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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: 1 µg of total RNA was reverse transcribed in a 20 µL final reaction volume using the High Capacity cDNA Reverse Transcription Kit (Life Technologies) with RNase inhibitor and random primers following the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... The RNA was then purified and concentrated to 20 µL with the GeneJet RNA purification Kit (Thermo Fisher Scientific, Ecublens, Switzerland). For each series of RNA extraction (12 samples) ...
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bioRxiv - Immunology 2020Quote: ... the cells were rinsed with PBS and labelled with calcein-AM for 20 minutes at 37°C from Live/Dead Viability/Cytotoxicity kit for mammalian cells (Thermo Scientific). After staining ...
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bioRxiv - Genomics 2019Quote: ... Completed reactions were run on 5% or 4-20% native acrylamide gel and transferred blots were developed using LightShift Chemiluminescent EMSA kit (Thermo Scientific) and imaged on Chemidoc Touch (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... 20 ng of RNA isolated from sorted cells was retro-transcribed to cDNA using SuperScript VILO cDNA Synthesis Kit (ThermoFisher Scientific). Barcoded libraries were prepared using the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit as per the manufacturer’s protocol and sequenced using an Ion S5 system (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng of total RNA was converted into cDNA in a 20 μl reaction using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Life technologies ...
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bioRxiv - Plant Biology 2019Quote: ... Synthesis of cDNA was carried out with 500 ng of total RNA added to each 20 µl reaction of the High Capacity cDNA Reverse Transcription Kit (Thermo Scientific). The cDNA samples were stored at −20°C for downstream analysis ...
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bioRxiv - Cell Biology 2021Quote: ... total RNA from testicular explants (n = 20 males) was extracted by using the commercial RNAqueous®-Micro kit (Ambion, Austin, USA), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Membranes was washed again with TBST for 20 min x 3 times and revealed using the Super Signal West Pico kit (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from 20 μl of serum or from homogenized tissues in TRIzol reagent using the PureLink RNA mini kit (Life Technologies). CHIKV cDNA was generated from 10 μl of serum derived RNA or 1 μg of tissue derived RNA using random primers (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... 500 ng template RNA was reverse transcribed with random hexamer primers in 20 μL using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher) and subsequently diluted 1:3.3 with nuclease-free water ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was prepared from 25 ng of RNA using oligoDT primers in a 20 µl reaction using a High Capacity cDNA Reverse Transcription Kit (ThermoFisher, 4368814). qRT-PCR reactions were performed in real time using 5 µl PowerUp SYBR Green Master Mix (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... The 80 μL PCR product was concentrated down to 20 μL using the GeneJET PCR Purification kit (Thermo Fisher cat# K0702). PCR products were eluted into 20 μL of water ...
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bioRxiv - Plant Biology 2022Quote: ... Synthesis of cDNA was performed with 700 ng of RNA in a total reaction volume of 20 μl using the Superscript III kit (Invitrogen, Germany). For qRT-PCR analysis ...
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bioRxiv - Plant Biology 2022Quote: ... A total of 200 ng of DNA-free RNA samples was reverse transcribed in a final volume of 20 µL using the RNA to cDNA kit (Life technologies). cDNAs were then diluted ten times ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized from reverse transcription on 1.0 μg total RNA in a 20 μL reaction using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, U.S.A.) and T100 thermal cycler (BIO-RAD ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was eluted with 20 μl of RNase-free water and yield was determined fluometrically using Qubit RNA high-sensitivity assay kit (Thermo Fisher).
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bioRxiv - Biophysics 2023Quote: ... The amount of Pi released from actin was measured by absorbance at 360 nm every 20 seconds for 30 minutes to 1 hour using an EnzChek phosphate assay kit (Thermo Fisher) (63 ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA (1 μg) was reverse-transcribed in a 20 μL reaction using RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, #K1622). After reverse transcription at 42°C for 60 min ...
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bioRxiv - Cell Biology 2023Quote: ... samples were collected by centrifugation (11,000 ×g, 20 min) and their protein content was determined (Thermo Pierce BCA Protein Assay kit, ThermoFisher, Cat# 23225). Proteins (∼30 µg/lane ...
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bioRxiv - Cancer Biology 2023Quote: ... RT-PCR was carried out using 500 ng of total RNA in a 20 µL volume using SuperScript IV One-Step RT-PCR kit according to the manufacturer’s protocol (ThermoFisher, Cat# 12594025) with a Taqman probe specific to POSTN (Hs01566750 ...
