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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: Rabbit polyclonal anti-Human ASGR1 antibody (Thermo Fisher, PA5-80356), 1:1,000 ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Gibco Human Episomal iPSC clone (Control 1, Thermo Fisher Scientific) and CW30318 (Control 2 ...
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bioRxiv - Microbiology 2023Quote: ... HRP labelled goat anti-human IgE antibodies (Invitrogen, Waltham, MA) were used as the secondary antibodies for detection and the color wasa developed using TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Human female embryonic kidney HEK293FT cells (#R70007, Invitrogen; RRID: CVCL_6911), immortalized mouse embryonic fibroblasts (MEFs) ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Anti-human CD45-PerCP-Cy5.5 (45-9459-42, Affymetrix eBioscience), CD45-APC Cy7 (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... They were cultured on human recombinant vitronectin (Thermo Fisher Scientific) coated plates in StemFlex medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Human foreskin fibroblasts (HFFs) were maintained in MEM (Gibco/BRL) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2024Quote: ... and stained with mouse anti-human CD20 (PE-Cy5.5; Invitrogen) and anti-human IgG Abs (PE-Cy7 clone G18-145 ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A ...
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bioRxiv - Developmental Biology 2024Quote: KOLF2 human iPSCs were maintained in E8 flex media (ThermoFisher) and manually dissociated using Gentle Cell Dissociation Reagent (STEMCELL Technologies ...
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bioRxiv - Immunology 2024Quote: ... and stimulated with human CD3/CD28 Dynabeads (Life Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and OxPhos Human WB Antibody Cocktail (Thermo Fisher 45-8199). Detection of protein signals was performed using ECL Prime Western Blotting Detection Reagent (Cytiva RPN2236 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK 293FT human embryonic kidney cells (Thermo Fisher Scientific, #R70007) were cultured in DMEM (Corning ...
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bioRxiv - Physiology 2024Quote: ... Human neutrophils were cultured in RPMI-1640 media (Gibco, #52400017) + 10% FCS + 1% penicillin/streptomycin and the purity of isolated neutrophils was confirmed as >98% CD45+ CD15+ via flow cytometry (anti-CD45-FITC ...
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bioRxiv - Systems Biology 2024Quote: ... FGF Basic (aa 10 155) Recombinant Human Protein (Gibco #PHG0021), 1% Penicillin-Streptomycin Solution and 1% L-glutamine ...
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bioRxiv - Molecular Biology 2024Quote: ... and human HEK293T cells was extracted using TRIzol reagent (Invitrogen). Endogenous RNase was further removed by ethanol precipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... LAN5 human neuroblastoma cells were cultured in RPMI (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... IL-2 was measured by human IL-2 ELISA (Invitrogen) using the manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... human H2BC1 and H2AZ2 cDNA were synthesized by Thermo Fisher and cloned into the pCW-Cas9 vector (Addgene #50661 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with Human CD11b-APC conjugate ( Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... or human TGFβ1 recombinant protein (10ng/mL)(ThermoFisher Scientific, PHG9204) for 24 hours followed by addition of lipopolysaccharides (LPS ...
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Intranasal Delivery of shRNA to Knockdown the 5HT-2A receptor Enhances Memory and Alleviates AnxietybioRxiv - Neuroscience 2023Quote: ... we chose the human specific 5HT-2A antibody from ThermoFisher PA5-120747 at 1:50 with secondary Goat Anti-Rabbit Fluor555 at 1:1000 using a MOI of 3 ×105 ...
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bioRxiv - Neuroscience 2023Quote: The IL-6 Receptor (Soluble) Human ELISA Kit (Invitrogen; BMS214) was used to quantify soluble IL-6Ra expression in cell culture media ...
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bioRxiv - Immunology 2024Quote: ... anti-human CD45 (clone: MEM-28) conjugated to AF488 (ThermoFisher) was used ...
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bioRxiv - Cell Biology 2024Quote: ... and human embryonic kidney 293T (ATCC) were grown in (Invitrogen) supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Stimulation with αCD3/CD28 DynaBeads Human T cell activator (Invitrogen) (cell to bead ratio of 1:1 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by anti-human IgG–Alexa594 (1:1000, ThermoFisher Scientific) for 30 min and analyzed by flow cytometry ...
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bioRxiv - Biophysics 2023Quote: ... were cultured in human endothelial serum-free medium (SFM, Gibco) containing 20% foetal bovine serum ...
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bioRxiv - Molecular Biology 2024Quote: Human neonatal epidermal melanocytes (HEMn-DP, Thermo Fisher Scientific, C2025C) were maintained in Medium 254 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... The following day cells were co-transfected with 2 µg of Fluc plasmid DNA and 20 ng of Rluc control plasmid (pRL) per well using Lipofectamine 2000 (Thermo Fisher, 116688-019) and incubated for 16 h at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T/17 cells at sub-confluence in 6-well plates or 100 mm dishes were transfected with HLA-DRA plasmid or HLA-DRB1 plasmid or a 1:1 combination of both using the Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific, UK) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2019Quote: ... lentivector plasmids were transfected into HEK293T cells together with packaging and envelope plasmids (psPAX2 and pMD2.G) using Lipofectamine 3000 (Thermo Fisher Scientific, L3000008). Three days after transfection ...
