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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and inserted into similarly digested pcDNA4-To/myc-His B (Invitrogen), resulting in pcDNA4-L3HYPDH ...
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bioRxiv - Microbiology 2023Quote: ... 10% heat-inactivated fetal bovine serum (HI-FBS; Hyclone and Gibco), and 100 U/mL penicillin/streptomycin (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken for immunoblotting using anti His-tag antibody (ThermoFisher). Crosslinking and control reactions intended for scramblase activity assays were stopped with 1 M Tris pH 7 to a final concentration of 10 mM ...
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bioRxiv - Biochemistry 2023Quote: ... All media was supplemented with 10% HI FBS (Gibco cat # 10438026).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FBS (HI-FBS; Gibco). Immortalised adult human C20 microglial cells were kindly provided by Dr David Alvarez Carbonell ...
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bioRxiv - Biochemistry 2023Quote: ... Hi Five cells grown in Sf-900 II SFM media (Gibco) at a density of 2.0 × 106 cells/mL were infected with baculovirus at 1.5% v/v ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with Novex Hi-Density TBE Sample buffer (ThermoFisher Scientific, LC6678) and analysed on 10% Novex TBE gels (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... His-HcpGA1 was purified using the ProBond Purification System (Life Technologies) and the eluted protein was dialyzed against PBS and used as immunogen for antiserum generation.
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (HI-FCS, Gibco) and 1% of Penstrep (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) and 1% penicillin-streptomycin (Thermofisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Initialization was performed using the IonTorrent Hi-Q View Kit (Thermofisher) with Ion 318 Chip Kit v2 BC (Thermofisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Biophysics 2020Quote: ... The cRNA was transcribed using the T7 mMessage mMachine kits (ThermoFisher Scientific). Defolliculated Xenopus laevis oocytes were purchased from Ecocyte Bioscience (Austin ...
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Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotencybioRxiv - Developmental Biology 2021Quote: ... the plasmid underwent in vitro transcription using mMESSEAGE mMACHINE T7 kit (Invitrogen) and were purified by LiCl precipitation ...
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bioRxiv - Cell Biology 2020Quote: gRNAs were generated from gBLOCKS with MEGAshortscript™ T7 Kit (Invitrogen, AM1354). Following DNaseI treatment and precipitation with NH4Ac and ethanol ...
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bioRxiv - Genetics 2021Quote: ... capped RNAs were transcribed with a T7 mMessage mMachine kit (AM1344, Thermofisher), and embryos from natural spawning were injected with up to 600pg RNA in phenol red (P0290 ...
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bioRxiv - Genomics 2021Quote: ... we used ‘mMESSAGE mMACHINE T7 ULTRA Transcription kit’ (Invitrogen, Cat. No. AM1345) at 37 °C for 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... sgRNA were obtained by in vitro transcription with MEGAshortscript T7 kit (Ambion). CAS9 mRNA was obtained by in vitro transcription of linearised pT3TS-nCas9n vector (Addgene #46757 ...
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bioRxiv - Developmental Biology 2020Quote: ... Capped mRNA was synthesized using the mMessage T7 kit (Ambion, Austin, TX) according to the manufacturer’s instructions and purified by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... gRNAs were in vitro transcribed using the MEGAshortscript T7 kit (Life Technologies) and purified using 3M sodium acetate/ethanol precipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... synthesis the annealed oligonucleotides was cloning into the sgRNA expression vector pDR274 (Addgene #42250)[38] and synthesized using the MEGAshortscript T7 Transcription Kit (Thermo Fisher Scientific). Additionally ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were synthesized overnight with the MEGAscript T7 Transcription Kit (Invitrogen; AM1334). Afterwards sgRNAs were cleaned-up with the mirVana miRNA Isolation Kit (Ambion ...
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bioRxiv - Biochemistry 2022Quote: ... mRNA was made with the mMESSAGE mMACHINE T7 Ultra Kit (Life Technologies) from linearized DNA encoding the human α2 with mutations to decrease ouabain sensitivity (Q116R and N127D ...
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bioRxiv - Molecular Biology 2021Quote: ... Germany) were synthesized using the T7 mMessage mMachine® kit (Ambion, USA). The SmHDAC8-pGBKT7 ...
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bioRxiv - Molecular Biology 2020Quote: ... using the mMESSAGE mMACHINE® T7 Ultra Kit (Life Technologies, Carlsbad, CA) and purified by Lithium Chloride precipitation ...
