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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies against the indicated antigen were purchased from Thermo Fisher Scientific and were used at 1 μg/ml unless stated otherwise ...
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bioRxiv - Immunology 2021Quote: ... The whole viral antigen was coated in the 96 well plate (Nunc) using bicarbonate coating buffer (pH=9.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... Antigens were mixed with an equal volume of Imject alum adjuvant (Thermofisher) and administered three times ...
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bioRxiv - Immunology 2021Quote: ... The following antigens were coupled with carboxylated beads (Luminex Corp): BSA (Affymetrix), goat anti-mouse Igκ ...
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bioRxiv - Physiology 2023Quote: ... and targeted antigen retrieval performed using boiling 1x Targeted Retrieval Solution (Invitrogen) for 20 mins ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antigens were coupled to Neutravidin yellow-green fluorescent beads (Thermo Fisher). Antigen-coupled beads were incubated with 1:500 diluted serum samples for two hours at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The following antigens were coupled with carboxylated beads (Luminex Corp): BSA (Affymetrix), goat anti-mouse Igκ ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated antigen was then added to FITC-conjugated neutravidin beads (ThermoFisher, F8776) at an antigen(μg):bead(μL ...
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bioRxiv - Cancer Biology 2023Quote: ... After treating them with IHC Antigen Retrieval Solution (00-4956-58, Invitrogen), the sections were stained with an anti-human CD4 Ab that was pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Immunology 2024Quote: Antigens were biotinylated using EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher) per manufacturer’s instructions and coupled to streptavidin beads (NEB) ...
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bioRxiv - Physiology 2024Quote: ... Antigen retrieval was performed using 10mM Sodium Citrate (Fisher Scientific, BP327-500) with 0.05% Tween 20 (Calbiochem ...
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bioRxiv - Neuroscience 2024Quote: ... Microwave antigen retrieval with 10 mM sodium citrate (Fisher Scientific S279-3), pH 6.0 ...
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bioRxiv - Systems Biology 2019Quote: ... using a 3-45 % acetonitrile gradient over 60 min and analyzed online by nanoelectrospray-ionization tandem mass spectrometry on an Orbitrap Fusion or Orbitrap Fusion Lumos Tribrid (Thermo Scientific). Data-dependent acquisition was activated ...
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bioRxiv - Systems Biology 2020Quote: ... using a 3-45 % acetonitrile gradient over 60 min and analyzed online by nanoelectrospray-ionization tandem mass spectrometry on an Orbitrap Fusion or Orbitrap Fusion Lumos Tribrid (Thermo Scientific). Data-dependent acquisition was activated ...
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bioRxiv - Microbiology 2020Quote: ... truncated P1 adapters at 3′-region were generated by nested RT-PCR with a fusion method according to the manufacturer’s instructions (Fusion methods from Ion Torrent; Thermo Fisher Scientific). Information on the fusion PCR primers is available upon request ...
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bioRxiv - Plant Biology 2021Quote: ... or GLK2 was recombined into the destination vector pGWB605 for C-terminal fusion with sGFP or into pGWB617 for C-terminal fusion with 4×Myc through the Gateway LR reaction (Thermo Scientific). For the 35S:GLK1-4×Myc (or sGFP ...
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bioRxiv - Plant Biology 2019Quote: ... or the stop codon-less full CDS of PNPAR was recombined into the destination vector pGWB651 for C-terminal fusion with GFP or pGWB617 for C-terminal fusion with 4xMYC through Gateway LR reaction (Thermo Scientific) to create p35S:tPNP-A-GFP and p35S:PNPAR-4xMYC ...
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bioRxiv - Microbiology 2024Quote: ... a combination of two techniques was employed – Liquid Chromatography analysis in connection with Orbitrap Fusion mass spectrometer (LC-MS/MS; Orbitrap Fusion, Q-OT-qIT, Thermo Scientific) and two-dimensional comprehensive gas chromatography in connection with mass spectrometer (GCxGC-MS ...
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bioRxiv - Biochemistry 2021Quote: ... The FL-NP construct included a C-tag peptide (EPEA) at the C-terminus (27) for affinity chromatography purification using a C-tag affinity resin (Thermo Fisher Scientific), eluting with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
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bioRxiv - Biochemistry 2020Quote: ... 3ul of anhydrous ACN and 4 ul of TMT reagent (32ug of TMT reporter tag) of each TMT reporter tag (TMT 10 plex kit, Product number: 90110 and A37725., Thermo Fisher Scientific) was added to each sample and incubated at room temperature on a Thermo mixer with a gentle agitation (600 rpm ...
