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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 6 Hydroxy 1H indole 3 carboxylic acid methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: In vivo Δψm was measured using the cell-permeant red-fluorescent dye TMRE (tetramethylrhodamine ethyl ester, Thermo Fisher Scientific). For each time point ...
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bioRxiv - Bioengineering 2020Quote: ... the cell mixtures were stained with 2.5 µM carboxyfluorescein diacetate succinimidyl ester (CFDA SE, ThermoFisher, referred to as CFSE) before restimulation with anti-CD3/CD28 beads ...
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bioRxiv - Biochemistry 2020Quote: ... NosRcaΔC and NosRca-IFmut was labelled at the N terminus with the fluorophore Alexa Fluor 405 NHS ester (ThermoFisher) (∼2.2 ...
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bioRxiv - Biophysics 2021Quote: ... as specified by the commercial protocol) and a fluorescent tag (Alexa Fluor® succinimidyl esters, Invitrogen™, Molecular Probes® ...
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bioRxiv - Biophysics 2020Quote: A1-LCD was fluorescently labeled on the N-terminus using Alexa Fluor® 488 NHS ester fluorescent dye (ThermoFisher) to label the primary amines of proteins ...
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bioRxiv - Cancer Biology 2022Quote: Proliferation was measured by labelling primary CD8+ T-cells with 1 μM carboxyfluorescein diacetate succinimidyl ester (CFSE, Thermo Fisher) for 10 min at 37°C before activation and treatments ...
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bioRxiv - Immunology 2022Quote: ... The barcoding dyes which were used in this study were Pacific Orange and Alexa Fluor 700 NHS esters (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Purified vesicles were covalently labelled with an amine-reactive Alexa Fluor 488 5-SDP ester dye (Molecular Probes/ThermoScientific).
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Immunology 2022Quote: Fn purified from human plasma (sigma) or monoclonal antibodies were fluorescently conjugated using AlexaFluor 488 and 647 Ester (Invitrogen). The conjugation and purification were done according to the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2019Quote: ... After rinsing with a solution containing NPE-caged ATP (adenosine 5′-triphosphate, P3-(1-(2-nitrophenyl) ethyl) ester) (Invitrogen) (0 to 1 µM ...
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CRISPR-Csx28 forms a Cas13b-activated membrane pore required for robust CRISPR-Cas adaptive immunitybioRxiv - Biochemistry 2021Quote: Protein in vivo crosslinking was carried out with NHS ester, DSS (disuccinimidyl suberate, spacer arm 11.4 Å) (Thermo Scientific) also as suggested by the manufacturer ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... non-viable Nb were incubated in 0.05 M NaHCO3 buffer and 0.1 mg AF488 NHS Ester (Molecular Probes, Invitrogen) for 15 minutes and washed with 0.1M Tris buffer according to the manufacturer’s instructions.
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... non-viable Nb were incubated in 0.05 M NaHCO3 buffer and 0.1 mg AF488 NHS Ester (Molecular Probes, Invitrogen) for 15 minutes and washed with 0.1M Tris buffer according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Virions were then labeled with 100 µg of Alexa Fluor™ 488 TFP ester following the manufacturer’s instructions (ThermoFisher). Unincorporated dyes were separated from labeled virions using PD-10 gel filtration columns ...
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bioRxiv - Neuroscience 2022Quote: ... by incubation for 40 min in Krebs solution containing 20 μM calcein AM (acetoxymethyl ester of calcein; ThermoFisher Scientific). The slices were then washed three times in normal Krebs solution and fixed for at least 2 hours at RT or overnight at 4 °C in PBS PAF 4% ...
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bioRxiv - Microbiology 2022Quote: ... Cleared lysate from a T75 flask was labelled with 50 μg Alexa Fluor 647 succinimidyl ester (ThermoFisher Ref A20006) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Bovine fibrinogen is labeled with a fluorescent reactive dye (Alexa Fluor 647 NHS succinimidyl ester, Thermo Fisher Scientific, Waltham). After purification from the unreacted dye via dialysis ...
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bioRxiv - Cell Biology 2023Quote: ... We washed the beads twice with phosphate-buffered saline (PBS) and incubated them with 0.5 mL PBS containing 0.05 µg Rhodamine Red-X succinimidyl ester (ThermoFisher Scientific) for 5 min ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were stained with carboxifluorescein diacetate succinimidyl ester (CFSE) using the CellTrace CFSE Cell Proliferation Kit (Invitrogen, C34554) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Ca2+ imaging of mouse islets was performed with Fura-2 AM (Fura-2-acetoxymethyl ester) acquired from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biophysics 2023Quote: BRICHOS was labeled with Alexa-488 by mixing 43.6 µM BRICHOS dissolved in 100 mM bicarbonate buffer (pH 8.3) and Alexa-488 NHS Ester (Thermo Scientific) dissolved in DMSO in a 1:2 molar ratio followed by 1 h incubation at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were treated with CM for 48 hours before loading with 2 μM fura-2 acetoxymethyl ester (Molecular Probes) in calcium imaging buffer (CIB ...
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bioRxiv - Cell Biology 2020Quote: ... EndoB1 siRNA 5’-UGUUUAUACGACUUGGAGCUU-3’ and 3’-AAGCUCCAAGUCGUAUAAACA-5’ (Invitrogen), control siRNA (Ambion) ...
