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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 6 Hydroxy 1H indole 3 carboxylic acid methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 6d ovaries underwent 1h of EdU labelling based on the protocol of the Click-iT™ Plus EdU Cell Proliferation Kit for Imaging (Invitrogen). Both 6d and 12d ovaries were stained for Fasciclin III (Fas3 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat polyclonal antibodies conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/100 in FACS buffer ...
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bioRxiv - Physiology 2023Quote: ... Freshly dissected SCG were digested 1h at 37°C in trypsin 1X (Trypsin 10X, Life technologies, diluted 1:10 in DPBS). Sympathetic neurons were dissociated ...
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bioRxiv - Neuroscience 2022Quote: ... Protein lysates were pre-cleared 1h at 4°C on a rotating wheel with 50μl of previously washed Dynabeads Protein G (Invitrogen, Thermo Fisher Scientific), to avoid aspecific bindings ...
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bioRxiv - Cell Biology 2022Quote: ... donkey anti-sheep antibodies were incubated for 1h and after thorough washing and blocking with 5% normal goat serum (Life Technologies) for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 in blocking buffer for 1h and counterstained with DAPI prior to mounting with ProLong Gold anti-fade reagent (Invitrogen, P36930) for subsequent immunofluorescence analysis.
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 1h at RT with Alexa fluor dye-conjugated secondary antibodies (1:500) and Alexa-555-coupled phalloidin (1:400, Invitrogen, A34055) diluted in PBS buffer supplemented with 0.5% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... Novusbio # NBP1-78278) was incubated for 1h at room temperature and revealed with a Pacific Orange-conjugated secondary antibody (1:50, ThermoFisher, # P31584). After DAPI counterstaining ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with peroxidase-linked secondary antibodies for 1h at RT diluted in blocking buffer and then treated with Supersignal West Pico solution (Thermo Scientific). Images were acquired on an LAS-3000 system (Fuji Film).
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bioRxiv - Microbiology 2023Quote: ... diluted at 1:250 in blocking solution was incubated 1h at room temperature followed by a 45min incubation of a goat anti-rabbit Alexa-488 secondary antibody (Invitrogen, #A11034) diluted to 1:500 together with Hoechst and rhodamine-phalloidin ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed and then incubated with the corresponding secondary antibodies for 1h at room temperature (Goat Anti-rabbit Secondary Antibody, HRP conjugate 1:1000, ThermoFisher #31460). All antibodies were diluted in a 5% BSA solution in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: Lumbar spinal cord and hippocampal sections were rinsed in 0.1M PB and subsequently incubated at room temperature for 1h with 30% (v/v) normal goat serum (Invitrogen, Cat#31872) in 0.1M PB containing 10% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... with 63x immersion objective magnification after 1h incubation with secondary antibodies (goat anti-mouse IgG 546 (Molecular Probes, Cat. no. A11030) and goat anti-rabbit IgG 488 (Molecular Probes ...
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bioRxiv - Neuroscience 2023Quote: ... the cells incubated with secondary antibodies in blocking solution for 1h at RT in the dark (anti-chicken Alexa 568, 1:1000, A11041, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... After washing the coverslips with PBS, samples were incubated with appropriate secondary antibodies (1:1000, 1h at room temperature) from Thermo Scientific: goat α-rabbit combined with Alexa-405 (#A-31556) ...
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bioRxiv - Genetics 2024Quote: ... samples were washed three times with PBS and incubated for 1h with the secondary antibodies Alexa Fluor 647-conjugated chicken anti-rabbit (1:200, #A21443, Life Technologies), Alexa Fluor 488-conjugated goat anti-mouse (IgG2a ...
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bioRxiv - Developmental Biology 2024Quote: ... Brains were washed again in PBT for 1h prior to incubation with anti-ZO-1 antibody (ZO1-1A12, Thermofisher, 1:150) in staining solution (2% normal goat serum ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated 1h at RT in the dark with a secondary antibody solution containing goat anti-mouse Alexa 594 (A11032, Invitrogen, Fisher Scientific) fluorescent-coupled antibody diluted to 1:1000 in PBS-T solution ...
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bioRxiv - Bioengineering 2021Quote: ... or a long-chain fatty acid (Hexadecanoic Acid, C16; BODIPY™ FL C16, D3821, Thermo Fisher Scientific Inc.) were added at a concentration of 4 µM to the culture medium as defined by culture mode (table 3) ...
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bioRxiv - Microbiology 2020Quote: ... total nucleic acid was extracted from tissues using the MELT™ Total Nucleic Acid Isolation System (Ambion, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... We then proceeded with a standard hot phenol-acid extraction (Acid Phenol:Chloroform mix 125:24:1, Invitrogen, AM9722). The samples were resuspended in nuclease-free water and quantified on a Spectrophotometer ...
