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Citations for Thermo Fisher :
1901 - 1950 of 7789 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1.25 μg of each pcDNA5/FRT/TO/i/FLAG/MCP-based plasmid and 1.25 μg of pOG44 plasmid encoding recombinase were transfected using Lipofectamine LTX (Invitrogen). Cell lines with stably integrated constructs were selected with 150 μg/ml hygromycin (Wako) ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µg packaging plasmid (psPAX2) and 2.5 µg envelope plasmid (pMD2.G) using Lipofectamine 2000 in OptiMEM (ThermoFisher Scientific). After 6-hr incubation of Lipofectamine/DNA mixture in OptiMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was isolated by the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit (Thermo Fisher Scientific, Waltham, MA), and precipitated onto gold particles (0.6 µm ...
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bioRxiv - Biophysics 2023Quote: ... with 10-100 ng of the K2P2.1 plasmid and 400 ng of an eGFP plasmid (for visualization) using LipofectAMINE 2000 (Invitrogen) for 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... CRISPR plasmids and pEGFP-C1 puromycin plasmids were co-transfected in HeLa cells using lipofectamine LTX reagent (ThermoFisher, 15338030), according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... To standardize the mass of transfected plasmids among transfection experimental conditions we added a filler pcDNA3.1 “empty” plasmid (Thermofisher).
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bioRxiv - Molecular Biology 2023Quote: ... the cells were co-transfected with 0.5 μg of the corresponding pcDNA5 plasmid and 4.5 μg recombinase plasmid POG44 (Invitrogen V600520). After 48 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.84 μg of pCMV-VSV-G envelope plasmid, 5.67 μg of pCMV dR8.2 dvpr packaging plasmid in OptiMEM medium, Gibco #31985062) and 1 ml of premix 2 (42.5 μl of Lipofectamine LTX ...
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bioRxiv - Bioengineering 2024Quote: ... For endogenous RNA trans-splicing experiments 400 ng of dCas13 expression plasmid was mixed with 400 ng of rcRNA expression plasmid in 50µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific). In a second tube ...
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bioRxiv - Bioengineering 2024Quote: ... 300 ng of dCas13 expression plasmid was mixed with 300 ng of rcRNA expression plasmid and 300 ng of splitGFP reporter plasmid in 50µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific). In a second tube 3.2 µl of polyethylenimine (Polysciences ...
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bioRxiv - Cancer Biology 2021Quote: ... or 50 μg/mL neutralized rat tail collagen (rich in Coll I) (Gibco, A1048301) for 2 hours to overnight at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... and secondary antibodies (1:400, Alexa Fluor 633 goat anti-rat IgG, Thermo Fisher Scientific # A21094 ...
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SIRT1 ameliorates premature senescence-induced defenestration in hepatic sinusoidal endothelial cellbioRxiv - Cell Biology 2020Quote: ... Primary rat HSECs were cultured in plates with medium comprising 80% MCDB131 (Gibco, 10372019) and 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rat Alexa Fluor 647 from goat (A21247, Invitrogen, at 1:1000 for IF), peroxidase-conjugated anti-mouse from goat (115-035-003 ...
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-rat IgG Alexa Fluor 488 (A11006) were purchased from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2022Quote: ... The rat brains were removed and placed in ice-cold dissection media: HBSS (Gibco) supplemented with 30mM glucose ...
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bioRxiv - Cancer Biology 2022Quote: ... and Alexa Fluor® 546 goat anti-rat (dilution 1/200, Invitrogen, cat. A11081) was used as the secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... Larval fillet preparations were fixed using formaldehyde and stained with rat anti-mCD8 (ThermoFisher) at 1:100 followed by Alexa Fluor 488 anti-rat secondary antibody (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: ... and subsequent incubation with donkey anti-rat Alexa Fluor 555-conjugated secondary antibody (ThermoFisher) at a 1:500 dilution for 30 min on ice ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... GR1 and MAC1 in combination with sheep anti-rat IgG Dynabeads (Thermo Fisher Scientific) prior to the specific antibody staining ...
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bioRxiv - Immunology 2019Quote: ... Secondary antibodies goat anti-rat AlexaFluor® 594 (1:200) (#A-11007, Molecular Probes), goat anti-rabbit AlexaFluor®633 (#A-21071 ...
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bioRxiv - Microbiology 2020Quote: ... and goat anti-rat IgG-Alexa 488 (1:2000 dilution, Thermo Fisher, A-11006). To verify the absence of the MYR1 protein ...
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bioRxiv - Developmental Biology 2019Quote: ... and a goat anti-rat Alexafluor-647-conjugated secondary antibody (Invitrogen, # A21247, 1:200) was used to detect the anti-CD31 primary antibody ...