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bioRxiv - Microbiology 2021Quote: ... FluoZin-3 (FluoZin™-3, AM, cell permeant, Thermo Fisher) was added at 1 mM ...
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bioRxiv - Immunology 2020Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific). The activated beads were washed three times with 50 mM MES pH 5.0 and added to SARS-CoV-2 S protein which was diluted in 50 mM MES pH 5.0 ...
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bioRxiv - Immunology 2022Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific) and incubated for 30 min on a rotator at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... siPALS1 (5’-UUCCUUAUGAUGAACUGGCtt-3’) and siPATJ (5’-CCAGAUACUCACACUUCAGtt-3’, Ambion), siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’ ...
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bioRxiv - Biochemistry 2023Quote: ... DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (Invitrogen, D383) was used ...
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bioRxiv - Developmental Biology 2023Quote: ... mounting 3 dpf embryos in 3% methylcellulose (Thermo Scientific, 258111000). After imaging ...
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bioRxiv - Immunology 2020Quote: ... the cells were stained for 1h with the LIVE/DEAD™ fixable near-infrared dead cell stain (1:1000 dilution, Invitrogen) along with the following labelled antibodies diluted in an ice-cold staining buffer (PBS supplemented with 1% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... The following secondary antibodies were added for 1h at room temperature: anti-rabbit IgG-647 (1:500, Life technologies, A-21244), anti-rabbit IgG-488 (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: ... and incubated for 1h at room temperature with an Alexa 488 or 594 conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, MA). Approximately 60% of cells were MBP+ after 6 days of differentiation.
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bioRxiv - Genomics 2020Quote: ... Transformed cells were recovered in S.O.C media (from ElectroMAX Stabl4 kit) for 1h and spread on Luria–Bertani agar plates (245×245 mm, Thermo Fisher Scientific) containing 100 µg/mL ampicillin ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were washed three times using PBS and incubated for 1h at RT with AlexaFluor-conjugated secondary antibodies (Life Technologies). Cells were then mounted with Mowiol® for 30 min at 37°C and Images taken with a Carl Zeiss LSM780 laser scanning confocal microscope equipped with 100×1.40 oil plan-Apochromat objective (Zeiss) ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by washing with PBS three times and incubating for 1h at RT in blocking buffer containing Alexa-Fluor-conjugated isotype-specific secondary antibodies (Thermo Fisher). Cells were washed three times with PBS and DAPI (0.4 μg/ml in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10-20 µg of protein were loaded into 4-12% Bis-Tris Bolt gel and run at 150-200V constant for 1h in Bolt MOPS/MES 1X running buffer (Thermo Scientific). Dry transfer of the proteins to a nitrocellulose membrane was done using IBLOT2 (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... 6d ovaries underwent 1h of EdU labelling based on the protocol of the Click-iT™ Plus EdU Cell Proliferation Kit for Imaging (Invitrogen). Both 6d and 12d ovaries were stained for Fasciclin III (Fas3 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were allowed to adhere for 4 hours (+/-1h) before transfection with individual siRNAs (10nM) diluted in a mix of OptiMEM (Life Technologies) and 0.05μL of Interferin reagent (Polyplus Transfection) ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat antibody conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/500 in PBS ...
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bioRxiv - Genomics 2019Quote: ... 500ng of double stranded DNA template were used in 20μl IVT reactions for 1h using the TranscriptAid T7 High Yield Transcription Kit (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Invitrogen #A18805), goat anti-human IgM (Invitrogen #A18835) ...
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bioRxiv - Physiology 2019Quote: ... immuno-reactive proteins were blotted with anti-rabbit or goat horseradish peroxidase-labeled secondary antibodies for 1h at room temperature and revealed by enhanced chemiluminescence reagent (SuperSignal West Femto, Thermo Scientific), visualized using ChemiDoc MP Imaging System and data analyzed using the ImageLab 4.2 version software (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies/stains were diluted in 2% BSA PBS and samples were incubated for 1h and coverslips were subsequently washed and mounted with ProLong Gold anti-Fade moutant (Thermofisher, P36934). Localisations were visualised on a Ti-E Nikon microscope or Leica SP5 Confocal.
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bioRxiv - Genetics 2021Quote: ... Peptides were resuspended in 0.1% FA and were separated using a 1h gradient on an nLC coupled to a Q Exactive Plus tandem mass spectrometer (Thermo scientific) or an LTQ orbitrap XL mass spectrometer ...
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bioRxiv - Pathology 2020Quote: ... (Extended Data Table 1) and corresponding fluorescently conjugated secondary antibodies (1h at RT) together with 10 μg/ml Hoechst 33342 (trihydrochloride, trihydrate, ThermoFisher Scientific). Sections were mounted with ProLong Gold Antifade mounting medium ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies (anti-mouse nsP2 and E2 mAbs) were then added for 1h followed by incubation with Alexa Fluor (AF) 488-conjugated chicken anti-mouse antibody (Invitrogen, USA). Approximately 1×104 cells were acquired by the LSR-Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... 1h in Towbin buffer (25mM Tris, 192mM glycine) with 20% methanol) in a wet tank system (Mini Blot Module, Thermo Fisher). The membranes were blocked in 5% dry milk powder in TBS-T (5mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed and incubated for 1h at RT with 1:200 Alexa-fluor secondary antibodies: goat anti-rabbit 488 (Life Technologies), goat anti-rat 568 (Life Technologies ...