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bioRxiv - Neuroscience 2021Quote: ... 0.8 mM KH2PO4, 20 mM NaHCO3, 1.3 mM L-glutamine, 0.2 mM ascorbic acid, MEM amino acids solution [Gibco; Thermo Fisher ...
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bioRxiv - Pathology 2023Quote: ... Dilutions of SCFAs standards (Acetic acid:A6283, Sigma-Aldrich; Propionic acid: 81,910, Sigma-Aldrich; Butyric acid:108,111,000, Acros Organics) were prepared in saline solution at concentrations ranging from 87 to 0.087 mM for acetic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... omitting all amino acids without the addition of non-essential amino acids and substitution with dialyzed FBS (Invitrogen). Doxycycline treatment of cells stably expressing ER-phagy probe was performed as previously described7.
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bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... 3 (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
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bioRxiv - Genomics 2022Quote: ... Bisulfite-PCR was conducted using primer sets (Integrated DNA Technologies; Table 1) designed against specific genomic regions with Methyl Primer Express v1.0 software (Thermofisher Scientific). PCR amplicons were cleaned with AmpureXP beads (#A63882 ...
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bioRxiv - Biochemistry 2020Quote: ... N,N-dimethyl formamide (DMF) and water and HPLC grade methyl tert-butyl ether (MTBE) were obtained from Thermo Fisher used throughout ...
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bioRxiv - Neuroscience 2019Quote: ... were designed based on the appropriate National Center for Biotechnology Information (NCBI) reference genome using the Methyl Primer Express v1.0 software (Thermofisher Scientific) to amplify 250-350 bp regions of interest upstream or downstream the transcription start site (TSS ...
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bioRxiv - Systems Biology 2021Quote: ... A total of 90 μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (Thermo Scientific, Lafayette, CO, U.S.A.) was then added to the samples and shaken at 1400 rpm for 30 min at 37 °C ...
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bioRxiv - Microbiology 2019Quote: QSG-MSP was performed to quantify the levels of CpG DNA methylation of CD4 and RUNX3.Primers for QSG-MSP were designed using Methyl Primer Express Software v1.0 (Applied Biosystems). Primer sequences for CD4 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured for 24 hours in culture medium supplemented with 0.5% (w/v) methyl cellulose and B-27 (1/50, Life technologies).
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bioRxiv - Neuroscience 2021Quote: ... while levels of IL-6 in supernatants were determined using IL-6 mouse ELISA kit (Thermo Scientific). Similarly ...
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bioRxiv - Immunology 2021Quote: ... 5/6 weeks and 6/7 weeks post-infection by flow cytometry using counting beads (CountBright, ThermoFisher).
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bioRxiv - Microbiology 2022Quote: ... transferred to 6 ml liquid MSgg medium of each well of a 6-well microplate (Thermo Scientific), and then grown for additional four days ...
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bioRxiv - Microbiology 2020Quote: ... 6 × 105 293LTV cells were seeded in a 6-well plate and transfected using Lipofectamine 2000 (Invitrogen) which was complexed with DNA plasmids driving the expression of either VSV G protein (positive control) ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... Coding regions were transferred into Pvha-6-GFP or Pvha-6-RFP vectors by LR reaction (Invitrogen). Constructs were bombarded into unc-119(ed3 ...
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bioRxiv - Cancer Biology 2023Quote: ... ANBL-6 cells were also supplemented with 5 ng/ml IL-6 (Thermo Fisher Scientific, Cat# 206IL010).
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bioRxiv - Biophysics 2023Quote: 6-well plate cell proliferation – Cells were seeded into 6-well plates (Fisher Scientific, 07-200-83) 150,000 cells/well and left at ambient temperature for 20 minutes to ensure homogeneous settling ...
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Controlled Fluorescent Labelling of Metal Oxide Nanoparticles for Artefact-free Live Cell MicroscopybioRxiv - Biophysics 2021Quote: ... 1% NEAA (non-essential amino acids, Gibco) in an incubator at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Acid-extracted rat tail collagen (Gibco, A1048301) was neutralized on ice in the presence of 10X DMEM with 0.1N NaOH such that the final concentration of the collagen is 1 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... and 1x non-essential amino acids (Gibco), under 5% CO2 at 37°C in a humidified atmosphere ...
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bioRxiv - Cancer Biology 2021Quote: ... 1X non-essential amino acids (Gibco, 11140035), 100μg/mL penicillin-streptomycin (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 mM MEM nonessential amino acids (Gibco) and 1000 U/ml LIF (Millipore) ...