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bioRxiv - Cell Biology 2019Quote: ... or anti-rat secondary antibody conjugated with HRP (1:10000, Invitrogen, #18-4818-82) for 1h at RT ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in secondary donkey anti-rat ALEXA 594 (Invitrogen, Burlington, ON, Canada) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Alexa Fluor 488-conjugated rat anti-PNAd (Thermo Fisher, 53-6036-82, 1:50)
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488 conjugated chicken anti-rat (1:400, Invitrogen, Grand Island, NY, USA) Alexa Fluor 568 conjugated goat anti-rabbit (1:400 ...
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bioRxiv - Pathology 2019Quote: ... sections were then incubated with either rat anti-F4/80 (Invitrogen, cat. number MF48000) (1:1000 in blocking buffer TBS with 2% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... together with Alexa Fluor 555 goat anti-rat IgG (Invitrogen #A-21434, 1:3000) for 1 hour at room temperature and mounted using ProLong Gold with Dapi (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... a rat monoclonal antibody against yeast alpha-tubulin (clone YL1/2) from Thermo Fisher Scientific (Pittsburgh ...
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bioRxiv - Bioengineering 2021Quote: ... A rat-specific primary antibody for skeletal muscle myosin heavy chain (MA1-35718, ThermoFisher) was diluted 1:5 in TBS-NGS ...
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bioRxiv - Neuroscience 2021Quote: ... the newborn rat hippocampi (< 24 h) were chopped and digested by 0.25% trypsin (Gibco) for 8 min in 5% CO2 incubator at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... stained again in 100 μl buffer with the anti-rat AlexaFluor488 (ThermoFisher Scientific, #A11006) secondary antibody ...
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bioRxiv - Cancer Biology 2020Quote: 3D culture was performed using rat tail Collagen I (Corning®, Fisher Scientific, France). The collagen I solution was neutralized using 1N NaOH and diluted in PBS to a final concentration of 1mg/ml for the contractility assay ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in blocking solution and secondary Alexa594 goat anti-rat IgG antibodies and DAPI (Invitrogen) in blocking solution for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... rat astrocytes were plated at 6-8K per well in pre-warmed DMEM (Invitrogen), supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2021Quote: ... diluted to 1:125 followed by Alexa 488-conjugated anti-rat antibody (Molecular probes) diluted to 1:250 were used ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa-568 Goat anti-Rat (1:500; A-11077, Thermo Fisher Scientific, Reinach, Switzerland). DAPI (4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Immunology 2022Quote: ... and Lin+ cells were depleted with sheep anti-rat IgG immunomagnetic beads (Dynabeads, Invitrogen). Cells were then immunostained and Lin- Flk2+CD27+ BM progenitors from F1 congenic strains were FACS purified (Fig ...
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bioRxiv - Developmental Biology 2022Quote: ... Rat monoclonal anti tyrosinated tubulin YL1/2 was diluted 1:500 (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... a mixture of rat tail collagen type I (Cultrex) and Matrigel™ (Life Technologies) was mixed with CMs and gamma-irradiated human foreskin fibroblasts (HFFs ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were Alexa Fluor®-488 goat anti-rat (Thermo Fisher Scientific™); Alexa Fluor®-647 goat anti-rat (Thermo Fisher Scientific™) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor 555 goat anti-Rat IgG antibody (A21434) was purchased from Life Technologies. Hematoxylin (G1004 ...
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bioRxiv - Neuroscience 2020Quote: ... with goat anti-rat (1:500; A-11006, RRID:AB_2534074, Thermo Fisher Scientific, Waltham, MA), goat anti-rabbit (1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa Fluor 555 anti-rat (1:200, Thermo Fisher, cat# A-21434, RRID: AB_2535855) or Alexa Fluor 555 anti-rabbit (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Cotton rat lung epithelial cells (CRLC, ATCC PTA-3930) were grown in MEM (Gibco) supplemented as above except with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... and Alexa Fluor 594 donkey anti-rat (1:500 IgG; Life Technologies, A-21209) in 2% NDS and PBS-T for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-rat IgG (H+L) cross-adsorbed (Alexa Fluor 488, Life Technologies, A1106) and goat anti-mouse IgG2b cross-adsorbed (Alexa Fluor 488 ...
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bioRxiv - Cell Biology 2021Quote: ... goat anti-rat IgG (H+L) Alexa Fluor 555 conjugate (Molecular Probes, 1:1000), Cy3 donkey anti-mouse IgG (H+L ...
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bioRxiv - Biochemistry 2021Quote: ... and rabbit anti-rat IgG biotin-SP (31834, 1:200, Thermo Scientific, Rockford, IL), respectively ...