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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 6' ETHYL SPIRO 1 3 DIOXANE 2 3' INDOLIN 2' ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Grids were blotted for 2 - 6 s in a Vitrobot (Mark IV, Thermo Fisher Scientific) at 4 °C and 100% humidity ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl cDNA (1000 ng/ 100 μl) and 6 μl nuclease-free water (Thermo Scientific). qRT-PCR cycles included pre-incubation at 95°C for 5 minutes followed by 40 cycles of 95°C for 10 seconds 60°C for 30 seconds and 72°C for 30 seconds ...
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bioRxiv - Cell Biology 2024Quote: After mounting the cells using ProLong Diamond with 4’,6’-diamidino-2-phenylindole (DAPI) (Invitrogen), they were imaged using a DeltaVision II microscope system with an Olympus Ix-71 inverted microscope ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed and stained for 5min with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher) for detection of nuclei and mounted with Fluoromount-G (SouthernBiotech) ...
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bioRxiv - Immunology 2024Quote: ... as well as live/dead cells using 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher). In the case of intracellular stainings ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2ng/ml; Molecular Probes). Sections were mounted using ProLong Gold anti-fade reagent (ThermoFisher) ...
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bioRxiv - Genomics 2023Quote: ... slides were stained in 300 nM 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, D1306) and mounted in ProLong Diamond antifade mountant (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were counterstained with 4’,6-diamino-2-phenylindole (Prolong Gold antifade reagent DAPI; Invitrogen). The primary antibodies were vimentin ...
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bioRxiv - Neuroscience 2022Quote: ... A nuclear counterstaining was performed using 4ʹ,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) in TBS for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and mounted with Prolong Gold Antifade with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, P36935) as a counterstain for nuclei ...
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bioRxiv - Biochemistry 2023Quote: ... Nuclear counterstain was performed with 4’,6-diamidino-2-phenylindole (DAPI) fluorescent stain (Invitrogen, P36935). Cells were analyzed by confocal microscopy (LSM700 laser scanning microscope ...
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bioRxiv - Microbiology 2023Quote: ... The cell nuclei were stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (Life Technologies) at a dilution of 1:5000 in 1X PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were further incubated with DAPI (4’,6-diamidino-2-phenylindole, 5µg/ml, Invitrogen D1306) to stain nuclei prior to imaging ...
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bioRxiv - Bioengineering 2023Quote: ... 50 mg Sulfo-SANPAH (sulfosuccinimidyl 6-(4’-azido-2’- nitrophenylamino)hexanoate) (cat. 22589, ThermoFisher Scientific) was dissolved in 1 ml DMSO and diluted 25-times in Milli-Q water ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were stained with 4’,6-Diamidino-2-Phenylindole (DAPI, 5µg/ml, DAPI, ThermoFisher D1306) in PBS for 5 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were mounted with ProLong diamond with 4’,6’-diamidino-2-phenylindole (DAPI) (P36931; Invitrogen) and imaged on an Axio Observer 7 equipped with an Axiocam 702 mono camera and Zen 2.6 Pro software (Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... samples were also counterstained with DAPI (4′,6-diamidino-2-phenylindole; ThermoFisher Scientific, Waltham, MA) to visualize nuclei and/or CellMask (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... MOI coverslips were incubated with 300 nM 4’ 6-diamidino-2-phenylindole (DAPI; [D1306] Invitrogen) in PBS for 5 min ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis was evaluated thanks to 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies, Carlsbad, USA) and Annexin-V-APC (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... and stained with 200 ng/mL 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific, 62247) for 2 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
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bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
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bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
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bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Biophysics 2021Quote: ... Secondary antibodies were goat anti-mouse-Star red (Abberior, 2-0002-011-2) and goat anti-rabbit Star 580 (Abberior 2-0012-0050-8) (Life Technologies, 1:100).
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bioRxiv - Microbiology 2021Quote: ... a sequencing reaction was performed with 2 pmol of unmodified RNA and 2 µl of a 2 mM AS primer 1 labeled with Ned (Life Technologies SAS, France). Reverse transcription was performed as for the SHAPE (+ ...
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bioRxiv - Neuroscience 2022Quote: RNA was 3’ labelled with biotin using Pierce™ RNA 3’ End Biotinylation Kit (ThermoFisher) and purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... Head kidney pieces (3 mm × 3 mm) were collected in RNA later (Thermo Fisher Scientific), transferred to 500 μL MagNA Pure LC RNA Isolation Tissue Lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Gdf1/3 and Gdf1/3-like mRNA were synthesized using T7 mMESSAGE mMACHINE kit (Ambion). Unfertilized eggs were injected with mRNAs and fertilized as previously described50 ...
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bioRxiv - Biophysics 2023Quote: ... 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine (DiI) and 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine (DiD)) were purchased from ThermoFisher Scientific and used without additional purification ...
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed following 3 × 3 min incubations with ACK Lysing Buffer (Gibco). The remaining white blood cells were washed with PBS twice and plated for surface staining ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was stained with To-Pro-3 (1/200 dilution, 1 mM, Invitrogen T3605).
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bioRxiv - Neuroscience 2022Quote: ... dried and coverslipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Primary antibodies with the following dilutions used in this study include ...
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bioRxiv - Biochemistry 2024Quote: ... columns were washed with 3 column volumes (CV) of 1:1 RNase ZAP (Ambion) followed by 3 CV nuclease-free water (DEPC-treated MilliQ water) ...
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bioRxiv - Pathology 2024Quote: ... and 3-hydroxybutyrate dehydrogenase 1 (15417-1-AP, Thermo Fisher Sci, Waltham, MA, USA). HRP-conjugated secondary antibodies (NA9340 ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500; 2 mg/mL Alexa Fluor 568, Invitrogen, 1:1000) diluted in PBS for 1.5 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 1 hour with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were split at 1:2 every 2 days using Trypsin-EDTA (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2019Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 2 h with occasional agitation ...
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bioRxiv - Physiology 2023Quote: ... cardiomyocytes were incubated with 1 µM Fura-2- acetoxymethyl ester (Fura-2 AM, cell permeant, ThermoFisher) and 0.075% Pluronic F-127 (20% in DMSO ...
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bioRxiv - Biochemistry 2021Quote: ... Stained ovaries were finally mounted with one drop of vectashield in epoxy diagnostic slides (Thermo-Fisher Scientific, 3 wells 14 mm) and covered with high precision cover glasses ...
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bioRxiv - Microbiology 2024Quote: ... isolated RNA was run using a TaqMan Fast Virus One-Step Master Mix (applied Biosystems) on a QuantStudio 3 Flex Real-Time PCR system (Applied Biosystems). RNA standards with known copy numbers were used to generate a standard curve and calculate sample copy numbers ...