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bioRxiv - Neuroscience 2023Quote: ... Reverse transcription was performed in similar amounts of RNA to produce cDNA in a 20 μl reaction using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... 250 ng of RNA was converted to complementary DNA (cDNA) in a 20 ul reaction using the Applied Biosystems High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 20 clones from each plate were expanded and genomic DNA extracted by a GeneJet Genomic DNA Purification Kit (ThermoFisher Scientific, K0722) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA was prepared by loading 2 µg of RNA per sample into a 20 µL reverse transcription (RT) reaction using a High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a BCA protein assay kit (Pierce) and 15-20 μg of protein was separated on a 4-12% NuPAGE Bis-Tris gel (Life Technologies) followed by transfer to PVDF membranes ...
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bioRxiv - Genomics 2024Quote: ... Reverse transcription was carried out on 0.1-0.5 μg total RNA per sample in a 10 or 20 μl reaction using the SuperScript VILO Reverse Transcription Kit (Invitrogen, 11754). 10 ng of cDNA was used per PCR reaction with Perfecta SYBR Green Fastmix (Quanta BioSciences ...
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bioRxiv - Cell Biology 2024Quote: Cells were transfected with control and gene-specific siRNAs at a final concentration of 20 nM using the Neon transfection system 100 µl kit (ThermoFisher, MPK100) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and 20 ng were used for reverse transcription using the RevertAid H Minus First Strand cDNA Synthesis kit (ThermoFisher Scientific, USA) according to manufacturer’s recommendations ...
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bioRxiv - Biophysics 2019Quote: ... proteasome samples (1 μM) were buffer exchanged (Zeba Micro Spin Desalting Columns, Thermo Fisher Scientific) into 25 mM BisTris ...
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bioRxiv - Plant Biology 2020Quote: ... using oligo(dT)20 and RevertAid Reverse Transcriptase (Thermo Fisher Scientific™) in a 20 μL-reaction volume ...
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bioRxiv - Plant Biology 2020Quote: ... 645 and 663 nm using the spectrophotometry (Thermo Scientific Gensys 20, USA). Chlorophyll (a and b and total ...
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bioRxiv - Biochemistry 2020Quote: ... in PBS + 0.1% Tween 20 (Fisher Scientific, Fair Lawn NJ, BP337-500). Probing was performed for 2 hours with the following primary antibodies at 1:1000 in blocking buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... with cells being transfected at 20 –30% confluence using Lipofectamine RNAiMAX (Invitrogen) as described[21-23] for 48 hr before analysis of target knockdown and effects on HPV16 transcription.
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bioRxiv - Cancer Biology 2021Quote: ... for 20 min and Hoechst 33342 (Cat No: H1399, Life Technologies™.) for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... and chromatin was digested with 20 units of MNase (Thermo Fisher, EN0181) at room temperature for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... and pulse-labelled with 20 µM 5-ethynyl deoxy-uridine (EdU; Invitrogen) and 1 µM FuDR (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 μL of 20 μg/μl glycogen (Invitrogen™ UltraPure™ Glycogen) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 7.4] supplemented with 0.05% Tween-20 (TBS-T) (Thermo Fisher Scientific) or in 5% bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... fifteen uL of TMT reagent (20 mg/mL, ThermoFisher cat# 90111 & A34807) were added per each 100 ug of protein ...
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bioRxiv - Developmental Biology 2021Quote: ... cell lysates were loaded on a 4 - 20% Bis-Tris gel (Invitrogen) and proteins blotted onto a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Genomics 2020Quote: ... Sheared chromatin was precleared with 20 μl Dynabeads Protein A (Life Technologies) at 4 °C for 1 hr.
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bioRxiv - Plant Biology 2022Quote: ... the linearized plasmid was digested with 20 U DNase I (Thermo Scientific) for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... for 20 min at room temperature and then washed in PBS (Invitrogen). Cellular membranes were labeled with 5 μg/ml wheat germ agglutinin (WGA ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was digested in 20 µg/mL Proteinase-K (Thermo Fisher AM2548) in 0.1% PBS-Tween for 30 min at room temperature and washed twice with 0.1% PBS-Tween-20 for 10 min each at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µm thick sections were cut using a NX70 Cryostat (ThermoFisher Scientific) and thaw-mounted onto Superfrost Plus™ microscope slides (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were selected with 20 µg/ml Bla (Thermo Fisher Scientific, A1113903) for five days ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were pre-incubated with 20 μM of EdU (Life Technologies; A10044) for 30 min after irradiation and 30 min prior to fixation and processed as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... with 5–20% fetal bovine serum (Thermo Fisher Scientific Cat#12483–020). After five to seven days ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µl reaction volume was loaded onto 6% DNA retardation gels (Invitrogen), and electrophoresis was carried out at room temperature in 0.5x Tris borate buffer ...