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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for 7 BROMO 2 3 4 5 TETRAHYDRO 1H BENZO E 1 4 DIAZEPINE 2 CARBOXYLIC ACID METHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... 20 μmol EdU (5-ethynyl-2-deoxyuridine, Life Technologies) was added 30 min prior washing with PBS and fixation with 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2022Quote: ... EdU (5-ethynyl-2’-deoxyuridine, ThermoFisher Scientific, cat# A10044) staining was performed by Click-it Alexa Fluor 647 imaging kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and 5 ng/ml IL-2 (Thermo Fisher Scientific), and then resuspended with 1 ml of virus per 6 x 106 cells ...
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bioRxiv - Cell Biology 2021Quote: 0.1mg of 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen, A10044) dissolved in PBS (50ul ...
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bioRxiv - Developmental Biology 2020Quote: 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen A10044, Carlsbad, CA) dissolved in PBS was administered to mice at indicated postnatal days ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μM 5-ethynyl-2’-dexoyuridine (EdU, ThermoFisher Scientific) was added to the culture media for 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: 5-ethynyl-2′-deoxyuridine (EdU) was purchased from ThermoFisher and stock solutions were created by dissolving the powder in dimethyl sulfoxide as instructed by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU, Molecular Probes, Life Technologies) was dissolved in DMSO to make a 10mM stock solution ...
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bioRxiv - Cancer Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU, Molecular Probes, Life Technologies) was dissolved in DMSO to make a 10mM stock solution ...
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bioRxiv - Genomics 2022Quote: EdU (5-ethynyl-2’-deoxyuridine) (Thermo Fisher Scientific, A10044) was added to culture media at 10 µM final concentration for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μl of 5 × Maxima RTA buffer (Thermo Scientific), which were mixed with Ultrapure water (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% FCS and 2% B-27 (Gibco) and maintained at 5% CO2 and 37°C in humidified incubators ...
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bioRxiv - Neuroscience 2023Quote: EdU (5-ethynyl-2’-deoxyuridine; #A10044, Thermo Fisher Scientific) was diluted at 10mg/ml in 0.9% NaCl (#114-055-101 ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μg plasmid and 5 μl Lipofectamine 2000 (Invitrogen) were diluted into 200 μl Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... CellEvent Caspase-3/7 Detection Reagent (Invitrogen) and Hoescht were added to the media at concentrations listed in S4 Table and incubated for 30min at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... CellEvent Caspase-3/7 (Life Technologies C10723), Fis1 polyclonal antibody (Proteintech 109561-AP) ...
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bioRxiv - Cell Biology 2023Quote: ... Caspase 3/7 (Thermo Fisher Scientific, #C10423) activation and propidium iodide (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and caspase 3/7 (Thermo Fisher Scientific) were prepared following the manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: Fibroblasts were plated in 96-well plates (10,000 fibroblasts/well) for 3 days in 2% FBS in DMEM supplemented with ascorbic acid (50 μg/mL) (Fisher Scientific, Waltham, MA, USA). Fibroblasts and matrix were then fixed and immunostained as detailed above ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μM Rhod-2 AM (Thermo, R1244) and 2 μM Calcium green-1 AM (Life Technologies, C3011MP) was added to culture media and allowed to stain at 22 °C for 20 min prior to imaging (frozen Rhod-2 AM aliquots were used only when the DMSO suspension remained clear) ...
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bioRxiv - Evolutionary Biology 2020Quote: ISH and dFISH were performed as previously described (3, 7, 62) with addition of T1 RNAse treatment (2 u/µl, Thermo Fisher Scientific, Waltham, MA, USA) after probe washing in embryos older than 4 dpf to reduce background staining ...
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bioRxiv - Bioengineering 2021Quote: Human lung fibroblasts (hTERT T1015, abmgood) were used between passages 7-12 and culture medium was changed every 2-3 days (Gibco Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescently tagged G-actin was prepared by covalent modification with Alexa Fluor™ 594 Carboxylic Acid (Thermo Fisher Scientific 15461054) (Alvarado and Koenderink ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 2 weeks of selection with 3 μg mL−1 of puromycin (Thermo Fisher Scientific). Then ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight 4°C incubation with rabbit polyclonal antibodies against claudin-5 (1:100; Life Technologies) and ZO-1 (1:200 ...
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bioRxiv - Genomics 2022Quote: ... 4 μl of 1 M CaCl2 and 5 μl of 100 U MNase (88216, Thermo Fisher Scientific) were added ...
