA, USA). Plasmids were Sanger sequenced using the M13F sequencing primer and correct sgRNA sequences were identified using a BLAST alignment of the sgRNA oligos ...
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bioRxiv - Microbiology 2021Quote: ... Positive transformants were confirmed by PCR and the plasmid was extracted using a GeneJET Plasmid Miniprep Kit (Thermo Scientific™). Electrocompetent cells of Pseudomonas aeruginosa (PAO1 ...
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bioRxiv - Genomics 2021Quote: ... 1×105 cells were electropotated with 250 ng of pegRNA-encoding plasmid and 83 ng of sgRNA-encoding plasmid (for PE3) using a NEON system (ThermoFisher) at 1,050 V 30 ms (two pulses) ...
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bioRxiv - Genetics 2020Quote: The pCMV-c-JUN-MYC plasmid was generated by cloning the human c-JUN coding sequence in the pCMV-MYC plasmid (ThermoFisher). Primers used for generation of the construct are shown in supplementary table S5 ...
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bioRxiv - Neuroscience 2019Quote: ... Two million cells were transfected with 2.5 μg of each gRNA-encoding plasmid and 4 μg of donor plasmid using the Neon Transfection system (Life Technologies). A GFP puromycin-free plasmid was used as a positive control ...
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bioRxiv - Microbiology 2020Quote: ... The cloned plasmids were transfected in to HEK293T cells in combination with pSPAX2 and pCMV-G plasmids using Lipofectemine 2000 (Invitrogen). Supernatants were collected at 24 and 48 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transfected with a total of 15 μg DNA expression plasmids (7.5 μg of each plasmid in co-transfection assays) using Lipofectamine 3,000 (Thermo Fisher Scientific). After 24 h of transfection ...
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bioRxiv - Immunology 2021Quote: ... within a pEX-K4 (EtAMA1Cit) or pMK-RQ (EtIMP1Cit) plasmid with appropriate restriction enzyme sites for cloning into pYD1 yeast display plasmid vector (Invitrogen, Thermofisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were purified from 2 ml of the O/N culture using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), and sent for Sanger sequencing to DNA Sequencing and Genomics ...
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bioRxiv - Cell Biology 2020Quote: ... 15 x104 cells were harvest and co-transfected with 0.1 µg control plasmid Renilla and 5µg reporter plasmid Firefly (Neg or Mut) via electroporation using Neon™ Transfection System (MPK1096, Invitrogen) with following parameters ...
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bioRxiv - Cancer Biology 2021Quote: ... In transient transfection of HEK293FT cells with pRK5-TGFβRI-FLAG plasmid (#14833) or pCMV5B-TGFβRII plasmid (#11766) using Lipofectamine 3000 transfection kit (Invitrogen, #L3000-008). For lentivirus production ...
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bioRxiv - Cancer Biology 2022Quote: ... the cloned plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Invitrogen) based on the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Gene editing of pig fibroblast cells were performed by electroporation of wildtype large white pig fibroblast cells with a plasmid encoding Piggybac transposase and pBv1-EF-6X plasmid using Neon Transfection System (Invitrogen) under condition of 1450 V ...
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bioRxiv - Cancer Biology 2022Quote: H-1299 and H-23 cells were transfected with plasmids or co-transfected with plasmids and siRNAs using Lipofectamine 2000 (Invitrogen) as in the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: HEK293FT cells were transfected with the plasmid mix (plasmid of interest, PPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher) as a transfection reagent diluted in Opti-MEM (Thermo Fischer) ...