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bioRxiv - Microbiology 2021Quote: ... Permeabilized cells were probed with a monoclonal antibody against the HA-tag epitope (1:1000; HA Tag mAb 2-2.2.14, Thermo Fisher Scientific, Planegg, Germany) to detect SARS-2-S protein ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 ng/ml recombinant mouse EGF and 100 ng/ml recombinant murine Noggin (all from Thermo Fisher Scientific). Organoids were passaged 3 times a week depending on culture confluence.
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bioRxiv - Cancer Biology 2021Quote: ... FC-1242, and FC-1245)(Engle et al., 2019) were cultured in 10% FBS (Biowest) and 1% Anti-Anti (Gibco) in DMEM (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 2 % FCS/PBS and then re-suspended in 2 % FCS/PBS containing 7-amino actinomycin (7-AAD, Invitrogen) at a concentration of 5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Undigested IgG and Fc fragments were removed by a 2 h incubation with CaptureSelect IgG-Fc resin (Thermo Fisher Scientific). Resin was spun down and the supernatant run on a Superdex 200 increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and enzyme activity was quenched by addition of FCS followed complete DMEM medium (supplemented with 10% FCS, 2 mM L-glutamine (ThermoFisher) and 10000 U/mL penicillin/streptomycin (ThermoFisher)) ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463; IgG Fc Rabbit anti-Mouse, HRP, Invitrogen 31455 ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were prepared in serum-free VP-SF media (Invitrogen). Viruses were harvested before CPE development ...
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bioRxiv - Cell Biology 2021Quote: ... The virus packaging was performed in HEK293FT cells (Thermo Fisher Scientific) based on a calcium precipitation method using pUMVC and pCMV-VSV-G vectors (37 ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). According to the manufacturer’s instruction (QIAamp Viral RNA mini kit (Qiagen)) ...
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bioRxiv - Microbiology 2022Quote: ... using TaqMan Fast Virus 1-Step Master Mix chemistry (Applied Biosystems). SARS-CoV-2 genomic RNA was amplified and detected using forward (5’- CGTGTAGTCTTTAATGGTGTTTCC-3’ ...
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bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... TaqMan™ Fast Virus 1-Step Master Mix (4444432, Life Technologies) was used for one-step RT-qPCR and TaqMan Fast Advanced Master Mix (4444556 ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were treated with 40 U/mL DNase I (Invitrogen) for 1 hr at 37°C before they were used to infect cells for 2 hr at an MOI of 0.5-2 ...
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bioRxiv - Physiology 2022Quote: ... and 200 U of Moloney murine leukemia virus reverse transcriptase (Invitrogen) in a final reaction volume of 20 μL (Evans-Molina et al. ...
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bioRxiv - Microbiology 2020Quote: ... using TaqMan Fast Virus 1-Step Master Mix chemistry (Applied Biosystems). SARS-CoV-2 N gene RNA was amplified using forward (5’-GACCCCAAAATCAGCGAAAT ...
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bioRxiv - Genetics 2020Quote: ... Cells were then transduced using CytoTune-iPS 2.0 Sendai virus (Invitrogen) as outlined in the supplier’s protocol at an MOI of 5:5:3 (KOS [Klf4 ...
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bioRxiv - Microbiology 2021Quote: Virus replication assays were performed in RPMI 1640 (Gibco 11875-093) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... 5 μl of TaqMan Fast Virus 1-Step Master Mix (ThermoFisher), and 9 μl of molecular-grade water per reaction ...
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bioRxiv - Immunology 2023Quote: ... Taqman Fast Virus 1-step kit (Thermo Fisher; catalog number 4444434) with oligos and probe specific to the N gene of SARS-CoV2 was used for SARS-CoV-2 RNA detection according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat No. 5555532) and a commercially prepared RNAse P primer/probe combination (IDT ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... dNTP and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) were used to synthesize cDNA ...
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bioRxiv - Molecular Biology 2023Quote: Virus particles were fixed in 1 % glutaraldehyde (Cat# 233281000, Thermo Scientific). A 4 µL aliquot of sample was adsorbed onto holey carbon-coated grid (Lacey ...
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bioRxiv - Immunology 2019Quote: ... the wild-type and mutant (C302/305R) recombinant Mul1 proteins were produced in Sf9 cells using the Bac-to-Bac Baculovirus expression system (Invitrogen, Carlsbad, CA, USA) and purified by a nickel-chelating resin (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... The same recombinant Cdk5/p25 protein used for the MST assays was labeled with biotin using EZ-LinkNHS-PEG4-Biotinylation Kit (Thermo Scientific, Waltham, MA) and excess reagent was removed with a spin desalting column as per the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...