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bioRxiv - Molecular Biology 2019Quote: ... 3-9) or 5 µl (IP: Figure 2A-2C and input: Figure 2-9) RNAse A/T1 (Thermo Scientific #EN0551) and incubation the beads at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Immunology 2019Quote: ... isolated lungs were cut into 2-3 mm2 sections and resuspended in 5 mL digestion solution of Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco) supplemented with 5 mM GlutaMax (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were separated using 50 cm Acclaim PepMap 100 analytical column (75 μm ID, 3 μm C18) in conjunction with a Pepmap trapping column (100μm × 2 cm, 5 μm C18) (Thermo Scientific) analysed with Orbitrap Fusion Tribrid mass spectrometer (Thermo-Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... For the construction of the RNH2A KO clones, RNASEH2A (Chr19, exon 2) gRNA (5’-TAACAGATGGCGTAGACCAT-3’) was cloned into GeneArtTM CRISPR Nuclease Vector with OFP reporter (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
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bioRxiv - Cell Biology 2024Quote: ... 12 alternating fractions were resuspended in 2% formic acid/5% acetonitrile and subsequently injected for analysis on an Orbitrap Eclipse Tribrid (Thermo Fisher Scientific). For the yeast cell PISA ...
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bioRxiv - Microbiology 2023Quote: ... niveus CO7 DNA ranging from 7 to 7 × 10−4 ng/µl was generated and quantified using a Nanodrop-8000 Spectrophotometer (Thermo Scientific, Wilmington DE, USA) (260/280 ratio of 1.89) ...
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bioRxiv - Biochemistry 2021Quote: The KDELR/Legobody complex at 0.8mg/ml was PEGylated by incubation with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher) at a 1:40 molar ratio for 2 hrs on ice to reduce preferred particle orientation on the grids ...
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bioRxiv - Biochemistry 2022Quote: Changes in the mitochondrial membrane potential were determined as the changes in tetramethylrhodamine methyl ester (TMRM, T668, Thermo Fisher Scientific) fluorescence according to the manufacturer’s instructions with few modifications ...
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bioRxiv - Microbiology 2023Quote: ... aeruginosa growth support by pyochelin and pyochelin methyl ester were performed in M9 minimal medium supplemented with 500 µM 2,2’-Bipyridine (Fisher Scientific). M9 minimal medium was prepared as follows ...
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bioRxiv - Immunology 2023Quote: ... cDNA was diluted 1:3 or 1:4 before mixing with Power SYBR Green PCR Master Mix (CAS: 4368702, Applied Biosystems) and primer pairs (Table S4B) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged at 14,000g for 5 minutes at 4°C and electrophoresed on NuPAGE™ 4-12% Bis-Tris Polyacrylamide gels (Thermo Fisher) with NuPAGE™ MOPS running buffer (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... (4) 30 min incubation in ammonium chloride (NH4Cl) and (5) 4 min incubation in Tissue Autofluorescence Quenching Kit (ReadyProbes, ThermoFisher Scientific).Secondary antibodies were used as follows ...
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bioRxiv - Immunology 2021Quote: ... domain of the S-protein were labeled with 4 different fluorophores (Alexa Fluor 647, 488, 594 and 405) using Alexa Fluor NHS Ester kit (Thermo Scientific), were mixed and incubated with S-protein-beads ...
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bioRxiv - Biophysics 2022Quote: ... the antibody produced in-house from HPC-4 hybridoma, ATCC HB-9892, and labeled with Alexa Fluor 647 NHS Ester, Thermo Fisher), showing superior Wnt display on the hFzd5CRD-1TM cells ...
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bioRxiv - Cancer Biology 2022Quote: ... The samples were loaded in loading buffer (2% MeCN, 0.05% trifluoroacetic acid) and bound to an Acclaim Pepmap 100 µm × 2 cm trap (Thermo Fisher Scientific), and washed for 10 min to waste ...
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bioRxiv - Immunology 2024Quote: ... Digestion was stopped by adding acetonitrile to 2% and trifluoroacetic acid to 0.3% and the beads were collected with DynaMag-2 Magnet (Thermo Fisher Scientific). Digested peptides were purified by loading the supernatant into C18 StageTips (58) ...
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bioRxiv - Microbiology 2021Quote: ... We mixed an equal volume of the extracted LPS samples with an equal volume of 2× SDS buffer and electrophoresed 10 µl of each sample on Novex 4-20% plyacrylamide gradient gels (ThermoFisher) in Tris-Glycine-SDS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 0.2% (v/v) Triton X-100) and analyzed by SDS-PAGE on 4-12% NuPAGE Bis-Tris gradient gels (Invitrogen) followed by immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed twice with 1X PBS and stained with either DAPI (4’,6-diamidino-2-phenylindole, from Life Technologies), for the visualization of the nucleus ...
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bioRxiv - Developmental Biology 2021Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...