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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and a MegaScript T7 transcription kit (Thermo Fisher Scientific, Waltham, MA, USA) were used ...
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bioRxiv - Neuroscience 2023Quote: ... cRNAs were produced using the mMESSAGE mMACHINE T7 kit (Applied Biosystems/Ambion). The capped mRNAs were then purified using the MEGAclear kit (Applied Biosystem/Ambion) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... mRNA was synthesized with the mMESSAGE mMACHINE T7 Transcription Kit (AM1344, Ambion) and eluted in RNAse-free water ...
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bioRxiv - Plant Biology 2023Quote: dsRNA was produced in-vitro with MegaScript T7 Transcription Kit Plus (ThermoFisher). Sequences are shown in Table S1 ...
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bioRxiv - Neuroscience 2023Quote: ... cRNAs were produced using the mMESSAGE mMACHINE T7 kit (Applied Biosystems/Ambion). The capped mRNAs were then purified using the MEGAclear kit (Applied Biosystem/Ambion) ...
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bioRxiv - Genomics 2023Quote: ... TALEN mRNAs were prepared using a mMessageMachine T7 Ultra Kit (#AM1345, Ambion). Sense and anti-sense TALENs recognize the sequences are presented in Supplementary table 3.
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bioRxiv - Molecular Biology 2023Quote: T7 promoter-containing plasmid DNA was digested with FastDigest MvaI (Thermo Scientific) and used as template for in vitro transcription of tRNA using the AmpliScribe T7-Flash Transcription Kit for 4 h at 42°C (see Table S3) ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were then synthesized using the T7 or Sp6 Megascript Kit (Invitrogen) and DIG RNA labeling mix (Roche ...
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bioRxiv - Biophysics 2023Quote: ... probes were in vitro transcribed with the MEGAshortscript T7 kit (ThermoFisher AM1354) for 4 h at 37 °C and purified with RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... expressing Cas9 and T7 RNA polymerase8 were grown in M199 (Life Technologies) supplemented with 2.2 g/L NaHCO3 ...
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bioRxiv - Biophysics 2024Quote: ... and cRNA was synthesized using mMESSAGE mMACHINE T7 Transcription kit (Invitrogen Ambion).
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bioRxiv - Biophysics 2024Quote: ... and cRNA was synthesized using mMESSAGE mMACHINE T7 Transcription kit (Invitrogen Ambion).
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bioRxiv - Cell Biology 2024Quote: ... followed by reverse transcription using the MEGAScript T7 transcription kit (Life Technologies) according to the manufacturer’s instructions (Table S4) ...
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bioRxiv - Cell Biology 2024Quote: ... Cas9 mRNA was in vitro transcribed with mMESSAGE T7 Ultra Kit (Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and in vitro transcription performed (MEGAscript T7 Transcription Kit, Thermo Fisher Scientific) during 16 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was transcribed in vitro (MEGAscript T7 transcription kit – Thermo Fisher AM1334), and 10 µg of purified RNA was immobilized on streptavidin magnetic beads (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... purified protein was digested with TEV protease and then further purified by size-exclusion chromatography using Superdex 200 Increase 10/300 GL (Thermo Fisher Scientific) in 0.2 M NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... and ‘superfolder’ GFP followed by a TEV-protease cleavage site) by transient transfection of HEK293F suspension cultures.3 Suspension culture FreeStyle HEK293F cells (Thermo Fisher Scientific) were transfected as previously described.4 Six days post transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... Genes were cloned into modified pRSF1-Duet expression vector with a TEV-cleavable N-terminal 14-histidine tag (39) using New England Biolabs and Anza (Thermo Fisher Scientific) enzymes ...
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bioRxiv - Cancer Biology 2021Quote: ... The HA-tagged cDNAs were subcloned into the pLenti6-D-TOPO vector (Invitrogen). The cDNA for human GSK3βwas subcloned into the pCMV- myc vector ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by the Alexa-tagged secondary antibodies (Alexa 488; A21206, Invitrogen) diluted in 7% serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: ... we performed bilateral injections of fluorescently-tagged polystyrene microspheres (10 micron, Invitrogen F8836) into the anterior chambers of 6-8 weeks old mice (Calkins et al. ...