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bioRxiv - Cell Biology 2021Quote: The human MISP sequence harbored in a pCMV-SPORT plasmid (Harvard PlasmID Database; HsCD00326629) was subcloned by PCR and TOPOcloned into a pCR™8 Gateway entry vector (Invitrogen; 46-0899). In-frame sequence insertion was confirmed by sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... For lentivirus particle production these plasmids were recombined with pCDH-EF1a-Gateway-IRES-BLAST plasmids as described in [51] using LR Clonase II (Invitrogen/Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... LINC000881 overexpression was achieved by lipofectamine based transfection of LINC00881 plasmid versus bacterial alkaline phosphatase control plasmid using RNAiMAX reagent (ThermoFisher Scientific, Waltham, MA). iPSCs were harvested at 48-72 hours following transfection for functional and gene expression studies ...
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bioRxiv - Microbiology 2021Quote: ... jejuni PglB was amplified by PCR from the plasmid pACYCpgl with the pTac promoter and LacIq repressor from plasmid pEXT20 as a template using Pfx Polymerase (Thermo Fisher Scientific UK) with (SEQ ID 15 ...
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bioRxiv - Immunology 2022Quote: ... The cells were co-transfected with 650 μg of SOSIP expressing plasmid DNA and 150 μg of furin expressing plasmid DNA complexed with 293Fectin (ThermoFisher Cat No. 12347019). On day 6 cell culture supernatants were harvested by centrifugation of the cell culture for 30 min at 3500 rpm ...
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bioRxiv - Molecular Biology 2019Quote: To generate linear plasmids without telomeric ends, the circular plasmids were amplified with primers (BP1204, BP1205; Table 2) using Phusion polymerase (Thermo Fisher Scientific, USA). Cycling conditions were ...
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bioRxiv - Synthetic Biology 2022Quote: ... we developed a vacuum-based plasmid extraction protocol named “TeVacS” on Fluent using the PureLink™ Pro Quick96 plasmid purification Kit (K211004A) (Invitrogen, Carlsbad, CA). Our customized protocol features the use of vacuum manifold for efficient removal of washing buffer and final elution of plasmid DNA into a 96-well plate placed on a 3D-printed holder ...
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bioRxiv - Developmental Biology 2022Quote: ... 90 ng of ASD library plasmids and 30 ng of control library plasmids were used for electroporation of MegaX DH10B T1R Electrocomp™ Cells (Thermofisher, cat.no. C640003) following the manufacturer’s guide ...
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bioRxiv - Physiology 2022Quote: ... HPAECs were transfected with either control plasmids (empty vector) or 1μg of HA-ubiquitin plasmids (HA-tagged Ubi) using Lipofectamine 3000 (Thermo fisher Scientific, PA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... a TMPRSS2 plasmid and S plasmids from SARS-CoV-2 variants into 293T cells using Lipofectamine 3000 transfection reagent (L3000-001, ThermoFisher Scientific, Asheville, NC) 39,40 ...
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bioRxiv - Microbiology 2022Quote: ... we isolated fresh plasmid DNA for use in transfection experiments by using the PureLinkTM HiPure Plasmid Maxiprep Kit (Invitrogen, Thermo Fisher Scientific Corp.). This method minimizes lipopolysaccharide contamination in plasmid DNA extracted from Escherichia coli and reduces non-specific PRR pathway activation in plasmid DNA transfections of mammalian cells ...
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bioRxiv - Microbiology 2022Quote: ... we isolated fresh plasmid DNA for use in transfection experiments by using the PureLinkTM HiPure Plasmid Maxiprep Kit (Invitrogen, Thermo Fisher Scientific Corp.). This method minimizes lipopolysaccharide contamination in plasmid DNA extracted from Escherichia coli and reduces non-specific PRR pathway activation in plasmid DNA transfections of mammalian cells ...
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bioRxiv - Biophysics 2022Quote: ... 2 μg total plasmid DNA (725 ng CagFbFP-Q148V plasmid + 1275 mg MTS-CagFbFP plasmid) and 2 μl PLUS Reagent were diluted in 100 μl Opti-MEM (Thermo Fisher Scientific, USA), and 4 μl Lipofectamine LTX were diluted in 100 μl Opti-MEM ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cells were transfected by transfer plasmid and packaging plasmids psPAX2 (addgene #12260) and pMD2.G (addgene #12259) using Lipofectamine 3000 reagent (Thermo Fisher Scientific # L3000015). Viral particles were concentrated using Lenti-X Concentrator (Takara Bio ...
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bioRxiv - Biochemistry 2023Quote: ... Both pDEST-CMV-N-EGFP-wtG3BP2 and pDEST-CMV-N-EGFP-C73SG3BP2 plasmids were amplified and extracted using PureLink™ HiPure Plasmid Maxiprep Kit (Invitrogen™, K210007) before use.
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bioRxiv - Genetics 2024Quote: ... A subset of the saturated culture was used to extract purified plasmid DNA using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, K0503).
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...