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bioRxiv - Genomics 2020Quote: We synthesized capped CLH-1 cRNAs using T7 mMESSAGE mMACHINE kit (Ambion) and purified by lithium chloride precipitation ...
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bioRxiv - Developmental Biology 2019Quote: ... Firefly luciferase and AcGFP sequences using the MEGAscript T7 kit (Thermo Fisher) with 0.1 μM PCR product as the template ...
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bioRxiv - Molecular Biology 2019Quote: ... the transcription reactions were performed using T7 in vitro transcription system (Ambion) in presence of [32P] UTP and riboprobes obtained were used in binding experiments with M14 and B-LCL#5 S100 extract ...
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bioRxiv - Immunology 2019Quote: ... Casp1 and Casp11 retrotranscription we used mMessage mMachine T7 Ultra (ThermoFisher Scientific) following kit instructions ...
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bioRxiv - Genomics 2019Quote: ... sgRNAs were transcribed using the MEGAshortScript T7 Transcription Kit (Thermo Fisher Scientific) with overnight incubation and purified using the MEGAclear Transcription Clean-Up Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... The dsRNAs were synthesized using MEGAscript T7 Transcription Kit (Ambion, Texas, USA).
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bioRxiv - Genomics 2020Quote: ... purified with phenol/chloroform and transcribed with T7 RNA polymerase (ThermoFisher Scientific). Final sgRNAs were purified as described previously36 ...
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bioRxiv - Cell Biology 2020Quote: mRNAs were synthesized using an mMESSAGE mMACHINE T7 kit (Thermo Fisher Scientific) and were purified at a final concentration of 1.0 to 1.5 μg/μL ...
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bioRxiv - Cell Biology 2020Quote: ... the pTRI-RNA 18S control construct (MEGAshortscript™ T7 Transcription Kit, Ambion) was used as a DNA template for the in vitro transcription ...
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bioRxiv - Bioengineering 2022Quote: IVT reactions were performed using the Ambion MEGAscript T7 IVT kit (ThermoFisher). For unmodified ss/dsRNA ...
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bioRxiv - Microbiology 2022Quote: ... and in vitro transcription was performed using the MEGAscript T7 kit (Invitrogen) and incubation at 37°C for 12 hours ...
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bioRxiv - Genetics 2022Quote: The dsRNA targeting Dmai-BicC (dsRNABicC) was synthesized using T7 Polymerase (ThermoFisher), according to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2022Quote: ... followed by in vitro transcription using MEGAscript T7 Transcription Kit (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The cRNAs were synthesized with mMESSAGE mMACHINE T7 kit (Thermo Fisher Scientific) from the linearized cDNAs ...
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bioRxiv - Developmental Biology 2022Quote: ... Antisense RNA probe was synthesized using MEGAscript T7 Transcription Kit (Ambion, USA) with DIG RNA Labeling Mix (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Transcription of sgRNA was carried out using MEGAshortscript T7 kit (Ambion/Invitrogen) with 100-400ng of purified DNA following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Transcription was performed using a MEGAscript T7 Transcription kit (Thermo Fisher Scientific) following the manufacturer’s instructions and purified by exclusion chromatography on a TSKgel G4000SW column as previously described (40) ...
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bioRxiv - Molecular Biology 2020Quote: ... This was converted to RNA using the MEGAshortscript T7 kit (Fisher Scientific). sgRNA (50ng/ml each ...
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bioRxiv - Neuroscience 2021Quote: ... In vitro transcription was performed using the MAXIscript T7 kit (Life Technologies) and RNA was purified using the MEGAclear kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg of dsRNA generated using the T7 Transcription Kit (ThermoFisher AM1334) was incubated with cells for 45 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Transcription of sgRNA was carried out using MEGAshortscript T7 kit (Ambion/Invitrogen) with 100-400ng of purified DNA following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Capped mRNAs were synthesized using a T7 polymerase (mMessage mMachine kit, Ambion). mRNA concentrations were measured using a Nanodrop spectrophotometer (Thermo Scientific).
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bioRxiv - Genetics 2019Quote: DsRNA was generated using the MEGAscript™ T7 Transcription kit (ThermoFisher #AM1334), with primers for EGFP (F ...
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bioRxiv - Evolutionary Biology 2019Quote: ... then double-stranded RNA was synthesized using a T7 MEGAscript Kit (Ambion). Parental RNAi was performed by injecting 3-day-old pupae which were randomly chosen from the wild type stock ...
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bioRxiv - Bioengineering 2019Quote: ... MEGAshortscript T7 kit and mirVana miRNA isolation kit were purchased from Invitrogen Australia ...