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bioRxiv - Cell Biology 2021Quote: ... LDs were labeled by BODIPYTM 558/568 (FA568, fluorescent tagged oleic acid, Thermofisher) or LD540 (Spandl et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Cholera toxin subunit B (CTXβ) tagged with Alexa 555 or 647 (Molecular Probes) for histology experiments.
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent detection was done with an Alexa- tagged secondary antibody from Molecular Probes, donkey anti-sheep (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Assay mixes with 1 nM GST-tagged GR (aa 521-777, Invitrogen #PV4689), 25 nM Alexa-488 conjugated GST antibody (Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... The V5-AID tagged Brn1 was detected with anti-V5 antibody (Invitrogen, R96025) at a 1:4000 dilution ...
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bioRxiv - Bioengineering 2019Quote: ... one-hour incubation of Alexa 568 tagged secondary antibody (Fisher Scientific, Hampton, NH) performed and stained with DAPI (Vecor Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were tagged using the Cell Trace Far Red dye (ThermoFisher Scientific) prior to injection into the slices ...
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bioRxiv - Microbiology 2021Quote: ... followed by fluorescence tagged goat anti-rabbit IgG Alexafluor 488 (1:200, Invitrogen) and fluorescence tagged goat anti-mouse IgG Alexafluor 594 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa 555 or Alexa 594 tagged secondary antibodies (Invitrogen, Germany, dilution 1:2000) were used ...
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bioRxiv - Neuroscience 2022Quote: ... and Alexa Fluor 647-tagged donkey anti-rabbit IgG (1:500, Thermo Fisher, catalog # A-31573 ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa 488 or Alexa 594 tagged secondary antibodies (Invitrogen, Germany, dilution 1:1000) were used ...
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bioRxiv - Microbiology 2023Quote: ... then incubated with Alexa-488 tagged secondary antibody (goat anti-mouse IgG, Invitrogen) at a dilution of 1:500 ...
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bioRxiv - Biophysics 2023Quote: ... After incubation with appropriate HRP-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher), the blots were imaged and processed with a FlourChem E (Protein Simple) ...
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bioRxiv - Cell Biology 2023Quote: ... or S1S2 constructs tagged with FLAG epitope were transfected using Lipofectamine 3000 (Invitrogen) as described previously [11].
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bioRxiv - Developmental Biology 2023Quote: ... cells/sections were treated with fluorescence (Cy3/FITC)-tagged secondary antibodies (Life Technologies) diluted in 5% donkey serum and 0.2%/0.4% Triton X-100 solution for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... After incubation with appropriate HRP-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher), or purified LgBiT-substrate cocktail (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... with Flag-tagged siRNA resistant TDP-43 or pRc/CMV control vector (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... these gels were treated with alexa-fluor tagged secondary antibodies and streptavidin (Invitrogen) and then imaged under a fluorescent inverted microscope (Olympus ...
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bioRxiv - Developmental Biology 2021Quote: ... recombinant murine EGF (50 ng/ml; Thermo Fisher Scientific), recombinant murine Noggin (100 ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... including 1.25 U Taq recombinant DNA polymerase (Invitrogen, USA), 1x PCR Buffer (-Mg) ...
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bioRxiv - Molecular Biology 2021Quote: ... recombinant murine EGF (50 ng/mL; Thermo Fisher Scientific), recombinant murine Noggin (50 ng/mL ...
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bioRxiv - Genomics 2020Quote: ... treated with RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific). Library concentrations were determined using Qubit Fluorometer (v.4.0 ...
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bioRxiv - Genomics 2020Quote: ... treated with RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific). Library concentration was determined using Qubit Fluorometer (v.4.0 ...
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bioRxiv - Cell Biology 2021Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (ThermoFisher Scientific # 10777019) according to manufactures instructions ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgG Fab recombinant secondary antibody (Invitrogen), anti-NKp46 (9E2 ...
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bioRxiv - Genetics 2021Quote: ... Extracted RNA was treated with Recombinant DNaseI (Thermo Scientific) for 30 min at 37°C ...
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bioRxiv - Genetics 2022Quote: ... and 200 μg/mL recombinant proteinase K (Thermo Scientific). The mixture was incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 40U RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen, Antisel SA, Hellas) and 4 μM random hexamer primers (Takara Bio ...